Shop All Enzyme Detection & Activity Assays

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S⁄T-X-X, where Hyd is bulky hydrophobic, F > L > V>>A, and X is any residue.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 5 Peptide

A coumarin and fluorescein-labeled serine⁄threonine peptide substrate which can be used for kinase screening in an antibody-free format.

Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells Invitrogen™

The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

• Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range.
• Assay sensitivity is 100 to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Ideal for Screening
This homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen® system uses Galacton-Star® chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

For Research Use Only. Not for use in diagnostics procedures.

Pierce™ Gaussia Luciferase Glow Assay Kit Thermo Scientific™

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour), especially in the presence of Gaussia-Dura luciferase reporter.

Features of the Gaussia Luciferase Glow Assay Kit:

Sensitive—highly sensitive detection of Gaussia luciferase activity
Stable—increased signal stability
Simple—does not require a luminometer with injectors
Compatible—assay reagents compatible with other Gaussia luciferases
Automation-friendly—amenable to high throughput assays
Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
Safe—allows one to perform non-radioactive assays

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Gaussia luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia-Dura Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Gaussia luciferases or derivatives that use coelenterazine as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions.

The Gaussia-Dura gene used in the Thermo Scientific Gaussia-Dura Luc Vectors is derived from the wild type Gaussia gene and provides equally bright but more stable light output. The Gaussia Glow Assay Kit reagent yields a Gaussia-Dura luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 6 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains an R-X-R-X-X-S-Hyd consensus sequence, where Hyd is a bulky hydrophobic residue, and X is any residue.

EnzChek™ Phosphatase Assay Kit Invitrogen™

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes' patented 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate (D6567). DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ~1000 assays, using a reaction volume of 100 µL per assay. Fluorescence (excitation/emission maxima ~358/455 nm) can be measured in a fluorescence microplate reader or a standard fluorometer.

Z'-LYTE™ Kinase Assay Kit - Tyrosine 1 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for SRC.

Pierce™ Fluorescent Protease Assay Kit Thermo Scientific™

The Thermo Scientific Pierce Fluorescent Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples.

This kit includes fluorescein-labeled casein as a substrate for assessing protease activity in a sample by either fluorescence resonance energy transfer (FRET) with a standard fluorometer or fluorescence polarization (FP) with capable instrumentation. FTC-Casein is native casein that has been labeled using a large molar excess of fluorescein isothiocyanate (FITC). Fluorescence properties of this heavily-labeled, intact protein substrate change dramatically upon digestion by proteases, resulting in a measurable indication of proteolysis.

Features of the Fluorescent Protease Assay Kit:

Casein substrate—measure activity of any protease that cleaves casein into peptide fragments
Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference
Sensitive—1000 times more sensitive than assays that use unmodified forms of casein
Simple and fast—test tube and microplate protocols are completed in less than one hour
Homogenous—addition steps only; no separation, transfer or stop steps required
Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interest

Applications:
• Assess functional integrity and overall progress of protease purification
• Quantify protease contamination in protein samples
• Characterize the activity of unusual proteases compared to trypsin
• Evaluate buffer conditions for their affects on protease function and activity

Additional Specifications:
• Substrate: fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267
• Basis of assay: change in fluorescence resonance (FRET) or fluorescence polarization (FP)
• Measurement: instrument with fluorescein excitation and emission filters (485/538nm)

Related Products
Pierce™ Colorimetric Protease Assay Kit
FITC-Casein for Pierce™ Fluorescent Protease Assay Kit

Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit Thermo Scientific™

The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

Features of the Cypridina-Firefly Luciferase Dual Assay Kit:

Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
Sensitive—measure bright blue Cypridina and red firefly luciferase activities in the same sample
Fast—no quenching step required, unlike in traditional sequential dual assays
Multiplex—capable of quantitating two cellular activities in the same sample(s)

The Pierce Cypridina-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animated videos below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Includes:
Cell lysis buffer, reaction buffer, and substrates Vargulin and D-luciferin

Requires:
Cypridina and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Cypridina Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Applications:
• Study two regulatory elements at the same time
• Monitor two signaling pathways simultaneously
• Enable studying more than one target per screen (e.g. off-target effects)

Application Videos:
Cypridina luciferase produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent signal (λmax=463nm) produced by Cypridina luciferase results from the oxidation of Vargulin. This reaction does not require adenosine triphosphate (ATP) or other cofactors. Red firefly luciferase is a mutant form of the Japanese firefly luciferase from Luciola cruciata. This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP. Red firefly and Cypridina luciferases are used in the dual assay because the signals are spectrally resolvable.

More Product Data
Highly sensitive multiplex luciferase reporter assays
Simultaneous dual-emission detection of luciferase reporter assays
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 7 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains a PKC consensus sequence (R⁄K)1-3(X)0-2 (S⁄T) (X)0-2(R⁄K)1-3, where X is any residue.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 21 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This substrate is based upon CDC25C, containing Ser-216.

EnzChek™ Gelatinase/Collagenase Assay Kit, 250-2,000 assays Invitrogen™

The EnzChek Gelatinase/Collagenase Assay Kit provides a sensitive, convenient and fast fluorometric method for measuring gelatinase or collagenase activity in purified enzyme systems, cell/tissue lysates or for screening inhibitors in a high-throughput format. The substrate in the EnzChek kit is our fluorescein-labeled DQ gelatin conjugate that is highly labeled so that the fluorescence signal is quenched until enzymatic digestion yields highly fluorescent fragments. This substrate is also available separate from the kit (D-12054).

Z'-LYTE™ Kinase Assay Kit - Tyrosine 2 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for ABL.

Phospha-Light™ SEAP Reporter Gene Assay System Invitrogen™

Phospha-Light™ assay system is a chemiluminescent reporter gene assay for the sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.

• Detection of a secreted reporter permits analysis without destroying valuable cells.
• Wide dynamic range of alkaline phosphatase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for b-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without the need to perform numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Monitor Expression Over Time
Phospha-Light™ assay system allows sensitive detection of secreted alkaline phosphatase (SEAP) reporter protein in cell culture media. The SEAP gene product is a truncated form of human placental alkaline phosphatase. A secreted reporter protein eliminates the need to prepare cell extracts. A population of cells can be monitored over time and remains intact for further experimentation.

Proprietary Reagents for Ultra-Sensitive Detection
Phospha-Light™ assay system is one of the easiest and fastest methods for optimizing transfection efficiency. A combination of proprietary reagents is the key to the highly sensitive detection achievable with the Phospha-Light™ assay. The system consists of CSPD® high-performance alkaline phosphatase substrate, Emerald™ luminescence enhancer, and a unique buffer system designed to specifically inhibit non-placental endogenous alkaline phosphatase activity. The persistent glow of the chemiluminescent signal permits the use of luminometers without the need for injectors for measurement.

Applications
Phospha-Light™ reporter gene assay system has been used widely for reporter gene assays to measure gene expression in established cell lines and in transfected primary cells, including as a gene knockdown⁄RNA interference read-out. Phospha-Light reporter gene assay has been used for a wide variety of viral functional assays, including viral gene expression assays, viral replication, viral fusogenicity, virus neutralization and viral-mediated cell-cell fusion, and viral infectivity. Use of the SEAP reporter protein is very enabling for in vivo reporter gene assays, by assaying serum samples from transgenic, transfected or viral vector-infected animals. Phospha-Light reporter gene assay system has been used to measure SEAP levels in sera from transgenic or transfected whole animals, including mouse, rat, marmoset, monkey and pig sera, and in chicken egg allantoic fluid. The mouse SEAP protein (mSEAP) has recently been developed for improved SEAP protein stability in transgenic mice, and the Phospha-Light™ system has been used for sensitive detection of mSEAP. For these and additional references, please see our bibliography in the Literature⁄Resources section.

For Research Use Only. Not for use in diagnostics procedures.

FITC-Casein for Pierce™ Fluorescent Protease Assay Kit Thermo Scientific™

FTC-Casein, 2.5 mg, lyophilized, FRET/FP Certified Grade, for use with the Pierce Fluorescent Quantitative Protease Assay Kit.

Related Products
Pierce™ Colorimetric Protease Assay Kit
Pierce™ Fluorescent Protease Assay Kit
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