Shop All Streptavidin-Coupled Magnetic Beads

MagnaBind™ Streptavidin Beads (Thermo Scientific™)

Thermo Scientific MagnaBind Streptavidin Beads are convenient for affinity purification or separation methods involving of biotin-labeled molecules. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

Features of MagnaBind Streptavidin Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: 5 mg/mL

Streptavidin is a 60kDa protein from Streptomycetes avidinii. The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine• HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

Dynabeads™ M-280 Streptavidin (Invitrogen™)

Dynabeads® M-280 Streptavidin are the gold standard for the isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Low-charged and neutral beads, optimal for binding of DNA fragments, proteins, peptides, and antibodies
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securing consistent results in your application

About Dynabeads® M-280 Streptavidin
These uniform and superparamagnetic beads are 2.8 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results.Applications
Over the past 15 years, Dynabeads® M-280 Streptavidin have been used and cited for a very wide variety of applications. Key applications include preparing 2–5 kb samples for mate pair library sequencing using Illumina platforms preparing single-stranded DNA templates, isolation of RNA and DNA binding proteins, immobilization of large DNA fragments, purifying sequencing products, and the specific capture of nucleic acids. Dynabeads® are used on more than 25,000 routine IVD instruments worldwide. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

Binding capacity
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-280 Streptavidin typically binds:

• 650–900 pmoles free biotin
• ~200 pmol biotinylated peptides
• ~10 µg biotinylated IgG
• ~10 µg ds-DNA
• ~200 pmol ss-oligonucleotides

Dynabeads™ M-280 Streptavidin, for OEM and industrial use only

Uniform and superparamagnetic Dynabeads®, 2.8 µm in diameter, with a monolayer of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The beads allow for efficient capture and downstream handling of your biotinylated ligand or target molecule. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results.

Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated antibodies, nucleic acids or other biotinylated ligands and targets. The liquid-phase behavior in combination with the superparamagnetic properties of the beads provide rapid reaction kinetics both in the coating process, separation and during washing of the analyte.

The product holds reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.

Benefits and Features:

• Direct and fast capture of any biotinylated ligand or target
• Flexible solid-phase protocols with superior liquid-phase reaction kinetics
• Easy and reproducible handling in manufacturing
• Low charged and neutral beads, optimal for binding of proteins, peptides and antibodies
• Fast and efficient washing procedures
• High batch-to-batch reproducibility, securing consistent results in your application
• Well suited for automated protocols

Applications:
Efficient capture of biotinylated molecules. For direct⁄indirect isolation and downstream handling of nucleic acids, proteins⁄peptides and other target molecules. Due to their uniformity, excellent batch reproducibility and ease of adaptation to automated processes, Dynabeads® have become the solid phase of choice for immunoassay development.

Binding capacity:
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-280 Streptavidin typically binds 650-900 pmoles free biotin, 200 pmol biotinylated peptides, up to10 µg biotinylated antibody, approx. 10 µg ds-DNA or 200 pmol ss-Oligonucleotides.

Additional Info:
This specific product format is for large volume customers, available on an OEM basis. The product is also available in smaller volumes for end-users (Cat. no 112-05D, 112-06D and 602-10).

Pierce™ Streptavidin Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Magnetic Beads accelerate throughput for automated magnetic purification of biotinylated molecules.

Features of Streptavidin Magnetic Beads:

High-performance beads—non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles provide exceptional uniformity for automated HTS and manual applications alike
Stable immobilization chemistry—streptavidin is immobilized using leach-resistant chemistry
High capacity—superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples
Low non-specific binding—stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with biotinylated antibody) that are compatible with mass spectrometry analysis and other downstream analyses
Superior performance—nearly three times higher binding capacity than typical beads from other suppliers, allowing the use of smaller amounts per experiment

Applications:
• Immunoprecipitate antigens (using biotinylated antibodies) from a wide variety of sources
• Co-immunoprecipitate interaction complexes using biotinylated antibodies
• Capture protein-protein interactions in pull-down assays using biotinylated "bait" proteins
• Isolate biotin-labeled DNA-protein complexes from cell or tissue extracts
• Capture single-stranded biotinylated DNA oligos
• Isolate biotinylated PCR products

These streptavidin magnetic beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand. The iron oxide, super-paramagnetic particles offer superior performance (high capacity and low nonspecific binding) compared with other commercial magnetic beads.

Pierce Streptavidin Magnetic Beads use a recombinant form of streptavidin with a mass of 53kDa and a near-neutral isoelectric point (pI). The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptavidin has no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine·HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.

MagniSort™ Streptavidin Positive Selection Beads (Invitrogen™)

MagniSort™ Streptavidin Positive Selection Beads are designed for the magnetic separation of cells by positive selection. Desired cells are bound by biotinylated antibody and then streptavidin-coated magnetic beads. When placed in a magnetic field, the desired cells are held in place and the undesired cells can be separated by decanting. The clone and concentration of biotinylated antibody to be used to label the desired cells, as well as the concentration of MagniSort™ Streptavidin Positive Selection Beads must be determined empirically. Please refer to the protocol for details on how to optimize both the antibody and MagniSort™ Streptavidin Positive Selection Beads.

The MagniSort™ Streptavidin Positive Selection Beads may also be used to deplete undesired cells using a single antibody. For depletion of cells using multiple antibodies, we recommend the MagniSort™ Streptavidin Negative Selection Beads (cat. MSNB-6002) and protocol.

Reported Application
Magnetic Cell Separation

Dynabeads™ MyOne™ Streptavidin T1 (Invitrogen™)

Dynabeads MyOne Streptavidin T1 are the gold standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Low-charged and neutral beads, optimal for binding of proteins, peptides, and antibodies
• Their small but uniform size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule
• Low sedimention rate, yet a high iron content, ensuring rapid magnetic separation
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securing consistent results in your application

About Dynabeads MyOne Streptavidin T1
These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. The beads show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. The 1 µm Dynabeads MyOne have a large surface area, high capacity, efficient magnetic pull, and a slow sedimentation rate during incubation. Tailor-made for use in automated protocols where high throughput is crucial.

Applications
Over the past 15 years, streptavidin-coupled Dynabeads have been used and cited for a very wide variety of applications. Key applications include preparing single-stranded DNA templates, isolation of RNA and DNA binding proteins, immobilization of large DNA fragments, purifying sequencing products, and the specific capture of nucleic acids. Easily adapted to automated processes. Dynabeads are used on more than 25,000 routine IVD instruments worldwide.

Binding capacity
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads MyOne Streptavidin T1 typically binds:

• 950–1500 pmoles free biotin
• ~20 µg biotinylated IgG
• ~400 pmol biotinylated peptides
• ~20 µg ds-DNA
• ~400 pmol ss-oligonucleotides

Dynabeads™ Streptavidin Trial Kit (Invitrogen™)

Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated molecules and targets (e.g. antibodies, proteins, peptides, small molecules, sugars, lectins, oligonucleotides and DNA/RNA).

The beads are available with different sizes and surface properties, This low-cost Trial Kit allows you to test the flexible Dynabeads® in your application

Applications:
The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is utilized in a vast number of applications. Over the past 15 years, Streptavidin-coupled Dynabeads® have been widely used and cited. Examples include direct/indirect capture, separation and downstream handling of nucleic acids, purification of DNA/RNA binding proteins, proteins/peptides, as well as protein interaction studies, immunoprecipitation, immunoassays, phage display, biopanning, drug screening and cell isolation. Did you know that Dynabeads® are employed on more than 25,000 routine IVD instruments worldwide?

Benefits and Features:

Direct and fast isolation of any biotinylated molecule

• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• No need for filters, columns or centrifugations
• Manual or automated - protocols can easily be adjusted from manual to automated use on any laboratory robot that can be equipped with a magnet
• Rapid liquid-phase kinetics give efficient capture with a high signal-to-noise ratio and increased sensitivity
• High batch-to-batch reproducibility, securing consistent results in your application

The uniform and superparamagnetic Dynabeads® Steptavidin have a monolayer - not a multilayer - of recombinant streptavidin covalently coupled to their surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. Their specific and defined surface allow for efficient capture, separation and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency.

The product holds reputable Dynal high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

More Product Information:
Please refer to the product descriptions for the 4 different bead-types in this trial kit: Dynabeads® M-280 Streptavidin, Dynabeads® M-270 Streptavidin, Dynabeads® MyOne™ Streptavidin C1 and Dynabeads® MyOne™ Streptavidin T1. See Product Systems at right of this page for other Dynabeads® Streptavidin products

Dynabeads™ kilobaseBINDER™ Kit (Invitrogen™)

Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated nucleic acids, antibodies or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is utilized in a vast number of applications. The amount of biotinylated DNA immobilized will depend on fragment size. Due to steric hindrance, the binding efficiency is significantly reduced when the fragment size exceeds 2 kb. The specially designed Binding Solution supplied in the Dynabeads® kilobaseBINDER™ Kit contains a patented immobilization activator that significantly increases the binding capacity for large double stranded DNA fragments.

Applications:
The Dynabeads® kilobaseBINDERTM Kit is designed for immobilizing long double stranded DNA molecules (>2 kb). This product has been useful for innovative applications in cell biology (e.g. building chromatin beads that mimic chromosomes for the study of self-organization of microtubules into bipolar spindles, building synthetic nuclei for studying RNA export from the nucleus in vitro, reconstituting nuclear movement along microtubules and examination of the molecular basis of this movement in vitro. A reference list is available upon request.

Benefits and Features:

• Increased binding capacity for >2 kb dsDNA molecules
• Chromatin isolation within minutes
• High batch-to-batch reproducibility, securing consistent results in your application

Binding capacity:
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. The Dynabeads® are coated with a monolayer of recombinant streptavidin. This leaves the majority of the biotin binding sites sterically available for binding not only of free biotin, but also biotinylated ligands/targets.

The streptavidin monolayer is covalently coupled to the beads, ensuring negligible leakage and superior reproducibility. With the unique Binding Solution supplied in this kit, it is possible to immobilize 70 pmoles of a 4 kb biotinylated DNA fragment and 80 pmoles of a 10 kb fragment per mg beads.The Invitrogen Dynal quality control tests ensure the following binding capacity criteria to be fulfilled:
• >10 times better capacity for binding of a biotinylated 4 kb DNA fragments per mg beads when using the Binding Solution compared to no use of Binding Solution.
• >195 μg (6 pmol) biotinylated 50 kb DNA fragment per mg beads.
In addition, the EMBL, Heidelberg group who developed the Binding Solution has reported the following results:
• Approximately 400 μg (100 pmole) biotinylated 6.2 kb DNA fragments per mg Dynabeads M-280 Streptavidin.

The product holds reputable Dynal high standards with respect to reproducibility (both within ad between batches), and drives reliability for your results.

MagniSort™ Streptavidin Negative Selection Beads (Invitrogen™)

MagniSort™ Streptavidin Negative Selection Beads are designed for the magnetic separation of cells by negative selection. Undesired cells are bound by a cocktail of biotinylated antibodies and then streptavidin coated magnetic beads. When placed in a magnetic field, the undesired cells are held in place and the desired cells can be separated by decanting. The clone and concentration of biotinylated antibody to be used to label the undesired cells, as well as the concentration of MagniSort™ Streptavidin Negative Selection Beads must be determined empirically. Please refer to the protocol for details on how to optimize both the antibody or antibody cocktail and MagniSort™ Streptavidin Negative Selection Beads.

For depletion of cells using a single antibody, we recoomend the MagniSort™ Streptavidin Positive Selection Beads (cat. MSPB-6003) and protocol.

Reported Application
Magnetic Cell Separation

Dynabeads™ MyOne™ Streptavidin C1 (Invitrogen™)

Dynabeads® MyOne™ Streptavidin C1 are the gold standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Well suited for nucleic acid applications with extreme demands
• Very low nonspecific binding of nucleotides and nucleic acids
• Low nonspecific binding of small and negatively charged proteins
• Their small but uniform size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule
• Low sedimention rate, yet a high iron content, ensuring rapid magnetic separation
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securing consistent results in your application
• Production follows a validated process in compliance with cGMP for medical devices

About Dynabeads® MyOne™ Streptavidin C1
These uniform and superparamagnetic beads are 1 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They are hydrophilic, negatively charged, and show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. The 1 µm Dynabeads® MyOne™ have a large surface area, high capacity, efficient magnetic pull and a slow sedimentation rate during incubation. Tailor-made for use in automated protocols where high throughput is crucial. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

Applications
Over the past 15 years, Streptavidin-coupled Dynabeads® have been used and cited for a very wide variety of applications. Examples include direct/indirect isolation and downstream handling of nucleic acids, proteins/peptides and other target molecules. Ideal for sequence specific DNA/RNA capture in nucleic acid based diagnostics, specifically with samples with a high chaotropic salt concentration, immunoassays involving small biotinylated antigens and applications that are not compatible with BSA (these beads are not blocked with BSA). Easily adapted to automated processes. Did you know that Dynabeads® are employed on more than 25,000 routine IVD instruments worldwide?

Binding capacity
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® MyOne™ Streptavidin C1 typically binds:

• >2,500 pmoles free biotin
• ~20 µg biotinylated IgG
• ~400 pmol biotinylated peptides
• ~20 µg ds-DNA
• ~500 pmol ss-oligonucleotides

Dynabeads™ M-270 Streptavidin (Invitrogen™)

Dynabeads® M-270 Streptavidin the gold standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Very low nonspecific binding of nucleotides and nucleic acids
• Very low aggregation in high-salt hybridization buffers
• Well suited for nucleic acid applications with extreme demands
• Low nonspecific binding of small and negatively charged proteins
• Production follows a validated process in compliance with cGMP for medical devices
• Well suited for automated protocols

About Dynabeads® M-270 Streptavidin
These uniform and superparamagnetic beads are 2.8 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They are hydrophilic, negatively charged and show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. Applications
Over the past 15 years, streptavidin-coupled Dynabeads® have been used and cited for a very wide variety of applications. Examples include direct/indirect isolation and downstream handling of nucleic acids, proteins/peptides, and other target molecules. Ideal for sequence-specific DNA/RNA capture in nucleic acid–based diagnostics, specifically with samples with a high chaotropic salt concentration, immunoassays involving small biotinylated antigens, and applications that are not compatible with BSA (these beads are not blocked with BSA). Easily adaptated to automated processes. Dynabeads® are used on more than 25,000 routine IVD instruments worldwide. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

Binding capacity
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-270 Streptavidin typically binds:

• >950 pmoles free biotin
• ~200 pmol biotinylated peptides
• ~10 µg biotinylated IgG
• ~10 µg ds-DNA
• ~200 pmol ss-oligonucleotides