Shop All DNA⁄RNA Purification Reagents

Lysis Buffer 1 + RNase for Food ID

This convenient packaging of Lysis Buffer 1 + RNase for Food ID is recommended for use with the PrepSEQ Nucleic Acid Extraction Kit (Cat. No. 4480466) to prepare DNA for meat ID and GMO testing from food and feed samples. The protocol using Lysis Buffer 1 + RNase in conjunction with the PrepSEQ Nucleic Acid Extraction Kit can be readily automated on the Thermo Scientific KingFisher mL Food Protection Purification System platform to reduce hands-on time and increase reproducibility of the DNA extraction procedure. The purified DNA can then be used in PCR or other molecular biology techniques, and is well-suited for use with the following food and feed testing kits:

NameCatalog Number
TaqMan GMO Screening Kit4466334
TaqMan Roundup Ready Soya Quantification Kit4466335
TaqMan GMO Maize Quantification Kit4481972
RapidFinder Beef ID KitA24391
RapidFinder Pork ID KitA24392
RapidFinder Equine ID KitA15570
RapidFinder Poultry ID KitA24397
RapidFinder Chicken ID KitA24393
RapidFinder Turkey ID KitA24394
RapidFinder Ruminent ID KitA24396

MagMAX™ Cell-Free DNA Lysis/Binding Buffer (Applied Biosystems™)

This buffer is included in the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). It is made available separately for applications that require more Lysis/Binding Buffer than is provided in the kit.

PureLink™ HiPure BAC Buffer Kit (Invitrogen™)

The PureLink® HiPure BAC Buffer Kit provides additional buffers and reagents for performing BAC (Bacterial Artificial Chromosome) DNA isolation using the PureLink® HiPure Plasmid Kits. The PureLink® HiPure Plasmid Kits utilize a proprietary anion-exchange resin which allows you to purify high-quality BAC DNA from E. coli cultures. BAC DNA requires the use of large bacterial culture volumes to obtain a reasonable DNA yield. The additional buffers and reagents needed for working with larger bacterial cultures are provided in this Buffer Kit. From a 100 ml culture, you can expect to purify ~40 µg of BAC DNA. The PureLink® HiPure BAC Buffer Kit provides reagents sufficient for five BAC Maxipreps, or 25 BAC Midipreps, or 100 BAC Minipreps.

MagMAX™ Cell-Free DNA Wash Solution (Applied Biosystems™)

This solution is included in the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). It is made available separately for applications that require more Wash Solution than is provided in the kit.

MagMAX™ Cell-Free Total Nucleic Acid Isolation Kit (Applied Biosystems™)

The MagMAX Cell-Free Total Nucleic Acid Isolation Kit is designed for enrichment of all nucleic acid species present in cell-free biological samples such as plasma. The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.

The magnetic bead–based purification format allows you to easily process a wide range of sample input volumes (1 mL to 6 mL). When used with the KingFisher Duo Prime or KingFisher Flex Magnetic Particle Processor, 6 to 24 plasma samples of 2-mL input volume can be isolated at once. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX Cell-Free Total Nucleic Acid Isolation Kit include:
• Automation-ready, phenol-free extraction
• Fast procedure allows 6 to 24 samples to be processed in 90 minutes or less when used with the KingFisher Duo Prime or KingFisher Flex Magnetic Particle Processor with a 24 Deep Well Head
• Flexible protocol accommodates sample volume inputs ranging from 1 mL to 6 mL of plasma
• No need for carrier RNA
• Elution volumes ranging from 15 µL to 60 µL
• Compatible with the Oncomine Lung cfTNA Research Assay

Go beyond cell-free DNA and get the total picture
Isolating and analyzing circulating cell-free total nucleic acid (cfTNA) from plasma and other cell-free samples serves as a non-invasive method to detect and monitor targets associated with certain disease states such as cancer.

The first step in attaining this valuable information is efficient nucleic acid isolation that specifically recovers the circulating nucleic acid species that contains information related to the disease state. The importance of cell-free DNA (cfDNA) is becoming established, but it may not tell the whole story. Cell-free RNA (cfRNA) sequences serve as critical biomarkers as well in the form of microRNAs and gene fusions. This inclusion of the cfRNA with the cfDNA provides an opportunity for researchers to expand their studies and advance human health.

The MagMAX Cell-Free Total Nucleic Acid kit is developed by the same Thermo Fisher Scientific scientists that brought you the MagMAX Cell-Free DNA Isolation Kit. The difference is the inclusion of all cfRNA species in the eluate, allowing you to increase the amount of information available from a single cell-free sample. Just like the cfDNA Isolation Kit, the cfTNA kit is designed for both manual and automated isolation of nucleic acid from a wide sample volume input range of 1 to 6 mL. The phenol-free, magnetic-bead format makes the kit fully amenable to automated isolation.

Thanks to the scalable format that allows for various sample inputs, it is possible to vary the number of samples processed with a single kit. For example, enough reagents are provided to process 50 samples of 2 mL plasma each. Alternatively, with the same kit it is possible to process 25 samples of 4 mL plasma each. Higher and lower input volumes are possible and the number of samples that can be processed will vary accordingly. This unique product attribute allows you to tailor the product to your specific needs.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with protein-rich, large-volume samples such as plasma that are commonly used in circulating cell-free nucleic acid studies.

MagMAX™ Cell-Free DNA Elution Solution (Applied Biosystems™)

This solution is included in the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). It is made available separately for applications that require more Elution Solution than is provided in the kit.

Extra MagMAX FFPE DNA/RNA Ultra Reagents (Applied Biosystems™)

This package includes supplemental reagents for use with the MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881). These additional reagents consist of protease digestion buffer, binding solution, and DNA wash buffer and are specifically for use when processing >40 um sections. See the User Guide for details.

MagMAX™ CORE Glass Microbeads (Applied Biosystems™)

MagMAX CORE Glass Microbeads are offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module. The beads aid in the homogenization of completely dessicated feces such as environmental samples or fecal pellets.

For a kit that contains these microbeads as well as the MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module, please see Cat. No. A37488.

Plant DNAzol™ Reagent (Invitrogen™)

Plant DNAzol® Reagent is a ready-to-use organic reagent formulated for the isolation of high quality genomic DNA from a variety of plant samples.

• Permits use on broad spectrum plant material
• Rapid isolation of genomic DNA

RNA Hydrolysis for Efficient Isolation of Genomic DNA from a Variety of Sample Sources
Plant DNAzol® Reagent can be used for the efficient isolation of genomic DNA from a variety of plant tissues such as leaf, seed stem, root, and callus. The Plant DNAzol® procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate.

Isolate Genomic Plant DNA in About an Hour
In the Plant DNAzol® procedure, plant samples are pulverized in liquid nitrogen or homogenized, and genomic DNA is extracted from the homogenate with the Plant DNAzol® Reagent. Following extraction, plant debris is removed by centrifugation and DNA is precipitated from the supernatant with ethanol, and solubilized with TE buffer (pH 8.0). The entire procedure can be completed in approximately 60-70 minutes.

Comparable Yield in Less Time and with Fewer Reagents
Unlike traditional methods for plant DNA extraction (e.g., CTAB), Plant DNAzol® is quicker, does not require RNase A to remove RNA from genomic DNA preparations, and in the majority of tissues, does not require phenol. Furthermore, use of Plant DNAzol® generates yields from plant tissue that are comparable with the traditional CTAB method.

Isolated DNA can be used for a Number of Downstream Applications:
Amplified Fragment Length Polymorphism (AFLP)
Restriction Fragment Length Polymorphism (RFLP)
Southern Blotting
Sequencing
PCR
Cloning

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagMAX™ Viral/Pathogen Ultra Enzyme Mix (Applied Biosystems™)

This enzyme mix is included in the MagMAX Viral/Pathogen Nucleic Acid Ultra Isolation Kit (Cat. No. A42356). It is made available separately for applications that require more enzyme mix than is provided in the kit.

PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit (Applied Biosystems™)

The PrepSEQ® 1-2-3 Nucleic Acid Extraction Kit uses magnetic particle-based separation
technology to extract DNA from Mycoplasma cells or Mouse Minute Virus
(MMV) isolated from a variety of starting material, such as infected cell cultures
or Mycoplasma liquid cultures.

Using this kit, you prepare lysates of samples before you extract the target DNA.
For samples that contain Mycoplasma, the lysates contain Mycoplasma DNA
from the free organisms in the culture media and from organisms released from
the infected host cells.

Agarase (0.5 U/µL) (Thermo Scientific™)

Thermo Scientific Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides. Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amplification, cloning, sequencing, etc.

MagMAX™ FFPE DNA/RNA Ultra Kit (Applied Biosystems™)

The MagMAX™ FFPE DNA/RNA Ultra Kit is designed for sequential isolation of DNA and RNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample. The DNA and RNA are recovered in separate eluates, and both are compatible with a broad range of applications, including real-time PCR and next-generation sequencing. The isolation of RNA and DNA from the same FFPE sample makes this kit an ideal method of sample preparation for Oncomine™ Comprehensive and Oncomine™ Focus assays.

Features of the MagMAX FFPE DNA/RNA Ultra Kit include:
• Flexible design that permits both manual and automated isolation of RNA and DNA
• Minimal requirement of 5 µm section or curl input
• Capable of processing 40 µm FFPE sections or curls; greater than 40 µm sections can be processed with an alternative protocol
• Compatible with targeted RNA and DNA sequencing panels
• Contains Dynabeads™ MyOne™ Silane for consistent isolation of RNA, microRNA, and DNA
• Alternative protocols for the isolation of RNA or DNA only

Unlocking the information contained in FFPE samples is essential to cancer research. Common nucleic acid isolation methods require you to choose which nucleic acid to recover from a given sample, with loss of the other nucleic acid (and any information that may have resulted from its analysis). Alternatively, the DNA and RNA are co-purified, or the sample is split in half so that each can be purified.

More information from less sample input
The MagMAX FFPE DNA/RNA Ultra Kit protocol addresses these issues by first isolating DNA from the FFPE sample and then recovering the RNA from the DNA-depleted supernatant. The nucleic acids are prepared as separate eluates and are then ready for downstream analysis. This allows for a fuller analysis of precious samples, including investigation of important biomarkers, such as hotspot mutations, CNVs, gene fusions, and indels.

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher™ Duo Prime or KingFisher™ Flex processor.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous FFPE tissue samples that that are not fully digested during the proteinase K treatment.

Optimized bead surface chemistry
The Dynabeads MyOne Silane utilized in the MagMAX FFPE DNA/RNA Ultra Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery of both RNA and DNA targets found in FFPE samples. Once captured, both of these nucleic acids are then eluted separately in volumes of 50 µL and are ready for downstream applications.

PrepMan™ Ultra Sample Preparation Reagent (Applied Biosystems™)

The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step.

PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous fungi, both from a plate or from liquid culture, mammalian tissue smears, human cells (buccal swab), and mammalian whole blood. The resulting template has been used amplified in allelic discrimination assays, genetically modified organism (GMO) detection and quantification, food pathogen detection, microbial identification, and bacterial strain typing.

• Simple, rapid protocol reduces time to results.
• Unique formulation inactivates PCR inhibitors, yielding consistent results.
• PCR-ready template can be prepared from a wide range of sample types.
• Affordable method with a low price per preparation.
• Homogeneous solution with minimal sample-handling requirements.
• Microcentrifuge tube-based format means no additional equipment is needed for template preparation.

PrepMan® Ultra reagent is a novel formulation and is a homogeneous solution that does not contain Chelex® or any other type of resin or matrix.

This product is a replacement⁄improvement of PrepMan® Sample Preparation Reagent, and should be recommended to current and potential PrepMan® Reagent users.

Used With
3500/3500xL Genetic Analyzer Capillary Array (50 cm), 3130xL/3100 Genetic Analyzer Capillary Array (36 cm), 96-Well Plate Septa, AmpliTaq Gold® PCR Master Mix, BigDye® Terminator v1.1 Cycle Sequencing Kit, POP-6™ Polymer for 3100⁄3100-Avant™ Genetic Analyzers, PrepMan® Ultra Sample Preparation Reagent, TaqMan® GMO Maize 35S Detection Kit, TaqMan® GMO Soy 35S Detection Kit.

MagMAX™ CORE Mechanical Lysis Module (Applied Biosystems™)

The Applied Biosystems MagMAX Core sample preparation system is a universal system designed to meet current and future veterinary testing needs. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing. The MagMAX CORE Mechanical Lysis Module is offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of hard-to-lyse organisms like Mycobacterium avium paratuberculosis (MAP) and bovine tuberculosis in feces, milk, and environmental samples.

For a kit that contains this Mechanical Lysis Module as well as the MagMAX CORE Nucleic Acid Purification Kit, please see Cat. No. A37487.

Features include:
Convenience—a complete solution using the same CORE chemistry
Confidence—higher-quality nucleic acids from difficult-to-lyse samples, including both small (0.3 g) and large (2–5 g) volume feces samples
Flexible—purchase only what you need to lyse MAP and other hard-to-lyse organisms

The MagMAX CORE kit and the MagMAX Core Mechanical Lysis Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.