Shop All DNA⁄RNA Purification Kits & Reagents

Magnetic Bead Purification Module (Ion Torrent™)

The Magnetic Bead Purification Module is a cleanup kit used in the construction of RNA libraries for sequencing on the Ion Torrent PGM™sequencer. This module is included in the Ion Total RNA-Seq Kit v2, but may also be purchased separately if additional modules are needed.

For small RNA library construction using the Ion Total RNA-Seq Kit v2, the module is used for cleanup after cDNA synthesis, PCR amplification, and for the small RNA enrichment steps. This magnetic bead-based cDNA cleanup step replaces the gel size selection step in the previous version of the kit. For whole transcriptome library construction using the Ion Total RNA-Seq Kit v2, the module is used for cleanup after the fragmentation, cDNA, and PCR amplification steps.

This module is not for use alone or in conjuction with any kits other than the Ion Total RNA-Seq Kit v2.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

MagMAX™ Microbiome Lysis Solution (Applied Biosystems™)

This lysis buffer is included in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42357, and A42358). It is made available separately for applications that require more lysis solution than is provided in the kit.

Agarase (0.5 U/µL) (Thermo Scientific™)

Thermo Scientific Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides. Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amplification, cloning, sequencing, etc.

MagMAX™-96 DNA Multi-Sample Kit (Invitrogen™)

MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automated format processing.

The demands for DNA yield and purity have become more stringent with the development of new analytical methods and technologies, such as high-throughput genotyping, and next-generation DNA sequencing platforms. MagMAX™ DNA Multi-Sample Kits meet the challenge by delivering highly pure DNA that is free of nucleases, proteins, and other inhibitors of downstream enzymatic reactions.
As shown in Table 1, DNA with high yield and purity can be obtained from a variety of materials using the MagMAX™ DNA Multi-Sample Kits. This is in contrast to filter membrane–based methods that often require different kit configurations for various input materials. For applications requiring maximal yields, for small batch sizes, or for DNA archival purposes MagMAX™ DNA Multi-Sample Kit (tube format, manual processing) may be desirable because larger sample inputs are accommodated. DNA yields suitable for most routine applications may be obtained using the MagMAX™-96 DNA Multi-Sample Kits (96-well plate format, compatible with automated handling) (Table 1).

The MagMAX™ DNA Multi-Sample Kit also delivers high yields of DNA from lipid or glycogen rich samples, such as brain and liver tissues, respectively. Figure 1 shows more than twice as much DNA was purified from tissues with the MagMAX™ DNA Multi-Sample Kit than with the leading competitor’s filter membrane–based method.

Most enzymatic molecular applications are prone to inhibition by analytes carried over from the sample matrix (e.g., heme, heparin, salts, protein) or the purification method itself (phenol, salts, alcohol). Figure 2 illustrates the effect of DNA purity on sensitive molecular applications, such as quantitative PCR (qPCR). DNA was extracted from blood using the MagMAX™-96 DNA Multi-Sample Kit, or a competitor’s filter membrane–based kit and subjected to real-time PCR analysis. The cycle threshold CT values obtained with the DNA purified using the MagMAX™ DNA Multi-Sample Kit were lower than those obtained with the filter membrane kit–purified DNA, indicating the MagMAX™ kit yields higher-purity DNA.

MagMAX™ DNA Multi-Sample Kits provide purified DNA that is suitable for a variety of Applied Biosystems application workflows (Figure 3), including SNP genotyping and DNA sequencing analysis (see Figure 4). To see the full performance description of DNA purified using the MagMAX™ DNA Multi-Sample Kits in TaqMan® SNP genotyping assays and with BigDye® chemistry in DNA sequence analysis, view the MagMAX™ DNA Multi-Sample Kit Product Bulletin.

MagMAX™ Plant RNA Isolation Kit (Applied Biosystems™)

The MagMAX Plant RNA Isolation Kit is designed for automated high-throughput or manual purification of total RNA from wide variety of plant species and tissue types. It utilizes paramagnetic bead technology, enabling high yields and robust performance. The high binding capacity, uniform particle size, and rapid magnetic response of MagMAX magnetic beads makes the technology ideal for high-throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low- throughput users. The resulting high quality purified RNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, and other enzymatic reactions.

Versatile—suitable for any plant type and part, including woody, polyphenol-rich, and lignified samples
High yields of pure total RNA
High quality—isolates intact total RNA, free of inhibitors and gDNA contamination. Isolated RNA is suitable for long term storage at -20°C.
Flexible—easily adjustable for high-throughput automation platforms and manual processing

Applications
The MagMAX Plant RNA Isolation Kit is designed for fast extraction of high purity RNA suitable for all conventional molecular biology applications, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher Duo Prime or KingFisher Flex processors.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps.

Optimized bead surface chemistry
The Dynabeads MyOne Carboxylic Acid utilized in the MagMAX Plant RNA Isolation Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads magnetic technology ideal for the recovery of the RNA targets found in a variety of plant samples. Once captured, the RNA is then eluted in 100 µL and ready for downstream applications.

MagMAX™ Viral/Pathogen Binding Beads (Applied Biosystems™)

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). They are made available separately for applications that require more binding beads than are provided in the kit.

PrepSEQ™ Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating (Applied Biosystems™)

The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating was developed for the AFNOR-approved MicroSEQ® Listeria species and Listeria monocytogenes Real-Time PCR detection workflows. A spin, transfer, and bead beating protocol efficiently lyses Listeria from a 750 µL aliquot of more challenging enriched food samples (e.g., samples containing high fat or otherwise prone to PCR inhibition). The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating meets the needs of lower throughput food laboratories.

NOTE: Standard samples that do not contain high fat or demonstrate PCR inhibition should be processed using the PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput (up to 100 samples a day)
• Inactivates PCR inhibitors and produces high quality DNA for downstream detection
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from the most challenging sample types.

Designed for Laboratories that Prepare up to 100 Samples a Day
A spin column-based preparation enables small batch sizes to be managed while requiring only simple benchtop devices to process, thereby limiting the capital expenditure associated with much higher throughput automation devices.

Removes PCR Inhibitors and Produces High Quality DNA
The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating offers a simple, cost-effective way to prepare Listeria DNA from more difficult starting matrices such as cheese, milk, and high fat meats. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube. The pellet is resuspended, then transferred (to avoid carryover, which inhibits PCR) to a new tube containing zirconium beads for mechanical lysis.

MagMAX™-96 Viral RNA Isolation Kit (Applied Biosystems™)

The MagMAX-96 Viral RNA Isolation Kit is for the isolation of viral RNA and DNA from diverse sample types (e.g., environmental samples and biofluids). Nucleic acids purified using this kit are ideal for PCR and RT-PCR applications. For a NVSL-validated version of this kit, please see the MagMAX-96 AI⁄ND Viral RNA Isolation Kit.

Benefits of MagMAX magnetic bead-based purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Optimized for isolation of virus from biofluids
The MagMAX Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity. Without the need for organic solvents or RNA precipitation, the MagMAX Viral RNA Isolation Kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields. With this kit, DNA and RNA input levels ranging from 20 copies to 25 x 106 copies have been quantitatively recovered from plasma, serum, and milk. And even with less sample input, pathogen detection was as sensitive (equivalent CTs) or more sensitive (lower CTs) using nucleic acid isolated with the MagMAX kit when compared to nucleic acid isolated using another company's product.

Accessory Products:
This kit is optimized for automated nucleic acid purification using KingFisher magnetic particle processors. This kit can also be used for manual nucleic acid purification using a 96-well magnetic ring stand. The Magnetic Stand-96 (Cat. No. AM10027) and the 96-well Magnetic Ring Stand (Cat. No. AM10050) are designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates. The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors.

iPrep™ ChargeSwitch™ Forensic Kit (Invitrogen™)

• Flexibility in starting sample type, for quick and easy processing of different samples simultaneously
• High sensitivity with samples containing very low amounts of DNA
• Fast automated extraction time of 20 minutes
• Standardized procedures minimize human error; preset protocols and prefilled cartridges eliminate variation

The iPrep ChargeSwitch® Forensic Kit offers a single protocol for efficiently isolating DNA from all kinds of forensic samples (Table 1), even if
the DNA copy number is low or the DNA is partially degraded.

Designed specifically for use with the iPrep™ Purification Instrument, the iPrep™ ChargeSwitch® Forensic Kit takes advantage of the powerful ChargeSwitch® technology, a major advance in DNA purification. This technology is based on a unique, ionizable nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium (Figure 1). Purification is achieved through a simple three-step procedure in aqueous buffers, and avoids the use of guanidine, ethanol, and other troublesome reagents.

Contents and Storage:
The iPrep™ ChargeSwitch® Forensic Kit provides all reagents (supplied in prefilled cartridges), tubes, and tips needed for isolating DNA from forensic cell samples. Reagent cartridges can be stored at room temperature; store lysis buffer and proteinase K at 4°C.

Mouse RiboPure™-Blood RNA Isolation Kit (Invitrogen™)

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion® kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNAlater®, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater® Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 µg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 µg from 0.5 mL blood.

PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing (Applied Biosystems™)

The PrepSEQ® Nucleic Acid Extraction Kit produces high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples. The quantity and quality of the DNA can greatly impact the success of the sample analysis process and the overall quality of the final result. Food and environmental samples can be challenging to process because they often contain PCR inhibitors. For laboratories looking to achieve better real-time PCR results and improve overall efficiency, the PrepSEQ™ Nucleic Acid Extraction Kit meets these needs.

Automated Sample Preparation for PCR-Based Assays
• Extract high-quality bacterial DNA
• Process a wide range of food and environmental samples
• Fast time-to-results
• Prepare hundreds of samples per shift per instrument

High-Throughput Food and Environmental Sample Preparation
PCR-based bacterial assays have become an important tool in food safety and environmental testing. The Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit prepares high-quality bacterial DNA from food and environmental samples for PCR-based testing using magnetic bead-based extraction. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Streamlined Workflow
An easy-to-use, easy-to-learn protocol simplifies integration of the PrepSEQ™ Nucleic Acid Extraction Kit into the laboratory workflow. The same protocol can be used on a wide range of sample types, and the MagMAX™ Express-96 instrument automates many of the workflow steps to reduce hands-on time.

A Complete Solution
The PrepSEQ™ Nucleic Acid Extraction Kit is part of a fully integrated, start-to-finish solution for food and environmental testing. The kit has been performance-verified for food and environmental testing with MicroSEQ® Microbial Detection Kits, the Applied Biosystems® 7500 Fast Real-Time PCR System, and RapidFinder™ Express Software. For research use only. Not intended for any animal or human therapeutic or diagnostic use.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

MagMAX™ Viral/Pathogen Proteinase K (Applied Biosystems™)

This Proteinase K is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more Proteinase K than is provided in the kit.

MagMAX™-96 Blood RNA Isolation Kit (Invitrogen™)

This Ambion® kit is for the rapid, high-throughput purification of pathogen RNA from mammalian whole blood, milk, and other biofluids in 96-well plates. RNA purified using the kit is ideal for RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions.

• Easily isolate viral or total RNA from whole blood
• Save precious time—complete the protocol in less than 1 hour
• Walk away—integrate with established robotic platforms
• Validated with human, bovine, porcine, and ovine whole blood

Benefits of MagMAX™ Magnetic Bead-Based Purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high- efficiency binding, the bound RNA can be eluted in just 30–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Isolate RNA from as Little as 50 µl of Blood:
For the highest-quality RNA, use whole blood samples that have not been frozen. Approximately 300–500 ng of intact RNA can be consistently purified from 50 µl of human whole blood. Bovine, porcine, and ovine whole blood samples can also be used. Yields vary from species to species (see data). The recovered RNA is devoid of PCR inhibitors such as proteins and heme, commonly found in RNA isolated from blood.

Isolate Viral and Total RNA from Whole Blood Samples:
The MagMAX™-96 Blood RNA Isolation Kit eliminates sample preprocessing, which can cause sample mix-up and cross-contamination. The procedure is fast (<1 hour), simple, and well suited for automation. The kit can also be used to process fewer than 96 samples at one time and is compatible with either manual processing using multi-channel pipettors or processing with robotic liquid handlers. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource. The MagMAX™-96 Blood RNA Isolation Kit can also be used for total nucleic acid isolation by omitting the DNase treatment.

Accessory Products:
Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the kit that may also be purchased separately. A 96-well magnetic ring stand is required to use this kit. The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96-well U-bottom microplates. The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors. For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended.

ChargeSwitch™ gDNA Mini Tissue Kit (Invitrogen™)

The ChargeSwitch® gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance

High Quality, High Yield Plasmid DNA
The ChargeSwitch® gDNA Mini Tissue Kit allows purification of genomic DNA from 10-25 mg quantities of tissue typically in less than 15 minutes following sample preparation and lysis. ChargeSwitch® gDNA Mini Tissue Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 30 µg of gDNA from 0.5 cm mouse tail tips or approximately 25 mg of tissue. For spleen tissue, reduce tissue size to 10 mg.

Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch® gDNA Mini Tissue Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy "on the magnet" and "off the magnet" sample processing.

Improved Performance in Demanding Downstream Applications
The ChargeSwitch® gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.

For Research Use Only. Not intended for diagnostic procedures.

PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™)

The PureLink® HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include:

Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
High-purity, low-endotoxin product—pure enough for mammalian cell transfection
Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification
The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.

Contaminant-free DNA
Unlike cesium chloride gradient protocols, PureLink® HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink® HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.1–1 EU/µg, making this plasmid DNA ideal for mammalian cell transfection.