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Pierce™ High Sensitivity Streptavidin-HRP Thermo Scientific™

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 0.5 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

Related Products
Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted

Streptavidin Protein, AP Invitrogen™

Streptavidin for Western Blot, ELISA

Pierce™ Streptavidin Poly-HRP Thermo Scientific™

Thermo Scientific Pierce Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

Features of Streptavidin Poly-HRP:

High sensitivity—detect low-abundant targets (low picogram-femtogram range) with high signal-to-noise ratios
Robust—consistent manufacturing and purification to eliminate low- and un-conjugated molecules for minimal background and high sensitivity
Flexible—compatible with chromogenic, fluorogenic and chemiluminescent substrates
Versatile—compatible with ELISA, Western blotting and immunohistochemistry (IHC)
Easy to use—can be directly substituted into immunoassays and other detection assays
Convenient—ready-to-use stabilized liquid format stored at 4°C
Cost effective—requires less conjugate per assay and standard HRP conjugates

Streptavidin Poly-HRP conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. Streptavidin Poly-HRP is purified to remove unconjugated streptavidin molecules that reduce signal intensity by competing for binding sites with horseradish peroxidase (HRP)-conjugates molecules. In addition, the conjugate is devoid of HRP monomers that can cause background signal. The Pierce Streptavidin Poly-HRP is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.

Several forms of streptavidin-HRP conjugates are available that do not contain polymerized HRP (poly-HRP) and are suitable for most biotin detection assays. These and other commonly used HRP-conjugated antibodies and biotin-binding proteins are limited to one to three HRP molecules per protein in order to maintain functionality of the conjugate. This molar ratio of conjugation is effective in routine experiments but may lack the sensitivity required for detecting targets present in low picogram to femtogram amounts without the use of additional steps to amplify the signal.

When a higher degree of signal amplification is needed with standard HRP conjugates, methods such as peroxidase anti-peroxidase (PAP) systems or avidin-biotin complexes (ABC) can be used. However, these methods increase the cost of the assay and the number of steps that must be optimized. Pierce Streptavidin Poly-HRP circumvents such problems because it uses polymers of HRP molecules that are conjugated to streptavidin in a proprietary manufacturing process that increases the molar ratio of HRP on the conjugate while maintaining functionality. The increased molar ratio of HRP molecules to streptavidin results in more HRP molecules being available per binding event to react with the substrate. Therefore, Pierce Streptavidin Poly-HRP provides substantial signal amplification over other HRP-conjugates, eliminating the need for additional steps and saving time and expense.

As with all immunoassays, optimization is required to achieve the best signal-to-noise ratio. Switching to higher sensitivity substrates and conjugates can reveal more desired signal as well as background signal. If this occurs, follow standard primary and secondary dilution techniques or perform blocking buffer optimization.

Streptavidin, Marina Blue™ conjugate Invitrogen™

Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

Streptavidin, Alexa Fluor™ 647 Conjugate Invitrogen™

Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 647 Streptavidin Conjugates:
Alexa Fluor® 647 streptavidin conjugate has Ex/Em maxima of ~ (650/668)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin, (PE-Cy5.5) Invitrogen™

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin R PE Invitrogen™

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: RPE Conjugate

Streptavidin Protein, DyLight 650 Invitrogen™

Streptavidin for IF

eBioscience™ Streptavidin PE-Cyanine7 Conjugate Invitrogen™

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser, Green Laser, Yellow-Green Laser

Emit
775 nm

Excite
488 - 561 nm

Reported Application
Flow Cytometric Analysis

Streptavidin Protein, AP Thermo Scientific™

Thermo Scientific™ Streptavidin, Alkaline Phosphatase Conjugate includes streptavidin in a purified form, conjugated to alkaline phosphatase for substrate based detection.

Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Streptavidin, Alkaline Phosphatase Conjugate enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.

Recommended concentrations: ELISA (0.1 µg/mL), immunofluorescence (assay dependent), western blot (2 µg/mL)

eBioscience™ Streptavidin Super Bright 600 Conjugate Invitrogen™

eBioscience Streptavidin Super Bright 600 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 600 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Clone specific performance after fixation should be determined empirically.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

Streptavidin, Allophycocyanin, crosslinked, conjugate Invitrogen™

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin. Allophycocyanin is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted Thermo Scientific™

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 2 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 2 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

Related Products
Pierce™ High Sensitivity Streptavidin-HRP

Streptavidin-Horseradish Peroxidase (HRP) Conjugate Invitrogen™

Streptavidin-HRP conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunoblotting, ELISA, immunomicroscopy. The amount of conjugate provided is sufficient for 25 western blots using 10 ml of working solution per reaction or 25 immunostaining reactions using 1 ml of working solution per reaction as appropriate.

eBioscience™ Streptavidin Super Bright 436 Conjugate Invitrogen™

eBioscience Streptavidin Super Bright 436 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 436 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

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