Shop All Genomic DNA Purification Kits

MagMAX™ DNA Multi-Sample Ultra 2.0 Wash I Solution (Applied Biosystems™)

This Wash I solution is included in the MagMAX DNA Multi-Sample Ultra 2.0 Kit. It is available separately for applications that require more Wash Isolution than is provided in the kit.

GeneJET Plant Genomic DNA Purification Kit (Thermo Scientific™)

Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.

Highlights

Fast—procedure completed in 30 min following cell lysis
Convenient—protocol does not include clogging-prone lysate filtration step
High yield—up to 32 µg of high quality DNA from 100 mg sample, including nuclear, mitochondrial, and chloroplast DNA
Compatible—use with awide variety of samples (leaves, roots, sprouts and other parts of various plants)

The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (e. g. young wheat leaves) is 30 to 32 µg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions.

Applications

• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
FastDigest and conventional restriction digestion
• Southern blotting

MagMAX™ DNA Multi-Sample Ultra 2.0 Lysis/Binding Solution (Applied Biosystems™)

This Lysis/Binding solution is included in the MagMAX DNA Multi-Sample Ultra 2.0 Kit. It is available separately for applications that require more Lysis/Binding solution than is provided in the kit.

PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K (Applied Biosystems™)

The PrepSEQ® Rapid Spin Sample Preparation Kit with Proteinase K is recommended for difficult to lyse bacteria like Listeria monocytogenes. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.


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For Research Use Only. Not for use in diagnostics procedures.

MagMAX™ DNA Multi-Sample Ultra 2.0 Elution Solution (Applied Biosystems™)

This Elution solution is included in the MagMAX DNA Multi-Sample Ultra 2.0 Kit. It is available separately for applications that require more Elution solution than is provided in the kit.

ChargeSwitch™ gDNA Normalized Buccal Cell Kit (Invitrogen™)

The ChargeSwitch® gDNA Normalized Buccal Cell Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from human buccal swabs in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Ideal for STR profiling
• Compatible with high-throughput protocols

Rapid Purification Protocol
The ChargeSwitch® gDNA Normalized Buccal Cell Kit allows purification of genomic DNA from human buccal cell swabs or pelleted cells from a mouthwash in less than 15 minutes after lysates preparation. ChargeSwitch® gDNA Normalized Buccal Cell Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. High-quality genomic DNA is purified at a normalized concentration of 1-3 ng/ µl in a total volume of 150 µl (Figure 1) by eliminating the need for lengthy quantitation and dilution steps. Samples can be stored for up to 2 weeks at 4°C before processing without a noticeable loss in DNA yield or quality.

Note: DNA yield varies and is dependent on several factors including the technique of the person taking the swab, whether the donor is a high or low shedder, and the type of swab used.

Validated for Single Tandem Repeat (STR) Profiling
After employing the ChargeSwitch® gDNA Normalized Buccal Cell Kit, purified gDNA is suitable for use in STR analysis of poor or environmentally degraded samples, and for downstream applications such as PCR.

Compatible with Single Tube and High-Throughput Protocols
The ChargeSwitch® gDNA Normalized Buccal Cell Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.

For processing up to 24 individual samples at a time the MagnaRack™ two-piece magnetic separation tube rack is required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.

For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator is required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.

For Research Use Only. Not intended for diagnostic procedures.

ChargeSwitch™ gDNA Rendered Meat Purification Kit (Invitrogen™)

The ChargeSwitch® gDNA Rendered Meat Purification Kit allows for rapid and efficient purification of genomic DNA (gDNA) from animal feed, animal meal, and heparin products for downstream molecular analysis. The kit is listed in the U.S. Food and Drug Administration Center for Veterinary Medicine’s protocols as a sample preparation method suitable for monitoring crude heparin quality and testing animal feed for banned materials such as specific animal proteins.

Note: The number of reactions possible with this kit is dependent upon the starting sample type: animal feed & meal samples = 50 preparations, heparin products = 25 preparations.

The ChargeSwitch® gDNA Rendered Meat Purification Kit protocol starts with prepared lysates and takes approximately 15 minutes to complete without centrifugation, vacuum, or organic solvents (for batches of up to 5 samples per operator—larger batch sizes will require longer processing times). The kit employs a magnetic bead with a switchable surface. Low pH conditions cause binding of DNA to the bead while high pH conditions cause the release of the DNA.

Tested by the U.S. Food and Drug Administration
The ChargeSwitch® gDNA Rendered Meat Purification Kit has been thoroughly tested by the U.S. Food and Drug Administration for processing up to 500 mg of animal feed, 250 mg of animal meal, and 250 mg of heparin product per reaction.

References
Development of a Multiplex Real-Time PCR Assay for the Detection of Ruminant DNA in Raw Materials used for Monitoring Crude Heparin for Quality
www.fda.gov/AnimalVeterinary/ScienceResearch/ToolsResources/ucm350289.htm

Development, evaluation, and peer verification of a rapid real-time PCR method for the detection of animal material. Journal of Food Protection, Vol. 72, No. 11, 2009, Pages 2368–2374. U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland 20708, USA.

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K (Applied Biosystems™)

The PrepSEQ® Spin Sample Preparation Kit – Extra Clean with Proteinase K is recommended for difficult to lyse bacteria, like Listeria monocytogenes, that also has a high lipid content, such as soft cheeses. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit – Extra Clean with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.


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For Research Use Only. Not for use in diagnostics procedures.

JetQuick™ Blood and Cell Culture DNA Midiprep Kit (Invitrogen™)

The JetQuick™ Blood and Cell Culture DNA Midiprep Kit is designed for rapid and efficient purification of genomic DNA (gDNA) from blood, body fluids (amniotic fluid, saliva, sperm, lymph), and mammalian cells. It utilizes silica membrane-based technology that allows for isolation of 20-50 kb DNA that is suitable for a wide range of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.

Key benefits include:
• Large volume processing, up to 3 mL of blood or saliva
• Long double-stranded DNA (20-50 kb) recovery
• Compatible with centrifugation or a vacuum manifold

The midiprep format enables the processing of up to 3 mL of whole blood or other body fluid. DNA from up to 1 x 108 mammalian cells can be isolated. The purified DNA is ready for downstream analysis.

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean (Applied Biosystems™)

The PrepSEQ™ Rapid Spin Sample Preparation Kit – Extra Clean is recommended for foods with a high lipid content, such as infant formula and chicken wing samples. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit – Extra Clean meets this need.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.

For Research Use Only. Not for use in diagnostics procedures.

PureLink™ Pro 96 Genomic DNA Purification Kit (Invitrogen™)

The PureLink® Pro 96 Genomic DNA Purification Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types in a high-throughput platform. This single kit allows genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, using a familiar silica semi-skirted 96-well plate format. Advantages of the PureLink® Pro 96 Genomic DNA Purification Kit:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—excellent A260/A280 and A260/A230 absorbance ratios, regardless of sample source
Improved design—optimized, semi-skirted, 96-well plates compatible with centrifugation and most benchtop and robotic vacuum manifolds

Multiple sample sources, one kit
The PureLink® Pro 96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Examples of sample sources and amounts that can be used with the PureLink® Pro Genomic DNA Purification Kit include mammalian cells and tissue, FFPE tissue, Oragene™-preserved saliva, mouse and rat tails, non-nucleated whole blood (human, mouse), nucleated whole blood (bird), blood spot on paper, bacterial cells, and yeast cells.

Improved plate design
The PureLink® Pro 96 Genomic DNA Purification Kit provides optimized, semi-skirted, 96-well plates (see figure), for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. Sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at ~2 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
The PureLink® Pro 96 Genomic DNA Purification Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (see figure). You are also able to get greater yields of higher-purity gDNA (determined by A260/A280 measurements) no matter what your sample type is; from bacteria to tissues to blood to cells (see figures). The enhanced PureLink® Lysis and Wash Buffers help consistently deliver purified gDNA samples with absorbance reading ratios of ~1.8 for both A260/A280 and A260/A230, indicating no contamination carryover of protein or guanidine (see figure).

MagMAX™ DNA Multi-Sample Ultra 2.0 Enhancer Solution (Applied Biosystems™)

This Enhancer solution is included in the MagMAX DNA Multi-Sample Ultra 2.0 Kit. It is available separately for applications that require more Enhancer solution than is provided in the kit.

ChargeSwitch™ gDNA Buccal Cell Kit (Invitrogen™)

Sample size 1 buccal swab; Typical performance up to 6 µg

• Maximum yield sensitivity
• Rapid single-tube protocol, suitable for automation
• High quality DNA for downstream Single Tandem Repeat (STR) analysis

The ChargeSwitch® gDNA Buccal Cell Kit maximizes the yield of high quality genomic DNA from buccal samples for rapid buccal swab processing. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

ChargeSwitch™ gDNA Mini Tissue Kit (Invitrogen™)

The ChargeSwitch® gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance

High Quality, High Yield Plasmid DNA
The ChargeSwitch® gDNA Mini Tissue Kit allows purification of genomic DNA from 10-25 mg quantities of tissue typically in less than 15 minutes following sample preparation and lysis. ChargeSwitch® gDNA Mini Tissue Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 30 µg of gDNA from 0.5 cm mouse tail tips or approximately 25 mg of tissue. For spleen tissue, reduce tissue size to 10 mg.

Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch® gDNA Mini Tissue Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy "on the magnet" and "off the magnet" sample processing.

Improved Performance in Demanding Downstream Applications
The ChargeSwitch® gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.

For Research Use Only. Not intended for diagnostic procedures.

MagMAX™ DNA Multi-Sample Ultra Kit (Applied Biosystems™)

The MagMAX™ DNA Multi-Sample Ultra Kit is optimized to isolate genomic DNA (gDNA) from a variety of samples, such as whole blood, buccal cells, saliva, urine, blood cards, mouth rinse, and tissue. gDNA purified with the kit is ideal for qPCR applications on sensitive, scalable platforms such as the QuantStudio™ 12K Flex System with OpenArray® Block (with AccuFill™ System). The magnetic bead–based purification format allows you to easily scale from processing 12 to 500 samples a day, making it the ideal choice for pharmacogenomics (PGx) studies.

Features of the MagMAX™ DNA Multi-Sample Ultra Kit include:
• Streamlined protocols for numerous non-invasive biological samples
• Optimized to provide DNA yields suitable for OpenArray® and Ion AmpliSeq™ applications
• Packaged for high-throughput needs

One Kit for a Diverse Set of Sample Types
Why purchase multiple purification kits to isolate gDNA from different sample types? The MagMAX™ DNA Multi-Sample Ultra Kit eliminates the headache of ordering these extra kits because it has been optimized for use with a variety of biological samples. The kit is designed for high-throughput isolation of gDNA from 1 buccal swab, 50 to 350 µL of whole blood, up to 400 µL urine (without sample concentration), up to 150 µL saliva, 1-5 mL mouth rinse, blood spot cards, and mammalian tissue samples (up to 10 mg). The protocols and magnetic bead format make the kit fully amenable to automation.

*Please note that the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor with a 24 Deep-well Head is recommended for sample volumes greater than 50 µL.

Benefits of MagMAX™ Magnetic Bead–based Purification
Magnetic beads offer many benefits compared to other technologies for isolating gDNA. Beads bind gDNA more efficiently than glass fiber filters, resulting in higher and more consistent gDNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Accessory Products
Proteinase K (A25561), DNA Binding Beads (A25562), Wash Solution 1 Concentrate (AM8504), and Wash Solution 2 Concentrate (AM8640) are components of the kit that may also be purchased separately.