Shop All Phosphate & Pyrophosphate Assay Kits

EnzChek™ Phosphate Assay Kit (Invitrogen™)

The EnzChek® Phosphate Assay Kit provides a fast and sensitive spectrophotometric method for the quantification of inorganic phosphate in solution and for phosphate released during ATPase or GTPase enzymatic reactions.

See our complete line of Fluorescence Microplate assays.

• Sensitivity of 2 to 150 µM phosphate
• Spectrophotometric microplate-based assay detects a change in absorbance maximum from 330 nm to 360 nm
• Continuous monitoring of phosphate generated by ATPase or GTPase activity

In the presence of inorganic phosphate, the 2-amino-6-mercapto-7-methylpurine riboside (MESG) substrate is converted by the purine nucleoside phosphorylase (PNP) enzyme to the ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine product. This enzymatic conversion of MESG results in a spectrophotometric shift in maximum absorbance from 330 nm for the substrate to 360 nm for the product.

Sensitivity of the assay is in the range of 2 to 150 µM inorganic phosphate (2 to 150 nanomoles phosphate in a 1 mL volume), and the reaction can be performed over a pH range of 6.5 to 8.5. The EnzChek® phosphate reaction is sufficiently fast and quantitative for use in stopped-flow kinetic experiments.

EnzChek™ Pyrophosphate Assay Kit (Invitrogen™)

The EnzChek® Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In this assay the formation of a chromophoric product is detected spectrophotometrically.

See our complete line of Fluorescence Microplate assays.

• Sensitivity limit of 1 µM pyrophosphate (~0.2 µg/mL)
• Acommodates a pH range of 6.5 to 8.5

The EnzChek® Pyrophosphate Detection Kit provides a fast, convenient, and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase, and the enzymatic activation of fatty acids to form their coenzyme A esters.

The EnzChek® Pyrophosphate Assay Kit is a modification of our EnzChek® Phosphate Assay Kit (Cat. No. E6646). In the presence of inorganic phosphate, the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted by the purine nucleoside phosphorylase (PNP) enzyme to ribose 1-phosphate and 2-amino-6-merc apto-7-methylpurine. The enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm to 360 nm. The EnzChek® Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of pyrophosphate into two equivalents of phosphate. The phosphate is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of pyrophosphate into two molecules of phosphate.

PiPer™ Pyrophosphate Assay Kit (Invitrogen™)

The PiPer™ Pyrophosphate Assay Kit provides a sensitive fluorogenic or chromogenic method for detecting as little as 0.4 µM (fluorescence) or 2 µM (absorbance) inorganic pyrophosphate in a 100 µL assay volume.

PiPer™ Phosphate Assay Kit (Invitrogen™)

The PiPer Phosphate Assay Kit provides an ultrasensitive fluorometric method for detecting as little as 0.2 µM Pi in purified enzyme systems with a 100 µL assay volume.