Shop All Plasmid DNA Purification Kits

CloneChecker™ System (for screening bacterial colonies) (Invitrogen™)

CloneChecker™ System is a rapid method for screening recombinant bacterial colonies or liquid cultures of colonies for the presence of target plasmid DNA (1). The CloneChecker™ System:

• Screens bacterial colonies for plasmid DNA and insert of interest prior to purification
• Screens for inserts >200 bp in plasmids up to 15 kb by size comparison to the original vector or a DNA size marker
• Goes from plated colonies through lysis in less than 5 minutes
• Allows for supercoiled, restriction digest, or PCR analysis
• Works with single-copy plasmids starting with a 2-mm bacterial colony
• Includes sufficient reagents for processing 100 bacterial samples by supercoiled analysis (direct size comparison); and 100 samples by restriction endonuclease or PCR analysis

GeneJET Plasmid Midiprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture
High concentration of DNA purified—> 400 ng/ µL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
In vitro transcription
• Transfection of robust cell lines

PureLink™ Pro Quick96 Plasmid Purification Kit (Invitrogen™)

The PureLink® Pro Quick96 Plasmid Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of plasmid DNA from E. coli in as little as 45 minutes (Table 1). Using the PureLink® Pro Quick96 Plasmid Kit, you will:

• Obtain consistently high yields of high-purity plasmid DNA
• Experience ease of use and better performance due to improved plate design and protocol
• Increase processing flexibility with protocols for centrifuge, vacuum manifold, or automated platforms

How it works
The PureLink® Pro Quick96 Plasmid Kit uses two 96-well plates, a filtration plate (clear O-ring), and a binding plate (red O-ring) to purify plasmid DNA. Bacterial cells are subjected to alkaline lysis and are pelleted by centrifugation. The bacterial lysate is then applied to the Quick96 Filtration Plate for clearing and collection in the Quick96 Binding Plate, where the plasmid DNA binds to the silica membrane. After washing steps to remove contaminants, purified DNA is eluted and ready for use in downstream applications. The entire procedure can be completed in 45 minutes (Figure 1).

Improved plate design
The PureLink® Pro Quick96 Plasmid Kit provides optimized semiskirted 96-well plates for improved performance and results (Figure 2). The Quick96 Filtration Plate is designed for fast and efficient clearing of the bacterial lysates. The Quick96 Binding Plate offers an increased binding capacity of up to 20 µg plasmid DNA. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation, or with an automated liquid handling platform. The semiskirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
The PureLink® Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20 µg of plasmid DNA from E. coli strains grown in up to 5 ml LB, 3 ml 2xYT, or 3 ml TB. In an automated platform using 1.5 ml E. coli culture grown overnight in a square-well block, typical yields are 5-15 µg plasmid DNA, with an average of 9.4 µg (Figure 3). The resulting plasmid DNA is supercoiled with no detectable genomic DNA or RNA (Figure 4). The PureLink® Pro Quick96 Plasmid Kit provides higher yields of high-quality plasmid DNA for use in common downstream applications such as automated sequencing, PCR, restriction enzyme digestion, and cloning.

PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit (Invitrogen™)

The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour. Using the HiPure Filter Columns and HiPure Precipitators supplied in the kit, you can purify plasmid DNA without the centrifugation steps required in traditional isolation protocols. The quality of the purified plasmid DNA is equivalent to that obtained by two passes through cesium chloride gradients - the most rigorous method for DNA purification - making it suitable for transfection and other applications requiring high-purity plasmid DNA. The PureLink™ HiPure Plasmid FP Maxiprep Kit (Table 1) provides:
• Simple protocols - bacterial lysate clarification and isopropanol precipitation without centrifugation
• Fast results - from E. coli cell pellet to high-purity, transfection-grade plasmid DNA in about 60 minutes
• High yields - up to 850 µg from a high-copy plasmid

How it works
The PureLink™ HiPure Plasmid FP Maxiprep Kit employs an alkaline lysis method to prepare and rapidly clear bacterial lysates without the need for centrifugation (Figure 1). The unique HiPure Filter Column includes a lysate filtration cartridge integrated into the anion-exchange column for single-step lysate clarification and plasmid DNA binding. Impurities such as RNA, proteins, and other contaminants are removed with a single wash, and ultrapure plasmid DNA is eluted with a high-salt buffer. To precipitate and desalt the DNA, isopropanol is added to the eluted DNA and the mixture is then applied to the HiPure Precipitator using a large syringe. After a subsequent washing and drying step, the plasmid DNA is easily eluted from the HiPure Precipitator with either TE buffer or water. The use of the precipitator eliminates the need for centrifugation, reducing the risk of losing the DNA pellet during supernatant removal, and saving time. The resulting DNA is ready to use in the most challenging applications. The entire protocol - from E. coli cell pellet to ultrapure transfection-grade plasmid DNA - can be completed in about an hour.

Highest yields and ready-to-use concentrations
The HiPure Precipitator has been optimized to allow for lower elution volumes (down to 500 µl) to produce highly concentrated DNA without a significant decrease in yields. In fact, yields at higher concentrations are consistently higher than the competitor kits (Figure 2). Yields and concentration can be adjusted to meet the needs of your downstream application.

GeneJET Plasmid Miniprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

Highlights

Efficient—high yields of up to 20 µg of high quality plasmid DNA
Fast—procedure takes less than 14 minutes
Convenient—no phenol/chloroform extraction or alcohol precipitation required
Pure—purified DNA is immediately ready to use

Applications

• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
In vitro transcription
• Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol

Related Products
GeneJET Plasmid Miniprep Kit
RNase A
Elution Buffer for GeneJET Plasmid Miniprep Kit
Lysis Solution for GeneJET Plasmid Miniprep Kit
Resuspension Solution for GeneJET Plasmid Miniprep Kit
Neutralization Solution for GeneJET Plasmid Miniprep Kit
Wash Solution for GeneJET Plasmid Miniprep Kit
Binding Buffer for GeneJET Plasmid Miniprep Kit

ChargeSwitch™-Pro Plasmid Miniprep Kit (Invitrogen™)

The ChargeSwitch®-Pro Plasmid Miniprep Kit generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. To accomplish this, the ChargeSwitch®-Pro Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method

• Cleaner purified product for improved performance in downstream applications

• Ready-to-use, complete kit for a fast, hassle-free start

ChargeSwitch®-Pro Plasmid Miniprep Kit is ideal for purifying plasmid DNA from 1-5 ml overnight bacterial cultures, yielding up to 20 µg of plasmid DNA.

How it works

The ChargeSwitch® technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch® surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch® protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step (Figure 1, Table 1), making adapting to the ChargeSwitch® method effortless.

Better downstream results
DNA purified using the ChargeSwitch®-Pro Plasmid Miniprep Kit lacks potentially inhibitory compounds, improving DNA integrity for better downstream PCR performance (Figure 2). Expect to see improved cloning due to higher transformation efficiencies (Figure 3), faster restriction enzyme digestions (Figure 4), sequencing reads in excess 800 bp with Phred scores >20 (Figure 5), and a higher degree of supercoiling (Figure 6).

Contents and Storage:
The ChargeSwitch®-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.

PureLink™ Expi Endotoxin-Free Buffer Set (Invitrogen™)

The PureLink Expi Endotoxin-Free Buffer Set provides the same buffers included in the PureLink Expi Endotoxin-Free plasmid purification kits, made available separately here for those applications that would benefit from additional buffers. The Buffer Set includes all buffers and endotoxin-free components needed to enable 30 Maxi, 4 Mega, or 2 Giga PureLink Expi Endotoxin-Free plasmid purifications. Specifically, the buffer set includes RNAse A, resuspension buffer (R3), lysis buffer (L7), precipitation buffer (N3), equilibration buffer (EQ1), wash buffer (W8), elution buffer (E4), TE buffer (TE), endotoxin removal buffer, lysis indicator, and endotoxin-free water.

PureLink™ HQ Mini Plasmid DNA Purification Kit (Invitrogen™)

The PureLink HQ Mini Plasmid Purification Kit is designedfor the isolation of high-quality plasmid DNA that is suitablefor restriction enzyme digestion, PCR, sequencing, bacterialcell transformation, and mammalian cell transfection.Using the kit, plasmid DNA can be isolated from varyingamounts of bacterial cells.

The PureLink HQ Mini Plasmid Purification Kit has thefollowing advantages:
• Higher spin column capacity for plasmid DNA ascompared to other commercially available plasmidpurification systems
• Designed to isolate high-quality plasmid DNA in less thanan hour
• Minimal genomic DNA contamination of the purifiedsample
• Reliable performance of the purified DNA in downstreamapplications

Process overview
Bacterial cells are pelleted, resuspended, and lysed. Thelysate is then neutralized and conditions are adjusted forsubsequent binding. After clarification by centrifugation, thelysate is processed through the PureLink spin column. TheDNA binds to the silica-based membrane in the column, andimpurities are removed by a single wash step. The DNA isthen eluted in Elution Buffer or water.

Chargeswitch™ Pro Filter Plasmid Maxi Kit (Invitrogen™)

The Chargeswitch® -Pro Filter Plasmid Maxi Kit includes 10 plasmid maxipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch®-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification" column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding" column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.



To accomplish this, the ChargeSwitch®-Pro Filter Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units < 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)



How it works
The ChargeSwitch® technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch® surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch® protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch® method effortless.

ChargeSwitch™ NoSpin Plasmid Micro Kit (Invitrogen™)

Sample size 0.5-1 ml overnight culture; Typical yield up to 5 µg

• Provides centrifuge-free purification, ideal for automation
• Avoids introduction of enzymatic contaminants for better results
• Optimized for automation

The ChargeSwitch® NoSpin Kits provide centrifuge-free microprep plasmid purification, ideal for automation. The novel NoSpin reagent causes bacterial cell flocculation and allows cells to be pelleted without the need for automation bottlenecks such as centrifugation. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

GeneJET Plasmid Maxiprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 10 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

Efficient—high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
High concentration of DNA purified—> 400 ng/ µL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
In vitro transcription

MagJET Plasmid DNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Plasmid DNA Kit, sufficient for 96 preps, is designed for fast and efficient purification of plasmid DNA (up to 4-5 µg/mL) or high copy plasmid DNA from overnight E. coli culture.

the kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET Magnetic Beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput users.

The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors. Purified plasmid DNA can be used in a wide range of downstream applications such as PCR, qPCR and other enzymatic reactions.

Highlights

High yields – up to 5 µg of plasmid DNA from 1 mL of bacterial culture
Pure – isolated DNA is immediately ready-to-use in downstream application such as sequencing, transcription, digestion with restriction enzymes or transfection of robust cell lines
Flexible – automation and manual processing protocols available
Repeatable results and robust performance

Applications

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation
• Transfection of robust cell lines

S.N.A.P.™ Plasmid DNA MiniPrep Kit (Invitrogen™)

The S.N.A.P.™ MiniPrep Kit is a plasmid DNA purification system designed to yield pure plasmid DNA (Figures 1 and 2) for automated sequencing in just 25 minutes. Pure plasmid DNA can improve your automated sequencing results by producing:

• Longer reads
• Lower background
• Higher accuracy
• Stronger signal peaks

To ensure the highest quality, each lot of S.N.A.P.™ MiniPrep Kits is used in an automated sequencing reaction. At least 450 bases of 98.5% accurate sequence must be obtained. Typical read lengths are as high as 650 bases. The S.N.A.P.™ MiniPrep Kit yields up to 10 µg of pure miniprep plasmid DNA from 1-3 ml of overnight culture.

PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit (Invitrogen™)

The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 1.5 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

Advantages of the PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit include:
Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
High yields—isolate up to 1.5 mg of high-quality plasmid DNA
Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

PureLink™ HiPure Plasmid Miniprep Kit (Invitrogen™)

The PureLink® HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without the use of any organic solvents or cesium chloride, and contains low endotoxin levels.
• Fast – Highly-quality purified plasmid DNA in under 2 hours
• Efficient – Increased yields with low endotoxin levels

Plasmid DNA of High Purity
The PureLink® HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA from E.coli in 1.5-2 hours. The kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients (Figure 1). The need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials.

High Yield and Efficient Endotoxin Removal
PureLink® HiPure Plasmid Miniprep Kit allows purification of up to 30 µg of high quality plasmid DNA from 1-3 ml overnight E.coli cultures in approximately 1 hour, with an endotoxin level of 0.1-1 EU/ µg, when cloning high copy number plasmids (Table 1). Typically, DNA isolated using the PureLink® HiPure Plasmid Miniprep Kit has an A260/A280 > 1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a Number of Downstream Applications:
The purified DNA is ultrapure and suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections (Figure 2), automated and manual DNA sequencing, PCR amplification, in vitro transcription, bacterial cell transformation, cloning, and labeling.

For Research Use Only. Not intended for diagnostic procedures.