Shop All DNA⁄RNA Quantitation Assay Kits

Quant-iT™ OliGreen™ ssDNA Assay Kit (Invitrogen™)

Our Quant-iT™ OliGreen® ssDNA Assay Kit contains an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. In addition to the dye, the kit also includes buffer and an oligonucleotide standard. The OliGreen® reagent is also available as a stand alone product ([PRODUCT O7582]).

Qubit™ dsDNA BR Assay Kit (Invitrogen™)

The Qubit dsDNA BR Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for dsDNA BR (Broad Range) quantitation?
• For 1–20 samples: use this Qubit dsDNA BR Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA BR Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ RNA XR Assay Kit (Invitrogen™)

The Qubit RNA XR (Extended Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein, are well-tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations from 1 ng/µL to 8 µg/µL, providing an assay range from 20 ng –8 µg. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add the sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using a Qubit 2.0, 3, or 4 fluorometer.

Notes:
1. The Qubit RNA XR Assay works on Qubit 2.0, 3, and 4 fluorometers. It will not work on the original Qubit 1.0 Fluorometer. Download the MyQubit .qbt file for the Qubit 2.0 or 3 fluorometer.
2. The Qubit RNA XR Assay Kit replaces the Qubit RNA BR (broad-range) Assay Kit.
3. 500-mL thin-walled PCR tubes (Cat. No. Q32856) are required but not included.

Qubit™ microRNA Assay Kit (Invitrogen™)

The Qubit microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA) using the Qubit Fluorometer, even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng in the assay tube following the supplied protocol below, even in the presence of mRNA.

Features of Qubit microRNA assay:
• Accurately detects as little as 0.5 ng miRNA in the assay tube
• Core dynamic range of 5–500 ng/mL
• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 µg/mL.

Which product to choose for microRNA quantitation?

• For 1–20 samples: use this Qubit microRNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT microRNA Assay Kit with microplate reader
• For >2000 samples: use the Quant-iT microRNA Reagent with microplate reader

Notes:
1. The Qubit microRNA assay file is included on all Qubit 3 and Qubit 4 fluorometers, but may require installation onto the Qubit 2.0 Fluorometer. Uploading new assays to the Qubit 2.0 Fluorometer requires version 3.10 or later of the Qubit firmware.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ dsDNA HS Assay Kit (Invitrogen™)

The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for dsDNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA HS Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.
3. Also available as a "1X" dsDNA assay kit that provides reagent and buffer in a formulation that is stable as a ready-to-use solution for up to one year after preparation.

Quant-iT™ microRNA Assay Kit (Invitrogen™)

The Quant-iT™ microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA), even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng following the supplied protocol, even in the presence of mRNA.

Features of Quant-iT™ microRNA Assay Kit:

• Accurately detects as little as 0.5 ng miRNA in a microplate well
• Core dynamic range of 5–500 ng/mL
• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL

The Quant-iT™ microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL of sample, mix, then read the fluorescence. The assay is performed at room temperature, and the signal is stable for approximately 3 hours. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 µg/mL.

Which product to choose for microRNA Quantitation?

• For 1–20 samples: use the Qubit® microRNA Assay Kit with the Qubit® Fluorometer
• For 20–2,000 samples: use this Quant-iT™ microRNA Assay Kit with microplate reader
• For >2,000 samples: use the Quant-iT™ microRNA Reagent with microplate reader

Discover the Qubit® Fluorometer
The Qubit® 2.0 Fluorometer is Life Technologies’ second generation fluorometer designed to work seamlessly with our highly specific and sensitive Qubit® quantitation assays as well as the assays you create with the new MyQubit feature.

Introducing MyQubit
New firmware called "MyQubit" allows you to create applications in minutes using parameters that can easily be uploaded to the instrument using a USB drive, without changing the existing assays. All new Qubit® 2.0 instruments are pre-loaded with the new MyQubit firmware, and existing owners can download the firmware here: V3.10 firmware download. Any reagent or assay that is spectrally compatible with the Qubit® hardware can be adapted for use with the Qubit® 2.0 Fluorometer. Additionally we have developed assays that are ready to download, including glucose and cholesterol assays for research and drug discovery applications.

Qubit™ 4 System Verification Assay Kit (Invitrogen™)

The Qubit 4 System Verification Assay Kit is a fast, easy-to-use, reagent-based assay that tests the performance of the Qubit 4 Fluorometer. The kit consists of three components: a blank reagent solution, a green fluorescent reagent, and a far-red fluorescent reagent. The green fluorescent reagent is designed to test the blue excitation (470 nm LED; 430-495 nm excitation filter) and green fluorescence detection (510-580 nm emission filter) on the fluorometer, while the far-red fluorescent reagent is designed to test the red excitation (635 nm LED; 600-645 nm excitation filter) and far-red fluorescence detection (665-720 nm emission filter).

The assay kit provides sufficient reagents to perform up to 50 assays.

Note: The Qubit 4 System Verification Assay Kit can only be used with the Qubit 4 Fluorometer.

Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™)

Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.

PicoGreen® dsDNA Quantitation Reagents are:

• Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1)
• Specific for dsDNA in the presence of equimolar amounts of RNA (Figure 2)
• Easy to use-just add the dye to sample, wait 5 minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers and Fluorescence

Applications: The PicoGreen® dsDNA Quantitation Reagent and Kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.

Quant-iT™ RiboGreen™ RNA Assay Kit (Invitrogen™)

The Quant-iT™ RiboGreen® RNA Assay Kit contains Quant-iT™ RiboGreen® RNA reagent as well as buffers and RNA standards. Quant-iT™ RiboGreen® RNA reagent is one of the most sensitive detection dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA.

Check out our products for nucleic acid purification

Qubit™ RNA HS Assay Kit (Invitrogen™)

The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer.

Which product to choose for RNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit RNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT RNA HS Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Qubit™ 1X dsDNA HS Assay Kit (Invitrogen™)

The Qubit 1X dsDNA HS (High-Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The "1X" dsDNA assay kit provides reagent and buffer in a formulation that is stable as a ready-to-use solution for up to one year after preparation. Simply add your sample (any volume between 1 µL and 20 µL is acceptable) and read the concentration using a Qubit 2.0, 3, or 4 fluorometer. Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well-tolerated in the assay.

Notes:
1. The Qubit 1X dsDNA HS Assay works on Qubit 2.0, 3, and 4 fluorometers. It will not work on the original Qubit 1.0 Fluorometer. Download the MyQubit .qbt file for the Qubit 2.0 or 3 fluorometer.
2. 500-µL thin-walled PCR tubes (Cat. No. Q32856) are required but not included.

Qubit™ RNA BR Assay Kit (Invitrogen™)

The Qubit RNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations from 1 ng/µL to 1 µg/µL providing an assay range from 20–1,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer.

Which product to choose for RNA BR (Broad Range) quantitation?
• For 1–20 samples: use this Qubit RNA BR Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT RNA BR Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Quant-iT™ RNA XR Assay Kit (Invitrogen™)

The Quant-iT RNA XR (Extended Range) Assay Kit provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides, unlike Quant-iT RiboGreen RNA Reagent or RiboGreen reagent-based assay kits. Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein, are well-tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations from 1 ng/µL to 8 µg/µL, providing an assay range from 20 ng –8 µg. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add the sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using a fluorescent-based microplate reader.

Note: The Quant-iT RNA XR Assay Kit replaces the Qubit RNA BR (broad-range) Assay Kit.

Quant-iT™ RNA HS Reagent (Invitrogen™)

The Quant-iT™ RNA HS Reagent makes RNA quantitation easy and accurate, with a linear detection range of 5–100 ng in sample volumes of 1–20 µl and selectivity for RNA, even in the presence of an equal mass of DNA. Using the protocol provided, this concentrated assay reagent provides sufficient material to perform at least 2000 assays in a 96-well microplate format. The assay is performed at room temperature, and the signal is stable for approximately 3 hours. Common contaminants, such as salts, solvents, detergents, and protein are well tolerated in the assay.

Important Features of Quant-iT™ RNA HS Reagent:

• Accurately detects as little as 2 ng RNA in a microplate well
• Core dynamic range of 5–100 ng
• Approximate fluorescence excitation/emission maxima of 644/673 nm

Additional Options for RNA Quantitation:

• For 1–20 samples: use Qubit® RNA Assay Kit with the Qubit® Fluorometer
• For 20–2,000 samples: use Quant-iT™ RNA Assay Kit with fluorescent microplate readers

Discover the Qubit® Fluorometer
The Qubit® 2.0 Fluorometer is Life Technologies’ second generation fluorometer designed to work seamlessly with our highly specific and sensitive Qubit® quantitation assays as well as the assays you create with the new MyQubit feature.

Introducing MyQubit
New firmware called "MyQubit" allows you to create applications in minutes using parameters that can easily be uploaded to the instrument using a USB drive, without changing the existing assays. All new Qubit® 2.0 instruments are pre-loaded with the new MyQubit firmware, and existing owners can download the firmware here: V3.10 firmware download. Any reagent or assay that is spectrally compatible with the Qubit® hardware can be adapted for use with the Qubit® 2.0 Fluorometer. Additionally we have developed assays that are ready to download, including glucose and cholesterol assays for research and drug discovery applications.

Qubit™ 1X dsDNA HS Assay Lambda Standard (Invitrogen™)

The Qubit dsDNA HS (High-Sensitivity) Assay Lambda Standard can be used with either the Qubit 1X dsDNA HS Assay Kit or the Qubit dsDNA HS Assay Kit. Sufficient material is supplied to perform standards with up to 500 samples.

Note: 500-mL thin-walled PCR tubes (Cat. No. Q32856) are required for all Qubit assays and are not included with this Qubit assay standard.