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TaqMan™ Universal Master Mix II, no UNG (Applied Biosystems™)

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Optimized buffer components

Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping Assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

Path-ID™ qPCR Master Mix with Manual (Applied Biosystems™)

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.

Path-ID™ qPCR Master Mix (Applied Biosystems™)

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.

DyNAmo HS SYBR Green qPCR Kit (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo HS SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo HS SYBR Green qPCR Kit contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.

ROX passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.

Highlights

• Strong specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup
• Highly sensitive detection: Optimized master mix allows reproducible detection of low-copy number templates
• Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude
• Convenient to use: 2X master mix, one tube only. ROX passive reference dye provided in a separate vial

Related Products
DyNAmo Flash Probe qPCR Kit
DyNAmo Flash SYBR Green qPCR Kit
DyNAmo HS SYBR Green qPCR Kit

SYBR GreenER™ qPCR SuperMix for ABI PRISM™ Instrument (Invitrogen™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR GreenER qPCR SuperMix and added additional capabilities for your gene expression analysis.

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SYBR® GreenER™ qPCR SuperMixes for ABI PRISM® incorporate the latest high-performance technology to provide the most reliable gene expression data from your ABI 7300, 7000, 7700 or 7900HT instrument. The SYBR® GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR® Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR® GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR® GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR® GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR® GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

For Research Use Only. Not for use in diagnostics procedures.

Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/Fluorescein qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with fluorescein passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Luminaris Color HiGreen qPCR Master Mix, fluorescein (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

TaqMan™ Universal PCR Master Mix (Applied Biosystems™)

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• AmpErase UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination
• Rapid assay development guidelines are provided to minimize optimization time

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

DyNAmo Flash SYBR Green qPCR Kit (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo Flash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo Flash SYBR Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantification and early Cq values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.

Highlights

• Extremely fast protocols: Combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2× master mix and optimized protocol

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNAmo Flash Probe qPCR Kit
DyNAmo Flash SYBR Green qPCR Kit
DyNAmo HS SYBR Green qPCR Kit

TaqMan™ PreAmp Master Mix (Applied Biosystems™)

TaqMan® PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan® PreAmp Master Mix lets you:

• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE)
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan® Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Preamplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan® PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Easy Workflow
With TaqMan® PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan® assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan® PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp® 9700 thermal cycler.

Luminaris Color HiGreen qPCR Master Mix (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.

PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™)

PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range
PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

UDG included for carryover contamination control
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

Stability of pre-assembled reactions
The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios1.

Broad instrument compatibility
PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.

Learn more about PowerUp™ SYBR® Green Master Mix >

Request a free sample >

1 Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.
2 For optimal performance, standard cycling using the Applied Biosystems® 7900 system is recommended.

TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG (Applied Biosystems™)

TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-µL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

Convenience
Everything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility
TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

Luminaris Color HiGreen qPCR Master Mix, low ROX (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

VetMAX™ M. tuberculosis Complex PCR Kit (Applied Biosystems™)

The VetMAX M. tuberculosis Complex PCR Kit is a single-well, duplex, real-time PCR assay for the detection of all mycobacteria belonging to the Mycobacterium tuberculosis Complex in bovine lymph nodes and surrounding tissues. The kit is a highly sensitive and specific test for the detection of bovine tuberculosis, allowing reliable and rapid screening, exclusion of non-infected animals, and confirmation of infected animals.

All components needed for the PCR reaction are included in the kit:
• Mix MTBC: contains TaqMan primers and probes, buffer, and enzyme for optimized duplex real-time PCR amplification of targets
• EPC MTBC: DNA template containing the M. tuberculosis Complex target sequence (external positive control). It serves as a positive control for the real-time PCR components and is used to set the threshold cycle (Ct) for evaluating test results
• IPC MTBC: added to each test and control sample at the lysis step of the DNA isolation procedure (exogenous internal positive control). The IPC serves as a control for the DNA isolation procedure and is used to monitor for the presence of PCR inhibitors

The VetMAX M. tuberculosis Complex PCR Kit is part of a workflow solution with the MagVet Universal Isolation Kit (Cat. No. MV384) and the Kingfisher Flex Magnetic Particle Processor or Kingfisher mL Flex Magnetic Particle Processor (Cat. Nos. 5400630 and 5400640).