Shop All DNA⁄RNA Purification Reagents

MagMAX™ Viral/Pathogen Proteinase K (Applied Biosystems™)

This Proteinase K is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more Proteinase K than is provided in the kit.

MagMAX™ Viral/Pathogen Binding Solution (Applied Biosystems™)

This binding buffer is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more binding buffer than is provided in the kit.

PureLink™ Plant RNA Reagent (Invitrogen™)

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds). Features of the Plant RNA Reagent:

• Isolate high-quality RNA from difficult plant materials
• Use for small-scale and large-scale RNA isolations

Improved quality and purity of plant RNA
Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol® Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A260/A280 and gel analysis) from a variety of plant material sources.

Works for milligram to gram quantities of starting material
Plant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5 g plant tissue) total RNA isolation. The amount supplied (100 mL) is sufficient for 200 reactions using 100 mg of tissue, or 4 reactions using 5 g of tissue.

Isolated RNA is ideal for a variety of applications and products:

cDNA library construction kits
Real-Time PCR (qPCR)
Northern blotting kits & reagents
Poly(A) selection kit
RNase control reagents & assays

TRIzol™ Plus RNA Purification Kit and Phasemaker™ Tubes Complete System (Invitrogen™)

This system includes both the TRIzol™ Plus RNA Purification Kit and Invitrogen™ Phasemaker™ Tubes.

The TRIzol Plus RNA Purification Kit combines the strong lysis capability of TRIzol Reagent with the convenient and time-saving silica-cartridge purification protocol of the PureLink™ RNA Mini Kit to provide ultrapure total RNA typically within an hour from a wide variety of sample types.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying the removal of the upper phase containing the RNA. This TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.

MagMAX™ Viral/Pathogen Binding Beads (Applied Biosystems™)

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). They are made available separately for applications that require more binding beads than are provided in the kit.

MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes (Applied Biosystems™)

Stool and soil are often the sample types of choice for scientists in the microbiome field. However, these samples also happen to be some of the most challenging to extract RNA and DNA from, until now. The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil. The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing.

Features of the MagMAX Microbiome Nucleic Acid Isolation Kit include:
• Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems
• Fast procedure allows for 96 samples to be processed in ~60 minutes
• Compatible with stool, soil, and swabs contained in transport medium
• Mechanical disruption via bead beating in 96-well plate or individual tubes
• Elution volumes ranging from 50 to 200 µL

Maximize sample input and nucleic acid recovery in minimal time
Up to 100 mg of stool can be processed per well in a KingFisher 96-deep well plate. When the extraction process is performed using a KingFisher Flex system, 96 samples can processed in ~60 minutes; when using a KingFisher Duo Prime system 12 samples can be processed in ~50 minutes. When the protocol is completed, pure RNA and DNA is concentrated in 100 µL of elution buffer.

Two bead beating formats available
High-throughput processing of difficult samples such as stool is possible with 96-deep well plates containing zirconia beads combined with optimized MagMAX Microbiome reagents (Cat. No. A42357). If processing 96 samples at a time is not needed then the same beads are available in tube format for lower-throughput processing (this page). Once the bead beating step is complete, the lysate can then be transferred into a KingFisher 96-deep well plate and processed on a KingFisher Flex or KingFisher Duo Prime system.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with stool samples that are commonly used for microbiome research.

The Dynabeads MyOne Silane contained in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery RNA and DNA. Once captured, these nucleic acids can then be eluted in of 50-100 µL of elution buffer for use in downstream applications.

Agarase (0.5 U/µL) (Thermo Scientific™)

Thermo Scientific Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides. Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amplification, cloning, sequencing, etc.

MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead plate (Applied Biosystems™)

Stool and soil are often the sample types of choice for scientists in the microbiome field. However, these samples also happen to be some of the most challenging to extract RNA and DNA from, until now. The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil. The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing.

Features of the MagMAX Microbiome Nucleic Acid Isolation Kit include:
• Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems
• Fast procedure allows for 96 samples to be processed in ~60 minutes
• Compatible with stool, soil, and swabs contained in transport medium
• Mechanical disruption via bead beating in 96-well plate or individual tubes
• Elution volumes ranging from 50 to 200 µL

Maximize sample input and nucleic acid recovery in minimal time
Up to 100 mg of stool can be processed per well in a KingFisher 96-deep well plate. When the extraction process is performed using a KingFisher Flex system, 96 samples can processed in ~60 minutes; when using a KingFisher Duo Prime system 12 samples can be processed in ~50 minutes. When the protocol is completed, pure RNA and DNA is concentrated in 100 µL of elution buffer.

Two bead beating formats available
High-throughput processing of difficult samples such as stool is possible with 96-deep well plates containing zirconia beads combined with optimized MagMAX Microbiome reagents (this page). If processing 96 samples at a time is not needed then the same beads are available in tube format for lower-throughput processing (Cat. No. A42358). Once the bead beating step is complete, the lysate can then be transferred into a KingFisher 96-deep well plate and processed on a KingFisher Flex or KingFisher Duo Prime system.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with stool samples that are commonly used for microbiome research.

The Dynabeads MyOne Silane contained in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads MyOne Silane ideal for the recovery RNA and DNA. Once captured, these nucleic acids can then be eluted in of 50-100 µL of elution buffer for use in downstream applications.

MagMAX™ Cell-Free Total Nucleic Acid Isolation Kit (Applied Biosystems™)

The MagMAX Cell-Free Total Nucleic Acid Isolation Kit is designed for enrichment of all nucleic acid species present in cell-free biological samples such as plasma. The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.

The magnetic bead–based purification format allows you to easily process a wide range of sample input volumes (1 mL to 6 mL). When used with the KingFisher Duo Prime or KingFisher Flex Magnetic Particle Processor, 6 to 24 plasma samples of 2-mL input volume can be isolated at once. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX Cell-Free Total Nucleic Acid Isolation Kit include:
• Automation-ready, phenol-free extraction
• Fast procedure allows 6 to 24 samples to be processed in 90 minutes or less when used with the KingFisher Duo Prime or KingFisher Flex Magnetic Particle Processor with a 24 Deep Well Head
• Flexible protocol accommodates sample volume inputs ranging from 1 mL to 6 mL of plasma
• No need for carrier RNA
• Elution volumes ranging from 15 µL to 60 µL
• Compatible with the Oncomine Lung cfTNA Research Assay

Go beyond cell-free DNA and get the total picture
Isolating and analyzing circulating cell-free total nucleic acid (cfTNA) from plasma and other cell-free samples serves as a non-invasive method to detect and monitor targets associated with certain disease states such as cancer.

The first step in attaining this valuable information is efficient nucleic acid isolation that specifically recovers the circulating nucleic acid species that contains information related to the disease state. The importance of cell-free DNA (cfDNA) is becoming established, but it may not tell the whole story. Cell-free RNA (cfRNA) sequences serve as critical biomarkers as well in the form of microRNAs and gene fusions. This inclusion of the cfRNA with the cfDNA provides an opportunity for researchers to expand their studies and advance human health.

The MagMAX Cell-Free Total Nucleic Acid kit is developed by the same Thermo Fisher Scientific scientists that brought you the MagMAX Cell-Free DNA Isolation Kit. The difference is the inclusion of all cfRNA species in the eluate, allowing you to increase the amount of information available from a single cell-free sample. Just like the cfDNA Isolation Kit, the cfTNA kit is designed for both manual and automated isolation of nucleic acid from a wide sample volume input range of 1 to 6 mL. The phenol-free, magnetic-bead format makes the kit fully amenable to automated isolation.

Thanks to the scalable format that allows for various sample inputs, it is possible to vary the number of samples processed with a single kit. For example, enough reagents are provided to process 50 samples of 2 mL plasma each. Alternatively, with the same kit it is possible to process 25 samples of 4 mL plasma each. Higher and lower input volumes are possible and the number of samples that can be processed will vary accordingly. This unique product attribute allows you to tailor the product to your specific needs.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with protein-rich, large-volume samples such as plasma that are commonly used in circulating cell-free nucleic acid studies.

Max Bacterial Enhancement Reagent (Invitrogen™)

The Max Bacterial Enhancement Reagent is designed for use with TRIzol® Reagent to improve the isolation of intact total RNA up to 3-fold from Gram-positive and Gram-negative bacteria. The Max Bacterial Enhancement Reagent is a ready-to-use solution composed of chelating agents, detergent, and a buffer, and is used as an efficient pre-treatment buffer for bacterial cells prior to RNA isolation with TRIzol® Reagent. The use of Max Bacterial Enhancement Reagent with TRIzol® inactivates endogenous RNases and promotes protein denaturation, improving the RNA quality and integrity. The Max Bacterial Enhancement Reagent is available either separately or packaged with TRIzol as part of the TRIzol® Max™ Bacterial RNA Isolation Kit (Cat. No. 16096020 or 16096040).