Shop All Cell, Tissue, & In Vivo Labeling Reagents

Click-iT™ Alexa Fluor™ 647 sDIBO Alkyne (Invitrogen™)

Click-iT Alexa Fluor 647 sDIBO Alkyne reacts with azides via a copper-free Click chemistry reaction to produce fluorescent Alexa Fluor 647 bioconjugates. sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less “sticky” and give lower signal background in biological samples. Copper-free Click bio-conjugation reactions are ideal for surface labeling of live cells and also minimize damage to enzymes and fluorescent proteins like GFP or R-PE. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be now be labeled easily, yielding more soluble bioconjugates with improved biological labeling utility.

• More soluble than DIBO cyclooctynes leading to more soluble conjugates
• Minimal background potential in cells and tissues compared to original DIBO cycloctynes

Learn more about Alexa Fluor dyes ›

CellVue™ Maroon Cell Labeling Kit (Invitrogen™)

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. The Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Maroon is a far-red fluorescent cell labeling reagent. It is optimally excited at 647 nm and has a peak emission of 667 nm; however, it can be detected equally in filter sets designed for APC, Alexa Fluor™ 700, and APC-eFluor™ 780 or similar fluorochromes. Therefore, using antibodies conjugated to these fluorochromes in combination with using CellVue™ Maroon is not recommended.

Reported Application
Microscopy, Cell Labeling, Immunocytochemistry, Flow Cytometric Analysis

CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry (Invitrogen™)

CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

• Superior performance—bright, single-peak staining enables visualization of multiple generations
• Long-term signal stability—well-retained in cells for several days post stain
• Non-toxic—staining does not adversely effect cell health
• Simple, robust staining protocol

View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry.

Superior fluorescent staining
Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ CFSE dye enables the visualization of eight or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. Consistent, homogeneous staining results in very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTrace™ CFSE dye easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye is designed to provide a consistent signal, even after several days in a cell culture environment.

Non-toxic
CellTrace™ CFSE dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells. Researchers have used CFSE SE labeling to show that transplantable hematopoietic cells proliferate in vitro in response to stimulation by a growth factor cocktail.

Simple, robust staining protocol
The CellTrace™ CFSE Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1 µL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.

CellTracker™ Blue CMF2HC Dye (Invitrogen™)

CellTracker™ Blue CMF2HC (4-chloromethyl-6,8-difluoro-7-hydroxycoumarin) is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The blue excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Blue excitation/emission spectra (371/464 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

The CellTracker™ Blue CMF2HC fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell-impermeant reaction products. CellTracker™ Blue CMF2HC dye is retained in living cells through several generations. The dye is transferred to daughter cells but not adjacent cells in a population. CellTracker™ Blue CMF2HC dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Blue CMF2HC dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.

Click-iT™ Biotin sDIBO Alkyne (Invitrogen™)

Click-iT Biotin sDIBO Alkyne reacts with azides via a copper-free Click chemistry reaction to produce biotin bioconjugates. sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less “sticky” and give lower signal background in biological samples. Copper-free Click bio-conjugation reactions are ideal for surface labeling of live cells and also minimize damage to enzymes and fluorescent proteins like GFP or R-PE. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be now be labeled easily, yielding more soluble bioconjugates with improved biological labeling utility.

• More soluble than DIBO cyclooctynes leading to more soluble conjugates
• Minimal background potential in cells and tissues compared to original DIBO cycloctynes

Learn more about avidin-biotin detection ›

CellTracker™ Deep Red Dye (Invitrogen™)

CellTracker™ Deep Red is a fluorescent dye well suited for monitoring cell movement or location. After loading the cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The deep red excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Red excitation/emission spectra (630/650 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

The CellTracker™ Deep Red fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell-impermeant reaction products. CellTracker™ Deep Red dye is retained in living cells through several generations. The dye is transferred to daughter cells but not adjacent cells in a population. CellTracker™ Deep Red dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Deep Red dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.

Influx™ Pinocytic Cell-Loading Reagent (Invitrogen™)

Influx pinocytic cell-loading reagent provides a convenient, rapid and simple procedure for loading water-soluble materials into live cells. With the Influx reagent, polar compounds can be introduced into many cells simultaneously without significantly altering normal cell function.

Qtracker™ 655 Vascular Labels (Invitrogen™)

Qtracker® non-targeted quantum dots are designed to be injected into the tail vein of mice for the study of vascular structure using small animal in vivo imaging (SAIVI) techniques. These nanocrystals exhibit intense fluorescence with red-shifted emission for increased tissue penetration, and have a PEG surface coating specially developed to minimize nonspecific interactions and reduce immune response by the tissue. Because the PEG surface coating does not contain reactive functional groups, the Qtracker® non-targeted quantum dots are retained in circulation longer and can be imaged for up to 3 hours with a single injection or for longer periods of time with additional injections.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 655 label has Ex/Em (405-615/655) nm
Designed for small animal in vivo imaging
Introduced via tail vein injection, can be imaged for up to 3 hours after injection
Available in four colors—565 nm, 655 nm, 705 nm, or 800 nm emission

Read more about SAIVI and about applications for Qdot® nanocrystals.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

CellVue™ Plum Cell Labeling Kit (Invitrogen™)

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. The Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Plum is a far-red fluorescent cell labeling reagent. It is optimally excited at 652 nm and has a peak emission of 671 nm, however, it can be detected equally in filter sets designed for APC, Alexa Fluor™ 700, and APC-eFluor™ 780 or similar fluorochromes. Therefore, it is not recommended to use antibodies conjugated to these fluorochromes when using CellVue™ Plum.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers (Invitrogen™)

Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).

Click-iT™ Alexa Fluor™ 488 sDIBO Alkyne (Invitrogen™)

Click-iT Alexa Fluor 488 sDIBO Alkyne reacts with azides via a copper-free Click chemistry reaction to produce fluorescent Alexa Fluor 488 bioconjugates. sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less “sticky” and give lower signal background in biological samples. Copper-free Click bio-conjugation reactions are ideal for surface labeling of live cells and also minimize damage to enzymes and fluorescent proteins like GFP or R-PE. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be now be labeled easily, yielding more soluble bioconjugates with improved biological labeling utility.

• More soluble than DIBO cyclooctynes leading to more soluble conjugates
• Minimal background potential in cells and tissues compared to original DIBO cycloctynes

Learn more about Alexa Fluor dyes ›

Qtracker™ 800 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 800 Cell Labeling Kit use a custom targeting peptide to deliver near-infrared-fluorescent Qdot® 800 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 800 label has Ex/Em (405-760/800) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

CellTracker™ Orange CMRA Dye (Invitrogen™)

CellTracker™ Orange CMRA is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The orange excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Orange excitation/emission spectra (548/576 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker™ Orange CMRA fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into a cell-impermeant, fluorescent dye. CellTracker™ Orange CMRA dye is retained in living cells through several generations. The dye is transferred to daughter but not adjacent cells in a population. CellTracker™ Orange CMRA dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Orange CMRA dye are well separated from GFP (green fluorescent protein) spectra allowing for multiplexing.

Qtracker™ 625 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 625 Cell Labeling Kit use a custom targeting peptide to deliver orange/red-fluorescent Qdot® 625 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 625 label has Ex/Em (405-585/625) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

Qtracker™ 705 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 705 Cell Labeling Kit use a custom targeting peptide to deliver near-infrared-fluorescent Qdot® 705 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 705 label has Ex/Em (405-665/705) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

CellTrace™ Calcein Blue, AM - Special Packaging (Invitrogen™)

CellTrace calcein blue AM is a proven short-term, blue-fluorescent tracer for cell labeling. The probe possesses acetoxymethyl (AM) esters that allow its passive diffusion across cell membranes. Upon cleavage of the AM esters by intracellular esterases, the molecule that remains (absorption/emission maxima ~360/449 nm) is relatively polar and is retained by cells for several hours.

View a selection guide for all Nonfixable Viability Dyes for Flow Cytometry.

Vybrant™ CFDA SE Cell Tracer Kit (Invitrogen™)

Vybrant® CFDA SE Cell Tracer Kit is used in fluorescent analyses as a long-term tracer of cells. This method is commonly used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating.

• Superior performance—allows for bright, uniform staining of cells
• Long-term retention—dye is well-retained in cells for several days post stain
• Non-toxic—minimal cytotoxicity for in vivo and in vitro assays
• Simple, robust staining protocol

Superior Fluorescent Staining
Successful tracing of cells requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence. The intense fluorescent staining provided by Vybrant® CFDA SE Cell Tracer Kit enables the visualization of labeled cells for several days post stain, making it an ideal stain for cell tracing studies based on dye dilution.

Long-Term Signal Retention
Vybrant® CFDA SE dye easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye is designed to provide a consistent signal, even after several days in a cell culture environment.

Non-Toxic
Vybrant® CFDA SE dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells. Researchers have used CFDA SE labeling to show that transplantable hematopoietic cells proliferate in vitro in response to stimulation by a growth factor cocktail.

Simple, Robust Staining Protocol
The Vybrant® CFDA SE Cell Tracer Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye, thereby extending the shelf life of the dye not dissolved in solution. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1 µl of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.

Qtracker™ 525 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 525 Cell Labeling Kit use a custom targeting peptide to deliver green-fluorescent Qdot® 525 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 525 label has Ex/Em (405-485/525) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

CellTrace™ Calcein Red-Orange, AM - Special Packaging (Invitrogen™)

CellTrace calcein red-orange AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. Unlike calcein AM (C-1430, C-3099, C-3100), CellTrace calcein red-orange AM is intrinsically fluorescent; thus, an additional wash step may be necessary to minimize background fluorescence from dye that is not taken up by cells. However, CellTrace calcein red-orange (excitation/emission maxima 577/590 nm) is well-retained by live cells that possess intact plasma membranes, and consequently it is a useful cell tracer and indicator of cell viability.

Qtracker™ 655 Cell Labeling Kit (Trial Size) (Invitrogen™)

The Qtracker® 655 Cell Labeling Kit uses a custom targeting peptide to deliver far red-fluorescent Qdot® 655 probe (ex/em 405–615/655 nm) into the cytoplasm of live cells. Once inside the cells, the Qdot® 655 probe provides an intense, stable fluorescence that can be traced through several generations and is not transferred to adjacent cells in a population. This trial-size version offers a convenient introduction to the Qtracker® cell labeling technology at an affordable price.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Features of the Qtracker® Cell Labeling kits include:

• Qtracker® probes allow for continuous illumination, without photobleaching or degradation problems often associated with organic dyes
• Simple to use—add Qtracker® labeling solution to cells in serum-containing media, incubate one hour, then detect and track the cells
• Provide an intense, stable fluorescence that can be traced through several generations
• Excellent tool for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling kits use a custom targeting peptide to deliver Qdot® probes into the cytoplasm of live cells. Since the cytoplasmic delivery mechanism is not mediated by a specific enzyme, no cell-type specificity has been observed. Maximum delivery is typically accomplished in less than 1 hour.

The Qdot® probes are compatible with serum-sensitive cells. The intense fluorescence from the Qdot® probes is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity.

Long-Lasting, Targeted Signal
Use of Qtracker® Cell Labeling kits results in the ability to observe labeled cells with extensive continuous illumination, without photobleaching or degradation problems often associated with organic dyes. Once inside the cells, the Qdot® probes are localized to cytoplasmic vesicles and are inherited by daughter cells for at least six generations. Fluorescence from the Qdot® probes can be detected for up to a week after delivery in some cell lines. The long-term cellular retention of the Qdot® probes results in an ideal tool for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Since the Qtracker® labels do not leak out of intact labeled cells and are not transferred to adjacent cells, the Qtracker® Cell Labeling kits result in a targeted signal.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials used in Qtracker® Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker®-labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

Click-iT™ Amine sDIBO Alkyne (Invitrogen™)

Click-iT Amine sDIBO Alkyne reacts with azides via a copper-free Click chemistry reaction to produce amine bioconjugates. sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less “sticky” and give lower signal background in biological samples. Copper-free Click bio-conjugation reactions are ideal for surface labeling of live cells and also minimize damage to enzymes and fluorescent proteins like GFP or R-PE. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be now be labeled easily, yielding more soluble bioconjugates with improved biological labeling utility.

• More soluble than DIBO cyclooctynes leading to more soluble conjugates
• Minimal background potential in cells and tissues compared to original DIBO cycloctynes

CellTracker™ Violet BMQC Dye (Invitrogen™)

CellTracker™ Violet BMQC is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The violet excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Violet excitation/emission spectra (415/516 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

The CellTracker™ Violet BMQC fluorescent dye has been designed to freely pass through cell membranes into cells, where it is is transformed into cell-impermeant reaction products. CellTracker™ Violet BMQC dye is retained in living cells through several generations. The dye is transferred to daughter cells but not adjacent cells in a population. CellTracker™ Violet BMQC dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Violet BMQC dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.

CellTracker™ Orange CMTMR Dye (Invitrogen™)

CellTracker™ Orange CMTMR (5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethylrhodamine) (mixed isomers) is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the orange excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Orange excitation/emission spectra (541/565 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker™ Orange CMTMR fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CellTracker™ Orange CMTMR dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CellTracker™ Orange CMTMR dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Orange CMTMR dye are well separated from GFP (green fluorescent protein) and spectra allowing for multiplexing.

CellTracker™ Green CMFDA Dye (Invitrogen™)

CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate) is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The green excitation/emission spectra are ideal for multiplexing with red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Green excitation/emission spectra (492/517 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker™ Green CMFDA fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into a cell-impermeant, fluorescent product. CellTracker™ Green fluorescent dyes are retained in living cells through several generations. The dyes are transferred to daughter cells but not adjacent cells in a population. CellTracker™ Green CMFDA dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Green CMFDA dye are well separated from RFP (red fluorescent protein) spectra allowing for multiplexing.

Molecular Probes® Cell Imaging Kit (Invitrogen™)

Fluorescence imaging protocols are sometimes complicated and tedious, with more time spent preparing samples and reagents than acquiring images and data. To help researchers focus more on biology and get results faster, we have developed a set of imaging reagents that emphasize user friendliness and workflow convenience.

The Molecular Probes® Cell Imaging Kit contains the following products:

NucBlue™ Live ReadyProbes™ Reagent (Cat. No. R37605)
NucRed™ Live 647 ReadyProbes™ Reagent (Cat. No. R37106)
ReadyProbes™ Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor™ 594 conjugate (Cat. No. R37117)
Goat Anti-Mouse lgG Secondary Antibody, Alexa Fluor™ 488 conjugate (Cat. No. R37120)
Image-iT™ FX Signal Enhancer ReadyProbes™ Reagent (Cat. No. R37107)

CellTracker™ Blue CMAC Dye (Invitrogen™)

CellTracker™ Blue CMAC (7-amino-4-chloromethylcoumarin) is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the blue excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Blue excitation/emission spectra (353/466 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker™ Blue CMAC fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CellTracker™ Blue CMAC dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CellTracker™ Blue CMAC dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Blue CMAC dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.

Qtracker™ 565 Vascular Labels (Invitrogen™)

Qtracker® non-targeted quantum dots are designed to be injected into the tail vein of mice for the study of vascular structure using small animal in vivo imaging (SAIVI) techniques. These nanocrystals exhibit intense fluorescence with red-shifted emission for increased tissue penetration, and have a PEG surface coating specially developed to minimize nonspecific interactions and reduce immune response by the tissue. Because the PEG surface coating does not contain reactive functional groups, the Qtracker® non-targeted quantum dots are retained in circulation longer and can be imaged for up to 3 hours with a single injection or for longer periods of time with additional injections.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 565 label has Ex/Em (405-525/565) nm
Designed for small animal in vivo imaging
Introduced via tail vein injection, can be imaged for up to 3 hours after injection
Available in four colors—565 nm, 655 nm, 705 nm, or 800 nm emission

Read more about SAIVI and about applications for Qdot® nanocrystals.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

CellVue™ Burgundy Cell Labeling Kit (Invitrogen™)

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. The Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Burgundy is a far-red/near-infrared fluorescent cell labeling reagent. It is optimally excited at 683 nm and has a peak emission of 707 nm. CellVue™ Burgundy can be excited by the red laser line (633 nm), however, it can be detected equally in filter sets designed for Alexa Fluor™ 700 and APC-eFluor™ 780 or similar fluorochromes. Therefore, it is not recommended to use antibodies conjugated to these fluorochromes when using CellVue™ Burgundy. CellVue™ Burgundy can be used in combination with APC.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

OPP (O-propargyl-puromycin) (Invitrogen™)

OPP (O-propargyl-puromycin) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media. When used with Click-iT® Plus OPP Alexa Fluor® Protein Synthesis assays or Alexa Fluor® Picolyl Azide toolkits, OPP provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy, high-content imaging, or flow cytometry.

Probenecid, Water Soluble (Invitrogen™)

Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to poor loading or a high background signal in assays based on retention of the dyes or indicators inside cells. The use of probenecid to block the efflux of intracellular dyes was first described by Di Virgilio et al. (1990), and it has been used with a wide range of anionic dyes and conjugates. The commonly used free acid form of probenecid is difficult to dissolve, requiring 1 M NaOH to get it into solution. Our water-soluble probenecid (P36400) dissolves quickly in assay buffer and eliminates the need to handle caustic NaOH. The water-soluble probenecid is included in the Fluo-4 NW Calcium Assay Kits (F36205, F36206) and is also available separately.

CellTrace™ BODIPY™ TR Methyl Ester (Lipophilic Counterstain For GFP) (Invitrogen™)

The red-fluorescent CellTrace™ BODIPY® TR methyl ester is an excellent counterstain for cells and tissues that are expressing GFP. CellTrace™ BODIPY® TR methyl ester readily permeates cell membranes and selectively stains mitochondria and endomembranous organelles such as endoplasmic reticulum and the Golgi apparatus, but does not appear to localize in the plasma membrane. CellTrace™ BODIPY® TR methyl ester staining is retained after paraformaldehyde fixation.

Click-iT™ Alexa Fluor™ 555 sDIBO Alkyne (Invitrogen™)

Click-iT Alexa Fluor 555 sDIBO Alkyne reacts with azides via a copper-free Click chemistry reaction to produce fluorescent Alexa Fluor 555 bioconjugates. sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less “sticky” and give lower signal background in biological samples. Copper-free Click bio-conjugation reactions are ideal for surface labeling of live cells and also minimize damage to enzymes and fluorescent proteins like GFP or R-PE. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be now be labeled easily, yielding more soluble bioconjugates with improved biological labeling utility.

• More soluble than DIBO cyclooctynes leading to more soluble conjugates
• Minimal background potential in cells and tissues compared to original DIBO cycloctynes

Learn more about Alexa Fluor dyes ›

CellVue™ Jade Cell Labeling Kit (Invitrogen™)

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Jade is a green fluorescent cell labeling reagent. It is optimally excited at 478 nm and has a peak emission of 508 nm. CellVue™ Jade is compatible with most multi-color applications; however, due to similar emission properties it cannot be used in combination with FITC or Alexa Fluor™ 488 reagents.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

Qtracker™ 800 Cell Labeling Kit (Trial Size) (Invitrogen™)

The Qtracker® 800 Cell Labeling Kit uses a custom targeting peptide to deliver near-infrared fluorescent Qdot® 800 probe (ex/em 405–760/800 nm) into the cytoplasm of live cells. Once inside the cells, the Qdot® 800 probe provides an intense, stable fluorescence that can be traced through several generations and is not transferred to adjacent cells in a population. This trial-size version offers a convenient introduction to the Qtracker® cell labeling technology at an affordable price.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Features of the Qtracker® Cell Labeling kits include:

• Qtracker® probes allow for continuous illumination, without photobleaching or degradation problems often associated with organic dyes
• Simple to use—add Qtracker® labeling solution to cells in serum-containing media, incubate one hour, then detect and track the cells
• Provide an intense, stable fluorescence that can be traced through several generations
• Excellent tool for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling kits use a custom targeting peptide to deliver Qdot® probes into the cytoplasm of live cells. Since the cytoplasmic delivery mechanism is not mediated by a specific enzyme, no cell-type specificity has been observed. Maximum delivery is typically accomplished in less than 1 hour.

The Qdot® probes are compatible with serum-sensitive cells. The intense fluorescence from the Qdot® probes is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity.

Long-Lasting, Targeted Signal
Use of Qtracker® Cell Labeling kits results in the ability to observe labeled cells with extensive continuous illumination, without photobleaching or degradation problems often associated with organic dyes. Once inside the cells, the Qdot® probes are localized to cytoplasmic vesicles and are inherited by daughter cells for at least six generations. Fluorescence from the Qdot® probes can be detected for up to a week after delivery in some cell lines. The long-term cellular retention of the Qdot® probes results in an ideal tool for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Since the Qtracker® labels do not leak out of intact labeled cells and are not transferred to adjacent cells, the Qtracker® Cell Labeling kits result in a targeted signal.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials used in Qtracker® Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker®-labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

CellTracker™ Red CMTPX Dye (Invitrogen™)

CellTracker™ Red CMTPX is a fluorescent dye well suited for monitoring cell movement or location. After loading the cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The deep red excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Red excitation/emission spectra (577/602 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

The CellTracker™ Red CMTPX fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell-impermeant reaction products. CellTracker™ Red CMTPX dye is retained in living cells through several generations. The dye is transferred to daughter cells but not adjacent cells in a population. CellTracker™ Red CMTPX dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Red CMTPX dye are well separated from GFP (green fluorescent protein) spectra allowing for multiplexing.

Qtracker™ 705 Cell Labeling Kit (Trial Size) (Invitrogen™)

The Qtracker® 705 Cell Labeling Kit uses a custom targeting peptide to deliver near-infrared fluorescent Qdot® 705 probe (ex/em 405–665/705 nm) into the cytoplasm of live cells. Once inside the cells, the Qdot® 705 probe provides an intense, stable fluorescence that can be traced through several generations and is not transferred to adjacent cells in a population. This trial-size version offers a convenient introduction to the Qtracker® cell labeling technology at an affordable price.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Features of the Qtracker® Cell Labeling kits include:

• Qtracker® probes allow for continuous illumination, without photobleaching or degradation problems often associated with organic dyes
• Simple to use—add Qtracker® labeling solution to cells in serum-containing media, incubate one hour, then detect and track the cells
• Provide an intense, stable fluorescence that can be traced through several generations
• Excellent tool for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling kits use a custom targeting peptide to deliver Qdot® probes into the cytoplasm of live cells. Since the cytoplasmic delivery mechanism is not mediated by a specific enzyme, no cell-type specificity has been observed. Maximum delivery is typically accomplished in less than 1 hour.

The Qdot® probes are compatible with serum-sensitive cells. The intense fluorescence from the Qdot® probes is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity.

Long-Lasting, Targeted Signal
Use of Qtracker® Cell Labeling kits results in the ability to observe labeled cells with extensive continuous illumination, without photobleaching or degradation problems often associated with organic dyes. Once inside the cells, the Qdot® probes are localized to cytoplasmic vesicles and are inherited by daughter cells for at least six generations. Fluorescence from the Qdot® probes can be detected for up to a week after delivery in some cell lines. The long-term cellular retention of the Qdot® probes results in an ideal tool for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Since the Qtracker® labels do not leak out of intact labeled cells and are not transferred to adjacent cells, the Qtracker® Cell Labeling kits result in a targeted signal.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials used in Qtracker® Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker®-labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

CellTrace™ Calcein Violet, AM, for 405 nm excitation - Special Packaging (Invitrogen™)

CellTrace™ calcein violet AM is an optimal dye for distinguishing live cells through the presence of ubiquitous intracellular esterase activity. The enzymatic conversion of the virtually non-fluorescent cell-permeant calcein violet AM to the intensely fluorescent calcein violet (ex/em 400/452 nm) can be easily detected with the violet laser, allowing other laser lines to be used for conventional fluorochromes.

View a selection guide for all Nonfixable Viability Dyes for Flow Cytometry.

Qtracker™ 525 Cell Labeling Kit (Trial Size) (Invitrogen™)

The Qtracker® 525 Cell Labeling Kit uses a custom targeting peptide to deliver green-fluorescent Qdot® 525 probe (ex/em 405–485/525 nm) into the cytoplasm of live cells. Once inside the cells, the Qdot® 525 probe provides an intense, stable fluorescence that can be traced through several generations and is not transferred to adjacent cells in a population. This trial-size version offers a convenient introduction to the Qtracker® cell labeling technology at an affordable price.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Features of the Qtracker® Cell Labeling kits include:

• Qtracker® probes allow for continuous illumination, without photobleaching or degradation problems often associated with organic dyes
• Simple to use—add Qtracker® labeling solution to cells in serum-containing media, incubate one hour, then detect and track the cells
• Provide an intense, stable fluorescence that can be traced through several generations
• Excellent tool for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling kits use a custom targeting peptide to deliver Qdot® probes into the cytoplasm of live cells. Since the cytoplasmic delivery mechanism is not mediated by a specific enzyme, no cell-type specificity has been observed. Maximum delivery is typically accomplished in less than 1 hour.

The Qdot® probes are compatible with serum-sensitive cells. The intense fluorescence from the Qdot® probes is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity.

Long-Lasting, Targeted Signal
Use of Qtracker® Cell Labeling kits results in the ability to observe labeled cells with extensive continuous illumination, without photobleaching or degradation problems often associated with organic dyes. Once inside the cells, the Qdot® probes are localized to cytoplasmic vesicles and are inherited by daughter cells for at least six generations. Fluorescence from the Qdot® probes can be detected for up to a week after delivery in some cell lines. The long-term cellular retention of the Qdot® probes results in an ideal tool for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Since the Qtracker® labels do not leak out of intact labeled cells and are not transferred to adjacent cells, the Qtracker® Cell Labeling kits result in a targeted signal.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials used in Qtracker® Cell Labeling kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker®-labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

CellTrace™ Calcein Green, AM - Special Packaging (Invitrogen™)

CellTrace calcein green AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent CellTrace calcein green AM is converted to a green-fluorescent calcein after intracellular esterases remove the acetoxymethyl (AM) esters. This dye is also available as 1 mg of the solid (C-1430) and resuspended in DMSO (C-3099). For a longer wavelength version of this dye, check out our CellTrace calcein red-orange AM (C-34851).

Qtracker™ 565 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 565 Cell Labeling Kit use a custom targeting peptide to deliver yellow-green-fluorescent Qdot® 565 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 565 label has Ex/Em (405-525/565) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

Qtracker™ 655 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 655 Cell Labeling Kit use a custom targeting peptide to deliver red-fluorescent Qdot® 655 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 655 label has Ex/Em (405-615/655) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

Click-iT™ SDP Ester sDIBO Alkyne (Invitrogen™)

Click-iT SDP Ester sDIBO Alkyne reacts with azides via a copper-free Click chemistry reaction to produce SDP Ester (amine-reactive) bioconjugates. sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less “sticky” and give lower signal background in biological samples. Copper-free Click bio-conjugation reactions are ideal for surface labeling of live cells and also minimize damage to enzymes and fluorescent proteins like GFP or R-PE. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be now be labeled easily, yielding more soluble bioconjugates with improved biological labeling utility.

• More soluble than DIBO cyclooctynes leading to more soluble conjugates
• Minimal background potential in cells and tissues compared to original DIBO cycloctynes

CellTracker™ Green BODIPY™ Dye (Invitrogen™)

CellTracker™ Green BODIPY® (8-chloromethyl-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-S-indacene) is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the green excitation/emission spectra are ideal for multiplexing with red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Green excitation/emission spectra (522/529 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker™ Green BODIPY® fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CellTracker™ Green BODIPY® dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CellTracker™ Green BODIPY® dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Green BODIPY® dye are well separated from RFP (red fluorescent protein) spectra allowing for multiplexing.

Click-iT™ Plus OPP Alexa Fluor™ 647 Protein Synthesis Assay Kit (Invitrogen™)

The Click-iT® Plus OPP Alexa Fluor® 647 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (OPP) is efficiently incorporated into newly translated proteins in complete methionine-containing media and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a fast, highly-specific, and mild click reaction.

Features of the kit include:

• No media change required—works in complete, methionine-containing media, no need to remove cell media
• Multiplex-enabled—Click-iT® Plus technology retains signal from GFP and binding of fluorescent-conjugated phalloidins
• Non-radioactive—an alternative to the traditional 35S-methionine methods
• Works in vivo—published results demonstrate use in vivo for determination of protein translation

The kit contains O-propargyl-puromycin (OPP), which is an alkyne analog of puromycin (also available separately), as well as Alexa Fluor® 647 picolyl azide and all necessary reagents to perform the Click reaction. The OPP is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 647 picolyl azide and the Click reaction reagents leads to a chemoselective ligation, or "click" reaction, between the picolyl azide dye and the OPP alkyne, allowing the modified proteins to be detected by imaged-based analysis.

The click reaction uses bioorthogonal (biologically unique) moieties to fluorescently label proliferating cells, helping to produce low backgrounds and high detection sensitivities. Because of the mild reaction conditions, Click-iT® Plus assays detect protein translation events while enabling preservation of cell morphology, the binding of fluorescently-labeled phalloidin, and the fluorescent signal from GFP.

Unlike 35S-methionine, used in traditional methods, OPP is not an amino acid analog, so it can be added directly to cells in complete media or used to determine protein synthesis in vivo.

The kit contains all of the components needed to label and detect the incorporated OPP in newly translated proteins in samples of adherent cells. The kit includes sufficient reagents for the labeling of 25 18 mm × 18 mm coverslips using 1 mL of reaction buffer per test.

PowerLoad™ Concentrate, 100X (Invitrogen™)

PowerLoad™ is an optimized formulation of nonionic, Pluronic® surfactant polyols for the solubilization of water-insoluble dyes and other materials in physiological media. These surfactants, for instance Pluronic® F-127, have been used to help disperse acetoxymethyl (AM) esters of fluorescent ion indicators such as fluo-4, fura-2, indo-1, fluo-3, and SBFI; they appear to be required for loading of other dyes (e.g. SBFI-AM or PBFI-AM). The use of PowerLoad™ is optional with red shifted calcium indicators and other large molecular weight AM ester dyes, and may also be useful for dispersing other lipophilic probes. The concentration of Pluronic® surfactants in PowerLoad™ is less than 0.2%. PowerLoad™ is effective in combination with water soluble Probenecid (P36400) to aid AM ester dye-loading and retention in cells that actively extrude the de-acetylated form through anion pumps. Together, these reagents allow for maximal loading of dyes with a minimum of effort in both imaging and high throughput screening (HTS) applications. Appropriate controls should be performed to make certain that PowerLoad™ is not altering the membrane properties of the cell.

CellVue™ NIR780 Cell Labeling Kit (Invitrogen™)

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C); Midi CellVue™ Kits are supplied with two vials of dye stock (1 mM in ethanol) and six vials of Diluent C.

CellVue™ NIR780 is a near-infrared fluorescent cell labeling reagent. It can be excited off the red laser line (633 nm) and has a peak emission of 776 nm that can be detected in filter sets designed for APC-eFluor™ 780. CellVue™ NIR780 is compatible with most multi-color applications, however, due to similar emission properties it cannot be used in combination with APC-eFluor™ 780 or APC-Alexa Fluor™ 750 reagents.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

Qtracker™ 800 Vascular Labels (Invitrogen™)

Qtracker® non-targeted quantum dots are designed to be injected into the tail vein of mice for the study of vascular structure using small animal in vivo imaging (SAIVI) techniques. These nanocrystals exhibit intense fluorescence with red-shifted emission for increased tissue penetration, and have a PEG surface coating specially developed to minimize nonspecific interactions and reduce immune response by the tissue. Because the PEG surface coating does not contain reactive functional groups, the Qtracker® non-targeted quantum dots are retained in circulation longer and can be imaged for up to 3 hours with a single injection or for longer periods of time with additional injections.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 800 label has Ex/Em (405-760/800) nm
Designed for small animal in vivo imaging
Introduced via tail vein injection, can be imaged for up to 3 hours after injection
Available in four colors—565 nm, 655 nm, 705 nm, or 800 nm emission

Read more about SAIVI and about applications for Qdot® nanocrystals.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Qtracker™ 585 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 585 Cell Labeling Kit use a custom targeting peptide to deliver yellow-fluorescent Qdot® 585 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 585 label has Ex/Em (405-545/585) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

Click-iT™ Plus OPP Alexa Fluor™ 594 Protein Synthesis Assay Kit (Invitrogen™)

The Click-iT® Plus OPP Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (OPP) is efficiently incorporated into newly translated proteins in complete methionine-containing media and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a fast, highly-specific, and mild click reaction.

Features of the kit include:

• No media change required—works in complete, methionine-containing media, no need to remove cell media
• Multiplex-enabled—Click-iT® Plus technology retains signal from GFP and binding of fluorescent-conjugated phalloidins
• Non-radioactive—an alternative to the traditional 35S-methionine methods
• Works in vivo—published results demonstrate use in vivo for determination of protein translation

The kit contains O-propargyl-puromycin (OPP), which is an alkyne analog of puromycin (also available separately), as well as Alexa Fluor® 594 picolyl azide and all necessary reagents to perform the Click reaction. The OPP is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 picolyl azide and the Click reaction reagents leads to a chemoselective ligation, or "click" reaction, between the picolyl azide dye and the OPP alkyne, allowing the modified proteins to be detected by imaged-based analysis.

The click reaction uses bioorthogonal (biologically unique) moieties to fluorescently label proliferating cells, helping to produce low backgrounds and high detection sensitivities. Because of the mild reaction conditions, Click-iT® Plus assays detect protein translation events while enabling preservation of cell morphology, the binding of fluorescently-labeled phalloidin, and the fluorescent signal from GFP.

Unlike 35S-methionine, used in traditional methods, OPP is not an amino acid analog, so it can be added directly to cells in complete media or used to determine protein synthesis in vivo.

The kit contains all of the components needed to label and detect the incorporated OPP in newly translated proteins in samples of adherent cells. The kit includes sufficient reagents for the labeling of 25 18 mm × 18 mm coverslips using 1 mL of reaction buffer per test.

CellTracker™ Blue CMHC Dye (Invitrogen™)

CellTracker™ Blue CMHC (4-chloromethyl-7-hydroxycoumarin) is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the blue excitation/emission spectra are ideal for multiplexing with green and red fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Blue excitation/emission spectra (372/470 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker™ Blue CMHC fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CellTracker™ Blue CMHC dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CellTracker™ Blue CMHC dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker™ Blue CMHC dye are well separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra allowing for multiplexing.

CellVue™ NIR815 Cell Labeling Kit (Invitrogen™)

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ NIR815 is a near-infrared fluorescent cell labeling reagent. It is optimally excited at 786 nm and has a peak emission of 814 nm. CellVue™ NIR815 has been reported to be useful for short-term in vivo cell tracking studies using a Pearl™ Imager or Odyssey Infrared Imaging System (Li-COR Biosciences, Inc., Lincoln, NE).

Reported Application
Microscopy, Immunocytochemistry, Cell Labeling

Qtracker™ 705 Vascular Labels (Invitrogen™)

Qtracker® non-targeted quantum dots are designed to be injected into the tail vein of mice for the study of vascular structure using small animal in vivo imaging (SAIVI) techniques. These nanocrystals exhibit intense fluorescence with red-shifted emission for increased tissue penetration, and have a PEG surface coating specially developed to minimize nonspecific interactions and reduce immune response by the tissue. Because the PEG surface coating does not contain reactive functional groups, the Qtracker® non-targeted quantum dots are retained in circulation longer and can be imaged for up to 3 hours with a single injection or for longer periods of time with additional injections.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 705 label has Ex/Em (405-665/705) nm
Designed for small animal in vivo imaging
Introduced via tail vein injection, can be imaged for up to 3 hours after injection
Available in four colors—565 nm, 655 nm, 705 nm, or 800 nm emission

Read more about SAIVI and about applications for Qdot® nanocrystals.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Qtracker™ 605 Cell Labeling Kit (Invitrogen™)

The reagents in the Qtracker® 605 Cell Labeling Kit use a custom targeting peptide to deliver orange-fluorescent Qdot® 605 nanocrystals into the cytoplasm of live cells. Qtracker® Cell Labeling Kits are designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals. Once inside the cells, Qtracker® labels provide intense, stable fluorescence that can be traced through several generations, and are not transferred to adjacent cells in a population.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Key Attributes:

Qtracker® 605 label has Ex/Em (405-565/605) nm
Designed for loading cells grown in culture with highly fluorescent Qdot® nanocrystals
Provide intense, stable fluorescence that can be traced through several generations
Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm, or 800 nm emission
Excellent tools for long-term tracking or imaging studies of live cells, including migration, motility, morphology, and other cell function assays

The Qtracker® Cell Labeling Kits use a custom targeting peptide to deliver Qdot® nanocrystals into the cytoplasm of live cells. Cytoplasmic delivery by this mechanism is not mediated by a specific enzyme; therefore, no cell-type specificity has been observed. Delivery is typically accomplished in less than 1 hour.

Qdot® nanocrystals delivered by the Qtracker® Cell Labeling Kits are compatible with serum-sensitive cells; intense fluorescence is maintained in complex cellular environments and under various biological conditions including changes in intracellular pH, temperature, and metabolic activity. Furthermore, autofluorescence commonly observed in cells or tissues can be avoided using Qtracker® 655, 705, or 800 Kits.

Long-Lasting, Targeted Signal
Using Qtracker® Cell Labeling Kits, you can observe labeled cells using extensive continuous illumination, without the photobleaching and degradation problems often associated with organic dyes. Qtracker® labels are distributed in vesicles in the cytoplasm, and are inherited by daughter cells for at least six generations. Fluorescence from the Qtracker® labels can be seen up to a week after delivery in some cell lines. Long-term cellular retention makes Qtracker® Cell Labeling Kits ideal for studying cell motility, migration, differentiation, morphology, and many other cellular function studies. Qtracker® labels do not leak out of intact cells to be taken up by adjacent cells in the population.

Monitor the Signal on Multiple Platforms
Qtracker® reagent-labeled live cells can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal microscopy, fluorescence microplate readers, and high-content imaging systems.

Minimal Impact on Live Cells
The cytotoxicity of the materials use in Qtracker® Cell Labeling Kits has been tested in a variety of cell lines including CHO, HeLa, U-118, 3T3, HUVEC, and Jurkat cells. Labeling with Qtracker® Cell Labeling Kits appears to exert minimal impact on cellular surface marker expression, cell proliferation, cellular enzyme activity, and cell motility.

Useful in a Variety of Cell Tracing Studies
Post-labeling, researchers have demonstrated a wide variety of applications for Qtracker® labeled cells, including cell co-culture and cell assembly into heterotypic assemblies, multilineage differentiation, trans-differentiation versus cell fusion, embryonic and mesenchymal stem cell tracking, and cell migration dynamics.

Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen™)

The Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (OPP) is efficiently incorporated into newly translated proteins in complete methionine-containing media and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a fast, highly-specific, and mild click reaction.

Features of the kit include:

• No media change required—works in complete, methionine-containing media, no need to remove cell media
• Multiplex-enabled—Click-iT® Plus technology retains signal from GFP and binding of fluorescent-conjugated phalloidins
• Non-radioactive—an alternative to the traditional 35S-methionine methods
• Works in vivo—published results demonstrate use in vivo for determination of protein translation

The kit contains O-propargyl-puromycin (OPP), which is an alkyne analog of puromycin (also available separately), as well as Alexa Fluor® 488 picolyl azide and all necessary reagents to perform the Click reaction. The OPP is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 picolyl azide and the Click reaction reagents leads to a chemoselective ligation, or "click" reaction, between the picolyl azide dye and the OPP alkyne, allowing the modified proteins to be detected by imaged-based analysis.

The click reaction uses bioorthogonal (biologically unique) moieties to fluorescently label proliferating cells, helping produce low backgrounds and high detection sensitivities. Because of the mild reaction conditions, Click-iT® Plus assays detect protein translation events while enabling the preservation of cell morphology, the binding of fluorescently-labeled phalloidin, and the fluorescent signal from GFP.

Unlike 35S-methionine, used in traditional methods, OPP is not an amino acid analog, so it can be added directly to cells in complete media or used to determine protein synthesis in vivo.

The kit contains all of the components needed to label and detect the incorporated OPP in newly translated proteins in samples of adherent cells. The kit includes sufficient reagents for the labeling of 25 18 mm × 18 mm coverslips using 1 mL of reaction buffer per test.

CellVue™ Lavender Cell Labeling Kit (Invitrogen™)

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Lavender is a violet fluorescent cell labeling reagent. It is optimally excited at 420 nm and has a peak emission of 461 nm. CellVue™ Lavender is compatible with most multi-color flow cytometric applications; however, due to similar emission properties it cannot be used in combination with eFluor™ 450 or Pacific Blue™ reagents.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling