Shop All qRT-PCR Kits

CellsDirect™ One-Step qRT-PCR Kit (Invitrogen™)

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features of the CellsDirect™ One-Step qRT-PCR Kit include:

Efficiency—offers significant time and cost savings for increased throughput
Convenience—enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
Sensitivity—no separate RNA isolation step increases sensitivity by minimizing sample loss
Broad detection range—detect targets from samples ranging from a single cell to 10,000 cells
Versatility—compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline your workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for your qRT-PCR application
CellsDirect™ technology is validated for gene expression studies involving limited samples. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (see figure). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan® or LUX™ fluorogenic primers).

Note: This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit (11754-100) with ROX™ Reference Dye included in the 2X Reaction Mix is also available.

Path-ID™ Multiplex One-Step RT-PCR Kit (Applied Biosystems™)

The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR). It is optimized for the amplification of up to four targets simultaneously using your RNA samples and TaqMan® primer-probe sets. Reactions are run using a single-tube, one-step procedure to reverse-transcribe the RNA and amplify your targets. The kit contains a Multiplex Enzyme Mix with Arrayscript™ reverse transcriptase and AmpliTaq Gold® DNA Polymerase, and a buffer mix.

EXPRESS One-Step SYBR™ GreenER™ Kit, with premixed ROX (Invitrogen™)

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This kit comes with premixed ROX reference dye. EXPRESS One-Step SYBR GreenER SuperMix kits with a separate tube of ROX reference dye are also available.

This kit provides:
• Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
• The One-Step SuperScript™ III Reverse Transcriptase for one-step qRT-PCR
• SYBR GreenER dye
• UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

VetMAX™ PRRSV EU & NA 2.0 Kit (Applied Biosystems™)

The VetMAX PRRSV EU & NA 2.0 Kit is a ready-to-use, single-well real-time RT-PCR kit that enables reliable, rapid, and accurate detection of porcine reproductive and respiratory syndrome virus (PRRSV) strains in porcine samples. The European (EU) and North American (NA) strains are detected and differentiated using fluorescent hydrolysis probe chemistry. An exogenous internal positive control (IPC) is included.

The advanced technology of the VetMAX PRRSV EU & NA 2.0 Kit offers several benefits:
Highly accurate―updated design detects the four subtypes of the EU PRRSV Type 1 and NA PRRSV strains, including the HP-PRRSV strain
Fast―results obtained in less than two hours
Flexible―fast and standard amplification modes
Versatile―compatible with a variety of sample matrices, including serum, whole blood, semen, tissue, oral fluid, and cell culture supernatant samples in pools of up to five samples (serum, whole blood, and semen)

The kit offers the convenience of a simple and fast workflow combining the MagMAX CORE nucleic acid purification method with a fast PCR amplification mode for a result in less than two hours.

More than 780 field samples from 13 origins identified as positive or negative for PRRS virus by partner laboratories have been tested. The results showed 99.5% sensitivity and 99.6% specificity.

Verso 1-step RT-qPCR Kit, with separate ROX vial (Thermo Scientific™)

The Thermo Scientific Verso 1-step RT-qPCR Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. This version of the kit comes with a separate vial of ROX reference dye.

Features of the Verso 1-step RT-qPCR Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

Related Products
Verso 1-step RT-qPCR Mix, low ROX
Verso 1-step RT-qPCR Mix, ROX
Verso 1-step RT-qPCR Kit, with separate ROX vial

SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX (Invitrogen™)

Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format. One-Step qRT-PCR kits with ROX include:


–SuperScript® III Reverse Transcriptase: produces higher yields of full-length cDNA for broader gene representation

–Platinum® Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)
–ROX reference dye premixed at optimal concentration for ABI instruments



Two kit configurations allow your choice of detection methods:

–SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit provides a highly efficient, cost-effective qRT-PCR system with SYBR Green I detection
–SuperScript® III Platinum® One-Step qRT-PCR Kit provides sensitive, specific detection for use with LUX™ fluorogenic primers or dual-labeled probes (e.g. TaqMan®) (Figure 2)


Contents and Storage:
SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit with ROX includes SuperScript® III RT/Platinum® Taq Mix, 2X Reaction Mix (containing SYBR® Green I, ROX Reference Dye, dNTPs, and MgSO4), and 50 mM MgSO4. SuperScript® III Platinum® One-Step qRT-PCR Kit with ROX includes SuperScript® III RT/Platinum® Taq Mix, 2X Reaction Mix (containing ROX Reference Dye, dNTPs and MgSO4), and 50 mM MgSO4. Store all components at -20°C. Store ROX Reference dye in the dark. Guaranteed stable for 6 months when properly stored.

EXPRESS One-Step SYBR™ GreenER™ Kit, universal (Invitrogen™)

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This kit comes with a separate tube of ROX reference dye. EXPRESS One-Step SYBR GreenER SuperMix kits with premixed ROX reference dye are also available.

This kit provides:
• Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
• The One-Step SuperScript™ III Reverse Transcriptase for one-step qRT-PCR
• SYBR GreenER dye
• UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

AgPath-ID™ One-Step RT-PCR Reagents with manual (Applied Biosystems™)

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan® real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold® polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization. A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.

TaqPath™ 1-Step Multiplex Master Mix (No ROX) (Applied Biosystems™)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics, virus detection, and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

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This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample (Figure 1). With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix (No ROX) is a superior choice for your multiplex diagnostic testing or development needs.

Features of the TaqPath 1-Step Multiplex Master Mix include:
• High sensitivity to detect low-copy targets with reproducible CT results
• Six logs of dynamic range with both RNA and DNA targets
• Optimized for multiplexing up to four targets simultaneously without a passive reference dye
• Tolerant of inhibitors commonly found in clinical samples

High sensitivity
To enable reproducible detection of low-titer pathogen and transcripts, TaqPath 1-Step Multiplex Master Mix (No ROX) has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 1 shows the consistency of results obtained when detecting 10 copy inputs of RNA target.

Wide dynamic range compatible with RNA and DNA
TaqPath 1-Step Multiplex Master Mix (No ROX) has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath 1-Step Multiplex Master Mix (No ROX) is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 2 demonstrates the excellent PCR linearity across a 6-log RNA dilution series, while Figure 3 exhibits a 6-log linear dynamic range for both RNA and DNA targets.

Optimized for multiplexing
TaqPath 1-Step Multiplex Master Mix has been validated for multiplexing up to four targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes. The mix does not include a passive reference dye, enabling the measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye. Figure 3 demonstrates TaqPath 1-Step Multiplex Master Mix (No ROX) performance in 4-plex reactions with both RNA and cDNA targets.

Inhibitor tolerant
Unlike other master mixes on the market, the unique proprietary formulation of TaqPath 1-Step RT-qPCR Master Mix (No ROX) allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 4).

Manufacturing production and process controls
TaqPath 1-Step Multiplex Master Mix (No ROX) is manufactured under a strong ISO13485 quality management system that uses traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot. Figure 5 demonstrates CT lot-to-lot consistency is preserved across multiple assays with different attributes and input target levels.

Broad instrument compatibility
The TaqPath 1-Step Multiplex Master Mix (No ROX) formulation can be run in either fast or standard cycling conditions with equivalent performance across a wide variety of real-time PCR systems including the Applied Biosystems 7500, 7500 Fast, 7500 Fast Dx, QuantStudio 3, 5, 6, 7, 12k, Dx, and ViiA 7 real-time PCR systems.

General purpose reagent
TaqPath 1-Step RT-qPCR Master Mix is a General Purpose Reagent (GPR), labeled for laboratory use.

Related products
TaqPath 1-Step Master Mix is available in three different formulations:
TaqPath 1-Step qRT-PCR Master Mix—includes ROX dye as the passive reference
TaqPath 1-Step Multiplex Master Mix—includes MUSTANG PURPLE dye as the passive reference
TaqPath 1-Step Multiplex Master Mix (no ROX) (this page)—does not include a passive reference dye

Verso 1-step RT-qPCR Mix, ROX (Thermo Scientific™)

The Thermo Scientific Verso 1-step RT-qPCR ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Features of the Verso 1-step RT-qPCR ROX Mix Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction set up

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

Related Products
Verso 1-step RT-qPCR Mix, low ROX
Verso 1-step RT-qPCR Kit, with separate ROX vial

SuperScript™ III Platinum™ One-Step qRT-PCR Kit (Invitrogen™)

The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.

Features of the SuperScript III Platinum One-Step qRT-PCR Kit include:
• SuperScript III RT for higher temperature cDNA synthesis when addressing difficult RNA secondary structure
• Platinum Taq DNA Polymerase with hot-start technology for improved specificity
• LUX fluorogenic primers and dual-labeled fluorogenic probes for effective detection performance
• Validated performance on multiple real-time instrument platforms, including rotor-based systems

SuperScript III Platinum One-Step qRT-PCR Kits come in multiple configurations:
SuperScript III Platinum One-Step qRT-PCR Kit (this page)
SuperScript III Platinum One-Step qRT-PCR Kit with ROX passive reference
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit with ROX passive reference

SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX (Invitrogen™)

The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.

Features of the SuperScript III Platinum One-Step qRT-PCR Kit include:
• SuperScript III RT for higher temperature cDNA synthesis when addressing difficult RNA secondary structure
• Platinum Taq DNA Polymerase with hot-start technology for improved specificity
• LUX fluorogenic primers and dual-labeled fluorogenic probes for effective detection performance
• ROX reference dye pre-mixed at optimal concentration for Applied Biosystems instruments
• Validated performance on multiple real-time instrument platforms, including rotor-based systems

SuperScript III Platinum One-Step qRT-PCR Kits come in multiple configurations:
SuperScript III Platinum One-Step qRT-PCR Kit with ROX passive reference (this page)
SuperScript III Platinum One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit
SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit with ROX passive reference

TaqMan™ Gene Expression Cells-to-CT™ Kit (Invitrogen™)

The TaqMan® Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification. This kit saves time and offers a simple workflow that is suitable for a few samples or can be easily incorporated into automated, high-throughput applications.

The TaqMan® Gene Expression Cells-to-CT Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, buffer, and TaqMan® Gene Expression Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 2000 real-time PCR results from 400 starting samples

Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® Gene Expression Cells-to-CT™ Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan® Gene Expression Master Mix for real-time PCR analysis. TaqMan® primer⁄probe sets are sold separately.

Simple 7-minute sample preparation: part of a complete workflow
Whether you are using plates or tubes, the TaqMan® Gene Expression Cells-to-CT™ Kit, which uses the simple 7-minute sample preparation procedure outlined (see figure), is designed for 10–100,000 cultured cells⁄sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at -20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

The TaqMan® Gene Expression Cells-to-CT™ Kit workflow enables unsurpassed gene expression evaluation with any of the >700,000 TaqMan® Gene Expression assays. This kit has been extensively tested for specificity with a broad selection of TaqMan® Gene Expression assays and shows performance equivalent to that obtained with purified RNA (see figure).

Achieve unsurpassed performance and sensitivity
Unlike some competitor kits that limit the amount of lysate in the RT reaction to 5%, the TaqMan® Gene Expression Cells-to-CT™ Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan® Gene Expression Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

The ability to detect limited target quantities was tested by mixing a constant number of human cells with various numbers of mouse cells. The cell mixtures were prepared using the TaqMan® Gene Expression Cells-to-CT™ Kit and assayed for a mouse-specific and human-specific gene. Comparative data were generated in the same manner with RNA purified by traditional methodology. The data show that RNA from as few as 10 mouse cells was detectable in a background of RNA from 10,000 human cells (see figure). The ability of the TaqMan® Gene Expression Cells-to-CT™ Kit to detect relative low abundance transcripts was equivalent to that of purified RNA; at low levels, it was superior.

Additionally, the performance of the TaqMan® Gene Expression Cells-to-CT™ Kit was compared to competitor lysate kits and to purified RNA. Inputs of 100–100,000 cells⁄lysis reaction were examined. The sensitivity of the TaqMan® Cells-to-CT™ Kit protocol was equivalent to that obtained with purified RNA, and it surpassed competitor lysates (see figure). Furthermore, the lack of inhibition at high cellular inputs and the low variation among technical replicates demonstrate the reliability of this approach for gene expression studies using cultured cells.

Proven performance, proven together
All components of the TaqMan® Gene Expression Cells-to-CT™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits. And the TaqMan® Gene Expression Cells-to-CT™ Kit has been validated with TaqMan® Gene Expression assays for added quality assurance.

Accessory product
The TaqMan® Cells-to-CT™ Control Kit was designed for use with the TaqMan® Gene Expression Cells-to-CT™ Kit. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding TaqMan® Gene Expression assay to monitor the effects of PCR inhibition.

Verso 1-step RT-qPCR Kit, SYBR Green, low ROX (Thermo Scientific™)

The Thermo Scientific Verso 1-step RT-qPCR Low ROX Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Features of the Verso 1-step RT-qPCR SYBR Green Low ROX Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction setup

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.

Verso 1-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

Related Products
Verso 1-step RT-qPCR Kit, SYBR Green, low ROX
Verso 1-step RT-qPCR Kit, SYBR Green, ROX
Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial

TaqMan™ RNA-to-CT1-Step Kit (Applied Biosystems™)

The Applied Biosystems TaqMan RNA-to-CT 1-Step Kit delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems comprehensive library of TaqMan Gene Expression assays. The kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.

Features of the TaqMan RNA-to-CT 1-Step Kit include:
• Accurate across a wide dynamic range for dependable performance
• Validated with TaqMan Gene Expression Assays for easy experimental setup
• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
• 1-Step format means fewer pipetting steps leading to less chances for errors

Efficiency across a broad range of input concentrations
Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities. Figure 1 shows the excellent amplification efficiency across six orders of magnitude. The result is comparable to that of the TaqMan RNA-to-CT 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan One-Step RT-PCR Master Mix Reagents.

High sensitivity at low target concentration
The sensitivity of TaqMan RNA-to-CT 1-Step Kit was validated using a synthetic RNA template for which copy number is precisely known. Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates. Figure 2 shows the expected quantity of target and corresponding mean CT values. Statistical analysis indicates high confidence of sample quantification based on a T-test (Table I), consistent with as few as 10 copies of target*.

*Results are dependent on a variety of factors, including assay design.