Shop All Streptavidin & Conjugates

Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted (Thermo Scientific™)

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 2 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 2 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

Related Products
Pierce™ High Sensitivity Streptavidin-HRP

Streptavidin-CY3 (ZyMax Grade) (Invitrogen™)

Applications: Immunohistochemistry, Western blotting, ELISA, ISH

ZyMAX™ reagents offer enhanced assay sensitivity, higher titer, and greater flexibility in assay design than standard grade reagents.

Pierce™ Streptavidin (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin is a purified unconjugated recombinant protein form.

Features of Streptavidin:

Recombinant streptavidin—high-quality protein, 53kDa, pI = 6.8 to 7.5
Pure protein —choose pure, unconjugated protein for biotin blocking and controls

The purified recombinant Streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin is supplied as lyophilized in phosphate-based buffers for immediate reconstitution with water.

Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Related Products
Pierce™ Streptavidin, Horseradish Peroxidase Conjugated
Pierce™ Streptavidin, Alkaline Phosphatase Conjugated
Pierce™ Streptavidin, Maleimide-Activated
Pierce™ Streptavidin, Hydrazide-Activated

Streptavidin - HRP (Invitrogen™)

Reactivity: HRP-SA reacts with biotin.

Applications: Immunohistochemistry, Western blotting, and ELISA

Streptavidin, (R-PE) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

LanthaScreen™ Tb-Streptavidin, 1 mg/mL Kit

As part of the LanthaScreen® TR-FRET Toolbox of assay reagents, LanthaScreen® Tb-labeled streptavidin is a "generic" reagent that can be used to easily label biotinylated biomolecules with terbium for use in TR-FRET assays. Although the terbium chelate donor can be directly attached to a biomolecule such as a protein or peptide, the Tb-labeled streptavidin can be "indirectly" attached to a target molecule through a biotin-mediated association with Tb-labeled streptavidin.

Streptavidin-CY5 (ZyMax Grade) (Invitrogen™)

Applications: Western blotting, ELISA, Immunohistochemistry, ISH

ZyMAX™ reagents offer enhanced assay sensitivity, higher titer, and greater flexibility in assay design than standard grade reagents.

Poly-HRP Streptavidin (Thermo Scientific™)

Poly-HRP Streptavidin consists of streptavidin protein that is covalently conjugated to a polymerized form of horseradish peroxidase (HRP) enzyme. Streptavidin binds to biotin and the conjugated HRP polymer provides a high level of activity for detection using an appropriate substrate system. This particular Poly-HRP Streptavidin product has been used primarily in sandwich ELISA applications to improve the signal provided by typical (non-Poly) streptavidin-HRP conjugates.

Features of Poly-HRP Streptavidin:

Formulation: Conjugate in PBS containing distilled glycerol, BSA and a preservative.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is 1:2,000 - 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

Related Products
Poly-HRP Dilution Buffer

Streptavidin Protein, PE (Invitrogen™)

Streptavidin for Western Blot, IHC, ELISA

LanthaScreen™ Eu-Streptavidin

A general reagent for use in assay development, the Europium-Streptavidin binds to biotinylated peptide or physiological protein substrates.

Streptavidin (Invitrogen™)

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin.

Capacity: Binding activity of streptavidin is determined to be >11 units per mg protein. One unit of binding activity is defined as that amount of protein which binds 1 µg of biotin (Green, N.M., Biochem. J., 89:599,1963)

Form: Streptavidin is provided in lyophilized form in 10 mM phosphate buffered saline, pH 7.4. Reconstitute with 1 ml of distilled or deionized water.

Storage: Store at 4°C.

Qdot™ 585 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 585 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

Streptavidin Protein, DyLight 650 (Invitrogen™)

Streptavidin for IF

Streptavidin, R-Phycoerythrin Conjugate (SAPE) - 1 mg/mL (Invitrogen™)

Streptavidin is a bacterially derived biotin-binding protein that exhibits less nonspecific binding than avidin. Phycoerythrin also known as R-Phycoerythrin (RPE) is an intensely bright phycobiliprotein isolated from red algae with absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm. Used in flow, microplate assays and microarrays, this has remained one of our top three selling products for decades—a proven performer that you can trust.

Important Features of Streptavidin RPE
• Biotin Detection with high quantum efficiency photoprotein
• 5–10 times brighter than organic fluors
• High water solubility
• Ideal for Flow, microarrays and microplate assays
• Also available as Premium grade with higher purity confirmed by HPLC (S21388)

Streptavidin and RPE
The high affinity of avidin, an egg-white protein, for biotin was first exploited in histochemical applications in the mid-1970s. Avidin, and its bacterial counterpart, streptavidin (from Streptomy cesavidinii), have since become standard reagents for diverse detection schemes. Streptavidin, a nonglycosylated 52,800-dalton protein, exhibits less nonspecific binding than avidin.

Rhodophyta Phycoerythrin (RPE) has a MW of 240,000 Daltons and has a quantum efficiency of 78% making it an incredibly bright reagent. However, the photostability is extremely poor and is not traditionally recommended for microscopy. But the broad absorption and bright orange signal may it ideal for flow, microplate and microarray applications whenever sensitive detection of biotins is required.

Learn more about avidin biotin detection in general

Learn more about SAV-RPE

Pierce™ High Sensitivity Streptavidin-HRP (Thermo Scientific™)

Thermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity Streptavidin-HRP, 0.5 mL:

High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. Experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted 8-to 10-fold more than traditional HRP conjugates and still produce similar results.

Related Products
Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted

Pierce™ Streptavidin Poly-HRP (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

Features of Streptavidin Poly-HRP:

High sensitivity—detect low-abundant targets (low picogram-femtogram range) with high signal-to-noise ratios
Robust—consistent manufacturing and purification to eliminate low- and un-conjugated molecules for minimal background and high sensitivity
Flexible—compatible with chromogenic, fluorogenic and chemiluminescent substrates
Versatile—compatible with ELISA, Western blotting and immunohistochemistry (IHC)
Easy to use—can be directly substituted into immunoassays and other detection assays
Convenient—ready-to-use stabilized liquid format stored at 4°C
Cost effective—requires less conjugate per assay and standard HRP conjugates

Streptavidin Poly-HRP conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. Streptavidin Poly-HRP is purified to remove unconjugated streptavidin molecules that reduce signal intensity by competing for binding sites with horseradish peroxidase (HRP)-conjugates molecules. In addition, the conjugate is devoid of HRP monomers that can cause background signal. The Pierce Streptavidin Poly-HRP is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.

Several forms of streptavidin-HRP conjugates are available that do not contain polymerized HRP (poly-HRP) and are suitable for most biotin detection assays. These and other commonly used HRP-conjugated antibodies and biotin-binding proteins are limited to one to three HRP molecules per protein in order to maintain functionality of the conjugate. This molar ratio of conjugation is effective in routine experiments but may lack the sensitivity required for detecting targets present in low picogram to femtogram amounts without the use of additional steps to amplify the signal.

When a higher degree of signal amplification is needed with standard HRP conjugates, methods such as peroxidase anti-peroxidase (PAP) systems or avidin-biotin complexes (ABC) can be used. However, these methods increase the cost of the assay and the number of steps that must be optimized. Pierce Streptavidin Poly-HRP circumvents such problems because it uses polymers of HRP molecules that are conjugated to streptavidin in a proprietary manufacturing process that increases the molar ratio of HRP on the conjugate while maintaining functionality. The increased molar ratio of HRP molecules to streptavidin results in more HRP molecules being available per binding event to react with the substrate. Therefore, Pierce Streptavidin Poly-HRP provides substantial signal amplification over other HRP-conjugates, eliminating the need for additional steps and saving time and expense.

As with all immunoassays, optimization is required to achieve the best signal-to-noise ratio. Switching to higher sensitivity substrates and conjugates can reveal more desired signal as well as background signal. If this occurs, follow standard primary and secondary dilution techniques or perform blocking buffer optimization.

eBioscience™ Streptavidin Super Bright 436 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 436 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 436 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

eBioscience™ Streptavidin FITC Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser

Emit
520 nm

Excite
488 nm

Reported Application
Flow Cytometric Analysis, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections

Streptavidin Protein, DyLight 594 (Invitrogen™)

Streptavidin for Western Blot, IF, ICC, IHC, IP, ELISA

Streptavidin, (APC) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Streptavidin, Alexa Fluor™ 647—R-Phycoerythrin Conjugate (Alexa Fluor™ 647—R-Phycoerythrin Streptavidin) (Invitrogen™)

The streptavidin, Alexa Fluor™ 647-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ~496,565/667 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications using a single excitation wavelength, 488 nm. The Alexa Fluor™ 647 conjugates of R-PE have emission spectra almost identical to those of Cy5-R-PE conjugates but tend to have more intesne long-wavelength emission and to require less compensation in the R-PE channel.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Qdot™ 800 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 800 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

Streptavidin Protein, DyLight 680 (Invitrogen™)

Streptavidin for Western Blot, IF, ICC, IHC, IP, ELISA

Streptavidin, Alexa Fluor™ 790 Conjugate (Invitrogen™)

Alexa Fluor® 790 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 790 Streptavidin Conjugates:

Alexa Fluor® 790 streptavidin conjugate has Ex/Em maxima of ~(782/805)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates—Section 7.6 in the Molecular Probes Handbook

eBioscience™ Streptavidin eFluor™ 615 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in immunohistochemistry and immunocytochemistry applications. Streptavidin binds to biotin with high affinity.

Emit
615 nm

Excite
595 nm

Reported Application
Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

Streptavidin, Allophycocyanin, crosslinked, conjugate (Invitrogen™)

Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin. Allophycocyanin is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

eBioscience™ Streptavidin PerCP-Cyanine5.5 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser

Emit
695 nm

Excite
488 nm

Reported Application
Flow Cytometric Analysis

Streptavidin Protein, Rhodamine (Invitrogen™)

Streptavidin for Western Blot, IHC, ELISA

Streptavidin, Rhodamine Red™-X conjugate (Invitrogen™)

The streptavidin, Rhodamine-Red™-X conjugate has excitation and emission maxima ~570/590 and is widely used to detect biotinylated probes by fluorescence microscopy.

Alexa Fluor™ 488 Streptavidin, 5 nm colloidal gold conjugate (Invitrogen™)

Alexa Fluor® 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 488 Streptavidin Conjugates:
Alexa Fluor® 488 streptavidin conjugate has Ex/Em maxima of ~ (495/519)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

eBioscience™ Streptavidin eFluor™ 570 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, immunohistochemistry, and immunocytochemistry applications. Streptavidin binds to biotin with high affinity.

Emit
570 nm

Excite
555 nm

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunohistochemical Staining of Frozen Tissue Sections, Immunocytochemistry

eBioscience™ Streptavidin eFluor™ 506 Conjugate (Invitrogen™)

eBioscience Streptavidin eFluor 506 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. eFluor organic dyes are traditional fluorescent dyes for use in multiple applications, including multicolor flow cytometry and immunofluorescence microscopy. To make fluorophore selection easier, all eFluor organic dyes are named after their emission wavelength, and they are fully compatible with most conventional dyes, fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

The eFluor 506 organic dye is a violet-laser excitable fluorophore that has an emission peak of 506 nm. This dye is optimized for use in flow cytometry and, when combined with all five Super Bright dye antibody conjugates, can provide six detection channels of the violet laser. We recommend using a 510/20 band pass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin eFluor 506 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. It can be used ≤ 0.125 μg per test. A test is defined as the amount (μg) of streptavidin fluorochrome conjugate that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Qdot™ 605 Streptavidin Conjugate (Invitrogen™)

Qdot® 605 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 μM solutions.

Important Features of Qdot® Streptavidin Conjugates:

• Streptavidin Qdot® 605 conjugate has emission maxima of ~605 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.

• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

Related Links:
Learn more about Avidin-Biotin Detection
To learn more about Qdot® Nanocrystals

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

eBioscience™ Streptavidin PE-eFluor™ 610 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, immunohistochemistry, and immunocytochemistry applications. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser, Green Laser, Yellow-Green Laser

Emit
607 nm

Excite
488 - 561 nm

Reported Application
Flow Cytometric Analysis

Qdot™ 655 ITK™ Streptavidin Conjugate Kit (Invitrogen™)

The Qdot® 655 ITK™ Streptavidin Conjugate Kit provides the Qdot® streptavidin conjugate as 250 µL of a 2 µM solution. Unlike the Qdot® streptavidin conjugates, the Qdot® ITK™ streptavidin conjugates have the streptavidin covalently attached to the inner amphiphilic coating without a PEG linker. These Qdo® conjugates can be used for binding biotinylated probes. The kit also includes 30 mL of Qdot® incubation buffer.

Streptavidin, Alexa Fluor™ 430 conjugate (Invitrogen™)

Our Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ~434/539 nm). Streptavidin, a nonglycosylated biotin-binding protein with a near-neutral isoelectric point, reportedly exhibits less nonspecific binding than avidin.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin - FITC (Invitrogen™)

Reactivity: FITC-SA will react with biotin.

Streptavidin, Alexa Fluor™ 546 conjugate (Invitrogen™)

Alexa Fluor® 546 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 546 Streptavidin Conjugates:
Alexa Fluor® 546 streptavidin conjugate has Ex/Em maxima of ~ (556/573)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin, Pacific Blue™ conjugate (Invitrogen™)

Our streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~410/455 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Qdot™ 585 Streptavidin Conjugate (Invitrogen™)

Qdot® 585 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 μM solutions.

Important Features of Qdot® Streptavidin Conjugates:

• Streptavidin Qdot® 585 conjugate has emission maxima of ~585 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.

• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

Related Links:
Learn more about Avidin-Biotin Detection
To learn more about Qdot® Nanocrystals

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Qdot™ 565 Streptavidin Conjugate (Invitrogen™)

Qdot® 565 streptavidin conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 µM solutions.

Important Features of Qdot® Streptavidin Conjugates:
Streptavidin Qdot® 565 conjugate has emission maxima of ~565 nm
Approximately 5 to 10 streptavidins per Qdot® nanocrystal
Extremely photostable and bright fluorescence
Efficiently excited with single-line excitation sources
Narrow emission, large Stokes shift
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

To learn more about Qdot® Nanocrystals

Streptavidin, (HRPO) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS

Streptavidin, horseradish peroxidase conjugate (Invitrogen™)

The streptavidin, horseradish peroxidase (HRP) conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic HRP substrates, such as the tyramide signal amplification (TSA) technology.

eBioscience™ Streptavidin eFluor™ 450 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Violet Laser

Emit
445 nm

Excite
405 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin) (Invitrogen™)

The streptavidin, Alexa Fluor™ 750-allophycocyanin (APC) streptavidin tandem conjugate has excitation and emission maxima at ~650/779 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications following excitation at 633 nm or 647 nm.

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin Protein, AP (Invitrogen™)

Streptavidin for Western Blot, ELISA

eBioscience™ Streptavidin APC-eFluor™ 780 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Red Laser

Emit
780 nm

Excite
633 - 647 nm

Reported Application
Flow Cytometric Analysis

Streptavidin HRP (Invitrogen™)

Purified from Streptomyces avidinii and provided in a phosphate buffered saline solution. The streptavidin/avidin-biotin staining and detection strategy is used when increased assay sensitivity is desired for a wide variety of systems.

Format/Formulation: HRP Conjugate

eBioscience™ Streptavidin PE-Cyanine5 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser, Green Laser, Yellow-Green Laser

Emit
667 nm

Excite
488 - 561 nm

Reported Application
Flow Cytometric Analysis

Qdot™ 705 Streptavidin Conjugate (Invitrogen™)

Qdot® 705 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 μM solutions.

Important Features of Qdot® Streptavidin Conjugates:

• Streptavidin Qdot® 705 conjugate has emission maxima of ~705 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.

• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

Related Links:
Learn more about Avidin-Biotin Detection
To learn more about Qdot® Nanocrystals

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Qdot™ 525 Streptavidin Conjugate (Invitrogen™)

Qdot® 525 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot® nanocrystal conjugates are provided as 1 μM solutions.

Important Features of Qdot® Streptavidin Conjugates:

• Streptavidin Qdot® 525 conjugate has emission maxima of ~525 nm
• Approximately 5 to 10 streptavidins per Qdot® nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors or try the Qdot® Streptavidin Sampler Kit, containing Qdot® streptavidin conjugates in six colors 525, 565, 585, 605, 655, and 705. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.

• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

Related Links:
Learn more about Avidin-Biotin Detection
To learn more about Qdot® Nanocrystals

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Streptavidin, R-Phycoerythrin Conjugate (SAPE) - premium grade - 1 mg/mL (Invitrogen™)

The R-phycoerythrin (R-PE) conjugate of streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (R-phycoerythrin). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Streptavidin R-PE is very valuable in biotin-streptavidin detection protocols developed for flow cytometry, microplate assays, and microarrays. This R-PE streptavidin conjugate is a high-purity product (purity confirmed by HPLC) and is supplied as 1 mL of a 1 mg/mL solution.

Important Features of Streptavidin RPE:
Ex/Em maxima of ~ (496, 546, 565/578) nm
5 to 10 times brighter than organic dyes
Very high solubility in aqueous solutions
Free of all unconjugated streptavidin; and mostly free of unconjugated R-PE
Ideal for flow cytometry, microplate assays, and microarrays

Properties of R-phycoerythrin
R-PE has a quantum efficiency of 78%, making it an incredibly bright reagent, useful where high sensitivity is needed. Because of poor photostability, however, it is not recommended for fluorescence microscopy.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about the practical applications for Streptavidin R-PE

Streptavidin, Alexa Fluor™ 680 Conjugate (Invitrogen™)

Alexa Fluor® 680 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 680 Streptavidin Conjugates:
Alexa Fluor® 680 streptavidin conjugate has Ex/Em maxima of ~ (679/702)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin, fluorescein conjugate (Invitrogen™)

Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.

Streptavidin, Alexa Fluor™ 647 conjugate (Invitrogen™)

Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 647 Streptavidin Conjugates:
Alexa Fluor® 647 streptavidin conjugate has Ex/Em maxima of ~ (650/668)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Streptavidin, (Cy5) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.

Streptavidin - AP (Invitrogen™)

Reactivity: AP-SA reacts with biotin.

Applications: Immunohistochemistry, Western blotting, and ELISA

eBioscience™ Streptavidin Super Bright 702 Conjugate (Invitrogen™)

eBioscience Streptavidin Super Bright 702 is a streptavidin-fluorochrome conjugate for indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name. They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation. Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents.

Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Applications tested
Streptavidin Super Bright 702 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies. The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest.

Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Clone specific performance after fixation should be determined empirically.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Complete Staining Buffer for more information.

Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.

eBioscience™ Streptavidin PerCP-eFluor™ 710 Conjugate (Invitrogen™)

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser

Emit
710 nm

Excite
488 nm

Reported Application
Flow Cytometric Analysis

Streptavidin, (FITC) (Invitrogen™)

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.