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Luminaris Color Probe qPCR Master Mix, high ROX (Thermo Scientific™)

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG (Applied Biosystems™)

TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-µL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

Convenience
Everything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility
TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

Platinum™ SYBR™ Green qPCR SuperMix-UDG (Invitrogen™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Platinum SYBR Green qPCR SuperMix-UDG and added additional capabilities for your gene expression analysis.

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Platinum® SYBR® Green qPCR SuperMix-UDG provides a sensitive and convenient system for real-time PCR detection with SYBR® Green I dye. The SuperMix format combines amplification and detection reagents into a ready-to-go mix to make set-up easy and ensure reproducible results. Platinum® SYBR® Green qPCR SuperMix-UDG offers:

–Platinum® Taq DNA Polymerase for sensitive amplification from as few as 10 copies of target (Figure 1)

–100% dUTP and UDG enzyme for superior control of carryover contamination, saving time by reducing the number of failed experiments
–Separate tubes of ROX reference dye and BSA for easy optimization on 96-well plates and glass capillary tube instruments (Figure 2)
–SYBR® Green I dye for simple and easy detection of real-time product

DyNAmo Flash SYBR Green qPCR Kit (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo Flash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo Flash SYBR Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantification and early Cq values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.

Highlights

• Extremely fast protocols: Combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2× master mix and optimized protocol

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNAmo Flash Probe qPCR Kit
DyNAmo Flash SYBR Green qPCR Kit
DyNAmo HS SYBR Green qPCR Kit

Power SYBR™ Green PCR Master Mix (Applied Biosystems™)

Get superior nucleic acid quantitation with Applied Biosystems® Power SYBR® Green Master Mix. Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

• Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR® Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR® Green reagent-based real-time PCR
• The optimized formulation contains highly purified AmpliTaq Gold® DNA Polymerase, LD to offer greater sensitivity than classic SYBR® Green PCR master mix
Power SYBR® Green PCR Master Mix contains a blend of dTTPÚdUTP that is compatible with AmpErase® UNG, to minimize carryover contamination
• Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations to minimize well-to-well variability that result from a variety of causes, such as pipetting error and sample evaporation
Power SYBR® Green reagent-based PCR chemistry easily replaces the existing SYBR® Green PCR Master Mix in Applied Biosystems® protocols using the same setup and thermal cycling conditions

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

Ion Universal Library Quantitation Kit

The Ion Universal Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input generated by the Ion 16STM Metagenomics Kit for Ion Torrent™ semiconductor sequencing. This kit provides the TaqMan® assay, TaqMan® Universal Master Mix, and library standard needed to quantify femtomolar amounts of Ion fragment libraries. With cycling conditions that allow both fast and standard cycling, the Ion Universal Library Quantitation Kit supports most real-time platforms. This real-time PCR kit enables greater accuracy and specificity compared to non-PCR or non-probe-based methods, delivering simplicity and speed to the Ion PGM™ System sequencing workflow.

The Ion Universal Library Quantitation Kit enables:
• Precision—TaqMan® assays enable greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods
• Sensitivity—the protocol supports quantitation of femtomolar amounts of fragment libraries
• Simplicity—the validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation
• Versatility—the highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range
• Efficiency—qPCR-based methods use less material for quantification

This kit includes:
• A TaqMan® assay designed for Ion library adaptors
• TaqMan® Universal PCR Master Mix
• An adapted E. coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ~190 bp in size

Note: In addition to use with the Ion 16S™ Metagenomics Kit workflow, the Ion Universal Library Quantitation kit is also compatible with sequencing workflows for amplicons generated using any master mix.

Luminaris Color HiGreen qPCR Master Mix, fluorescein (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

TaqPath™ ProAmp™ Multiplex Master Mix (Applied Biosystems™)

TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Multiplex Master Mix is manufactured under a strong ISO13485 quality management system and designed to produce consistent product performance lot after lot.

Request an evaluation sample ›

Two TaqPath ProAmp Master Mix formulations for different throughput needs
TaqPath ProAmp Master Mix—includes ROX passive reference dye for lower multiplexing of up to three targets
• TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE passive reference dye for higher multiplexing of up to four targets

Benefits of TaqPath ProAmp Master Mix
Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock Taq DNA hot-start mechanism
Multiplexing breadth—two formulations that enable single to four-target detection per reaction
Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mix has been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. The figure below shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan genotyping assays using crude lysate from blood samples. TaqPath ProAmp Master Mix has been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High accuracy copy number quantitation
The versatile TaqPath ProAmp Master Mix can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. The figure below shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp Master Mix enables determination of copy number accurately in samples with up to four copies.

General purpose reagents
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled "For Laboratory Use."

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›

* Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.

SYBR™ Green PCR Master Mix

The SYBR® Green PCR Master Mix, optimized for real-time PCR analysis, conveniently combines SYBR Green 1 Dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer components in easy-to-use premix vials.


• Easy-to-use solution contains all components needed for PCR applications using SYBR® Green dye, which minimizes assay set-up time
• Used with rapid assay development guidelines, that minimize optimization time
• SYBR® Green I dye detects double-stranded DNA, so specific probes are not required
• Includes Passive Reference I, which is required for signal normalization in all SYBR® Green dye reactions
• Contains AmpliTaq Gold® DNA polymerase, which allows the highest performance available by minimizing non-specific product formation (including primer-dimer)
• Optimized buffer components are proprietary buffer enhancements that add to the performance and reliability of the assay using SYBR® Green dye
• Recommended storage conditions are 2°-8°C, so no thawing is required before use, thereby minimizing assay set-up time


Applied Biosystems has developed a comprehensive set of guidelines to ensure success when using sequence detection systems (SDS) reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

All SDS reagent kits contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that result from a variety of causes, such as pipetting errors and sample evaporation.

Real-time quantitative PCR was first performed using the 5' fluorogenic nuclease assay, which uses a TaqMan® probe during the PCR amplification to provide added specificity. An additional chemistry is also available, using SYBR® Green I dye, that can provide real-time quantitative PCR information. Assays using the SYBR® Green 1 double-stranded DNA binding dye do not require a TaqMan® probe and provide additional experimental flexibility.

The incorporation of SYBR Green I dye into a real-time PCR reaction lets the user detect any double-stranded DNA generated during PCR. This provides great flexibility because no target specific probes are required, and yet both specific and non-specific products will generate signal. The use of the hot-start enzyme AmpliTaq Gold® DNA Polymerase in all SYBR Green I reagent kits yields the highest performance available by minimizing non-specific product formation. SYBR Green I dyes are ideal for use in target identification (screening assays), or when only a small number of reactions are required for a given assay.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

SYBR GreenER™ qPCR SuperMix for iCycler™ Instrument (Invitrogen™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR GreenER qPCR SuperMix and added additional capabilities for your gene expression analysis.

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SYBR® GreenER™ qPCR SuperMixes for iCycler® incorporate the latest high-performance technology to provide the most reliable gene expression data from your iCycler iQ® or MyiQ® instrument. The SYBR® GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR® Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR® GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR® GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR® GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR® GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

For Research Use Only. Not for use in diagnostics procedures.

Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green qPCR Master Mix, ROX Solution provided (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Path-ID™ qPCR Master Mix (Applied Biosystems™)

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.

ABsolute QPCR Mix, SYBR Green, ROX (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

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Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

Highlights

• High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

TaqMan™ PreAmp Master Mix (Applied Biosystems™)

TaqMan® PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan® PreAmp Master Mix lets you:

• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan® Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Preamplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan® PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Easy Workflow
With TaqMan® PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan® assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan® PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp® 9700 thermal cycler.

Luminaris HiGreen qPCR Master Mix, low ROX (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.