Shop All Real-Time PCR and Endpoint RT-PCR Kits

TaqPath™ ProAmp™ Master Mix Applied Biosystems™

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Master Mix is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

Request an evaluation sample ›

Two TaqPath ProAmp Master Mix formulations for different throughput needs
• TaqPath ProAmp Master Mix—includes ROX passive reference dye for lower multiplexing of up to three targets
TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE passive reference dye for higher multiplexing of up to four targets

Benefits of TaqPath ProAmp Master Mix
Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock Taq DNA hot-start mechanism
Multiplexing breadth—two formulations that enable single to four-target detection per reaction
Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mix has been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. The figure below shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan genotyping assays using crude lysate from blood samples. TaqPath ProAmp Master Mix has been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High accuracy copy number quantitation
The versatile TaqPath ProAmp Master Mix can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. The figure below shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp Master Mix enables determination of copy number accurately in samples with up to four copies.

General purpose reagents
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled 'For Laboratory Use.'

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›

* Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.

CellsDirect™ One-Step qRT-PCR Kit Invitrogen™

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features of the CellsDirect™ One-Step qRT-PCR Kit include:

Efficiency—offers significant time and cost savings for increased throughput
Convenience—enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
Sensitivity—no separate RNA isolation step increases sensitivity by minimizing sample loss
Broad detection range—detect targets from samples ranging from a single cell to 10,000 cells
Versatility—compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline your workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for your qRT-PCR application
CellsDirect™ technology is validated for gene expression studies involving limited samples. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (see figure). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.

CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan® or LUX™ fluorogenic primers).

Note: This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit (11754-500) with ROX™ Reference Dye included in the 2X Reaction Mix is also available.

TaqPath™ ProAmp™ Multiplex Master Mix Applied Biosystems™

TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Multiplex Master Mix is manufactured under a strong ISO13485 quality management system and designed to produce consistent product performance lot after lot.

Request an evaluation sample ›

Two TaqPath ProAmp Master Mix formulations for different throughput needs
TaqPath ProAmp Master Mix—includes ROX passive reference dye for lower multiplexing of up to three targets
• TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE passive reference dye for higher multiplexing of up to four targets

Benefits of TaqPath ProAmp Master Mix
Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock Taq DNA hot-start mechanism
Multiplexing breadth—two formulations that enable single to four-target detection per reaction
Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mix has been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. The figure below shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan genotyping assays using crude lysate from blood samples. TaqPath ProAmp Master Mix has been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High accuracy copy number quantitation
The versatile TaqPath ProAmp Master Mix can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. The figure below shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp Master Mix enables determination of copy number accurately in samples with up to four copies.

General purpose reagents
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled 'For Laboratory Use.'

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›

* Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.

TaqMan™ Universal Master Mix II, with UNG Applied Biosystems™

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer components

Note: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

ABsolute QPCR Mix, no ROX Thermo Scientific™

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Highlights

• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

Applications
• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

QuantStudio™ Digital PCR Kit Applied Biosystems™

QuantStudio™ Digital PCR Kits enable the accurate and sensitive quantitation of materials beyond the limits of conventional PCR. The kits include QuantStudio™ Digital PCR Plates and 2X TaqMan® Digital Master Mix. The QuantStudio™ Digital PCR Kits are intended for use with our QuantStudio™ 12K Flex System, which enables over 12,000 digital PCR reactions to be run simultaneously to generate up to 48,000+ digital data points per day.

Key Product Features:

• Accurate and sensitive—Detect and count individual molecules to quantify viral load, gDNA, cDNA, plasmids, or next generation sequencing libraries
• Fast—produce up to 192 digital answers per ~2 hour run
• Flexible—Use your existing assays to test from one to 48 assay/sample dilutions per plate
• Wide dynamic range—By accommodating as few as 64 data points per replicate group, a dilution series can easily be loaded into the QuantStudio™ Digital PCR Plate, greatly expanding the range of sample concentrations that can be analyzed to produce a digital answer
• Intuitive and economical—Meet the needs of both your qPCR and dPCR studies on a single unified instrument platform

Simple Workflow
QuantStudio™ Digital PCR Plates are delivered pre-treated with hydrophilic and hydrophobic coatings to accept your own TaqMan® assays. Simply combine the assays with sample and master mix, load onto the QuantStudio™ plate, seal, cycle, and image. The QuantStudio® 12K Flex System's simple workflow enables one person to generate over 48,000 digital data points in one day without the use of additional robotics.

Flexible Formats
Each QuantStudio™ Digital PCR Plate contains 3,072 through-holes arranged in 48 subarrays of 64 through-holes each. The OpenArray® AccuFill™ System and open plate format enables the loading of anywhere between one and 48 samples or sample dilutions per plate dependent upon experimental needs.

A Complete Digital PCR Solution
The QuantStudio™ Digital PCR solution is an affordable, whole-system solution for numerous Digital PCR applications. The decrease in reagent use afforded by this unique combination of technologies greatly decreases the price per data point, allowing more samples to be run for a lower cost of consumables. QuantStudio™ Digital PCR Plates, TaqMan® Digital PCR Master Mix, QuantStudio™ accessories, and DigitalSuite™ PCR Software have been designed to work together to enable you to obtain accurate and reproducible Digital PCR results.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Fast SYBR™ Green Master Mix Applied Biosystems™

Fast SYBR® Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR® Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR® Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.

TaqMan™ Advanced miRNA Human B 96-well Plates, standard Applied Biosystems™

The Applied Biosystems TaqMan Advanced miRNA Human B 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human B 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA B Cards v3.0.

TaqMan™ Multiplex Master Mix Applied Biosystems™

Applied Biosystems® TaqMan® Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (<40 minutes) with excellent concordance of results in singleplex and multiplex reactions. TaqMan® Multiplex Master Mix contains AmpliTaq® Fast DNA PolymeraseUracil-N Glycosylase (UNG), dNTPs with dUTP, MUSTANG PURPLE® dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

Features of TaqMan® Multiplex Master Mix include:

• Optimized for multiplexing—validated for quadplex gene expression and genotyping assays
• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity
• Reduced run times on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
• Seamless integration into your experiments—validated with Applied Biosystems® TaqMan® assays and TaqMan® QSY® probes for gene expression and genotyping experiments
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility

Optimized for Multiplexing
Confidence is paramount when it comes to your results. TaqMan® Multiplex Master Mix is designed to deliver accurate results for multplex reactions and ensure excellent concordance of results in both singleplex and multiplex reactions.

Best-in-class Performance
TaqMan® Multiplex Master Mix is designed for performance equal to or better than other multiplex master mixes on the market. TaqMan® Multiplex Master Mix has been benchmarked against the leading suppliers' multiplex master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity.

Reduced Run Times on Standard Instrumentation
TaqMan® Multiplex Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides.

Carry-over Contamination Control
TaqMan® Multiplex Master Mix contains heat-labile uracil-DNA glycosylase (UDG) for worry-free carryover contamination control. The dUTP in the mix ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs. A UDG incubation step before PCR cycling destroys any contaminating dU-containing product from previous reactions. UDG is then inactivated by the high temperatures during normal PCR cycling, thereby allowing the amplification of genuine target sequences.

Instrument Compatibility
TaqMan® Multiplex Master Mix can be used in fast cycling mode on both standard and fast qPCR instruments, such as: 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System, ViiA™ 7 Real-Time PCR System, and QuantStudio™ 6 Flex, 7 Flex, and 12K Flex Real-Time PCR systems.

SYBR™ Green PCR Master Mix Applied Biosystems™

Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• Premixed components stored at 2–8°C significantly reduce assay setup time
• SYBR® Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience
Applied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan® probes are required. SYBR® Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR® Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

QuantStudio™ 3D Digital PCR Starter Kit Applied Biosystems™

The QuantStudio™ 3D Digital PCR Starter Kit is designed to confirm the performance of the QuantStudio™ 3D Digital PCR System either at the time of system installation or as part of future, user-defined system verification procedures using established Applied Biosystems™ consumables, reagents, control DNA, and reference assays. The Starter Kit may also be used for training users on the QuantStudio 3D Digital PCR System workflow.

The Starter Kit contains the QuantStudio™ 3D Digital PCR 20K Chip Kit v2 and QuantStudio™ 3D Digital PCR Reagent Kit to provide a complete set of starter reagents and consumables.

Protein Thermal Shift™ Dye Kit

Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.

Save Time and Money with Your Sample Screening
This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR systems enable researchers to inexpensively and efficiently:

• Screen samples for relative protein thermal stability
• Screen molecules (e.g. small molecule drug candidates, fragment-based libraries, and antibodies) for ligand binding to proteins in a high-throughput manner
• Screen samples for stability changes after protein point mutations or modifications
• Systematically identify suitable⁄optimal buffer conditions for the measurement of protein-ligand interactions, production of stable protein preparations, protein crystallization, and storage of proteins
• Quickly QC protein preps to ensure protein integrity and⁄or purity

Two Kits to Choose From
The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 2000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.

Simplify Analysis with Protein Thermal Shift™ Software
Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Advanced miRNA Human Serum/Plasma 96-well Plates, standard Applied Biosystems™

The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced Serum/Plasma 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 2
Number of unique miRNAs (not including exogenous controls): 188
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

TaqMan™ Universal Master Mix II, no UNG Applied Biosystems™

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Optimized buffer components

Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping Assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

ABsolute QPCR Mix, ROX Thermo Scientific™

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Highlights

• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

Applications
• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green
Results per page
    spinner