Shop All β-Gal (lacZ) Assays

Yeast beta-Galactosidase Assay Kit (Thermo Scientific™)

Thermo Scientific Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in-vivo using two-hybrid systems.

Features of Yeast beta-Galactosidase Assay Kit:

• Efficient lysis of yeast cells and a colorimetric detection system
• Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
• Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
• Adaptable to 96-well microplates or tube assay formats
• Allows cells obtained from solid media or liquid culture to be assayed directly with no harvesting or washing steps.• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatible with prepared lysates from other species such as bacteria

Yeast β-Galactosidase Assay Kit allows qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER Yeast Protein Extraction Reagent and β-Galactosidase Assay Buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm.

The gene encoding β-galactosidase (lacZ) of E. coli has been widely used as a reporter gene in many different prokaryotic and eukaryotic organisms. β-Galactosidase activity is also commonly used as an indicator of protein-protein interactions in-vivo using two-hybrid systems. The interaction strength is verified or quantitated using a β-galactosidase activity assay.

beta-Galactosidase Assay Reagent (Thermo Scientific™)

The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

Related Products
Mammalian beta-Galactosidase Assay Kit
beta-Galactosidase Assay Stop Solution

Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells (Invitrogen™)

The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

• Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range.
• Assay sensitivity is 100 to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Ideal for Screening
This homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen® system uses Galacton-Star® chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

For Research Use Only. Not for use in diagnostics procedures.

beta-Galactosidase Assay Stop Solution (Thermo Scientific™)

The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

Related Products
Mammalian beta-Galactosidase Assay Kit
beta-Galactosidase Assay Reagent

Mammalian beta-Galactosidase Assay Kit (Thermo Scientific™)

The Thermo Scientific Mammalian β-Galactosidase Assay Kit provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

Features of the Mammalian β-Galactosidase Assay Kit:

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Related Products
beta-Galactosidase Assay Reagent
beta-Galactosidase Assay Stop Solution

ImaGene Green™ C12FDG lacZ Gene Expression Kit (Invitrogen™)

The ImaGene Green™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12FDG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12FDG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.

FluoReporter™ lacZ Flow Cytometry Kit (Invitrogen™)

The FluoReporter® lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in single cells using flow cytometry. This kit is accompanied by a license to practice patented techniques for loading FDG by hypotonic shock and improving retention of fluorescein in lacZ-positive cells.

ImaGene Red™ C12RG lacZ Gene Expression Kit (Invitrogen™)

The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.

β-Gal Staining Kit (Invitrogen™)

The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of X-gal, producing a blue color that can be easily visualized under a microscope.

Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System (Invitrogen™)

The Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of β-galactosidase in reporter gene assays.

• The Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Ready-to-Use
The Galacto-Light™ Plus™ system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.

Products described herein are guaranteed for 12 months from the date of purchase.

For Research Use Only. Not for use in diagnostics procedures.

FluoReporter™ lacZ/Galactosidase Quantitation Kit (Invitrogen™)

The FluoReporter® lacZ/Galactosidase Quantitation Kit includes the β-galactosidase substrate, 3-carboxyumbelliferyl β-D-galactopyranoside that produces the blue-fluorescent hydrolysis product, 7-hydroxycoumarin-3-carboxlic acid.

Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells (Invitrogen™)

The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

• Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range.
• Assay sensitivity is 100 to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Ideal for Screening
This homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen® system uses Galacton-Star® chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

For Research Use Only. Not for use in diagnostics procedures.

Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells (Invitrogen™)

The Galacto-Star™ One-Step β-galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.

• Glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Versatile
The Galacto-Star™ system includes Galacton-Star® chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.

Long-Lasting Signal
Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star® substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.

For Research Use Only. Not for use in diagnostics procedures.

DetectaGene™ Green CMFDG lacZ Gene Expression Kit (Invitrogen™)

The DetectaGene™ Green lacZ Gene Expression Kit includes a fluorescein-based β-galactose derivative that has been chemically modified to include a mildly thiol-reactive chloromethyl group. Once inside a cell, the DetectaGene™ substrate undergoes two reactions: 1) its two galactose moieties are cleaved by intracellular β-galactosidase and 2) either simultaneously or sequentially, its chloromethyl moiety reacts with glutathione and possibly other intracellular thiols to form a membrane-impermeant, peptide-fluorescent dye adduct.

β-Gal Assay Kit (Invitrogen™)

The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG). Hydrolysis of ONPG to the ONP anion produces a bright yellow color with a peak absorbance at 420 nm that can be quantified using a spectrophotometer.
10.7 gene expression