Shop All Biochemical Assay Kits

LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15129:

The LanthaScreen® TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

How it works:
In the LanthaScreen® TR-FRET LXR beta Coactivator Assay, LXR beta-LBD is added to ligand test compounds followed by the addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET LXR beta Coactivator Assay Kit contains LXR beta-LBD (GST) protein, fluorescently labeled D22 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

PiPer™ Phosphate Assay Kit (Invitrogen™)

The PiPer Phosphate Assay Kit provides an ultrasensitive fluorometric method for detecting as little as 0.2 µM Pi in purified enzyme systems with a 100 µL assay volume.

Vivid™ CYP3A4 Red Screening Kit

Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid® CYP450 Screening Kits offer:
• Easy three-step procedure, "mix and read" format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well

Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A4 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® BOMR Substrate is the substrate included in this particular kit. This substrate yields a product that emits a red fluorescence.

Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

PiPer™ Pyrophosphate Assay Kit (Invitrogen™)

The PiPer™ Pyrophosphate Assay Kit provides a sensitive fluorogenic or chromogenic method for detecting as little as 0.4 µM (fluorescence) or 2 µM (absorbance) inorganic pyrophosphate in a 100 µL assay volume.

LanthaScreen™ TR-FRET PPAR alpha Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15133:

The LanthaScreen® TR-FRET PPAR (peroxisome proliferator activated receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR alpha ligand-binding domain (PPAR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

Agonist mode:
When running the LanthaScreen® TR-FRET PPAR alpha Coactivator Assay in agonist mode (to identify agonist compounds), PPAR alpha- LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).

Antagonist mode:
When the LanthaScreen® TR-FRET PPAR alpha Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR alpha-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluorescein-coactivator peptide, and Tb-labeled anti-GST antibody (Figure 2). The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode.

Contents and Storage:
The LanthaScreen® TR-FRET PPAR alpha Coactivator Assay Kit contains PPAR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or 4°C).

LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15121:

The LanthaScreen® TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).

How it works

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human ERR gamma ligand-binding domain (ERR gamma-LBD) that is tagged with glutathione-S-transferase (GST). To assay, ERR gamma-LBD is added to test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti- GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit contains ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Vivid™ CYP3A4 Green Screening Kit

Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid® CYP450 Screening Kits offer:
• Easy three-step procedure, "mix and read" format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well

Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A4 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® DBOMF Substrate is the substrate included in this particular kit. This substrate yields a product that emits a green fluorescence.

Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

LanthaScreen™ TR-FRET ER alpha Competitive Binding Kit

The LanthaScreen® TR-FRET ER alpha Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) alpha. The kit uses a human ER alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule ER ligand (Fluormone™ ES2) in a homogeneous mix-and-read assay format.

The Lanthascreen ER alpha Competitive Binding Assay is:

• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—TR-FRET assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs

Ferric Antioxidant Status Detection Kit (Invitrogen™)

The Ferric Antioxidant Status Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of Ferric Antioxidant Status (also referred as Ferric Reducing Antioxidant Power, FRAP) in serum, plasma, urine, teas, fruit juices, beer, cider, cell lysates, herbal and fruit extracts.

This complete, ready-to-use kit includes clear 96-well plate(s), ferrous chloride standard, ascorbic acid control, and other components to perform the assay. A 96-well microplate reader capable of reading optical density at 560 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, urine, teas, fruit juices, beer, cider, cell lysates, herbal and fruit extracts
• Sensitivity: 8.06 uM
• Standard curve range: 31.2 uM–1,000 uM
• Reactivity: species independent

Background
Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism. These free radicals (FR) are usually removed or converted into other products in vivo by an array of antioxidants. Organisms have developed complex antioxidant systems to protect themselves from oxidative stress, including the activity of specific enzymes (especially superoxide dismutase, glutathione peroxidase, catalase, glutathione reductase) as well as nonenzymatic compounds with antioxidant activity, such as beta-tocopherol, L-ascorbic acid, glutathione, coenzyme Q10, flavonoids, and albumin. The assay measures the antioxidant ability from all species.

Assay principle
The Ferric Antioxidant Status Detection kit is designed to quantitatively measure antioxidant status in a variety of samples. A ferrous chloride standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in assay buffer and added to the wells. The FRAP color solution is made by mixing reagent A and B with assay buffer. The FRAP color solution is added to all wells and the plate incubated at room temperature. Antioxidant power in the samples reacts with the FRAP color solution to generate a blue-colored product which is read at 560 nm.

Related links
Learn more about ELISA kits
Learn more about other immunoassays

Urea Nitrogen (BUN) Colorimetric Detection Kit (Invitrogen™)

The Urea Nitrogen (BUN) Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of urea nitrogen in serum, plasma, urine, saliva and tissue culture media samples.

This complete, ready-to-use kit includes clear 96-well plate(s), urea nitrogen standard, and other components to perform the assay. A 96-well microplate reader capable of reading optical absorption at 450 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, urine, saliva, and tissue culture media samples
• Sensitivity: 0.030 mg/dL
• Standard curve range: 0.156 mg/dL–10 mg/dL
• Reactivity: species independent

Background
Urea is a by-product of protein metabolism by the liver, and is therefore removed from the blood by the kidneys. Urea freely filters through the glomerulous, but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate, the greater amount of urea nitrogen is cleared from circulation and eliminated through the kidneys. Urea nitrogen is identical across all species and this kit will measure urea nitrogen from sources other than human.

Assay principle
The Urea Nitrogen ((also called BUN) Colorimetric Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. A urea nitrogen standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with color reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm.

Related links
Learn more about ELISA kits
Learn more about other immunoassays

LanthaScreen™ TR-FRET PPAR delta Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15134:

The LanthaScreen® TR-FRET PPAR (peroxisome proliferator activated receptor) delta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR delta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR delta ligand-binding domain (PPAR delta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

When running the LanthaScreen® TR-FRET PPAR delta Coactivator Assay in agonist mode (to identify agonist compounds), PPAR delta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-labeled coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the TR-FRET ratio of 520/495 is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR delta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET PPAR delta Coactivator Assay Kit contains PPAR delta-LBD (GST) protein, fluorescently labeled C33 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C or 4°C).

LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15122:

The LanthaScreen® TR-FRET Retinoic Acid Receptor (RAR) alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR alpha ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

How it works

The LanthaScreen® TR-FRET RAR alpha Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RAR alpha ligand-binding domain (RAR alpha-LBD) that is tagged with glutathione-S-transferase (GST). To assay, RAR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen® TR-FRET RAR alpha Coactivator Assay Kit contains RAR alpha-LBD (GST) protein, fluorescein-labeled D22 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Vivid™ CYP3A4 Blue Screening Kit

Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid® CYP450 Screening Kits offer:
• Easy three-step procedure, "mix and read" format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well

Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A4 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® BOMCC Substrate is the substrate included in this particular kit. This substrate yields a product that emits a blue fluorescence.

Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Vivid™ CYP1A2 Blue Screening Kit

Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid® CYP450 Screening Kits offer:
• Easy three-step procedure, "mix and read" format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well

Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP1A2 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® EOMCC Substrate is the substrate included in this particular kit. This substrate yields a product that emits a blue fluorescence.

Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15141:

The LanthaScreen® TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand’s EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).