Shop All DNA⁄RNA Purification Reagents

MagMAX™ Microbiome Lysis Solution (Applied Biosystems™)

This lysis buffer is included in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42357, and A42358). It is made available separately for applications that require more lysis solution than is provided in the kit.

Agarase (0.5 U/µL) (Thermo Scientific™)

Thermo Scientific Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides. Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amplification, cloning, sequencing, etc.

MagMAX™ Viral/Pathogen Binding Beads (Applied Biosystems™)

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). They are made available separately for applications that require more binding beads than are provided in the kit.

MagMAX™ Viral/Pathogen Proteinase K (Applied Biosystems™)

This Proteinase K is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more Proteinase K than is provided in the kit.

MagMAX™ Viral/Pathogen Elution Buffer (Applied Biosystems™)

This elution buffer is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more elution buffer than is provided in the kit.

PrepSEQ™ Residual DNA Sample Preparation Kit (Applied Biosystems™)

The Applied Biosystems® PrepSEQ® Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures of proteins, buffers, and salts which are typical of samples that need to be quantitated for residual host cell line DNA. Quantitative and consistent recovery of DNA can be obtained from challenging matrices, including samples that have high protein concentration, low pH, or high salt

MagMAX™ Viral/Pathogen Ultra Enzyme Mix (Applied Biosystems™)

This enzyme mix is included in the MagMAX Viral/Pathogen Nucleic Acid Ultra Isolation Kit (Cat. No. A42356). It is made available separately for applications that require more enzyme mix than is provided in the kit.

TRIzol™ LS Reagent (Invitrogen™)

TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key features of TRIzol® LS Reagent:

• Formulated for use with liquid samples such as serum and virus preparations
• Facilitates recovery of RNA, DNA, and protein from a single liquid sample
• Offers excellent lysis capability, even with difficult biological fluids

Reliably purify RNA from multiple sample sources
TRIzol® LS Reagent is designed for processing a variety of liquid samples of up to 0.25 mL in volume. TRIzol® LS Reagent differs from the standard TRIzol® Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRIzol® Reagent, the integrity of resulting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRIzol® LS Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® LS Reagent is free of protein and DNA contamination.

Formulated to serve multiple isolations
TRIzol® LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

Purified products are ideal for use with a variety of applications
Isolated RNA can be used in real-time quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecular cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein can be used for western blots, recovery of some enzymatic activity, and some immunoprecipitation

TRIzol™ Max™ Bacterial RNA Isolation Kit (Invitrogen™)

The TRIzol® Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol® Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.

Key Features of the TRIzol® Max™ Bacterial RNA Isolation Kit:

• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps

Two Products in One Kit
The TRIzol® Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol® Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol® Reagent generates a high-quality, minimally degraded RNA product.

Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol® Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagMAX™ CORE Mechanical Lysis Module (Applied Biosystems™)

The Applied Biosystems MagMAX Core sample preparation system is a universal system designed to meet current and future veterinary testing needs. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing. The MagMAX CORE Mechanical Lysis Module is offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of hard-to-lyse organisms like Mycobacterium avium paratuberculosis (MAP) and bovine tuberculosis in feces, milk, and environmental samples.

For a kit that contains this Mechanical Lysis Module as well as the MagMAX CORE Nucleic Acid Purification Kit, please see Cat. No. A37487.

Features include:
Convenience—a complete solution using the same CORE chemistry
Confidence—higher-quality nucleic acids from difficult-to-lyse samples, including both small (0.3 g) and large (2–5 g) volume feces samples
Flexible—purchase only what you need to lyse MAP and other hard-to-lyse organisms

The MagMAX CORE kit and the MagMAX Core Mechanical Lysis Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.

MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes (Applied Biosystems™)

Stool and soil are often the sample types of choice for scientists in the microbiome field. However, these samples also happen to be some of the most challenging to extract RNA and DNA from, until now. The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil. The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing.

Features of the MagMAX Microbiome Nucleic Acid Isolation Kit include:
• Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems
• Fast procedure allows for 96 samples to be processed in ~60 minutes
• Compatible with stool, soil, and swabs contained in transport medium
• Mechanical disruption via bead beating in 96-well plate or individual tubes
• Elution volumes ranging from 50 to 200 µL

Maximize sample input and nucleic acid recovery in minimal time
Up to 100 mg of stool can be processed per well in a KingFisher 96-deep well plate. When the extraction process is performed using a KingFisher Flex system, 96 samples can processed in ~60 minutes; when using a KingFisher Duo Prime system 12 samples can be processed in ~50 minutes. When the protocol is completed, pure RNA and DNA is concentrated in 100 µL of elution buffer.

Two bead beating formats available
High-throughput processing of difficult samples such as stool is possible with 96-deep well plates containing zirconia beads combined with optimized MagMAX Microbiome reagents (Cat. No. A42357). If processing 96 samples at a time is not needed then the same beads are available in tube format for lower-throughput processing (this page). Once the bead beating step is complete, the lysate can then be transferred into a KingFisher 96-deep well plate and processed on a KingFisher Flex or KingFisher Duo Prime system.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with stool samples that are commonly used for microbiome research.

The Dynabeads MyOne Silane contained in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery RNA and DNA. Once captured, these nucleic acids can then be eluted in of 50-100 µL of elution buffer for use in downstream applications.

TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System (Invitrogen™)

This system includes both TRIzol™ LS Reagent and Invitrogen™ Phasemaker™ Tubes.

TRIzol LS Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from a variety of liquid biological samples such as serum and virus preparations.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA. This TRIzol LS Reagent and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate approximately 0.25 mL of sample.

TRIzol™ Reagent (Invitrogen™)

TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

Key features of TRIzol® Reagent include:

• Permits the isolation of RNA, DNA, and protein from the same sample
• Offers superior lysis capability, even with difficult sample types
• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria

Reliably purify RNA from multiple sample volumes and sources
TRIzol® Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin. TRIzol® Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol® Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® Reagent is free of protein and DNA contamination.

Formulated for isolation of multiple molecular targets
TRIzol® Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

TRI Reagent™ Solution (Invitrogen™)

Tri Reagent® solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. Key features:

Simple—a single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein
Productive—obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods
Versatile—works with many sample sources, including human, plant, yeast, bacterial and viral samples
Flexible—easily scalable depending on starting material and desired yield

Using TRI Reagent® solution
TRI Reagent® starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability.

How it works
TRI Reagent® combines phenol and guanidine thiocyanate in a monophase solution to facilitate the immediate inhibition of RNase activity. Biological samples are homogenized or lysed in TRI Reagent®; the subsequent addition of bromochloropropane or chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The RNA can then be precipitated from the aqueous phase with the addition of isopropanol. The entire protocol can be completed in approximately 1 hour.

Applications
The isolated RNA is suitable for any downstream application, including RT-PCR, amplification for array analysis, hybridization assays, or in vitro translation. PCR, Southern blotting, and cloning can be performed using DNA isolated with TRI Reagent®. Isolated proteins can be used in SDS-PAGE and western blotting.

Tri Reagent® solution is incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA isolation kits.

MagMAX™ CORE Lysis Solution (Applied Biosystems™)

This MagMAX CORE Lysis Solution is made available as a supplement to that included in the MagMAX CORE Nucleic Acid Purification Kit. This proprietary buffer solution is used to break open cells in preparation for downstream molecular detection applications such as real time PCR, RT-PCR, and sequencing applications.

MagMAX™ Viral/Pathogen Binding Solution (Applied Biosystems™)

This binding buffer is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more binding buffer than is provided in the kit.

DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples (Invitrogen™)

DNAzol® Reagent is a complete and ready-to-use organic reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol® Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–100%. DNAzol® Reagent is:

Versatile—can use with a broad spectrum of sample materials
Efficient—rapid isolation and high recovery of genomic DNA

Efficient isolation of genomic DNA from a variety of sample types
The DNAzol® Reagent procedure uses a novel guanidine-detergent lysing solution that permits selective precipitation of DNA from cell lysate. 1 mL of DNAzol® Reagent can be used to isolate genomic DNA from 1–3 x 107 cells, from 0.1 mL of whole blood, or from 25–50 mg tissue.

Rapid isolation and high recovery of genomic DNA
The DNAzol® Reagent combines reliability, efficiency, and simplicity in its DNA isolation protocol. During isolation, a biological sample is lysed (or homogenized) in DNAzol® Reagent, and the genomic DNA is then precipitated from the lysate with ethanol. Following an ethanol wash, DNA may be solubilized in either water or 8 mM NaOH. The entire procedure can be completed in 10–30 min with DNA recovery of 70–100%.

Isolated DNA can be used for a number of downstream applications
Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.

TRIzol™ Reagent and Phasemaker™ Tubes Complete System (Invitrogen™)

This system includes both TRIzol™ Reagent and Invitrogen™ Phasemaker™ Tubes.

TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from a variety of biological samples. It offers superior lysis capability, even with difficult sample types.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA. This TRIzol Reagent and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.

MagMAX™ CORE Glass Microbeads (Applied Biosystems™)

MagMAX CORE Glass Microbeads are offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module. The beads aid in the homogenization of completely dessicated feces such as environmental samples or fecal pellets.

For a kit that contains these microbeads as well as the MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module, please see Cat. No. A37488.

Extra MagMAX FFPE DNA/RNA Ultra Reagents (Applied Biosystems™)

This package includes supplemental reagents for use with the MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881). These additional reagents consist of protease digestion buffer, binding solution, and DNA wash buffer and are specifically for use when processing >40 um sections. See the User Guide for details.

Plant DNAzol™ Reagent (Invitrogen™)

Plant DNAzol® Reagent is a ready-to-use organic reagent formulated for the isolation of high quality genomic DNA from a variety of plant samples.

• Permits use on broad spectrum plant material
• Rapid isolation of genomic DNA

RNA Hydrolysis for Efficient Isolation of Genomic DNA from a Variety of Sample Sources
Plant DNAzol® Reagent can be used for the efficient isolation of genomic DNA from a variety of plant tissues such as leaf, seed stem, root, and callus. The Plant DNAzol® procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate.

Isolate Genomic Plant DNA in About an Hour
In the Plant DNAzol® procedure, plant samples are pulverized in liquid nitrogen or homogenized, and genomic DNA is extracted from the homogenate with the Plant DNAzol® Reagent. Following extraction, plant debris is removed by centrifugation and DNA is precipitated from the supernatant with ethanol, and solubilized with TE buffer (pH 8.0). The entire procedure can be completed in approximately 60-70 minutes.

Comparable Yield in Less Time and with Fewer Reagents
Unlike traditional methods for plant DNA extraction (e.g., CTAB), Plant DNAzol® is quicker, does not require RNase A to remove RNA from genomic DNA preparations, and in the majority of tissues, does not require phenol. Furthermore, use of Plant DNAzol® generates yields from plant tissue that are comparable with the traditional CTAB method.

Isolated DNA can be used for a Number of Downstream Applications:
Amplified Fragment Length Polymorphism (AFLP)
Restriction Fragment Length Polymorphism (RFLP)
Southern Blotting
Sequencing
PCR
Cloning

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagMAX™ Cell-Free DNA Wash Solution (Applied Biosystems™)

This solution is included in the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). It is made available separately for applications that require more Wash Solution than is provided in the kit.

MagMAX™ Microbiome Bead Plate (Applied Biosystems™)

This bead plate is included in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. No. A42357). It is made available separately for applications that require additional plates.

DNAzol™ BD Reagent, for isolation of genomic DNA from whole blood (Invitrogen™)

DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes.

• Specifically formulated for extraction of genomic DNA from whole blood
• Optimized for the rapid Isolation of genomic DNA

Efficient Isolation of Genomic DNA from Whole Blood
DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. 1 ml of DNAzol® Reagent can be used to isolate genomic DNA from 0.5 ml of whole blood to yield 10-20 µg DNA. In addition to the isolation of genomic DNA, DNAzol® BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum.

Rapid Isolation of Genomic DNA
The DNAzol® Reagent combines reliability, efficiency and simplicity in its isolation protocol. In the procedure, blood samples are mixed with DNAzol® Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is then washed successively with DNAzol® Reagent followed by ethanol, and then solubilized. The entire procedure can be completed in about 30 min.

Isolated DNA can be Used for a Number of Downstream Applications:
Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagMAX™ Viral/Pathogen Wash Solution (Applied Biosystems™)

This wash solution is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more wash solution than is provided in the kit.

MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead plate (Applied Biosystems™)

Stool and soil are often the sample types of choice for scientists in the microbiome field. However, these samples also happen to be some of the most challenging to extract RNA and DNA from, until now. The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil. The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing.

Features of the MagMAX Microbiome Nucleic Acid Isolation Kit include:
• Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems
• Fast procedure allows for 96 samples to be processed in ~60 minutes
• Compatible with stool, soil, and swabs contained in transport medium
• Mechanical disruption via bead beating in 96-well plate or individual tubes
• Elution volumes ranging from 50 to 200 µL

Maximize sample input and nucleic acid recovery in minimal time
Up to 100 mg of stool can be processed per well in a KingFisher 96-deep well plate. When the extraction process is performed using a KingFisher Flex system, 96 samples can processed in ~60 minutes; when using a KingFisher Duo Prime system 12 samples can be processed in ~50 minutes. When the protocol is completed, pure RNA and DNA is concentrated in 100 µL of elution buffer.

Two bead beating formats available
High-throughput processing of difficult samples such as stool is possible with 96-deep well plates containing zirconia beads combined with optimized MagMAX Microbiome reagents (this page). If processing 96 samples at a time is not needed then the same beads are available in tube format for lower-throughput processing (Cat. No. A42358). Once the bead beating step is complete, the lysate can then be transferred into a KingFisher 96-deep well plate and processed on a KingFisher Flex or KingFisher Duo Prime system.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with stool samples that are commonly used for microbiome research.

The Dynabeads MyOne Silane contained in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery RNA and DNA. Once captured, these nucleic acids can then be eluted in of 50-100 µL of elution buffer for use in downstream applications.

PrepMan™ Ultra Sample Preparation Reagent (Applied Biosystems™)

The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step.

PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous fungi, both from a plate or from liquid culture, mammalian tissue smears, human cells (buccal swab), and mammalian whole blood. The resulting template has been used amplified in allelic discrimination assays, genetically modified organism (GMO) detection and quantification, food pathogen detection, microbial identification, and bacterial strain typing.

• Simple, rapid protocol reduces time to results.
• Unique formulation inactivates PCR inhibitors, yielding consistent results.
• PCR-ready template can be prepared from a wide range of sample types.
• Affordable method with a low price per preparation.
• Homogeneous solution with minimal sample-handling requirements.
• Microcentrifuge tube-based format means no additional equipment is needed for template preparation.

PrepMan® Ultra reagent is a novel formulation and is a homogeneous solution that does not contain Chelex® or any other type of resin or matrix.

This product is a replacement⁄improvement of PrepMan® Sample Preparation Reagent, and should be recommended to current and potential PrepMan® Reagent users.

Used With
3500/3500xL Genetic Analyzer Capillary Array (50 cm), 3130xL/3100 Genetic Analyzer Capillary Array (36 cm), 96-Well Plate Septa, AmpliTaq Gold® PCR Master Mix, BigDye® Terminator v1.1 Cycle Sequencing Kit, POP-6™ Polymer for 3100⁄3100-Avant™ Genetic Analyzers, PrepMan® Ultra Sample Preparation Reagent, TaqMan® GMO Maize 35S Detection Kit, TaqMan® GMO Soy 35S Detection Kit.

TRIzol™ Plus RNA Purification Kit and Phasemaker™ Tubes Complete System (Invitrogen™)

This system includes both the TRIzol™ Plus RNA Purification Kit and Invitrogen™ Phasemaker™ Tubes.

The TRIzol Plus RNA Purification Kit combines the strong lysis capability of TRIzol Reagent with the convenient and time-saving silica-cartridge purification protocol of the PureLink™ RNA Mini Kit to provide ultrapure total RNA typically within an hour from a wide variety of sample types.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying the removal of the upper phase containing the RNA. This TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.

MagMAX CORE Nucleic Acid Purification Kit + Mechanical Lysis Module (Applied Biosystems™)

This MagMAX CORE Mechanical Lysis Module Bundle includes a MagMAX CORE Mechanical Lysis Module and MagMAX CORE Nucleic Acid Purification Kit. The Mechanical Lysis Module is also available separately.

The Applied Biosystems MagMAX CORE sample preparation solution is a universal sample preparation system designed to meet current and future veterinary testing needs. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing. The MagMAX CORE Mechanical Lysis Module is offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of hard-to-lyse organisms like Mycobacterium avium paratuberculosis (MAP) and bovine tuberculosis in feces, milk, and environmental samples.

Features include:
Convenience—a complete solution using the same CORE chemistry
Confidence—higher-quality nucleic acids from difficult-to-lyse samples, including both small (0.3 g) and large (2–5 g) volume feces samples
Flexible—purchase only what you need to lyse MAP and other hard-to-lyse organisms

The MagMAX CORE Nucleic Acid Purification Kit and the MagMAX CORE Mechanical Lysis Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.

MagMAX™ Cell-Free DNA Lysis/Binding Buffer (Applied Biosystems™)

This buffer is included in the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). It is made available separately for applications that require more Lysis/Binding Buffer than is provided in the kit.