Shop All DNA⁄RNA Purification Reagents

MagMAX CORE Nucleic Acid Purification Kit + Mechanical Lysis Module Applied Biosystems™

This MagMAX CORE Mechanical Lysis Module Bundle includes a MagMAX CORE Mechanical Lysis Module and MagMAX CORE Nucleic Acid Purification Kit. The Mechanical Lysis Module is also available separately.

The Applied Biosystems MagMAX CORE sample preparation solution is a universal sample preparation system designed to meet current and future veterinary testing needs. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing. The MagMAX CORE Mechanical Lysis Module is offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of hard-to-lyse organisms like Mycobacterium avium paratuberculosis (MAP) and bovine tuberculosis in feces, milk, and environmental samples.

Features include:
Convenience—a complete solution using the same CORE chemistry
Confidence—higher-quality nucleic acids from difficult-to-lyse samples, including both small (0.3 g) and large (2–5 g) volume feces samples
Flexible—purchase only what you need to lyse MAP and other hard-to-lyse organisms

The MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.

MagMAX™ Microbiome Bead Tubes Applied Biosystems™

These bead tubes are included in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. No. A42358). They are made available separately for applications that require more tubes than are provided in the kit.

Extra MagMAX FFPE DNA/RNA Ultra Reagents Applied Biosystems™

This package includes supplemental reagents for use with the MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881). These additional reagents consist of protease digestion buffer, binding solution, and DNA wash buffer and are specifically for use when processing >40 um sections. See the User Guide for details.

MagMAX™ Cell and Tissue DNA Extraction Buffer Applied Biosystems™

MagMAX DNA Cell and Tissue Extraction Buffer, a component of the MagMAX DNA Multi-Sample Ultra 2.0 Kit with Cell and Tissue Extraction Buffer, is offered separately here for those kit users who need additional buffer.

MagMAX™ CORE Glass Microbeads Applied Biosystems™

MagMAX CORE Glass Microbeads are offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module. The beads aid in the homogenization of completely dessicated feces such as environmental samples or fecal pellets.

For a kit that contains these microbeads as well as the MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module, please see Cat. No. A37488.

TRIzol™ Reagent Invitrogen™

TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

Key features of TRIzol® Reagent include:

• Permits the isolation of RNA, DNA, and protein from the same sample
• Offers superior lysis capability, even with difficult sample types
• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria

Reliably purify RNA from multiple sample volumes and sources
TRIzol® Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin. TRIzol® Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol® Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® Reagent is free of protein and DNA contamination.

Formulated for isolation of multiple molecular targets
TRIzol® Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

PureLink™ Plant RNA Reagent Invitrogen™

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds). Features of the Plant RNA Reagent:

• Isolate high-quality RNA from difficult plant materials
• Use for small-scale and large-scale RNA isolations

Improved quality and purity of plant RNA
Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol® Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A260/A280 and gel analysis) from a variety of plant material sources.

Works for milligram to gram quantities of starting material
Plant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5 g plant tissue) total RNA isolation. The amount supplied (100 mL) is sufficient for 200 reactions using 100 mg of tissue, or 4 reactions using 5 g of tissue.

Isolated RNA is ideal for a variety of applications and products:

cDNA library construction kits
Real-Time PCR (qPCR)
Northern blotting kits & reagents
Poly(A) selection kit
RNase control reagents & assays

Lysis Buffer 1 + RNase for Food ID

This convenient packaging of Lysis Buffer 1 + RNase for Food ID is recommended for use with the PrepSEQ Nucleic Acid Extraction Kit (Cat. No. 4480466) to prepare DNA for meat ID and GMO testing from food and feed samples. The protocol using Lysis Buffer 1 + RNase in conjunction with the PrepSEQ Nucleic Acid Extraction Kit can be readily automated on the Thermo Scientific KingFisher mL Food Protection Purification System platform to reduce hands-on time and increase reproducibility of the DNA extraction procedure. The purified DNA can then be used in PCR or other molecular biology techniques, and is well-suited for use with the following food and feed testing kits:

NameCatalog Number
TaqMan GMO Screening Kit4466334
TaqMan Roundup Ready Soya Quantification Kit4466335
TaqMan GMO Maize Quantification Kit4481972
RapidFinder Beef ID KitA24391
RapidFinder Pork ID KitA24392
RapidFinder Equine ID KitA15570
RapidFinder Poultry ID KitA24397
RapidFinder Chicken ID KitA24393
RapidFinder Turkey ID KitA24394
RapidFinder Ruminent ID KitA24396

PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit Applied Biosystems™

The PrepSEQ® 1-2-3 Nucleic Acid Extraction Kit uses magnetic particle-based separation
technology to extract DNA from Mycoplasma cells or Mouse Minute Virus
(MMV) isolated from a variety of starting material, such as infected cell cultures
or Mycoplasma liquid cultures.

Using this kit, you prepare lysates of samples before you extract the target DNA.
For samples that contain Mycoplasma, the lysates contain Mycoplasma DNA
from the free organisms in the culture media and from organisms released from
the infected host cells.

PrepMan™ Ultra Sample Preparation Reagent Applied Biosystems™

The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step.

PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous fungi, both from a plate or from liquid culture, mammalian tissue smears, human cells (buccal swab), and mammalian whole blood. The resulting template has been used amplified in allelic discrimination assays, genetically modified organism (GMO) detection and quantification, food pathogen detection, microbial identification, and bacterial strain typing.

• Simple, rapid protocol reduces time to results.
• Unique formulation inactivates PCR inhibitors, yielding consistent results.
• PCR-ready template can be prepared from a wide range of sample types.
• Affordable method with a low price per preparation.
• Homogeneous solution with minimal sample-handling requirements.
• Microcentrifuge tube-based format means no additional equipment is needed for template preparation.

PrepMan® Ultra reagent is a novel formulation and is a homogeneous solution that does not contain Chelex® or any other type of resin or matrix.

This product is a replacement⁄improvement of PrepMan® Sample Preparation Reagent, and should be recommended to current and potential PrepMan® Reagent users.

Used With
3500/3500xL Genetic Analyzer Capillary Array (50 cm), 3130xL/3100 Genetic Analyzer Capillary Array (36 cm), 96-Well Plate Septa, AmpliTaq Gold® PCR Master Mix, BigDye® Terminator v1.1 Cycle Sequencing Kit, POP-6™ Polymer for 3100⁄3100-Avant™ Genetic Analyzers, PrepMan® Ultra Sample Preparation Reagent, TaqMan® GMO Maize 35S Detection Kit, TaqMan® GMO Soy 35S Detection Kit.

MagMAX™ DNA Ultra 2.0 with Cell and Tissue Extraction Buffer Applied Biosystems™

The MagMAX DNA Multi-Sample Ultra 2.0 Kit is an automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from 50 µL to 2 mL of whole blood, saliva, buffy coat, and buccal swabs. The resulting purified gDNA is ideal for a variety of downstream molecular biology applications such as qPCR, next-generation sequencing, and microarray analysis. One kit is sufficient to process 200 50–200 µL samples or 100 200–400 µL samples.

This version of the MagMAX DNA Multi-Sample Ultra 2.0 Kit contains an additional buffer that allows you to extract DNA from tissues and cells as well as blood, saliva, buffy coat, and buccal swabs.

Key features of the MagMAX DNA Multi-Sample Ultra 2.0 Kit:
• Process 6–96 samples in 45 minutes with 5 minutes hands-on time
• Isolate gDNA from 50 µL–2 mL of sample using a simple protocol in SBS microtiter plates
• Skip sample and buffer volume normalization steps for hassle-free processing
• Download validated BindIt software automation scripts for KingFisher Duo Prime and Flex purification systems
• Automation-friendly stand-alone MagMAX DNA Multi-Sample Ultra 2.0 reagent bottle options available

Isolate genomic DNA from multiple sample types with a single sample prep kit
The MagMAX DNA Multi-Sample Ultra 2.0 Kit isolates gDNA from 50 µL–2 mL of whole blood (figure below), fresh and stabilized saliva (figure below), and EDTA/Streck cfDNA tube buffy coat (figure below) samples. Additionally, the same kit can be used to isolate gDNA from buccal swabs and other biological sample types. The MagMAX DNA Multi-Sample Ultra 2.0 Kit maximizes gDNA yields by treating samples with proteinase K (PK) and PK-enhancer followed by an innovative lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on the high-binding capacity Ultra 2.0 gDNA magnetic binding beads. The Ultra 2.0 Wash I solution removes proteins, detergents, and other impurities from the bound nucleic acid. The gDNA is then released from the beads using a low-TE elution solution.

Fast and hassle-free MagMAX magnetic bead-based gDNA workflows
The MagMAX DNA Multi-Sample Ultra 2.0 Kit combines multiple laborious sample processing steps together into a single step to minimize the time and effort traditionally required by other methods without compromising yield and purity. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep. Simply match your sample volumes to one of three standard input workflows (50 µL to 400 µL, up to 1 mL, up to 2 mL) and follow the work instructions for either manual or semi-automated sample preparation.

Made for KingFisher purification systems
The MagMAX DNA Multi-Sample Ultra 2.0 Kit is optimized for use on the moderate-throughput KingFisher Duo Prime (Cat. No. 5400110) and the higher-throughput KingFisher Flex (Cat. No. 5400630) purification systems. Users can process up to 400 µL of sample volume in high-throughput SBS 96-well deep-well microtiter plates using the Flex system. For smaller sample batches, users can process 12 samples using the Duo Prime system. Larger sample input volumes (1–2 mL), which are more challenging to process, are purified in SBS 24-well deep-well microtiter plates for either 24 samples on the Flex or 6 samples on the Duo Prime system. KingFisher instrument BindIt software scripts for all sample types and both Flex and Duo Prime systems can be downloaded below and installed onto the KingFisher instruments.

Working with higher throughput sample numbers?
The same MagMAX DNA Multi-Sample Ultra 2.0 Kit reagents are available as automation-friendly stand-alone reagents. MagMAX DNA Multi-Sample Ultra 2.0 stand-alone reagents are individually bottled in larger reagent volumes to make your high-thoughput workflows easier to handle. When purchased together as a sets of 6 stand-alone reagents, users will have sufficient volume of MagMAX DNA Multi-Sample Ultra 2.0 reagents to process 2000 50-200 uL samples or 1000 200-400 ul samples.

MagMAX DNA Ultra Multi-Sample 2.0 Proteinase K (45 mL)
MagMAX DNA Ultra Multi-Sample 2.0 Binding Beads (45 mL)
MagMAX DNA Ultra Multi-Sample 2.0 Wash I Solution (1.1 L)
MagMAX DNA Ultra Multi-Sample 2.0 Lysis/Binding Solution (450 mL)
MagMAX DNA Ultra Multi-Sample 2.0 Elution Solution (120 mL)
MagMAX DNA Ultra Multi-Sample 2.0 Enhancer Solution (45 mL)

TRIzol™ Max™ Bacterial RNA Isolation Kit Invitrogen™

The TRIzol® Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol® Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.

Key Features of the TRIzol® Max™ Bacterial RNA Isolation Kit:

• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps

Two Products in One Kit
The TRIzol® Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol® Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol® Reagent generates a high-quality, minimally degraded RNA product.

Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol® Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System Invitrogen™

This system includes both TRIzol™ LS Reagent and Invitrogen™ Phasemaker™ Tubes.

TRIzol LS Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from a variety of liquid biological samples such as serum and virus preparations.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA. This TRIzol LS Reagent and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate approximately 0.25 mL of sample.

MagMAX™ FFPE DNA/RNA Ultra Kit Applied Biosystems™

The MagMAX™ FFPE DNA/RNA Ultra Kit is designed for sequential isolation of DNA and RNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample. The DNA and RNA are recovered in separate eluates, and both are compatible with a broad range of applications, including real-time PCR and next-generation sequencing. The isolation of RNA and DNA from the same FFPE sample makes this kit an ideal method of sample preparation for Oncomine™ Comprehensive and Oncomine™ Focus assays.

Features of the MagMAX FFPE DNA/RNA Ultra Kit include:
• Flexible design that permits both manual and automated isolation of RNA and DNA
• Minimal requirement of 5 µm section or curl input
• Capable of processing 40 µm FFPE sections or curls; greater than 40 µm sections can be processed with an alternative protocol
• Compatible with targeted RNA and DNA sequencing panels
• Contains Dynabeads™ MyOne™ Silane for consistent isolation of RNA, microRNA, and DNA
• Alternative protocols for the isolation of RNA or DNA only

Unlocking the information contained in FFPE samples is essential to cancer research. Common nucleic acid isolation methods require you to choose which nucleic acid to recover from a given sample, with loss of the other nucleic acid (and any information that may have resulted from its analysis). Alternatively, the DNA and RNA are co-purified, or the sample is split in half so that each can be purified.

More information from less sample input
The MagMAX FFPE DNA/RNA Ultra Kit protocol addresses these issues by first isolating DNA from the FFPE sample and then recovering the RNA from the DNA-depleted supernatant. The nucleic acids are prepared as separate eluates and are then ready for downstream analysis. This allows for a fuller analysis of precious samples, including investigation of important biomarkers, such as hotspot mutations, CNVs, gene fusions, and indels.

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher™ Duo Prime or KingFisher™ Flex processor.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous FFPE tissue samples that that are not fully digested during the proteinase K treatment.

Optimized bead surface chemistry
The Dynabeads MyOne Silane utilized in the MagMAX FFPE DNA/RNA Ultra Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery of both RNA and DNA targets found in FFPE samples. Once captured, both of these nucleic acids are then eluted separately in volumes of 50 µL and are ready for downstream applications.

TRIzol™ Reagent and Phasemaker™ Tubes Complete System Invitrogen™

This system includes both TRIzol™ Reagent and Invitrogen™ Phasemaker™ Tubes.

TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from a variety of biological samples. It offers superior lysis capability, even with difficult sample types.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA. This TRIzol Reagent and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.
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