Shop All RNA Purification Kits

RNAqueous™-96 Automated Kit Invitrogen™

The Ambion® RNAqueous®-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous®-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.

Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with Tempus™ Blood RNA Tubes Invitrogen™

The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 µl aliquot of human whole blood collected in Tempus™ Blood RNA tubes without conventional RNA purification. This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA. This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications.

• Detect total RNA (including miRNA) from Tempus™ Blood RNA Tubes without purification
• Fast—Prepare 96 samples for TaqMan®-based real-time RT-PCR in under 1 hour
• Robust—500 µl sample processed at room temperature with results equivalent to those from purified RNA
• Flexible Format—Samples can be prepared in microfuge tubes or manual⁄automated 96-well plates

Simple Workflow Without Sacrificing Sensitivity & Specificity
The process consists of pelleting and washing a 500uL aliquot from the blood tube, followed by a unique digestion⁄DNase step which preserves RNA integrity. The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps. Up to 96 samples can be prepared in less than an hour, half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort. The resulting lysate can go directly into one-step or two-step qRT-PCR reactions or stored for later use (1 hour at 4°C or 5 months frozen). This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers. The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents:
• Applied Biosystems’ High Capacity RNA-to-cDNA™ Kit and TaqMan® Gene Expression Master Mix
• Invitrogen’s SuperScript® VILO™ cDNA Synthesis Kit and TaqMan® Gene Expression Master Mix
• Applied Biosystems’ TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II
• Applied Biosystems’ TaqMan® One-Step RT-PCR Master Mix Reagents Kit
Reverse transcription reagents for cDNA synthesis, TaqMan® master mixes, and TaqMan® assays for real-time PCR analysis are sold separately and are not included with the kit.

The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits. Please see the user documentation for more details.

Screen, Discover, or Validate
For users with specific genes of interest, the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled Tempus™ Blood RNA Tube per sample). The remainder of the blood tube can be archived for later use or purified as needed.

Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease.

miRNA Analysis
With the growing focus on non-coding RNA and its regulatory role in development and disease, it’s reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. Our scientists have tested the lysate with the Applied Biosystems TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II against a large panel of TaqMan® microRNA assays and generated results equal to or better than those of the leading competitor’s miRNA purification product.

Reduced Footprint & Less Waste = More Savings
Competitive products, especially column-based methods, generate considerable amounts of plastic and biohazardous waste per sample (spin columns, tips, microfuge tubes, and reagents). The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR simplifies the workflow so the total plastic waste is 75% less by weight for a comparable number of samples processed via columns. The kit generates no organic waste. It also has a space-saving footprint to maximize freezer capacity. Reduced hazardous waste disposal and less plastic consumption translate into cost savings – as well as reducing the environmental impact.

For Users Needing Full Purification: If conventional RNA purification is still required for the remnant blood tube, one option is the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit. This product maximizes your yield of total RNA from Tempus™ Blood RNA Tubes using proven magnetic bead-based separation technology.

One-Stop Shopping: The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR is compatible with our suite of products (including RT and PCR master mixes that must be purchased separately). Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Compatibility—Tempus™ Blood RNA Tubes
• Processing Time—Under 1 hour
• Format— microfuge tubes, 96-well plates
• Equipment Needed—Standard centrifuge capable of 5,000 x g with either 96-well plate or microfuge tube adaptors and 96-well plate or microfuge tube vortexer
• Number of Preps —200 reactions

NOTE: Blood tubes, reverse transcription reagents for cDNA synthesis, and TaqMan® master mixes for real-time PCR analysis are sold separately.

For Research Use Only. Not for use in diagnostics procedures.

GeneJET Whole Blood RNA Purification Mini Kit Thermo Scientific™

Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.

Highlights

Efficient – up to 1.8 µg of total RNA from 500 µL of human blood
Convenient – spin columns are capped and assembled with collection tubes
Pure – RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
Fast and simple – procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysis
Compatible – suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluids

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assaysncludes Lysis Buffer Wash Buffer WB 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with collection tubes) Collection tubes, 2 mL Collection tubes, 1.5 mL Detailed Protocol

RiboPure™ RNA Purification Kit, yeast Invitrogen™

For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Ambion® kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.

• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents

It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).

Accessory Products:
The Kit requires the use of the Vortex Adapter (SKU #AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie® 2 and holds 12 tubes.

PureLink™ Total RNA Blood Kit Invitrogen™

PureLink® Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 µl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Simple RNA Purification from Whole Blood
PureLink® Total RNA Blood Kit is designed for purifying from 50 to 500 µl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink® Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink® Total RNA Blood Kit.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
Northern Blotting Kits & Reagents
Nuclease protection assays
Microarray
• cDNA synthesis
Real-Time PCR (qPCR)

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagMAX™-96 Total RNA Isolation Kit Invitrogen™

The MagMAX™-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions. Features of the MagMAX™-96 Total RNA Isolation Kit include:

• Enhanced lysis/binding conditions to accommodate larger sample inputs
• Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity
• Includes protocol for RNA isolation from plant tissues
• 96 reactions per kit suitable for moderate throughput users

Benefits of MagMAX™ magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Accommodates diverse sample types
The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 106 cells, as well as small plant and mammalian tissue samples (up to 10 mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit, and downloadable protocols for the use of this kit with specific liquid handling systems are available at the Automation Resource.

Accessory products
Lysis/Binding Solution Concentrate (AM8500), Wash Solution 1 Concentrate (AM8504), and Wash Solution 2 Concentrate (AM8640) are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid (AM9690) can be used in conjunction with the MagMAX™-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.

Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with PAXgene™ Blood RNA Tubes Invitrogen™

The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 µl aliquot of human whole blood collected in PAXgene® Blood RNA tubes without conventional RNA purification. This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA. This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications.

• Detect total RNA (including miRNA) from PAXgene® Blood RNA Tubes without purification
• Fast—Prepare 96 samples for TaqMan®-based real-time RT-PCR in under 1 hour
• Robust—500 µl sample processed at room temperature with results equivalent to those from purified RNA
• Flexible Format—Samples can be prepared in microfuge tubes or manual⁄automated 96-well plates.

Simple Workflow Without Sacrificing Sensitivity & Specificity
The process consists of pelleting and washing a 500uL aliquot from the blood tube, followed by a unique digestion⁄DNase step which preserves RNA integrity. The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps. Up to 96 samples can be prepared in less than an hour, half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort. The resulting lysate can go directly into one-step or two-step qRT-PCR reactions or stored for later use (1 hour at 4°C or 5 months frozen). This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers. The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents:

• Applied Biosystems’ High Capacity RNA-to-cDNA™ Kit and TaqMan® Gene Expression Master Mix
• Invitrogen’s SuperScript® VILO™ cDNA Synthesis Kit and TaqMan® Gene Expression Master Mix
• Applied Biosystems’ TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II
• Applied Biosystems’ TaqMan® One-Step RT-PCR Master Mix Reagents Kit
Reverse transcription reagents for cDNA synthesis, TaqMan® master mixes, and TaqMan® assays for real-time PCR analysis are sold separately and are not included with the kit.

The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits. Please see the user documentation for more details.

Screen, Discover, or Validate
For users with specific genes of interest, the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled PAXgene® Blood RNA Tube per sample). The remainder of the blood tube can be archived for later use or purified as needed.

Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease.

miRNA Analysis
With the growing focus on non-coding RNA and its regulatory role in development and disease, it’s reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. Our scientists have tested the lysate with the Applied Biosystems TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II against a large panel of TaqMan® microRNA assays and generated results equal to or better than those of the leading competitor’s miRNA purification product.

Reduced Footprint & Less Waste = More Savings
Competitive products, especially column-based methods, generate considerable amounts of plastic and biohazardous waste per sample (spin columns, tips, microfuge tubes, and reagents). The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR simplifies the workflow so the total plastic waste is 75% less by weight for a comparable number of samples processed via columns. The kit generates no organic waste. It also has a space-saving footprint to maximize freezer capacity. Reduced hazardous waste disposal and less plastic consumption translate into cost savings – as well as reducing the environmental impact.

For Users Needing Full Purification: If conventional RNA purification is still required for the remnant blood tube, one option is the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit. This product maximizes your yield of total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based separation technology.

One-Stop Shopping: The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR is compatible with our suite of products (including RT and PCR master mixes that must be purchased separately). Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Compatibility— PAXgene® Blood RNA Tubes
• Processing Time—Under 1 hour
• Format— microfuge tubes, 96-well plates
• Equipment Needed—Standard centrifuge capable of 5,000 x g with either 96-well plate or microfuge tube adaptors and 96-well plate or microfuge tube vortexer
• Number of Preps —200 reactions

NOTE: Blood tubes, reverse transcription reagents for cDNA synthesis, and TaqMan® master mixes for real-time PCR analysis are sold separately.

For Research Use Only. Not for use in diagnostics procedures.

PAXgene® and PreAnalytiX® are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein.

Mouse RiboPure™-Blood RNA Isolation Kit Invitrogen™

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion® kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNAlater®, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater® Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 µg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 µg from 0.5 mL blood.

RiboPure™ RNA Purification Kit, blood Invitrogen™

The RiboPure™-Blood Kit is for the isolation of high-quality RNA directly from whole blood. The kit combines two methods of RNA purification to efficiently remove blood components (protein, heme, genomic DNA, and RNases) that can compromise downstream procedures. The kit includes sufficient reagents for 40 RNA purifications from whole blood samples ranging from 0.3–0.5 mL each. Advantages of the RiboPure™-Blood Kit:

• Purify total RNA directly from whole blood—without prior recovery of WBCs
• Simple, fast procedure—from blood to RNA in less than 30 minutes
• Includes RNAlater® Solution—store blood samples 3 days or more at room temperature
• Includes DNA-free™ Reagents—to ready RNA for RT-PCR and array analysis

Applications
The RiboPure™-Blood Kit produces high yields of pure RNA directly from whole blood, eliminating the need to isolate WBCs prior to RNA purification. RNA recovered using the RiboPure™-Blood Kit can be used in the most challenging applications, including real-time RT-PCR and array analysis. Average yields of RNA are between 2–4 µg/0.5 mL of whole blood (variation is due to differences in WBC numbers between samples).

Stabilize RNA in whole blood
When collecting blood samples for RNA analysis, storage of the samples prior to RNA isolation is often required. The RiboPure™-Blood Kit contains RNAlater® Solution for the stabilization of RNA in whole blood. It also "freezes" the gene expression profile of the cells. Treated samples can be safely stored at ambient temperature for extended periods of time (up to three days or more). Blood samples stored in RNAlater® Solution yield RNA of comparable quality to blood samples processed directly (see figure).

Simple procedure yields highly pure RNA
The RiboPure™-Blood procedure consists of three steps: 1) addition of Lysis Solution directly to RNAlater®-treated or fresh whole blood, 2) initial RNA purification by phenol/chloroform extraction, and 3) final RNA purification on a glass fiber filter. An additional DNase treatment can then be performed with the DNA-free™ Reagents included in the kit. The entire procedure, including DNase treatment, can be completed in less than 1 hour.

Accessory products
The RNA can subsequently be enriched for white blood cell (WBC) mRNA using our GLOBINclear™ Kits to selectively remove globin transcripts. The Mouse RiboPure™-Blood RNA Isolation Kit (Cat. No. AM0951) is also available for the recovery of total RNA—and optionally microRNA—directly from mouse or rat blood. Mouse RiboPure™-Blood includes sample tubes preloaded with RNAlater® Solution to prevent RNA degradation and preserve RNA profiles.

mRNA Catcher™ PLUS Purification Kit Invitrogen™

The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

Robust performance—high binding capacity per well (>80 ng/well)
Reproducible results—low CVs from well-to-well and plate-to-plate (~2%)
Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 µL blood)
Compatible plate design—may be used with most PCR cyclers

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

GLOBINclear™ Kit, human, for globin mRNA depletion Invitrogen™

The GLOBINclear™-Human Kit uses a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood. Advantages of the GLOBINclear™-Human Kit:

• Removes >95% of unwanted globin mRNA from whole blood total RNA
• Detects up to 50% more genes—analyze previously undetected genes
• Achieves greater representation of the genome, low 3'/5' ratios
• Avoids RNase H treatments that can degrade mRNA and alter expression profiles

Using whole blood total RNA samples
It is widely known that expression array data generated from whole blood total RNA samples have reduced detection sensitivity compared to data from fractionated blood samples. Examination of whole blood total RNA reveals a dominant band in the 600–700 base region. This band represents globin mRNA, which is expressed at high levels in red blood cells (RBCs) and reticulocytes (RBC precursors). Up to 70% of the mRNA (by mass) in whole blood total RNA are globin transcripts. Array analysis using total RNA containing a high percentage of globin mRNA has been shown to decrease present calls, decrease call concordance, and increase signal variation.

Using the GLOBINclear Kit
The GLOBINclear™ kit uses a novel hybridization technology that takes advantage of the strength of biotin/streptavidin binding, the specificity of nucleic acid hybridization, and the convenience of magnetic separations. There are no harsh RNase H enzymatic treatments that may compromise the expression profile by degrading mRNAs other than globin mRNA. The resulting mRNA is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR. The enrichment delivers a significant increase in Percent Present calls, decreased sample-to-sample variability, and low 3'/5' ratios.

Accessory product
The kit requires the use of a magnetic stand. The DynaMag-2 Magnetic Stand (Cat. No. 12321D) is recommended.

PureLink™ FFPE RNA Isolation Kit Invitrogen™

The PureLink® FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens for retrospective basic and clinical biological studies of gene expression without the use of toxic organic solvents for deparaffinization.
• Offers a rapid and environmentally friendly procedure for the extraction of RNA from FFPE tissue specimens
• Isolates RNA with maximum yield and minimum degradation

Rapid and Non-Toxic Isolation Procedure
The PureLink® FFPE RNA Isolation Kit employs a simple heating procedure for deparaffinization, instead of relying on the use of toxic organic solvents such as xylene. RNA isolation can be completed in 30 - 40 minutes instead of days, as is required for older FFPE isolation methods (Figure 1). The heating procedure for deparaffinization partly restores RNA template structure for cDNA synthesis and improves template quality for reverse transcription.

High Yield, High Quality RNA
The PureLink® FFPE RNA Isolation Kit offers higher yields and purity as compared to other commercially available RNA purification systems, allowing for RT-PCR products over 1 kb in length (Figure 2). In addition, the PureLink® FFPE RNA Isolation Kit minimizes genomic DNA contamination of the purified RNA sample and provides an option for DNase digestion.

Purified Total RNA is Suitable for Use with a Variety of Downstream Applications and Products
RNA extracted from FFPE specimens is suitable for downstream applications such as reverse transcription, real-time PCR (qPCR), real-time RT-PCR (qRT-PCR), and microarray analysis.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Poly(A)Purist™ MAG Kit with Manual Invitrogen™

Ambion® Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures. Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA.

• Highest recovery of mRNA of any available kit
• High stringency for lowering residual rRNA content
• Single round of selection sufficient for most applications

The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure). The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography. The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability. The entire selection procedure can be completed in as little as 45 minutes (see Figure).

Accessory Products:
A magnetic stand is required for use this kit. They are available in several formats, including the Single Place Magnetic Stand (SKU #AM10026) and the 6 Tube Magnetic Stand (SKU #AM10055).

MagMAX™ Plant RNA Isolation Kit Applied Biosystems™

The MagMAX Plant RNA Isolation Kit is designed for automated high-throughput or manual purification of total RNA from wide variety of plant species and tissue types. It utilizes paramagnetic bead technology, enabling high yields and robust performance. The high binding capacity, uniform particle size, and rapid magnetic response of MagMAX magnetic beads makes the technology ideal for high-throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low- throughput users. The resulting high quality purified RNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, and other enzymatic reactions.

Versatile—suitable for any plant type and part, including woody, polyphenol-rich, and lignified samples
High yields of pure total RNA
High quality—isolates intact total RNA, free of inhibitors and gDNA contamination. Isolated RNA is suitable for long term storage at -20°C.
Flexible—easily adjustable for high-throughput automation platforms and manual processing

Applications
The MagMAX Plant RNA Isolation Kit is designed for fast extraction of high purity RNA suitable for all conventional molecular biology applications, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher Duo Prime or KingFisher Flex processors.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps.

Optimized bead surface chemistry
The Dynabeads MyOne Carboxylic Acid utilized in the MagMAX Plant RNA Isolation Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads magnetic technology ideal for the recovery of the RNA targets found in a variety of plant samples. Once captured, the RNA is then eluted in 100 µL and ready for downstream applications.

RNAqueous™-Midi Total RNA Isolation Kit Invitrogen™

The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.
• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
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