Shop All Kinase Assay Kits

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.

Z'-LYTE™ Kinase Assay Kit - Tyrosine 7 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon LAT, containing Tyr-200.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 14 Peptide

A coumarin and fluorescein-labeled serine⁄threonine peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is derived from a peptide substrate for PAK.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S⁄T)-P consensus sequence, where X is any residue.

Z'-LYTE™ Kinase Assay Kit - Tyrosine 6 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for EGF Receptor.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 15 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon EGFR, containing Thr-693.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 20 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a synthetic peptide substrate for NDR1.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S⁄T-X-X, where Hyd is bulky hydrophobic, F > L > V>>A, and X is any residue.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 24 Peptide

A coumarin and fluorescein-labeled serine⁄threonine peptide substrate which can be used for kinase screening in an antibody-free format.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 9 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase I, containing Ser-641.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 16 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDC25C, containing Ser-198.

Kinase Tracer 222, 50 µM

Kinase Tracer 222 is an Alexa Fluor® 647 dye-labeled, ATP-competitive kinase inhibitor scaffold for use in the LanthaScreen® Eu Kinase Binding Assay.

The LanthaScreen® Eu Kinase Binding Assay
Is easily optimized
Measures inhibitor binding
Performs simple off-rate measurements

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.

Kinase Tracer 314, 25 µM

In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or “tracer" such as Kinase Tracer 314 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Learn More and See Which Kinases Have Been Validated Using This Assay

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 22 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-161.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 23 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a PKC epsilon pseudosubstrate derived peptide.