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Maxima Probe/ROX qPCR Master Mix (2X) (Thermo Scientific™)

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima Probe/ROX qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima Probe/ROX qPCR Master Mix (2X)
Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/Fluorescein qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with fluorescein passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Single Cell-to-CT™ qRT-PCR Kit (Invitrogen™)

The Ambion® Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells. Each kit contains reagents for sample preparation, reverse transcription, pre-amplification, and qPCR that have been optimized together in a simple workflow that can be completed in only 5 steps (see figure).

Key product features:

• Pre-optimized workflow for real-time RT-PCR from single cells
• Maximum sensitivity for single-cell analysis
• Superior performance and reproducibility compared to alternative methods
• Complete kit convenience and easy-to-follow protocol

Pre-optimized protocol ensures success and saves time
Each workflow begins with a simple 7-minute sample prep, where cells are effectively and reproducibly lysed with minimal processing into a solution that is compatible with downstream RT-PCR, without the need for purification. This is followed by the use of Superscript® RT for reverse transcription, the TaqMan® PreAmp Master Mix to amplify the cDNA, and TaqMan® Gene Expression Master Mix for qPCR. All four of these steps have been developed to provide maximum sensitivity. The entire cell sample is maintained in the same well throughout the procedure so there is no sample loss or dilution of the precious limited material that could affect sensitivity.

Single cell sensitivity
Sensitivity of real-time PCR results can be affected by the efficiency of sample preparation, reverse transcription, or amplification. The Single Cell-to-CT™ Kit has addressed each of these potential problem areas to create a solution that enables reliable and robust gene expression analysis from single cells with maximal sensitivity. Detection of single cell equivalents using the Single Cell-to-CT™ Kit demonstrates appropriate linearity and sensitivity of qPCR results compared to a 100 cell sample control with excellent technical reproducibility (see figure). As expected, individual single cells exhibit greater variability due to inherent biological heterogeneity. In addition, through inclusion of a cDNA pre-amplification step, targets of interest are accurately amplified prior to real-time PCR. This critical step boosts signal in an unbiased manner, extending the analysis potential of limited samples. The accuracy and signal enhancement of a 96-gene panel using pre-amplification is shown (see figure).

Superior performance and reproducibility compared to alternative methods
Traditional sample preparation methods using organic solvents, glass fiber filters, or magnetic beads are not suitable for single-cell analysis due to the loss of sample through incomplete RNA precipitation, binding, and elution. Additionally, most single-cell homebrew methods which involve simple boiling to lyse cells lack the ability to inactivate endogenous RNases to stabilize gene expression profiles and can result in chemical cleavage of the RNA and inhibitor carryover, including gDNA contamination, which can affect all qRT-PCR applications. In contrast, the Single Cell-to-CT™ Kit shows superior sensitivity and reproducibility for single-cell analysis compared to traditional purification or homebrew boiling methods (see figure).

TaqMan™ Advanced miRNA Human A 96-well Plates, fast (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human A 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human A 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA A Cards v2.0.

Platinum™ SYBR™ Green qPCR SuperMix-UDG (Invitrogen™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Platinum SYBR Green qPCR SuperMix-UDG and added additional capabilities for your gene expression analysis.

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Platinum® SYBR® Green qPCR SuperMix-UDG provides a sensitive and convenient system for real-time PCR detection with SYBR® Green I dye. The SuperMix format combines amplification and detection reagents into a ready-to-go mix to make set-up easy and ensure reproducible results. Platinum® SYBR® Green qPCR SuperMix-UDG offers:

–Platinum® Taq DNA Polymerase for sensitive amplification from as few as 10 copies of target (Figure 1)

–100% dUTP and UDG enzyme for superior control of carryover contamination, saving time by reducing the number of failed experiments
–Separate tubes of ROX reference dye and BSA for easy optimization on 96-well plates and glass capillary tube instruments (Figure 2)
–SYBR® Green I dye for simple and easy detection of real-time product

TaqMan™ Fast Advanced Master Mix (Applied Biosystems™)

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It provides best in class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

Features of the TaqMan Fast Advanced Master Mix include:
Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
Reduced run times (<40 minutes) on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
Seamlessly transitions into your experiments—validated with TaqMan assays for gene expression and microRNAs, and TaqMan array micro-fluidic cards

Best-in-class performance
TaqMan Fast Advanced Master Mix has been designed to provide performance equal to or better than standard master mixes. It has been benchmarked against the leading suppliers' standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. TaqMan Fast Advanced Master Mix’s dynamic range (up to 7 logs) is the widest in the industry. The impressive sensitivity of the mix is showcased in the figure below which compares the Ct values of TaqMan Fast Advanced Master Mix and TaqMan Universal PCR Master Mix across a panel of gene expression assays.

Benchtop stability for high-throughput handling and convenience
TaqMan Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. The figure below shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.

For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running plates upon assembly.

Optimized for multiplexing
We realize that confidence is paramount when it comes to your results. For added confidence in the results in every well, TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). The figure below shows results for the experimental target β-actin (gene name: ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.

Validated for microRNA assays
TaqMan Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The data in the figure below demonstrate excellent PCR linearity over a 6-log range of input template.

Reduced run times on standard instrumentation
TaqMan Fast Advanced Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides. The figure below showcases the impressive results achieved when using TaqMan Fast Advanced Master Mix under fast thermal cycling conditions on the Applied Biosystems 7300 Real-Time PCR System. The mix has been tested with all Applied Biosystems standard real-time PCR instrumentation (7900HT, 7500, and 7300 systems) to enable success independent of whether or not you own a fast-enabled instrument.

QuantStudio™ 3D Digital PCR 20K Chip Kit v2 (Applied Biosystems™)

The Applied Biosystems™ QuantStudio™ 3D Digital PCR 20K Chip Kit v2 is a key component of the QuantStudio™ 3D Digital PCR System. Manufactured from a silicon substrate and etched with 20,000 consistently-sized nanoscale reaction wells, the chip enables precise sample loading with an uncomplicated workflow.

The QuantStudio 3D Digital PCR 20K Chip Kit v2 enables improved UV adhesive-free sealing. It requires use of the QuantStudio 3D™ Digital PCR Master Mix v2 and is fully-compatible with all QuantStudio 3D™ Digital PCR Instrument hardware and software.

The QuantStudio 3D Digital PCR 20K Chip Kit v2 is designed to deliver accurate, sensitive, and precise digital PCR data on QuantStudio 3D Digital PCR instruments when combined with associated reagents and QuantStudio™ 3D Analysis Suite™ software.

TaqMan™Gene Expression Assay (FAM) (Applied Biosystems™)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features of TaqMan Gene Expression assays include:
Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
Accurate—identify small fold-changes with high accuracy of quantitation
Extensive content—Over 1.8 million predesigned assays available for over 25 different species
Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
Fast delivery—your assays shipped in as little as 1–2 days (see below)

Approximate ship time
Inventoried: 1–2 days in North America, 3–5 days in Europe*
Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.**

Recommended master mix: TaqMan Fast Advanced Master Mix

*Our most popular assays are stocked in North America and Europe for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.

**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

TaqPath™ qPCR Master Mix, CG (Applied Biosystems™)

TaqPath qPCR Master Mix, CG, is designed for reliable, accurate quantification in gene expression and miRNA workflows. The master mix delivers confident results in both single and duplex reactions, even in the presence of inhibitors commonly found in clinical samples. All lots are functionally tested to help ensure lot-to-lot reproducibility for Ct consistency and dynamic range across a wide variety of assays. This General Purpose Reagent is manufactured according to applicable CFR requirements and labeled "For Laboratory Use". With stringent quality and premier performance, TaqPath qPCR Master Mix is a superior choice for your diagnostic testing or development needs.

Features of the TaqPath qPCR Master Mix include:
• Efficient and linear detection of up to eight logs with gene expression or miRNA assays*
• Enables reliable detection of low-copy templates with reproducible CT results
• Robust multiplexing performance with exogenous or endogenous targets
• Manufactured with stringent production and process controls to help ensure lot-to-lot consistency
• Labeled "For Laboratory Use"

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Validated with a breadth of workflows
TaqPath qPCR Master Mix, CG, has been validated to provide high specificity and dynamic range for use in multiple real-time PCR applications. The formulation can be run in either fast or standard cycling conditions on a wide variety of qPCR platforms. The figure below demonstrates the excellent PCR linearity over an 8-log input range of template with both gene expression and miRNA assays.

In addition, TaqPath qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate (figure below). TaqPath qPCR Master Mix has been benchmarked against similar competitor master mixes and demonstrates equivalent or better sensitivity and dynamic range across a variety of targets (figure below).

Reproducible sensitive detection
We understand the importance of reliably detecting low-copy targets for your test quality and data interpretation. TaqPath qPCR Master Mix, CG, helps generate significant and reproducible CT values for ≤10 copy detection. The figure below shows the consistent CT results obtained from three unique lots when detecting 10-copy inputs. This lot-to-lot CT consistency is preserved across multiple assays with different attributes and expression levels to maximize confidence in your results (figure below).

Optimized for multiplexing
Simultaneous amplification of multiple assays can be beneficial not only as a control to normalize and detect anomalies in experiments, but also to improve efficiency and cost-savings for labs. TaqPath qPCR Master Mix has been optimized with enzymes and component concentrations to facilitate multiplexing while preserving specificity, and it is validated for duplex performance in each manufacturing lot (figure below).

Inhibitor tolerant
Unlike other master mixes on the market, TaqPath qPCR Master Mix’s unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance to inhibitors compared to competitor mixes (figure below).

Manufacturing production and process controls
TaqPath qPCR Master Mix is manufactured under a quality management system that utilizes traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot.

General purpose reagent
For customers in the USA, TaqPath 1-Step RT-qPCR Master Mix is a General Purpose Reagent and "For Laboratory Use".

*Dynamic range is a property of both the assay and template concentration in the sample, as well as in the master mix, thus individual results may vary.

ABsolute Blue QPCR Mix, SYBR Green, low ROX (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

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Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Includes
• ABsolute Blue qPCR SYBR Green low ROX Mix contains 2X master mix supplemented with low ROX reference dye concentration

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

VetMAX™ PRRSV NA & EU Reagents (Applied Biosystems™)

Note: The name of this product has changed. It was previously called PRRSV REAGENTS W/PATCH EACH.

VetMAX™ NA and EU PRRSV Reagents contains primers/probes that enable multiplex, one-step, reverse transcription and real-time PCR (RT-PCR) targeting North American and European Porcine Reproductive and Respiratory Syndrome Virus (PRSSV) RNA and Xeno™ RNA Control. VetMAX™ NA and EU PRRSV Reagents contains an updated primer/probe mix compared to the similar TaqMan® NA and EU PRRSV Reagents.

• Rapid and cost-effective method for amplification of target sequences
• Quality reagents, manufactured under ISO 9001, providing confidence in the supply and quality of products
• Over 20 years in the DNA/RNA arena, with extensive bioinformatics capabilities and experience

PRSSV causes significant losses in the swine industry due to reproductive disorders, respiratory conditions, and growth retardation. PRRSV can be detected in blood and semen 24–48 hours after infection. Real-time PCR is the most sensitive method for monitoring RNA amplification.

TaqMan™ Advanced miRNA Human B 96-well Plates, fast (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human B 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human B 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA B Cards v3.0.

TaqPath™ ProAmp™ Multiplex Master Mix (Applied Biosystems™)

TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Multiplex Master Mix is manufactured under a strong ISO13485 quality management system and designed to produce consistent product performance lot after lot.

Request an evaluation sample ›

Two TaqPath ProAmp Master Mix formulations for different throughput needs
TaqPath ProAmp Master Mix—includes ROX passive reference dye for lower multiplexing of up to three targets
• TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE passive reference dye for higher multiplexing of up to four targets

Benefits of TaqPath ProAmp Master Mix
Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock Taq DNA hot-start mechanism
Multiplexing breadth—two formulations that enable single to four-target detection per reaction
Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mix has been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. The figure below shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan genotyping assays using crude lysate from blood samples. TaqPath ProAmp Master Mix has been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High accuracy copy number quantitation
The versatile TaqPath ProAmp Master Mix can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. The figure below shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp Master Mix enables determination of copy number accurately in samples with up to four copies.

General purpose reagents
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled "For Laboratory Use."

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›

* Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.

QuantStudio™ 12K Flex OpenArray™ Gene Expression Starter Kit (Applied Biosystems™)

A variety of starter kits are available to introduce you to the various applications of the QuantStudio™12K Flex OpenArray® sample block. Each starter kit contains 2 arrays, plus sufficient reagents and accessories to perform two experiments. The Gene Expression starter kit contains 2 gene expression endogenous control plates. The Gene Expression kit also contains cDNA controls to serve as samples for your experiments. Note that the QuantStudio™12K Flex Software (included with the QuantStudio™ 12K Flex System) offers example experiment files for each application.

The accessories provided in the kit include: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.

QuantStudio™ 3D Digital PCR 20K Chip Kit v2 and Master Mix (Applied Biosystems™)

The Applied Biosystems™ QuantStudio™ 3D Digital PCR 20K Chip Kit v2 and QuantStudio™ 3D Digital PCR Master Mix v2 deliver improved and UV adhesive-free sealing. They are fully-compatible with all QuantStudio 3D™ Digital PCR Instrument hardware and software.

The QuantStudio™ 3D Digital PCR 20K Chip Kit v2 is a key component of the QuantStudio™ 3D Digital PCR System. Manufactured from a silicon substrate and etched with 20,000 consistently-sized nanoscale reaction wells, the chip enables precise sample loading with an uncomplicated workflow. It requires use of the QuantStudio 3D Digital PCR Master Mix v2.

QuantStudio 3D Digital PCR Master Mix v2 is specifically formulated with all reagents required to help ensure robust PCR amplification of single copy templates. It is optimized for use with isolated DNA or cDNA, TaqMan™ assays, and the QuantStudio 3D Digital PCR 20K Chip v2.

The QuantStudio 3D Digital PCR 20K Chip Kit v2 and QuantStudio 3D Digital PCR Master Mix v2 are designed to deliver accurate, sensitive, and precise digital PCR data on QuantStudio 3D Digital PCR instruments when combined with associated reagents and QuantStudio™ 3D Analysis Suite™ software.