Shop All DNA⁄RNA Purification Kits & Reagents

GeneCatcher™ gDNA Automated Blood Kit (Invitrogen™)

The GeneCatcher™ gDNA Blood Kits allow rapid and efficient extraction of genomic DNA (gDNA) from human blood, including archived or poorly stored blood samples. Genomic DNA is extracted from blood samples using the cost-effective, user-friendly magnetic bead based GeneCatcher™ Technology without the use of centrifugation (Figures 1,2).

High purity DNA for downstream processing DNA purified using GeneCatcher™ Technology provides a purity suited to highly reliable downstream processing. GeneCatcher™ purified DNA has been validated in many applications, including restriction endonuclease digestion (Figure 3), second generation multiplex and single tandem repeat (SGM+STR) profiling (Figure 4) and quantitative PCR (Figure 5).

GeneCatcher™ protocols - ready for automationGeneCatcher™ Technology has been optimized and designed for scalable and automated gDNA isolation from large blood samples using the Tecan Freedom EVO™ liquid handler (Figure 6), featuring:

• Fully scalable chemistry in batches of 8 (40 samples 3-10 ml blood samples per 8 hour day)
• High purity and yield of extracted genomic DNA yields between 300 µg - 500 µg per 10 ml sample

Lower elution volumes, resulting in concentrated DNA ideal for archiving in a standard microcentrifugetube

RiboMinus™ Transcriptome Isolation Kit, human/mouse (Invitrogen™)

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods.

The RiboMinus™ Transcriptome Isolation Kits enable:

Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

RiboPure™ RNA Purification Kit, yeast (Invitrogen™)

For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Ambion® kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.

• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents

It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).

Accessory Products:
The Kit requires the use of the Vortex Adapter (SKU #AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie® 2 and holds 12 tubes.

GeneJET Viral DNA/RNA Purification Kit (Thermo Scientific™)

Thermo Scientific GeneJET Viral DNA and RNA Purification Kit is designed for rapid and efficient purification of high quality viral nucleic acids from various human and mammalian liquid samples, and is compatible with common sample preservatives like EDTA, citrate, and heparin.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column. Viral nucleic acids from lysed samples bind to the column membrane while impurities are effectively removed during subsequent washing and centrifugation steps. Ready-to-use nucleic acids are eluted from the column.

The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA and RNA can be directly used in PCR, qPCR, or other nucleic acid based assays.

Highlights

• Efficient purification of viral DNA and RNA from human and mammalian plasma, serum, blood, and cell-free samples
• Linear yields from a wide range of viral titers
• Tested with a variety of DNA and RNA viruses (HIV, HBV, HCV, HPV, CMV, EBV)
• Downstream compatibility with (RT)-PCR, (RT)-qPCR, and other nucleic acid based assays.

Applications

• Downstream use in qualitative and quantitative nucleic acid based assays, e.g. (RT)-PCR, (RT)-qPCRIncludes Column Preparation Liquid Lysis Solution Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Eluent Proteinase K Carrier RNA Spin Columns preassembled with Wash Tubes Wash Tubes (2 mL)

MagMAX™ Microbiome Lysis Solution (Applied Biosystems™)

This lysis buffer is included in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42357, and A42358). It is made available separately for applications that require more lysis solution than is provided in the kit.

MagMAX™ Total RNA Elution Buffer (Applied Biosystems™)

This elution buffer is included in the MagMAX-96 Total RNA Isolation Kit (Cat. No. AM1830) and MagMAX-96 Viral RNA Isolation Kit (AM1836). It is made available here for separate purchase.

MagMAX™ DNA Multi-Sample Ultra 2.0 Wash I Solution (Applied Biosystems™)

This Wash I solution is included in the MagMAX DNA Multi-Sample Ultra 2.0 Kit. It is available separately for applications that require more Wash Isolution than is provided in the kit.

MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with PAXgene™ Blood RNA Tubes (Invitrogen™)

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.

• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
• Simple magnetic bead workflow—no spin columns
• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors

Maximize Total RNA Yields From Blood Samples
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from PAXgene® Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-18 µg from a single PAXgene® Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit performs exceptionally well at purifying miRNA with total RNA from PAXgene® Blood RNA Tubes without having to employ a separate miRNA protocol. One protocol does it all.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from PAXgene® Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.

Flexible Purification Formats
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods.
• For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step.
• For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process.
• For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).

Rapid Processing Time
Processing times will vary based on format selected, but twelve PAXgene® Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.

An Alternative to Full Purification
For users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from PAXgene® Blood RNA tubes without conventional RNA purification.

One-Stop Shopping
MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Yield (Range): 3 - 18µg
• Quality (RIN) ): ≥ 8
• Quality (A260⁄280) ): 1.8 – 2.2
• gDNA (w⁄w) ): <1.56%
• Equipment Needed :
     *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with
     *50 mL conical tube adapters
     *50ml conical tubes
     *For tube format users: Tabletop microcentrifuge or picocentrifuge
• Number of Preps: 96 preparations (blood tubes)

NOTE: Blood tubes and magnetic stands are sold separately.

NOTE: Blood tubes and magnetic stands are sold separately.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

PAXgene® and PreAnalytiX® are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein.

MagMAX™ CORE Nucleic Acid Purification Kit + MagMAX™ CORE Mastitis & Panbacteria Module (Applied Biosystems™)

The Applied Biosystems MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

Learn more about the MagMAX CORE Nucleic Acid Purification Kit >

MagMAX CORE Mastitis & Panbacteria Module
The MagMAX CORE Mastitis & Panbacteria Module is offered here in combination with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of all bacteria, especially those important in the detection of mastitis in cattle and from challenging matrices like mastitic milk.

We have included a specially optimized lysis solution, along with an adjusted extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram-positive bacteria.

Features of this combined product include:
Convenience—a complete solution using the same CORE chemistry
Easy—no centrifugation and no aspiration steps
Fast—less than 1 hour for 96 samples
Flexible—appropriate for fresh, frozen, or preserved milk samples, including samples with high somatic cell count

The MagMAX CORE kit and the MagMAX CORE Mastitis & Panbacteria Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.

PureLink™ Expi Endotoxin-Free Buffer Set (Invitrogen™)

The PureLink Expi Endotoxin-Free Buffer Set provides the same buffers included in the PureLink Expi Endotoxin-Free plasmid purification kits, made available separately here for those applications that would benefit from additional buffers. The Buffer Set includes all buffers and endotoxin-free components needed to enable 30 Maxi, 4 Mega, or 2 Giga PureLink Expi Endotoxin-Free plasmid purifications. Specifically, the buffer set includes RNAse A, resuspension buffer (R3), lysis buffer (L7), precipitation buffer (N3), equilibration buffer (EQ1), wash buffer (W8), elution buffer (E4), TE buffer (TE), endotoxin removal buffer, lysis indicator, and endotoxin-free water.

RNAqueous™-Midi Total RNA Isolation Kit (Invitrogen™)

The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.
• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

ChargeSwitch™ PCR Clean-Up Kit (Invitrogen™)

• Sample size 25-50 µl
• High recovery of DNA fragments
• Rapid, one tube, 5 minute protocol

ChargeSwitch® PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts (Figure 1). This kit is scalable for use on liquid-handling robots. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

GeneJET PCR Purification Kit (Thermo Scientific™)

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 µg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Highlights

Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
Fast: procedure takes only 5 minutes
Convenient: spin columns are capped and assembled with collection tubes

Applications

• Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:
FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
In vitro transcription
In situ hybridization
• Labeling
• Cloning

iPrep™ ChargeSwitch™ Buccal Cell Kit (Invitrogen™)

• Increased DNA yields and purity, allowing for more tests per sample and long-term archiving

• Multiple applications (e.g., databasing, human identification, clinical research) from the same sample

• Ethanol- and guanidine-free extraction for less inhibition of downstream applications

Buccal swabs represent a simple, noninvasive, and widely used method for collecting DNA. Isolating DNA from swabs is now easier than ever, thanks to the combined power of the the iPrep™ ChargeSwitch® Buccal Cell Kit and the iPrep™ Purification Instrument. This combination provides for fast lysis before automated processing-purified DNA can be obtained from cell samples in as little as 40 minutes, including a 20 minute lysis step. Additionally, youll experience less inhibition of downstream processes such as STR (Figure 1) and multiplex analysis, with higher yields and purity (Table 1).

Designed specifically for use with the iPrep™ Purification Instrument, the iPrep™ ChargeSwitch® Forensic Kit takes advantage of the powerful ChargeSwitch® technology, a major advance in DNA purification. This technology is based on a unique, ionizable nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification is achieved through a simple three-step procedure in aqueous buffers, and avoids the use of guanidine, ethanol, and other troublesome reagents.

Contents and Storage:
iPrep™ ChargeSwitch® Buccal Cell Kit provides all reagents (supplied in prefilled cartridges), tubes, and tips needed for isolating DNA from buccal cell samples. Reagent cartridges can be stored at room temperature; store lysis buffer and proteinase K means at 4°C.

MICROBEnrich™ Kit (Invitrogen™)

For the separation and isolation of bacterial RNA from mixed samples. The Ambion® kit includes sufficient reagents for depleting 500 µg of host cell RNA (20 reactions x 25 µg RNA) from a complex host-bacterial RNA mixture. Gene expression analysis following host-bacterial interactions is revolutionizing our understanding of the host response to bacterial infection. Numerous studies have utilized microarray analysis to follow host cell transcriptome alterations in response to interactions with bacteria. However, because no method has existed to isolate bacterial RNA away from host cell RNA, the corresponding analyses of bacterial transcriptomes has been impossible. The MICROBEnrich™ Kit enables whole genome microarray expression analyses of bacterial RNA after host-bacterial interactions.

Start With Any Total RNA Sample
For microarray analysis and other sensitive applications, a DNase treatment is recommended to remove contaminating genomic DNA from the total RNA prior to the MICROBEnrich™ Kit procedure.

Remove >90% of Mammalian RNA from Complex Total RNA Mixtures
The MICROBEnrichKituses hybridization capture technology (patent pending) to remove human, mouse, and rat RNA (both mRNA and rRNA) from complex host-bacterial RNA populations, leaving behind enriched microbial total RNA. In the first step of the procedure, host-bacterial total RNA is incubated with a mixture of capture oligonucleotides that bind the mammalian 18S and 28S rRNAs and polyadenylated RNAs. Next, the rRNA/oligo nucleotide hybrids and all polyadenylated mRNAs are removed from the mixture with oligonucleotide-derivatized magnetic beads. After magnetic separation, the bacterial RNA remaining in the supernatant and can be precipitated with ethanol. An Agilent 2100 bioanalyzer electropherogram (see Figure) shows nearly complete removal (>95%) of the host 18S and 28S rRNAs from a mixed sample. Bacterial mRNAs purified with the MICROBEnrich™ Kit are better templates than total RNA for downstream applications and therefore deliver dramatic increases in sensitivity when used in applications such as array analysis (see Figure).

The MICROBEnrich™ Kit Will Work With Any Bacterial Species
The MICROBEnrich Kit was optimized using E. coli RNA in a background of human, mouse, and rat RNA. However, the kit should perform well with RNA from any bacterial species. Although many mammalian host species may be compatible with the MICROBEnrichKit, only human, mouse, and rat rRNA sequences were used for the design and optimization of the capture oligonucleotides.

Accessory Products:
A magnetic stand is required for use this kit. They are available in several formats, including the Single Place Magnetic Stand (SKU #AM10026), 6 Tube Magnetic Stand (SKU #AM10055), and 96-well Magnetic Stands (SKU #AM10027 and #AM10050). The RiboPure™-Bacteria Kit (SKU #AM1925) is ideal for the purification of host-bacterial cell total RNA. The TURBO™ DNA-free™ or DNA-free™ DNase Treatment and Removal Reagents (SKU #AM1907 and AM1906, respectively) are recommended for microarray analysis and other applications where the removal of contaminating genomic DNA is needed.After the MICROBEnrich™ procedure, bacterial mRNA can be purified from the total RNA using the MICROBExpress™ Bacterial mRNA Enrichment Kit (SKU #AM1905). This technology enables >95% removal of prokaryotic 16S and 23S rRNAs.