Shop All DNA⁄RNA Purification Reagents

MagMAX™ Microbiome Lysis Solution (Applied Biosystems™)

This lysis buffer is included in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42357, and A42358). It is made available separately for applications that require more lysis solution than is provided in the kit.

TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System (Invitrogen™)

This system includes both TRIzol™ LS Reagent and Invitrogen™ Phasemaker™ Tubes.

TRIzol LS Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from a variety of liquid biological samples such as serum and virus preparations.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA. This TRIzol LS Reagent and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate approximately 0.25 mL of sample.

MagMAX™ FFPE DNA/RNA Ultra Kit (Applied Biosystems™)

The MagMAX™ FFPE DNA/RNA Ultra Kit is designed for sequential isolation of DNA and RNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample. The DNA and RNA are recovered in separate eluates, and both are compatible with a broad range of applications, including real-time PCR and next-generation sequencing. The isolation of RNA and DNA from the same FFPE sample makes this kit an ideal method of sample preparation for Oncomine™ Comprehensive and Oncomine™ Focus assays.

Features of the MagMAX FFPE DNA/RNA Ultra Kit include:
• Flexible design that permits both manual and automated isolation of RNA and DNA
• Minimal requirement of 5 µm section or curl input
• Capable of processing 40 µm FFPE sections or curls; greater than 40 µm sections can be processed with an alternative protocol
• Compatible with targeted RNA and DNA sequencing panels
• Contains Dynabeads™ MyOne™ Silane for consistent isolation of RNA, microRNA, and DNA
• Alternative protocols for the isolation of RNA or DNA only

Unlocking the information contained in FFPE samples is essential to cancer research. Common nucleic acid isolation methods require you to choose which nucleic acid to recover from a given sample, with loss of the other nucleic acid (and any information that may have resulted from its analysis). Alternatively, the DNA and RNA are co-purified, or the sample is split in half so that each can be purified.

More information from less sample input
The MagMAX FFPE DNA/RNA Ultra Kit protocol addresses these issues by first isolating DNA from the FFPE sample and then recovering the RNA from the DNA-depleted supernatant. The nucleic acids are prepared as separate eluates and are then ready for downstream analysis. This allows for a fuller analysis of precious samples, including investigation of important biomarkers, such as hotspot mutations, CNVs, gene fusions, and indels.

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher™ Duo Prime or KingFisher™ Flex processor.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous FFPE tissue samples that that are not fully digested during the proteinase K treatment.

Optimized bead surface chemistry
The Dynabeads MyOne Silane utilized in the MagMAX FFPE DNA/RNA Ultra Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery of both RNA and DNA targets found in FFPE samples. Once captured, both of these nucleic acids are then eluted separately in volumes of 50 µL and are ready for downstream applications.

TRI Reagent™ Solution (Invitrogen™)

Tri Reagent® solution is a complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. Key features:

Simple—a single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein
Productive—obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods
Versatile—works with many sample sources, including human, plant, yeast, bacterial and viral samples
Flexible—easily scalable depending on starting material and desired yield

Using TRI Reagent® solution
TRI Reagent® starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability.

How it works
TRI Reagent® combines phenol and guanidine thiocyanate in a monophase solution to facilitate the immediate inhibition of RNase activity. Biological samples are homogenized or lysed in TRI Reagent®; the subsequent addition of bromochloropropane or chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The RNA can then be precipitated from the aqueous phase with the addition of isopropanol. The entire protocol can be completed in approximately 1 hour.

Applications
The isolated RNA is suitable for any downstream application, including RT-PCR, amplification for array analysis, hybridization assays, or in vitro translation. PCR, Southern blotting, and cloning can be performed using DNA isolated with TRI Reagent®. Isolated proteins can be used in SDS-PAGE and western blotting.

Tri Reagent® solution is incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA isolation kits.

MagMAX™ Cell-Free DNA Lysis/Binding Buffer (Applied Biosystems™)

This buffer is included in the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). It is made available separately for applications that require more Lysis/Binding Buffer than is provided in the kit.

PrepMan™ Ultra Sample Preparation Reagent (Applied Biosystems™)

The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step.

PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous fungi, both from a plate or from liquid culture, mammalian tissue smears, human cells (buccal swab), and mammalian whole blood. The resulting template has been used amplified in allelic discrimination assays, genetically modified organism (GMO) detection and quantification, food pathogen detection, microbial identification, and bacterial strain typing.

• Simple, rapid protocol reduces time to results.
• Unique formulation inactivates PCR inhibitors, yielding consistent results.
• PCR-ready template can be prepared from a wide range of sample types.
• Affordable method with a low price per preparation.
• Homogeneous solution with minimal sample-handling requirements.
• Microcentrifuge tube-based format means no additional equipment is needed for template preparation.

PrepMan® Ultra reagent is a novel formulation and is a homogeneous solution that does not contain Chelex® or any other type of resin or matrix.

This product is a replacement⁄improvement of PrepMan® Sample Preparation Reagent, and should be recommended to current and potential PrepMan® Reagent users.

Used With
3500/3500xL Genetic Analyzer Capillary Array (50 cm), 3130xL/3100 Genetic Analyzer Capillary Array (36 cm), 96-Well Plate Septa, AmpliTaq Gold® PCR Master Mix, BigDye® Terminator v1.1 Cycle Sequencing Kit, POP-6™ Polymer for 3100⁄3100-Avant™ Genetic Analyzers, PrepMan® Ultra Sample Preparation Reagent, TaqMan® GMO Maize 35S Detection Kit, TaqMan® GMO Soy 35S Detection Kit.

MagMAX™ Viral/Pathogen Elution Buffer (Applied Biosystems™)

This elution buffer is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more elution buffer than is provided in the kit.

MagMAX™ Viral/Pathogen Wash Solution (Applied Biosystems™)

This wash solution is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more wash solution than is provided in the kit.

MagMAX™ CORE Lysis Solution (Applied Biosystems™)

This MagMAX CORE Lysis Solution is made available as a supplement to that included in the MagMAX CORE Nucleic Acid Purification Kit. This proprietary buffer solution is used to break open cells in preparation for downstream molecular detection applications such as real time PCR, RT-PCR, and sequencing applications.

PureLink™ HiPure BAC Buffer Kit (Invitrogen™)

The PureLink® HiPure BAC Buffer Kit provides additional buffers and reagents for performing BAC (Bacterial Artificial Chromosome) DNA isolation using the PureLink® HiPure Plasmid Kits. The PureLink® HiPure Plasmid Kits utilize a proprietary anion-exchange resin which allows you to purify high-quality BAC DNA from E. coli cultures. BAC DNA requires the use of large bacterial culture volumes to obtain a reasonable DNA yield. The additional buffers and reagents needed for working with larger bacterial cultures are provided in this Buffer Kit. From a 100 ml culture, you can expect to purify ~40 µg of BAC DNA. The PureLink® HiPure BAC Buffer Kit provides reagents sufficient for five BAC Maxipreps, or 25 BAC Midipreps, or 100 BAC Minipreps.

TRIzol™ Reagent and Phasemaker™ Tubes Complete System (Invitrogen™)

This system includes both TRIzol™ Reagent and Invitrogen™ Phasemaker™ Tubes.

TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from a variety of biological samples. It offers superior lysis capability, even with difficult sample types.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA. This TRIzol Reagent and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.

MagMAX™ Microbiome Bead Tubes (Applied Biosystems™)

These bead tubes are included in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. No. A42358). They are made available separately for applications that require more tubes than are provided in the kit.

TRIzol™ Max™ Bacterial RNA Isolation Kit (Invitrogen™)

The TRIzol® Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol® Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.

Key Features of the TRIzol® Max™ Bacterial RNA Isolation Kit:

• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps

Two Products in One Kit
The TRIzol® Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol® Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol® Reagent generates a high-quality, minimally degraded RNA product.

Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol® Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

DNAzol™ BD Reagent, for isolation of genomic DNA from whole blood (Invitrogen™)

DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes.

• Specifically formulated for extraction of genomic DNA from whole blood
• Optimized for the rapid Isolation of genomic DNA

Efficient Isolation of Genomic DNA from Whole Blood
DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. 1 ml of DNAzol® Reagent can be used to isolate genomic DNA from 0.5 ml of whole blood to yield 10-20 µg DNA. In addition to the isolation of genomic DNA, DNAzol® BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum.

Rapid Isolation of Genomic DNA
The DNAzol® Reagent combines reliability, efficiency and simplicity in its isolation protocol. In the procedure, blood samples are mixed with DNAzol® Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is then washed successively with DNAzol® Reagent followed by ethanol, and then solubilized. The entire procedure can be completed in about 30 min.

Isolated DNA can be Used for a Number of Downstream Applications:
Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagMAX™ Cell-Free DNA Elution Solution (Applied Biosystems™)

This solution is included in the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). It is made available separately for applications that require more Elution Solution than is provided in the kit.