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LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15127:

The LanthaScreen® TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format.

The assay
To run the LanthaScreen® TR-FRET VDR Coactivator Assay, VDR-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based upon the biology of the VDRcoactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET VDR Coactivator Assay Kit contains the VDR-LBD (GST) protein, fluorescein-labeled TRAP220/DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

LanthaScreen™ TR-FRET Glucocorticoid Receptor Competitive Binding Kit

The LanthaScreen® TR-FRET Glucocorticoid Receptor Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for glucocorticoid receptor (GR). The kit uses a human GR ligand-binding domain (GR-LBD) tagged with glutathione S-transferase (GST) (also available separately), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule GR ligand (Fluormone™ GS1) in a homogeneous mix-and-read assay format.

The Lanthascreen Gluococorticoid Receptor Competitive Binding Assay is:

• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—TR-FRET assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs

EnzChek™ Phosphate Assay Kit (Invitrogen™)

The EnzChek® Phosphate Assay Kit provides a fast and sensitive spectrophotometric method for the quantification of inorganic phosphate in solution and for phosphate released during ATPase or GTPase enzymatic reactions.

See our complete line of Fluorescence Microplate assays.

• Sensitivity of 2 to 150 µM phosphate
• Spectrophotometric microplate-based assay detects a change in absorbance maximum from 330 nm to 360 nm
• Continuous monitoring of phosphate generated by ATPase or GTPase activity

In the presence of inorganic phosphate, the 2-amino-6-mercapto-7-methylpurine riboside (MESG) substrate is converted by the purine nucleoside phosphorylase (PNP) enzyme to the ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine product. This enzymatic conversion of MESG results in a spectrophotometric shift in maximum absorbance from 330 nm for the substrate to 360 nm for the product.

Sensitivity of the assay is in the range of 2 to 150 µM inorganic phosphate (2 to 150 nanomoles phosphate in a 1 mL volume), and the reaction can be performed over a pH range of 6.5 to 8.5. The EnzChek® phosphate reaction is sufficiently fast and quantitative for use in stopped-flow kinetic experiments.

Ferric Antioxidant Status Detection Kit (Invitrogen™)

The Ferric Antioxidant Status Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of Ferric Antioxidant Status (also referred as Ferric Reducing Antioxidant Power, FRAP) in serum, plasma, urine, teas, fruit juices, beer, cider, cell lysates, herbal and fruit extracts.

This complete, ready-to-use kit includes clear 96-well plate(s), ferrous chloride standard, ascorbic acid control, and other components to perform the assay. A 96-well microplate reader capable of reading optical density at 560 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, urine, teas, fruit juices, beer, cider, cell lysates, herbal and fruit extracts
• Sensitivity: 8.06 uM
• Standard curve range: 31.2 uM–1,000 uM
• Reactivity: species independent

Background
Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism. These free radicals (FR) are usually removed or converted into other products in vivo by an array of antioxidants. Organisms have developed complex antioxidant systems to protect themselves from oxidative stress, including the activity of specific enzymes (especially superoxide dismutase, glutathione peroxidase, catalase, glutathione reductase) as well as nonenzymatic compounds with antioxidant activity, such as beta-tocopherol, L-ascorbic acid, glutathione, coenzyme Q10, flavonoids, and albumin. The assay measures the antioxidant ability from all species.

Assay principle
The Ferric Antioxidant Status Detection kit is designed to quantitatively measure antioxidant status in a variety of samples. A ferrous chloride standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in assay buffer and added to the wells. The FRAP color solution is made by mixing reagent A and B with assay buffer. The FRAP color solution is added to all wells and the plate incubated at room temperature. Antioxidant power in the samples reacts with the FRAP color solution to generate a blue-colored product which is read at 560 nm.

Related links
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LanthaScreen™ TR-FRET Progesterone Receptor Coactivator Assay Kit

The LanthaScreen® TR-FRET Progesterone Receptor Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential progesterone receptor (PR) ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a PR ligand--binding domain (PR-LBD) tagged with glutathione-S-transferase (GST) (also available separately), a terbium (Tb)-labeled anti-GST antibody, and a fluorescein-labeled coactivator peptide in a homogenous mix-and-read assay format.

Agonist Mode
When using the LanthaScreen TR-FRET Progesterone Receptor Coactivator Assay in agonist mode (to identify agonist compounds), PR-LBD is added to ligand test compounds, followed by the addition of a mixture of fluorescein-labeled coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the TR-FRET ratio of 520/495 nm is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PR-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see figure).

Antagonist Mode
When using the LanthaScreen™ TR-FRET Progesterone Receptor Coactivator Assay in antagonist mode (to identify antagonist compounds), PR-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluorescein-labeled coactivator peptide, and terbium-labeled anti-GST antibody (see figure). The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode.

LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15140:

The LanthaScreen® TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Formaldehyde Fluorescent Detection Kit (Invitrogen™)

The Formaldehyde Detection research-use-only kit is a fluorescent assay designed for the quantification and detection of formaldehyde in human urine and tissue culture media samples.

This complete, ready-to-use kit includes clear 96-well plate(s), formaldehyde standard, formaldehyde detection reagent, and other components to perform the assay. A 96-well fluorescence plate reader capable of reading fluorescent emission at 510 nm, with excitation at 450 nm, is required for use of this kit.

Performance characteristics
• Assay type: fluorescent detection Kit
• Sample types: human urine and tissue culture media samples
• Sensitivity: 0.715 uM
• Standard curve range: 3.1 uM–200 uM
• Reactivity: species independent

Background
Formaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent. Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. This kit was shown to measure formaldehyde in human urine and tissue culture media. Formaldehyde is identical across all species and cell types.

Assay principle
The Formaldehyde Urinary Detection kit designed to quantitatively measure formaldehyde present in tissue culture media and urine samples. A formaldehyde standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a black microtiter plate. The fluorescent reaction is initiated with the formaldehyde detection reagent, which is pipetted into each well. After a short incubation the emission of the generated fluorescent signal is detected in a microtiter plate reader capable of measuring 510 nm fluorescence utilizing 450 nm excitation wavelength.

Related links
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Learn more about other immunoassays

Galactose Colorimetric Detection Kit (Invitrogen™)

The Galactose Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of galactose in serum, plasma, buffers and tissue culture media.

This complete, ready-to-use kit includes clear 96-well plate(s), galactose standard, assay buffer, and other components to perform the assay. A 96-well microplate reader capable of reading optical density at 560 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, buffers, and tissue culture media
• Sensitivity: 0.493 mg/dL
• Standard curve range: 0.78 mg/dL–25 mg/dL
• Reactivity: species independent

Background
Galactose is a hexose sugar that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Present as an anomeric mixture of alpha-D-galactose and beta-Dgalactose, this monosaccharide exists abundantly in milk, dairy products, and many other food types such as fruits and vegetables. Absorption of galactose in humans is mediated by the Na+/glucose co-transporters SGLT1 and SGLT2 from food across the brush border membrane of the proximal jejunum and renal epithelium. Other sources of galactose include endogenous production and natural turnover of glycolipids and glycoproteins.

Inside the cells, beta-D-galactose is epimerized to alpha-D-galactose through the action of a mutarotase. Alpha-D-galactose is subsequently converted to galactose-1-phosphate (Gal-1-P) by the enzyme galactokinase. In the presence of galactose-1-phosphate uridylyltransferase, Gal-1-P reacts with UDP-glucose to form UDP-galactose and glucose-1-phosphate. Glucose-1-phosphate produced can enter the glycolytic pathway or react with UTP in the presence of UDP-glucose pyrophosphorylase to form a new molecule of UDP-glucose. This enzyme pathway comprises the evolutionarily conserved Leloir pathway of galactose metabolism.

Assay principle
The Galactose Colorimetric Detection Kit is designed to quantitatively measure galactose in a variety of samples. A D-(+)-galactose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Substrate and horseradish peroxidase and the reaction initiated by addition of galactose oxidase. The plate is incubated at room temperature for 30 minutes. The galactose oxidase reacts with galactose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorless Substrate to produce a pink-colored product to be read at 560 nm. Increasing levels of galactose cause a linear increase in color.

Related links
Learn more about ELISA kits
Learn more about other immunoassays

Vivid™ CYP2C9 Green Screening Kit

Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid® CYP450 Screening Kits offer:
• Easy three-step procedure, "mix and read" format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well

Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP2C9 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® BOMF Substrate is the substrate included in this particular kit. This substrate yields a product that emits a green fluorescence.

Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green

The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands. The kit uses insect cell-expressed, human PR ligand binding domain tagged with glutathione-S-transferase (GST) (also available separately) and a novel, high-affinity, fluorescent progesterone receptor ligand (Fluormone™ PL Green) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a "red" kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact us at drugdiscoverytech@lifetech.com.

The PolarScreen Progesterone Receptor Competitor Assay is:

• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—fluorescence polarization assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs

How the Assay Works
An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently-tagged progesterone ligand (Fluormone PL Green) in the presence of competitor test compounds in microwell plates. The presence of effective competitors prevents the formation of a PL Green/PR-LBD(GST) complex, resulting in a decrease of the polarization value. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for PR-LBD(GST).

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Predictor™ hERG Fluorescence Polarization Assay Kit

The Predictor™ hERG Fluorescence Polarization Assay Kit provides a set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand. The assay was designed to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies. The assay is based on the principle of fluorescence polarization where a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. Assay performance was validated with a panel of established hERG channel blockers. Results from the Predictor™ assay demonstrate a high correlation with those obtained from patch clamp techniques.

LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15121:

The LanthaScreen® TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).

How it works

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human ERR gamma ligand-binding domain (ERR gamma-LBD) that is tagged with glutathione-S-transferase (GST). To assay, ERR gamma-LBD is added to test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti- GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit contains ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

LanthaScreen™ TR-FRET PPAR gamma Coactivator Assay Kit, rabbit

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4548:

The LanthaScreen® TR-FRET PPAR gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR gamma ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a Peroxisome Proliferator Activated Receptor (PPAR) gamma ligand-binding domain (PPAR gamma-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format.

Agonist mode
To run the LanthaScreen® TR-FRET Peroxisome Proliferator Activated gamma Receptor Coactivator Assay in agonist mode (to identify agonist compounds), PPAR gamma-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After an incubation period at room temperature, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR gamma-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Antagonist mode
When the LanthaScreen® TR-FRET Peroxisome Proliferator Activated gamma Receptor Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR gamma-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluores-cein-coactivator peptide, and Tb-anti-GST antibody. The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode (Figure 2).

Contents and Storage:
The LanthaScreen® TR-FRET PPARγ Coactivator Assay Kit contains PPAR gamma-LBD (GST) protein, fluorescently labeled TRAP220/ DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Glucose Colorimetric Detection Kit (Invitrogen™)

The Glucose Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of glucose in serum, plasma, urine, buffers and tissue culture media.

This complete, ready-to-use kit includes clear 96-well plate(s), glucose standard, assay buffer, and other components to perform the assay. A 96-well microplate reader capable of reading optical density at 560 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, urine, buffers, and tissue culture media
• Sensitivity: 0.413 mg/dL
• Standard curve range: 0.5 mg/dL–32 mg/dL
• Reactivity: species independent

Background
Glucose is by far the most common carbohydrate. It is a monosaccharide, an aldose, a hexose, and a reducing sugar and is also known as dextrose, because it is dextrorotatory (rotates polarized light clockwise). The structure of glucose is shown below as both the straight chain and cyclic forms. For all biological and molecular events and for multiple cellular functions, energy is essential.

Energy is available in the form of ATP (adenosine triphosphate), most of which is generated through aerobic cellular respiration of carbohydrate and glucose, the major source of biological free energy in higher organisms. Reduced energy levels threaten cellular homeostasis and integrity. Impaired energy metabolism may trigger pro-apoptotic signaling (programmed cell death), oxidative damage, excitotoxicity, and impede mitochondrial DNA repair.

Assay principle
The Glucose Colorimetric Detection Kit is designed to quantitatively measure glucose in a variety of samples. A beta-D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the colorimetric substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room temperature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorimetric substrate to convert the colorless substrate into a pink-colored product. The pink product is read at 560 nm. Increasing levels of glucose cause a linear increase in color.

Related links
Learn more about ELISA kits
Learn more about other immunoassays

Vivid™ CYP2C9 Blue Screening Kit

Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid® CYP450 Screening Kits offer:
• Easy three-step procedure, "mix and read" format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well

Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP2C9 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® BOMCC Substrate is the substrate included in this particular kit. This substrate yields a product that emits a blue fluorescence.

Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.