Shop All RNA Purification Kits

Tempus™ Spin RNA Isolation Kit Invitrogen™

This Applied Biosystems® product is for the fast, easy, glass fiber filter–based isolation of RNA from blood using standard laboratory equipment in about one hour. Contains sufficient reagents for RNA purification from 50 samples.

• Isolate high-quality RNA from blood, suitable for real-time PCR and microarray analysis
• Obtain highly stable RNA virtually free of RNase activity, contaminating gDNA, or RT-PCR inhibitors
• Easy workflow requires only standard laboratory centrifuges and ~1 hour of time
• No separate RBC lysis or proteinase K treatment required

Gene expression profiling and analysis of whole blood is becoming an increasingly important life science tool. Accurately measuring the molecular profile of blood-based RNA transcripts is a critical need in areas of research such as disease association studies, biomarker discovery and validation, and monitoring of drug treatment regimes.

Accessory Product
The highly unstable nature of RNA transcripts ex vivo creates an even greater challenge for accurate expression analysis. Ongoing metabolic changes can drastically affect gene expression profiles in as little as one hour in standard blood collection tubes (see Figure). Use of the Applied Biosystems® Tempus™ Blood RNA Tubes (SKU #4342792) is therefore recommended. These tubes are designed for direct collection of 3 mL of blood into a plastic, evacuated blood collection tube containing RNA Stabilizing Reagent. The RNA, and therefore the expression profile, is stable in the collection tube for up to five days at room temperature and minimally 7 days at 4°C (see Figure).

Performance with Ease
The ability of the Tempus™ system (Tempus™ Blood RNA Tubes and Tempus™ Spin RNA Isolation Kit) to stabilize RNA expression profiles has been tested over a range of storage conditions (see Figure). The combination provides stable RNA, high yields of purified RNA (observed yields of 6–25 µg from a full blood draw from normal, healthy donors), and high-quality RNA (A260/280 ratio greater than 1.9 and RIN greater than 7.0) suitable for use in downstream applications such as quantitative PCR, microarray profiling, and other RNA analysis methods.

PicoPure™ RNA Isolation Kit Applied Biosystems™

The Arcturus® PicoPure® RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells for use with your gene expression analysis.

Key Product Features:

• Greater sensitivity—isolate RNA from even a single cell
• Superior efficiency—maximize RNA recovery from samples
• Streamlined concentration—elute with small volumes
• Flexible application—compatible with many tube sizes
• Integrated contamination prevention—column lids ensure that samples remain RNase free

Isolate RNA from Even a Single Cell
The PicoPure® RNA Isolation Kit is engineered to recover high-quality total RNA consistently from fewer than ten cells. The high recovery rate attained using this kit is valuable not only for small samples containing picograms of RNA but also for samples containing up to 100 µg of RNA. Since the RNA elutes in as little as 10 µl of buffer, it is ready for gene expression analysis without any vacuum concentration. This kit is available in two sizes, enough for 40 or 200 isolations.

Get Superior RNA Recovery
Small, valuable samples often cannot be replaced. Performing gene expression studies on these precious samples requires getting maximum RNA recovery. Comparative studies show that, with small, RNA-poor cell or tissue samples, the PicoPure® Kit dramatically outperforms other commercial total RNA isolation kits in efficiency.

Eliminate Time-consuming RNA Concentration
The simple isolation protocol consists of extracting cellular RNA, then loading the extract onto the specially designed MiraCol™ Purification Column to bind the RNA. After washing away impurities, the RNA elutes in only 10 µl of buffer, ready for use without vacuum concentration or transfer into a new reaction tube.

High Yield Purification Columns
The PicoPure® RNA Isolation Kit features unique, high-recovery MiraCol™ Purification Columns, engineered to maximize recovery from microscopic samples. The columns have convenient integrated lids to help ensure that samples remain RNase free. In addition, the Kit comes complete with all necessary buffers, an easy-to-follow User Guide, optimized protocols for handling Laser Capture Microdissected (LCM) samples, and protocols for isolating RNA from larger samples.

Deliver RNA Ready for Amplification and Microarray Analysis
RNA isolated with the PicoPure® RNA Kit is ready, without vacuum concentration, for amplification with the RiboAmp® RNA Amplification Kit. The two kits together allow for highly reproducible gene expression results.

Retain Low-abundance mRNA
The PicoPure® RNA Isolation Kit preserves mRNA in all abundance classes through the isolation process, minimizing the loss of important genes of interest. Low, medium, and high-abundance genes are consistently recovered and available for expression analysis.

Maintain High RNA Quality
The PicoPure® RNA isolation process allows for high-quality RNA. Total cellular RNA prepared from LCM samples remains intact after isolation, increasing confidence in subsequent molecular analysis.

iPrep™ PureLink™ Total RNA Kit Invitrogen™

Choose the iPrep™ PureLink® Total RNA Kit for high yields from even the smallest sample (10 mg of tissue, 10e6 cultured cells or 1 ml of blood). The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.

iPrep™ Trizol™ Plus RNA Kit Invitrogen™

Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.

PureLink™ RNA Mini Kit Invitrogen™

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris. The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 µg). This means that users can process both small and large sample sizes with the same RNA isolation kit.

• Process sample inputs from 5–200 mg of animal/plant tissue or from 0.5 x 106 to 108 tissue culture cells with one spin column in 20 minutes
• Isolate more RNA than competitor mini spin columns: up to 1000 µg of total RNA from one column
• Use isolated RNA in most applications without the need for further DNase treatment (very low minimal residual DNA carry over)
• Flexible–optional on-column or post-isolation DNase treatment is available with PureLink DNase Set for easy removal of residual DNA

Extra-large binding capacity enables rapid RNA purification using standard laboratory equipment
The PureLink RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 µg of purified RNA from a single extraction (see Figure 1). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with just trace amounts of residual genomic DNA contamination. The extra-large binding capacity enables one kit to handle most RNA isolation needs in a quick 20 minute protocol without the need for special sample processing instruments.

Easy, optional, on-column DNase treatment for sensitive applications
The PureLink RNA Mini Kit columns are highly efficient for isolating high quality total RNA while removing the majority of genomic DNA. In general, most applications requiring RNA from animal tissue or mammalian cell lines do not require additional DNase treatment. However, some applications such as gene expression analysis by qRT-PCR without intron-spanning primers or working with samples from organisms with very small or no introns may require more complete removal of residual contaminating DNA. The PureLink DNase Set allows for convenient on-column digestion of DNA during the RNA isolation protocol. Treating with DNase while “on-column” is easier and allows higher RNA recovery than treating with DNase after the RNA has been isolated. The PureLink DNase Set can also be used to remove residual DNA from RNA that has been previously purified. Both the “on-column” and post-RNA purification workflows are options available with the PureLink DNase Set.

Simplified, non-toxic RNA purification
The PureLink RNA Mini Kit utilizes non-toxic guanidine-isothiocyanate lysis buffer to protect the RNA during the isolation steps. Special RNase-free reagents combined with certified RNase-free silica membranes in a unique column configuration allow for a safe and easy procedure that can typically be completed in less than 20 minutes without the need of hazardous phenol/chloroform extraction, CsCl centrifugation, or LiCl or alcohol precipitation.

The PureLink RNA Mini Kit is recommended for use with the Homogenizer (Cat. No. 12183026), designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.

For plant tissues rich in polyphenolics or starch (e.g., pine needles, potato tubers), we recommend using PureLink Plant RNA Reagent (Cat. No. 12322012) together with the PureLink RNA Mini Kit.

Elution Buffer for Dynabeads™ mRNA Purification Kits Invitrogen™

This Elution Buffer (10 mM Tris-HCl, pH 7.5) is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61006, 61011, 61012, and 61021. It is made available separately for applications that require more Elution Buffer than is provided in the kit.

ChargeSwitch™ Total RNA Cell Kit Invitrogen™

The ChargeSwitch® Total RNA Cell Kit is ideal for the purification of up to 25 µg of total RNA from up to 1 × 106 cultured mammalian cells or from 1 ml overnight bacterial culture, including full-length RNA and small molecular weight RNA species. This kit offers a unique aqueous-based method for purifying total RNA from cultured mammalian or bacterial cells resulting in exceptionally clean and pure RNA with high integrity (Figure 1). ChargeSwitch® technology eliminates the use of common enzymatic inhibitors such as phenol, chaotropic salts or ethanol, resulting in exceptionally clean RNA. Due to its unique format, this kit also performs exceptionally well at concentrations useful for greater sensitivity in downstream analyses (Figure 3), yielding faster scientific results.

Features of The ChargeSwitch® Total RNA Cell Kit:

• Effectively captures up to 25 µg of total RNA from up to 1 × 106 cultured mammalian cells or 1 ml bacterial culture
• Purifies a wide range of transcripts including increasingly important small RNAs (smaller than 200 nucleotides)
• Provides the ability to purify greater yields of RNA from very small sample sizes
• Eliminates the use of common enzymatic inhibitors providing exceptionally clean RNA
• Results in improved sensitivity in downstream analyses
• Allows flexibility for high-throughput applications

The ChargeSwitch® technology format is scalable and is a highly-sensitive RNA extraction method for successful downstream applications, including gene expression studies, promoter studies, siRNA, gene regulation, transcriptional analysis, RT-PCR, and qRT-PCR. This unique format also allows flexibility for application in high-throughput automation.

How ChargeSwitch® Technology Works:
Based on a patented switchable chemistry, ChargeSwitch® purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge attracting RNA. At higher pH, the surface chemistry is neutral, thereby releasing any attraction to bound RNA (Figure 2).

TaqMan™ Fast Advanced Cells-to-CT™ Kit Invitrogen™

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. TaqMan Fast Advanced Cells-to-Ct cell lysis reagents are supplied with highest sensitivity Fast Advanced reverse transcription enzyme mix, 2x RT buffer, and best-in-class TaqMan Fast Advanced master mix. The TaqMan Fast Advanced Cells-to-Ct Kit gives you all the sensitivity you need in the fastest workflow available: get gene expressions analysis results from an entire plate of cells in about 80 minutes.

Complete—optimized workflow includes cell lysis reagents with gDNA removal, new Fast Advanced RT enzyme mix, buffer, and new TaqMan Fast Advanced Master Mix
Fast—7-minute sample prep, including DNase treatment, at room temperature
Easy—lyse samples in a tube or directly in culture plates
Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Unique method eliminates the need to isolate RNA from cultured cells
The Cells-to-Ct method features a unique lysis reagent that lyses cells and protects RNA. DNA removal occurs simultaneously in the 5-minute lysis step to save time and improve ease of use. Lysis is then stopped by the addition of a “Stop Solution” that irreversibly binds to the lysis reagents.

The TaqMan Fast Advanced Cells-to-CT Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan Fast Advanced Master Mix for real-time PCR analysis. TaqMan primer/probe sets are sold separately.

Simple 7-minute sample preparation
The TaqMan Fast Advanced Cells-to-CT Kit is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for five minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at –20°C for a short term or -80°C for a long term.

Maximize sensitivity of detection
Unlike some competitor kits that limit the amount of lysate in the RT reaction to 10%, the TaqMan Fast Advanced Cells-to-CT Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan Fast Advanced Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

Reduced sample loss and transfer error
Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

Compatible with gold standard TaqMan Gene Expression Assays
The TaqMan Fast Advanced Cells-to-CT Kit workflow enables unsurpassed gene expression evaluation with any of the >700,000 TaqMan Gene Expression Assays. This kit has been extensively tested for specificity with a broad selection of TaqMan Gene Expression Assays.

Proven performance, proven together
All components of the TaqMan Fast Advanced Cells-to-CT Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits. And the TaqMan Fast Advanced Cells-to-CT Kit has been validated with TaqMan Gene Expression Assays for added quality assurance, giving you the sensitivity you need in the fastest sample-to-answer workflow available.

Cells-to-CT™ Bulk Fast Advanced RT Reagents Invitrogen™

Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT kit (20X RT enzyme mix and 20X RT buffer). These reagents are sufficient for 2500 RT reactions. When combined with the Cells-to- CT Bulk Lysis Reagents, these reagents can be easily incorporated into automated, high-throughput applications.

RNAqueous™-Midi Total RNA Isolation Kit Invitrogen™

The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.
• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

GeneJET Stabilized and Fresh Whole Blood RNA Kit Thermo Scientific™

Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Highlights

Versatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ Tubes
Convenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
Efficient—up to 4 µg of total RNA from 500 µL of healthy human blood
Pure—isolates intact total RNA free of any RT-qPCR inhibitors

Applications

Isolated total RNA can be used in:
• RT-PCR
• RT-qPCR
• Northern Blotting

Includes
• Lysis Buffer (R2841)
• Wash Buffer 1 (conc.)(R2851)
• Wash Buffer 2 (conc.)(R2861)
• DNase l (lyophilized)
• DNase I Reconstitution Buffer
• 2x DNase I Buffer
• Manganese Chloride Solution
• Water, nuclease-free
• GeneJET RNA Purification Columns pre-assembled with Collection Tubes
• Collection Tubes, 2 mL
• Collection Tubes, 1.5 mL

Related Products
Lysis Buffer for GeneJET Blood RNA Kit
Wash Buffer 1 for GeneJET Blood RNA Kit (concentrated)
Wash Buffer 2 for GeneJET Blood RNA Kit (concentrated)

RiboMinus™ Transcriptome Isolation Kit, yeast Invitrogen™

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods

• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)

Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.

GeneJET RNA Purification Kit Thermo Scientific™

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

Universal – can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
Efficient – high yields of total RNA.
Fast – 15 minutes after lysis step
Pure – an A260/280 ratio > 1.9
Convenient – spin columns are capped and assembled with collection tubes

Applications

• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assayIncludes Proteinase K Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Collection Tubes) Collection Tubes (2 ml) Collection Tubes (1.5 ml) Detailed Protocol

Dynabeads™ mRNA DIRECT™ Micro Purification Kit Invitrogen™

The Dynabeads® mRNA DIRECT™ Micro Kit is designed for simple and rapid isolation of pure, intact polyadenylated (poly(A)) mRNA. The kit isolates highly purified and intact mRNA directly from cells, tissue, and total RNA samples.

• Obtain extremely pure mRNA in about 15 minutes
• Integrate total RNA purification and mRNA enrichment steps
• Perform reverse transcription and PCR amplification right on the bead
• Use in whole transcriptome library preparation with the Ion Total RNA-Seq Kit v2.0

Rapid Isolation of High Purity mRNA Directly from Samples
The Dynabeads® mRNA Direct Kit contains magnetic beads for the isolation the mRNA transcriptome from a wide variety of samples. Isolation of intact mRNA is possible thanks to RNase inhibitiors in the Lysis/Binding buffer, combined with stringent hybridization and washing steps. Ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are eliminated from the preparation. Only polyadenylated RNA species (mRNA) are captured, resulting in cleaner preparations and more sensitive results. Within minutes, pure mRNA is isolated and ready for use in downstream applications.

A Straightforward Enrichment Procedure
The Dynabeads® mRNA DIRECT™ Micro Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. Superparamagnetic Dynabeads®, coupled to oligo-(dT)25, are first equilibrated with Lysis/Binding Buffer, and then mixed with cell or tissue lysate in order to bind mRNA. The beads are then washed to remove contaminating RNA species, and then mRNA is eluted in as little as 5 µl of 10 mM Tris-HCl. The entire process is facilitated by the use of a neodymium magnet (purchased separately), which allows for the quick and efficient immobilization of the magnetic beads during buffer changes.

Designed for a Broad Range of Sample Types
Nearly any biological sample of vertebrate, invertebrate, plant, fungal, or bacterial origin can be used with the Dynabeads® mRNA DIRECT™ Kit. The kit is also ideal for extracting mRNA and viral polyadenylated RNA from blood, serum and sputum. Nucleic acid preparations, including total RNA and unfractionated nucleic acid samples, as well as formalin-fixed, paraffin-embedded (FFPE) tissues, may also be extracted using the Dynabeads® mRNA DIRECT™ Kit.

RNA is suitable for All Downstream Molecular Applications, Including:
• RNA-sequencing
• gene cloning
• cDNA synthesis, cDNA library construction
• RT-PCR, Quantitative RT-PCR
• RPA - Ribonuclease Protection Assay
• Subtractive Hybridization
• Dot/slot Hybridization
• Primer extension

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

RNAqueous™ Total RNA Isolation Kit Invitrogen™

The RNAqueous® Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous® Kit provides:

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
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