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Streptavidin - HRP (Invitrogen™)

Reactivity: HRP-SA reacts with biotin.

Applications: Immunohistochemistry, Western blotting, and ELISA

Streptavidin, (HRPO) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS

Streptavidin HRP (ELISA GD) (Invitrogen™)

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate (ELISA grade)

Streptavidin, horseradish peroxidase conjugate (Invitrogen™)

The streptavidin, horseradish peroxidase (HRP) conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic HRP substrates, such as the tyramide signal amplification (TSA) technology.

Pierce™ Streptavidin Poly-HRP (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

Features of Streptavidin Poly-HRP:

High sensitivity—detect low-abundant targets (low picogram-femtogram range) with high signal-to-noise ratios
Robust—consistent manufacturing and purification to eliminate low- and un-conjugated molecules for minimal background and high sensitivity
Flexible—compatible with chromogenic, fluorogenic and chemiluminescent substrates
Versatile—compatible with ELISA, Western blotting and immunohistochemistry (IHC)
Easy to use—can be directly substituted into immunoassays and other detection assays
Convenient—ready-to-use stabilized liquid format stored at 4°C
Cost effective—requires less conjugate per assay and standard HRP conjugates

Streptavidin Poly-HRP conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. Streptavidin Poly-HRP is purified to remove unconjugated streptavidin molecules that reduce signal intensity by competing for binding sites with horseradish peroxidase (HRP)-conjugates molecules. In addition, the conjugate is devoid of HRP monomers that can cause background signal. The Pierce Streptavidin Poly-HRP is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.

Several forms of streptavidin-HRP conjugates are available that do not contain polymerized HRP (poly-HRP) and are suitable for most biotin detection assays. These and other commonly used HRP-conjugated antibodies and biotin-binding proteins are limited to one to three HRP molecules per protein in order to maintain functionality of the conjugate. This molar ratio of conjugation is effective in routine experiments but may lack the sensitivity required for detecting targets present in low picogram to femtogram amounts without the use of additional steps to amplify the signal.

When a higher degree of signal amplification is needed with standard HRP conjugates, methods such as peroxidase anti-peroxidase (PAP) systems or avidin-biotin complexes (ABC) can be used. However, these methods increase the cost of the assay and the number of steps that must be optimized. Pierce Streptavidin Poly-HRP circumvents such problems because it uses polymers of HRP molecules that are conjugated to streptavidin in a proprietary manufacturing process that increases the molar ratio of HRP on the conjugate while maintaining functionality. The increased molar ratio of HRP molecules to streptavidin results in more HRP molecules being available per binding event to react with the substrate. Therefore, Pierce Streptavidin Poly-HRP provides substantial signal amplification over other HRP-conjugates, eliminating the need for additional steps and saving time and expense.

As with all immunoassays, optimization is required to achieve the best signal-to-noise ratio. Switching to higher sensitivity substrates and conjugates can reveal more desired signal as well as background signal. If this occurs, follow standard primary and secondary dilution techniques or perform blocking buffer optimization.

Streptavidin HRP (Invitrogen™)

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate

Streptavidin Protein, HRP (Thermo Scientific™)

Thermo Scientific™ Pierce™ Streptavidin, Horseradish Peroxidase Conjugate includes streptavidin in a purified form conjugated to peroxidase for substrate-based detection.

Streptavidin, Horseradish Peroxidase Conjugate enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including western blotting, ELISA, immunohistochemistry, and fluorescence imaging. Streptavidin, Horseradish Peroxidase conjugate is supplied as lyophilized powder in phosphate-based buffer for immediate reconstitution with water.

Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Pierce streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Recommended for use in ELISA (0.1 µg/mL), immunohistochemistry (1-10 µg/mL), and western blot (2 µg/mL) applications.

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HRP-Conjugated Streptavidin (Thermo Scientific™)

HRP-Conjugated Streptavidin consists of streptavidin protein that is covalently conjugated to horseradish peroxidase (HRP) enzyme (RZ > 3.0). Streptavidin binds to biotin and the conjugated HRP provides enzyme activity for detection using an appropriate substrate system. This particular product has been used primarily in sandwich ELISA applications to provide consistent measurement of biotinylated detection antibodies.

Features of HRP-Conjugated Streptavidin :

Product Number: N100
Size: 0.25 mL
Concentration: 1.25 mg/mL
Formulation: Provided in a conjugate stabilizer containing 0.1% ProClin Compound as a preservative.
Storage: Shipped on cold packs. Store at 2-8°C upon receipt. Protect from exposure to light. Stable until expiration date if stored under these conditions.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is from 1:4,000 to 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

Streptavidin-Horseradish Peroxidase (HRP) Conjugate (Invitrogen™)

Streptavidin-HRP conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunoblotting, ELISA, immunomicroscopy. The amount of conjugate provided is sufficient for 25 western blots using 10 ml of working solution per reaction or 25 immunostaining reactions using 1 ml of working solution per reaction as appropriate.

Pierce™ High Sensitivity Streptavidin-HRP (Thermo Scientific™)

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 0.5 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

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Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted

Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted (Thermo Scientific™)

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 2 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 2 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

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Poly-HRP Streptavidin (Thermo Scientific™)

Poly-HRP Streptavidin consists of streptavidin protein that is covalently conjugated to a polymerized form of horseradish peroxidase (HRP) enzyme. Streptavidin binds to biotin and the conjugated HRP polymer provides a high level of activity for detection using an appropriate substrate system. This particular Poly-HRP Streptavidin product has been used primarily in sandwich ELISA applications to improve the signal provided by typical (non-Poly) streptavidin-HRP conjugates.

Features of Poly-HRP Streptavidin:

Formulation: Conjugate in PBS containing distilled glycerol, BSA and a preservative.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is 1:2,000 - 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

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Ultra Streptavidin-HRP (Thermo Scientific™)

Streptavidin-HRP consists of streptavidin protein that is covalently conjugated to horseradish peroxidase (HRP) enzyme. Streptavidin binds to biotin and the conjugated HRP provides a high level of activity for detection using an appropriate substrate system.

Features of Streptavidin-HRP:

Product Number: N504
Size: 1.5 mL
Formulation: Conjugate in stabilizer solution containing protein and preserved with 0.02% methyliso-thiazolone, 0.02% bromonitrodioxane, and 0.002% other active isothiazolones.
Storage: Shipped on cold packs. Store at 2-8°C. Stable until expiration date if stored under these conditions.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is 1:400 - 1:2,000 in ELISA diluent (e.g., PBS containing 4% protease-free BSA) . The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.