Shop All Streptavidin & Conjugates

Streptavidin, Unconjugated Invitrogen™

Purified from Streptomyces avidinii and provided in lyophilized form. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: Unconjugated

Streptavidin, Alexa Fluor™ 546 conjugate Invitrogen™

Alexa Fluor® 546 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 546 Streptavidin Conjugates:
Alexa Fluor® 546 streptavidin conjugate has Ex/Em maxima of ~ (556/573)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin HRP Invitrogen™

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate

eBioscience™ Streptavidin eFluor™ 450 Conjugate Invitrogen™

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Violet Laser

Emit
445 nm

Excite
405 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, Alexa Fluor™ 568 conjugate Invitrogen™

Alexa Fluor® 568 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 568 Streptavidin Conjugates:
Alexa Fluor® 568 streptavidin conjugate has Ex/Em maxima of ~ (578/603)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin R PE Invitrogen™

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: RPE Conjugate

Streptavidin, (PE-Cy7) Invitrogen™

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin, Alexa Fluor™ 594 conjugate Invitrogen™

Alexa Fluor® 594 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 594 Streptavidin Conjugates:
Alexa Fluor® 594 streptavidin conjugate has Ex/Em maxima of ~ (590/617)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin HRP (ELISA GD) Invitrogen™

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate (ELISA grade)

eBioscience™ Streptavidin APC-eFluor™ 780 Conjugate Invitrogen™

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Red Laser

Emit
780 nm

Excite
633 - 647 nm

Reported Application
Flow Cytometric Analysis

Streptavidin Invitrogen™

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin.

Capacity: Binding activity of streptavidin is determined to be >11 units per mg protein. One unit of binding activity is defined as that amount of protein which binds 1 µg of biotin (Green, N.M., Biochem. J., 89:599,1963)

Form: Streptavidin is provided in lyophilized form in 10 mM phosphate buffered saline, pH 7.4. Reconstitute with 1 ml of distilled or deionized water.

Storage: Store at 4°C.

Streptavidin, (PE-TR) Invitrogen™

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin, Allophycocyanin Conjugate, premium grade Invitrogen™

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than does avidin. Allophycocyanin is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm. Our streptavidin allophycocyanin conjugate *premium grade* is a further fractionation of our S-868 product, intended especially for applications that benefit from additional purity.

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin, Alexa Fluor™ 430 conjugate Invitrogen™

Our Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ~434/539 nm). Streptavidin, a nonglycosylated biotin-binding protein with a near-neutral isoelectric point, reportedly exhibits less nonspecific binding than avidin.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin Protein, DyLight 594 Invitrogen™

Streptavidin for Western Blot, IF, ICC, IHC, IP, ELISA
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