Shop All RNA Purification Kits

Mouse RiboPure™-Blood RNA Isolation Kit (Invitrogen™)

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion® kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNAlater®, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater® Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 µg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 µg from 0.5 mL blood.

Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with either PAXgene™ or Tempus™ Blood RNA Tubes (Invitrogen™)

The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 µl aliquot of human whole blood collected in PAXgene® or Tempus™ Blood RNA tubes without conventional RNA purification. This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA. This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications.

• Detect total RNA from PAXgene® (including miRNA) or Tempus™ Blood RNA Tubes without purification
• Fast—Prepare 96 samples for TaqMan®-based real-time RT-PCR in under 1 hour
• Robust—500 µl sample processed at room temperature with results equivalent to those from purified RNA
• Flexible Format—Samples can be prepared in microfuge tubes or manual⁄automated 96-well plates

Simple Workflow Without Sacrificing Sensitivity & Specificity
The process consists of pelleting and washing a 500uL aliquot from the blood tube, followed by a unique digestion⁄DNase step which preserves RNA integrity. The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps. Up to 96 samples can be prepared in less than an hour, half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort. The resulting lysate can go directly into one-step or two-step qRT-PCR reactions or stored for later use (1 hour at 4°C or 5 months frozen). This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers. The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents:
• Applied Biosystems’ High Capacity RNA-to-cDNA™ Kit and TaqMan® Gene Expression Master Mix
• Invitrogen’s SuperScript® VILO™ cDNA Synthesis Kit and TaqMan® Gene Expression Master Mix
• Applied Biosystems’ TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II
• Applied Biosystems’ TaqMan® One-Step RT-PCR Master Mix Reagents Kit
Reverse transcription reagents for cDNA synthesis, TaqMan® master mixes, and TaqMan® assays for real-time PCR analysis are sold separately.

The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits. Please see the user documentation for more details.

Screen, Discover, or Validate
For users with specific genes of interest, the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled PAXgene® or Tempus™ Blood RNA Tube per sample). The remainder of the blood tube can be archived for later use or purified as needed.

Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease.

miRNA Analysis
With the growing focus on non-coding RNA and its regulatory role in development and disease, it’s reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. Our scientists have tested the lysate with the Applied Biosystems TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II against a large panel of TaqMan® microRNA assays and generated results equal to or better than those of the leading competitor’s miRNA purification product.

Reduced Footprint & Less Waste = More Savings
Competitive products, especially column-based methods, generate considerable amounts of plastic and biohazardous waste per sample (spin columns, tips, microfuge tubes, and reagents). The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR simplifies the workflow so the total plastic waste is 75% less by weight for a comparable number of samples processed via columns. The kit generates no organic waste. It also has a space-saving footprint to maximize freezer capacity. Reduced hazardous waste disposal and less plastic consumption translate into cost savings – as well as reducing the environmental impact.

For Users Needing Full Purification:If conventional RNA purification is still required for the remnant blood tube, one option is the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit. This product maximizes your yield of total RNA from PAXgene® (including miRNA) or Tempus™ Blood RNA Tubes using proven magnetic bead-based separation technology.

One-Stop Shopping: The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR is compatible with our suite of products (including RT and PCR master mixes that must be purchased separately). Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Compatibility—PAXgene® or Tempus™ Blood RNA Tubes
• Processing Time—Under 1 hour
• Format— microfuge tubes, 96-well plates
• Equipment Needed—Standard centrifuge capable of 5,000 x g with either 96-well plate or microfuge tube adaptors and 96-well plate or microfuge tube vortexer
• Number of Preps —50 reactions

NOTE: Blood tubes, reverse transcription reagents for cDNA synthesis, and TaqMan® master mixes for real-time PCR analysis are sold separately.

For Research Use Only. Not for use in diagnostics procedures.

PAXgene® and PreAnalytiX® are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein.

Mouse RiboPure™-Blood RNA Isolation Kit with Manual (Invitrogen™)

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion® kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNAlater®, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater® Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 µg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 µg from 0.5 mL blood.

Wash Buffer B for Dynabeads™ mRNA Purification Kits (Invitrogen™)

This Wash Buffer B is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61006, 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer B than is provided in the kit.

Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with Tempus™ Blood RNA Tubes (Invitrogen™)

The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 µl aliquot of human whole blood collected in Tempus™ Blood RNA tubes without conventional RNA purification. This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA. This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications.

• Detect total RNA (including miRNA) from Tempus™ Blood RNA Tubes without purification
• Fast—Prepare 96 samples for TaqMan®-based real-time RT-PCR in under 1 hour
• Robust—500 µl sample processed at room temperature with results equivalent to those from purified RNA
• Flexible Format—Samples can be prepared in microfuge tubes or manual⁄automated 96-well plates

Simple Workflow Without Sacrificing Sensitivity & Specificity
The process consists of pelleting and washing a 500uL aliquot from the blood tube, followed by a unique digestion⁄DNase step which preserves RNA integrity. The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps. Up to 96 samples can be prepared in less than an hour, half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort. The resulting lysate can go directly into one-step or two-step qRT-PCR reactions or stored for later use (1 hour at 4°C or 5 months frozen). This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers. The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents:
• Applied Biosystems’ High Capacity RNA-to-cDNA™ Kit and TaqMan® Gene Expression Master Mix
• Invitrogen’s SuperScript® VILO™ cDNA Synthesis Kit and TaqMan® Gene Expression Master Mix
• Applied Biosystems’ TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II
• Applied Biosystems’ TaqMan® One-Step RT-PCR Master Mix Reagents Kit
Reverse transcription reagents for cDNA synthesis, TaqMan® master mixes, and TaqMan® assays for real-time PCR analysis are sold separately and are not included with the kit.

The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits. Please see the user documentation for more details.

Screen, Discover, or Validate
For users with specific genes of interest, the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled Tempus™ Blood RNA Tube per sample). The remainder of the blood tube can be archived for later use or purified as needed.

Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease.

miRNA Analysis
With the growing focus on non-coding RNA and its regulatory role in development and disease, it’s reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. Our scientists have tested the lysate with the Applied Biosystems TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II against a large panel of TaqMan® microRNA assays and generated results equal to or better than those of the leading competitor’s miRNA purification product.

Reduced Footprint & Less Waste = More Savings
Competitive products, especially column-based methods, generate considerable amounts of plastic and biohazardous waste per sample (spin columns, tips, microfuge tubes, and reagents). The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR simplifies the workflow so the total plastic waste is 75% less by weight for a comparable number of samples processed via columns. The kit generates no organic waste. It also has a space-saving footprint to maximize freezer capacity. Reduced hazardous waste disposal and less plastic consumption translate into cost savings – as well as reducing the environmental impact.

For Users Needing Full Purification: If conventional RNA purification is still required for the remnant blood tube, one option is the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit. This product maximizes your yield of total RNA from Tempus™ Blood RNA Tubes using proven magnetic bead-based separation technology.

One-Stop Shopping: The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR is compatible with our suite of products (including RT and PCR master mixes that must be purchased separately). Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Compatibility—Tempus™ Blood RNA Tubes
• Processing Time—Under 1 hour
• Format— microfuge tubes, 96-well plates
• Equipment Needed—Standard centrifuge capable of 5,000 x g with either 96-well plate or microfuge tube adaptors and 96-well plate or microfuge tube vortexer
• Number of Preps —200 reactions

NOTE: Blood tubes, reverse transcription reagents for cDNA synthesis, and TaqMan® master mixes for real-time PCR analysis are sold separately.

For Research Use Only. Not for use in diagnostics procedures.

MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes (Invitrogen™)

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from Tempus™ Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.

• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
• Simple magnetic bead workflow—no spin columns
• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors

Maximize Total RNA Yields From Blood Samples
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from Tempus™ Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-25 µg from a single Tempus™ Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from Tempus™ Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.

Flexible Purification Formats
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods.
• For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step.
• For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process.
• For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).

Rapid Processing Time
Processing times will vary based on format selected, but twelve Tempus™ Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.

An Alternative to Full Purification
For users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from Tempus™ Blood RNA tubes without conventional RNA purification.

One-Stop Shopping
MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Yield (Range): 3 - 25µg
• Quality (RIN) ): ≥ 7
• Quality (A260⁄280) ): 1.8 – 2.2
• gDNA (w⁄w) ): <1.56%
• Equipment Needed :
     *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with
     *50 mL conical tube adapters
     *50ml conical tubes
     *For tube format users: Tabletop microcentrifuge or picocentrifuge
• Number of Preps: 96 preparations (blood tubes)

NOTE: Blood tubes and magnetic stands are sold separately.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

MagMAX™-96 Blood RNA Isolation Kit (Invitrogen™)

This Ambion® kit is for the rapid, high-throughput purification of pathogen RNA from mammalian whole blood, milk, and other biofluids in 96-well plates. RNA purified using the kit is ideal for RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions.

• Easily isolate viral or total RNA from whole blood
• Save precious time—complete the protocol in less than 1 hour
• Walk away—integrate with established robotic platforms
• Validated with human, bovine, porcine, and ovine whole blood

Benefits of MagMAX™ Magnetic Bead-Based Purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high- efficiency binding, the bound RNA can be eluted in just 30–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Isolate RNA from as Little as 50 µl of Blood:
For the highest-quality RNA, use whole blood samples that have not been frozen. Approximately 300–500 ng of intact RNA can be consistently purified from 50 µl of human whole blood. Bovine, porcine, and ovine whole blood samples can also be used. Yields vary from species to species (see data). The recovered RNA is devoid of PCR inhibitors such as proteins and heme, commonly found in RNA isolated from blood.

Isolate Viral and Total RNA from Whole Blood Samples:
The MagMAX™-96 Blood RNA Isolation Kit eliminates sample preprocessing, which can cause sample mix-up and cross-contamination. The procedure is fast (<1 hour), simple, and well suited for automation. The kit can also be used to process fewer than 96 samples at one time and is compatible with either manual processing using multi-channel pipettors or processing with robotic liquid handlers. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource. The MagMAX™-96 Blood RNA Isolation Kit can also be used for total nucleic acid isolation by omitting the DNase treatment.

Accessory Products:
Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the kit that may also be purchased separately. A 96-well magnetic ring stand is required to use this kit. The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96-well U-bottom microplates. The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors. For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended.

MagMAX™ Plant RNA Isolation Kit (Applied Biosystems™)

The MagMAX Plant RNA Isolation Kit is designed for automated high-throughput or manual purification of total RNA from wide variety of plant species and tissue types. It utilizes paramagnetic bead technology, enabling high yields and robust performance. The high binding capacity, uniform particle size, and rapid magnetic response of MagMAX magnetic beads makes the technology ideal for high-throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low- throughput users. The resulting high quality purified RNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, and other enzymatic reactions.

Versatile—suitable for any plant type and part, including woody, polyphenol-rich, and lignified samples
High yields of pure total RNA
High quality—isolates intact total RNA, free of inhibitors and gDNA contamination. Isolated RNA is suitable for long term storage at -20°C.
Flexible—easily adjustable for high-throughput automation platforms and manual processing

Applications
The MagMAX Plant RNA Isolation Kit is designed for fast extraction of high purity RNA suitable for all conventional molecular biology applications, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher Duo Prime or KingFisher Flex processors.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps.

Optimized bead surface chemistry
The Dynabeads MyOne Carboxylic Acid utilized in the MagMAX Plant RNA Isolation Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads magnetic technology ideal for the recovery of the RNA targets found in a variety of plant samples. Once captured, the RNA is then eluted in 100 µL and ready for downstream applications.

MagMAX™-96 Total RNA Isolation Kit (Invitrogen™)

The MagMAX™-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions. Features of the MagMAX™-96 Total RNA Isolation Kit include:

• Enhanced lysis/binding conditions to accommodate larger sample inputs
• Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity
• Includes protocol for RNA isolation from plant tissues
• 96 reactions per kit suitable for moderate throughput users

Benefits of MagMAX™ magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Accommodates diverse sample types
The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 106 cells, as well as small plant and mammalian tissue samples (up to 10 mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit, and downloadable protocols for the use of this kit with specific liquid handling systems are available at the Automation Resource.

Accessory products
Lysis/Binding Solution Concentrate (AM8500), Wash Solution 1 Concentrate (AM8504), and Wash Solution 2 Concentrate (AM8640) are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid (AM9690) can be used in conjunction with the MagMAX™-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.

RNAqueous™-Midi Total RNA Isolation Kit (Invitrogen™)

The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.
• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

PureLink™ Pro 96 total RNA Purification Kit (Invitrogen™)

The PureLink® Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

RiboMinus™ Transcriptome Isolation Kit, human/mouse (Invitrogen™)

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods.

The RiboMinus™ Transcriptome Isolation Kits enable:

Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

MagMAX™ mirVana™ Total RNA Isolation Kit (Applied Biosystems™)

Scalable isolation of RNA (including small RNAs)
The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.

The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX mirVana Total RNA Isolation Kit include:
• Automation-ready, phenol-free extraction
• Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
• Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays

One kit to isolate true total RNA from a diverse set of sample types
Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering “specialized” kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples.

The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:

• Serum/plasma
• Whole blood
• Tissue
• Cell culture
• Urine

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR, compatible with PAXgene™ Blood RNA Tubes (Invitrogen™)

The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR makes it possible to perform expression analysis directly from a 500 µl aliquot of human whole blood collected in PAXgene® Blood RNA tubes without conventional RNA purification. This simple procedure creates a lysate that can go directly into reverse transcription and real-time PCR with the sensitivity and specificity equivalent to conventionally purified RNA. This kit saves time, generates much less waste, and offers a simple workflow that is suitable for a few samples or can easily be incorporated into automated, high throughput applications.

• Detect total RNA (including miRNA) from PAXgene® Blood RNA Tubes without purification
• Fast—Prepare 96 samples for TaqMan®-based real-time RT-PCR in under 1 hour
• Robust—500 µl sample processed at room temperature with results equivalent to those from purified RNA
• Flexible Format—Samples can be prepared in microfuge tubes or manual⁄automated 96-well plates.

Simple Workflow Without Sacrificing Sensitivity & Specificity
The process consists of pelleting and washing a 500uL aliquot from the blood tube, followed by a unique digestion⁄DNase step which preserves RNA integrity. The preparation takes place entirely at room temperature unlike other products that require temperature-specific incubation steps. Up to 96 samples can be prepared in less than an hour, half the time it takes for competitive spin column purification to process only 12 samples, with less hands-on effort. The resulting lysate can go directly into one-step or two-step qRT-PCR reactions or stored for later use (1 hour at 4°C or 5 months frozen). This straightforward procedure takes place in the same sample processing well, from start to finish, meaning no sample transfers. The real-time RT-PCR data generated from the lysate is equivalent to results obtained from conventionally purified RNA from the same tubes and has been validated with the following qPCR reagents:

• Applied Biosystems’ High Capacity RNA-to-cDNA™ Kit and TaqMan® Gene Expression Master Mix
• Invitrogen’s SuperScript® VILO™ cDNA Synthesis Kit and TaqMan® Gene Expression Master Mix
• Applied Biosystems’ TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II
• Applied Biosystems’ TaqMan® One-Step RT-PCR Master Mix Reagents Kit
Reverse transcription reagents for cDNA synthesis, TaqMan® master mixes, and TaqMan® assays for real-time PCR analysis are sold separately and are not included with the kit.

The total number of reverse transcription and PCR reactions possible from one starting sample depends on the minimum sample requirements of the desired RT-PCR kits. Please see the user documentation for more details.

Screen, Discover, or Validate
For users with specific genes of interest, the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR was designed to enable quick screening from a small amount of starting sample (approximately 5% of the total volume of a properly filled PAXgene® Blood RNA Tube per sample). The remainder of the blood tube can be archived for later use or purified as needed.

Those who perform discovery or validation experiments with real-time RT-PCR analysis will save effort, time, and overall cost with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR – while employing a scalable process allowing labs bottlenecked by conventional purification to finally start processing samples with ease.

miRNA Analysis
With the growing focus on non-coding RNA and its regulatory role in development and disease, it’s reassuring to know these small but important RNA molecules can be detected properly with the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. Our scientists have tested the lysate with the Applied Biosystems TaqMan® MicroRNA Reverse Transcription Kit and TaqMan® Universal Master Mix II against a large panel of TaqMan® microRNA assays and generated results equal to or better than those of the leading competitor’s miRNA purification product.

Reduced Footprint & Less Waste = More Savings
Competitive products, especially column-based methods, generate considerable amounts of plastic and biohazardous waste per sample (spin columns, tips, microfuge tubes, and reagents). The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR simplifies the workflow so the total plastic waste is 75% less by weight for a comparable number of samples processed via columns. The kit generates no organic waste. It also has a space-saving footprint to maximize freezer capacity. Reduced hazardous waste disposal and less plastic consumption translate into cost savings – as well as reducing the environmental impact.

For Users Needing Full Purification: If conventional RNA purification is still required for the remnant blood tube, one option is the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit. This product maximizes your yield of total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based separation technology.

One-Stop Shopping: The Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR is compatible with our suite of products (including RT and PCR master mixes that must be purchased separately). Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Compatibility— PAXgene® Blood RNA Tubes
• Processing Time—Under 1 hour
• Format— microfuge tubes, 96-well plates
• Equipment Needed—Standard centrifuge capable of 5,000 x g with either 96-well plate or microfuge tube adaptors and 96-well plate or microfuge tube vortexer
• Number of Preps —200 reactions

NOTE: Blood tubes, reverse transcription reagents for cDNA synthesis, and TaqMan® master mixes for real-time PCR analysis are sold separately.

For Research Use Only. Not for use in diagnostics procedures.

PAXgene® and PreAnalytiX® are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein.

MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with PAXgene™ Blood RNA Tubes (Invitrogen™)

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.

• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
• Simple magnetic bead workflow—no spin columns
• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors

Maximize Total RNA Yields From Blood Samples
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from PAXgene® Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-18 µg from a single PAXgene® Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit performs exceptionally well at purifying miRNA with total RNA from PAXgene® Blood RNA Tubes without having to employ a separate miRNA protocol. One protocol does it all.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from PAXgene® Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.

Flexible Purification Formats
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods.
• For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step.
• For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process.
• For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).

Rapid Processing Time
Processing times will vary based on format selected, but twelve PAXgene® Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.

An Alternative to Full Purification
For users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from PAXgene® Blood RNA tubes without conventional RNA purification.

One-Stop Shopping
MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Yield (Range): 3 - 18µg
• Quality (RIN) ): ≥ 8
• Quality (A260⁄280) ): 1.8 – 2.2
• gDNA (w⁄w) ): <1.56%
• Equipment Needed :
     *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with
     *50 mL conical tube adapters
     *50ml conical tubes
     *For tube format users: Tabletop microcentrifuge or picocentrifuge
• Number of Preps: 96 preparations (blood tubes)

NOTE: Blood tubes and magnetic stands are sold separately.

NOTE: Blood tubes and magnetic stands are sold separately.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

PAXgene® and PreAnalytiX® are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein.