Shop All RNA Purification Kits

GeneJET RNA Purification Kit (Thermo Scientific™)

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
Efficient – high yields of total RNA.
Fast – 15 minutes after lysis step
Pure – an A260/280 ratio > 1.9
Convenient – spin columns are capped and assembled with collection tubes

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assayIncludes Proteinase K Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Collection Tubes) Collection Tubes (2 ml) Collection Tubes (1.5 ml) Detailed Protocol

Dynabeads™ mRNA Purification Kit (for mRNA purification from total RNA preps) (Invitrogen™)

The Dynabeads® mRNA Purification Kit rapidly isolates the mRNA transcriptome in typically 15 minutes, delivering pure, intact mRNA. The kit is designed to specifically target, capture, and purify mRNA molecules from total RNA preparations. Advantages of using the Dynabeads® mRNA Purification Kit:

• Obtain pure mRNA in typically 15 minutes
• Recover and enrich the transcriptome efficiently
• Prepare mRNA that is suitable for nearly every downstream application

Pure, rapid mRNA isolation
The Dynabeads® mRNA Purification Kit contains magnetic beads for the isolation of the mRNA transcriptome from any total eukaryotic RNA preparation (see figure). Dynabeads® magnetic beads are uniform in size and highly mobile in solution (see figure). This enables the bead capture surface to quickly and continuously interact with the entire total RNA sample during the mRNA capture phase. The ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured resulting in cleaner, more sensitive results (see figure). Within about 15 minutes, pure mRNA is isolated and ready for use in downstream applications.

A straightforward enrichment procedure
The Dynabeads® mRNA Purification Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. Superparamagnetic Dynabeads®, coupled to oligo-(dT)25, are first equilibrated with Binding Buffer, and then mixed with purified total RNA. The beads are then washed to remove contaminating RNA species, and then mRNA is eluted in as little as 5 µL of 10 mM Tris-HCl. The entire process is facilitated by the use of a neodymium magnet (purchased separately), which allows for the quick and efficient immobilization of the magnetic beads during buffer changes. RNA is suitable for all downstream molecular applications, including:

• Gene cloning
• cDNA synthesis, cDNA library construction
• RT-PCR, quantitative RT-PCR
• RPA (Ribonuclease Protection Assay)
• Subtractive hybridization
• Dot/slot hybridization
• Primer extension

RNAqueous™-96 Automated Kit (Invitrogen™)

The Ambion® RNAqueous®-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous®-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.

MagMAX™ Plant RNA Isolation Kit (Applied Biosystems™)

The MagMAX Plant RNA Isolation Kit is designed for automated high-throughput or manual purification of total RNA from wide variety of plant species and tissue types. It utilizes paramagnetic bead technology, enabling high yields and robust performance. The high binding capacity, uniform particle size, and rapid magnetic response of MagMAX magnetic beads makes the technology ideal for high-throughput automatic nucleic acid purification, as well as for manual nucleic acid purification by low- throughput users. The resulting high quality purified RNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, and other enzymatic reactions.

Versatile—suitable for any plant type and part, including woody, polyphenol-rich, and lignified samples
High yields of pure total RNA
High quality—isolates intact total RNA, free of inhibitors and gDNA contamination. Isolated RNA is suitable for long term storage at -20°C.
Flexible—easily adjustable for high-throughput automation platforms and manual processing

Applications
The MagMAX Plant RNA Isolation Kit is designed for fast extraction of high purity RNA suitable for all conventional molecular biology applications, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher Duo Prime or KingFisher Flex processors.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps.

Optimized bead surface chemistry
The Dynabeads MyOne Carboxylic Acid utilized in the MagMAX Plant RNA Isolation Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads magnetic technology ideal for the recovery of the RNA targets found in a variety of plant samples. Once captured, the RNA is then eluted in 100 µL and ready for downstream applications.

RiboMinus™ Transcriptome Isolation Kit, yeast (Invitrogen™)

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

GeneJET Whole Blood RNA Purification Mini Kit (Thermo Scientific™)

Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.

Highlights

Efficient – up to 1.8 µg of total RNA from 500 µL of human blood
Convenient – spin columns are capped and assembled with collection tubes
Pure – RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
Fast and simple – procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysis
Compatible – suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluids

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assaysncludes Lysis Buffer Wash Buffer WB 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with collection tubes) Collection tubes, 2 mL Collection tubes, 1.5 mL Detailed Protocol

MagMAX™ mirVana™ Total RNA Isolation Kit (Applied Biosystems™)

Scalable isolation of RNA (including small RNAs)
The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.

The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX mirVana Total RNA Isolation Kit include:
• Automation-ready, phenol-free extraction
• Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
• Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays

One kit to isolate true total RNA from a diverse set of sample types
Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering “specialized” kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples.

The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:

• Serum/plasma
• Whole blood
• Tissue
• Cell culture
• Urine

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Wash Buffer A for Dynabeads™ mRNA Purification Kits (Invitrogen™)

This Wash Buffer A is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer A than is provided in the kit.

ChargeSwitch™ Total RNA Cell Kit (Invitrogen™)

The ChargeSwitch® Total RNA Cell Kit is ideal for the purification of up to 25 µg of total RNA from up to 1 × 106 cultured mammalian cells or from 1 ml overnight bacterial culture, including full-length RNA and small molecular weight RNA species. This kit offers a unique aqueous-based method for purifying total RNA from cultured mammalian or bacterial cells resulting in exceptionally clean and pure RNA with high integrity (Figure 1). ChargeSwitch® technology eliminates the use of common enzymatic inhibitors such as phenol, chaotropic salts or ethanol, resulting in exceptionally clean RNA. Due to its unique format, this kit also performs exceptionally well at concentrations useful for greater sensitivity in downstream analyses (Figure 3), yielding faster scientific results.

Features of The ChargeSwitch® Total RNA Cell Kit:

• Effectively captures up to 25 µg of total RNA from up to 1 × 106 cultured mammalian cells or 1 ml bacterial culture
• Purifies a wide range of transcripts including increasingly important small RNAs (smaller than 200 nucleotides)
• Provides the ability to purify greater yields of RNA from very small sample sizes
• Eliminates the use of common enzymatic inhibitors providing exceptionally clean RNA
• Results in improved sensitivity in downstream analyses
• Allows flexibility for high-throughput applications

The ChargeSwitch® technology format is scalable and is a highly-sensitive RNA extraction method for successful downstream applications, including gene expression studies, promoter studies, siRNA, gene regulation, transcriptional analysis, RT-PCR, and qRT-PCR. This unique format also allows flexibility for application in high-throughput automation.

How ChargeSwitch® Technology Works:
Based on a patented switchable chemistry, ChargeSwitch® purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge attracting RNA. At higher pH, the surface chemistry is neutral, thereby releasing any attraction to bound RNA (Figure 2).

RiboMinus™ Transcriptome Isolation Kit, bacteria (Invitrogen™)

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

iPrep™ Trizol™ Plus RNA Kit (Invitrogen™)

Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.

GeneJET RNA Cleanup and Concentration Micro Kit (Thermo Scientific™)

Thermo Scientific GeneJET RNA Cleanup and Concentration Micro Kit is designed for rapid and efficient concentration of prepurified RNA samples, as well as for RNA cleanup after DNase I treatment and other enzymatic reactions.

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Highlights

• Advanced column design—easy to open, load and handle.
• Elute in 6 to 10 µL volume.
• 85 to 100% recovery of a high integrity RNA.
• Fast and simple—procedure takes less than 5 minutes.

Applications:
• The purified RNA can be used in common downstream applications
• RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis

Dynabeads™ mRNA DIRECT™ Purification Kit (Invitrogen™)

The Dynabeads® mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications. Advantages of the Dynabeads® mRNA DIRECT™ Kit:

Fast—15 minute procedure yields pure, intact mRNA
Highly pure mRNA isolation—best choice upstream of cDNA synthesis
Sensitive mRNA isolation—enables cDNA synthesis and cDNA library construction from ultra-small starting samples

System overview
The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads® . Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol). 1 mg of Dynabeads® Oligo (dT)25 beads (200 µL) binds up to 2 µg of mRNA. A typical mammalian cell contains about 10–30 pg of total RNA, from which 1–5% is mRNA.

Poly(A)Purist™ MAG Kit (Invitrogen™)

Ambion® Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures. Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA.

• Highest recovery of mRNA of any available kit
• High stringency for lowering residual rRNA content
• Single round of selection sufficient for most applications

The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure). The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography. The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability. The entire selection procedure can be completed in as little as 45 minutes (see Figure).

Accessory Products:
A magnetic stand is required for use this kit. They are available in several formats, including the Single Place Magnetic Stand (SKU #AM10026) and the 6 Tube Magnetic Stand (SKU #AM10055).

LeukoLOCK™ Fractionation & Stabilization Kit with Manual

The Ambion® LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending), an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population. The kit includes sufficient reagents for 20 reactions. The LeukoLOCK™ System (SKU #AM1923) is optimized for use with human blood. Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications. The LeukoLOCK™ System employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and Ambion's RNAlater® Solution to stabilize the cells on the filter. By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications (see Figure).

LeukoLOCK™ Fractionation & Stabilization Kit
A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK™ filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma. After rinsing with phosphate-buffered saline, the filter is flushed with RNAlater® Solutionto stabilize the RNA in the captured leukocytes (see protocol schematic). The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature or for longer periods at –20°C or –80°C (see Figure).

Accessory Products:
LeukoLOCK™ Fractionation & Stabilization Kit is designed for use with the LeukoLOCK™ Total RNA Isolation Kit (SKU #AM1934). These kits are offered separately to accommodate blood sample collection at one location and sample processing at a different location, but may be purchased together as the LeukoLOCK™ Total RNA Isolation System (SKU #AM1923).