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    TaqMan™ Universal Master Mix II, no UNG Applied Biosystems™

    TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

    This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
    • AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
    • dNTPs (with dUTP)
    • ROX Passive Reference
    • Optimized buffer components

    Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

    TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
    • TaqMan gene expression assays
    • TaqMan microRNA assays
    • TaqMan drug metabolism genotyping assays
    • TaqMan SNP genotyping Assays

    For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

    Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

    EXPRESS qPCR Supermix, with premixed ROX Invitrogen™

    Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of EXPRESS qPCR SuperMix and added additional capabilities for your gene expression analysis.

    EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays. This kit comes with premixed ROX reference dye. EXPRESS qPCR SuperMix kits with a separate tube of ROX reference dye are also available.

    This kit provides:
    • Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
    • UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

    TaqMan™ Universal Master Mix II, with UNG Applied Biosystems™

    TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

    This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
    • AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
    • dNTPs (with dUTP)
    • ROX Passive Reference
    • Uracil-N-glycosylase (UNG)
    • Optimized buffer components

    Note: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

    TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
    • TaqMan gene expression assays
    • TaqMan microRNA assays
    • TaqMan drug metabolism genotyping assays
    • TaqMan SNP genotyping assays

    For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

    Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

    QuantStudio™ 12K Flex OpenArray™ Gene Expression Starter Kit Applied Biosystems™

    A variety of starter kits are available to introduce you to the various applications of the QuantStudio™12K Flex OpenArray® sample block. Each starter kit contains 2 arrays, plus sufficient reagents and accessories to perform two experiments. The Gene Expression starter kit contains 2 gene expression endogenous control plates. The Gene Expression kit also contains cDNA controls to serve as samples for your experiments. Note that the QuantStudio™12K Flex Software (included with the QuantStudio™ 12K Flex System) offers example experiment files for each application.

    The accessories provided in the kit include: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.

    PowerUp™ SYBR™ Green Master Mix Applied Biosystems™

    PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

    PowerUp™ SYBR® Green Master Mix features include:
    • A dual hot-start mechanism for excellent specificity
    • Highly reproducible CTs over a broad dynamic range
    • Inclusion of UDG to help prevent carryover contamination
    • Stability of pre-assembled reactions for up to 72 hours
    • Compatibility with most real-time qPCR instruments

    Exceptional specificity with dual hot-start mechanism
    Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

    Tight reproducibility in CTs over a wide dynamic range
    PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

    UDG included for carryover contamination control
    The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

    Stability of pre-assembled reactions
    The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios1.

    Broad instrument compatibility
    PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.

    Learn more about PowerUp™ SYBR® Green Master Mix >

    1 Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.
    2 For optimal performance, standard cycling using the Applied Biosystems® 7900 system is recommended.

    ABsolute QPCR Mix, SYBR Green, fluorescein Thermo Scientific™

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    Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

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    Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

    ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

    Highlights

    • High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
    • Optimised buffer containing SYBR Green I
    • Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
    • Broad dynamic range

    Applications

    • Gene expression analysis
    • siRNA validation
    • Genotyping

    Related Products
    ABsolute QPCR Mix, no ROX
    ABsolute QPCR Mix, low ROX
    ABsolute QPCR Mix, ROX
    ABsolute QPCR Capillary Mix
    ABsolute QPCR Mix, SYBR Green, no ROX
    ABsolute QPCR Mix, SYBR Green, low ROX
    ABsolute QPCR Mix, SYBR Green, ROX
    ABsolute QPCR Mix, SYBR Green, fluorescein
    ABsolute QPCR Capillary Mix, SYBR Green

    Luminaris Probe qPCR Master Mix, high ROX Thermo Scientific™

    Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

    The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

    The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

    Highlights

    • UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
    • Sensitivitity—single copy detection
    • Wide linear range—accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience— ready-to-use 2X master mix
    • Equal performance of blue and colorless master mixes
    • Versions with premixed ROX for different real-time platforms
    • Standard cycling protocols

    Applications

    • Gene expression analysis
    • siRNA validation
    • Genotyping
    • Pathogen detection

    Includes

    Luminaris Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

    Luminaris Probe qPCR Master Mix, low ROX Thermo Scientific™

    Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

    The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

    The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

    Highlights

    • UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
    • Sensitivitity—single copy detection
    • Wide linear range—accurate quantification across 9 orders of magnitude
    • Reproducibility and convenience— ready-to-use 2X master mix
    • Equal performance of blue and colorless master mixes
    • Versions with premixed ROX for different real-time platforms
    • Standard cycling protocols

    Applications

    • Gene expression analysis
    • siRNA validation
    • Genotyping
    • Pathogen detection

    Includes

    Luminaris Probe Low ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

    Maxima SYBR Green/ROX qPCR Master Mix (2X) Thermo Scientific™

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    Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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    Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

    Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

    Highlights

    SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
    Sensitivity—detects low copy number targets
    Wide linear range—accurate quantification across 9 orders of magnitude
    Reproducibility and convenience—ready-to-use 2x master mix

    Applications

    • Gene expression
    • siRNA validation
    • Genotyping
    • Pathogen detection

    Includes

    Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

    Related Products
    Maxima SYBR Green/ROX qPCR Master Mix (2X)
    Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
    Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

    ABsolute Blue QPCR Mix, SYBR Green, fluorescein Thermo Scientific™

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    Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

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    Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

    Highlights

    High performance—ABsolute sensitivity, consistency & convenience
    Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
    Universal—compatible with all major qPCR platforms
    Adaptable—available with ROX or Fluorescein passive reference dyes

    Applications

    • Gene expression analysis
    • siRNA validation
    • Genotyping

    Includes
    • ABsolute Blue qPCR SYBR Green Fluorescein Mix contains 2X master mix supplemented with fluorescein

    Related Products
    ABsolute Blue QPCR Mix, low ROX
    ABsolute Blue QPCR Mix, with separate ROX vial
    ABsolute Blue QPCR Mix, ROX
    ABsolute Blue QPCR Mix, SYBR Green, low ROX
    ABsolute Blue QPCR Mix, SYBR Green, ROX
    ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
    ABsolute Blue QPCR Mix, SYBR Green, fluorescein

    ABsolute QPCR Mix, low ROX Thermo Scientific™

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    Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute QPCR Mix and added additional capabilities for your gene expression analysis.

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    Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

    Highlights

    • High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
    • Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
    • Optimised buffer - For maximal performance in multiplex reactions

    Applications
    • Gene expression analysis
    • siRNA validation
    • Genotyping

    Related Products
    ABsolute QPCR Mix, no ROX
    ABsolute QPCR Mix, low ROX
    ABsolute QPCR Mix, ROX
    ABsolute QPCR Capillary Mix
    ABsolute QPCR Mix, SYBR Green, no ROX
    ABsolute QPCR Mix, SYBR Green, low ROX
    ABsolute QPCR Mix, SYBR Green, ROX
    ABsolute QPCR Mix, SYBR Green, fluorescein
    ABsolute QPCR Capillary Mix, SYBR Green

    ABsolute QPCR Capillary Mix Thermo Scientific™

    Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.


    Highlights
    • High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
    • Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
    • Optimised buffer - For maximal performance in multiplex reactions


    Applications
    • Gene expression analysis
    • siRNA validation
    • Genotyping


    Includes
    • Absolute qPCR Mix (no ROX) contains 2X Master Mix and no passive reference dye
    • Absolute qPCR ROX (500 nm) Mix contains 2X Master Mix supplemented with high ROX concentration
    • Absolute qPCR Low ROX Mix contains 2X Master Mix supplemented with low ROX concentration
    • Absolute qPCR Capillary Mix contains 2X Master Mix optimized for capillary instruments

    VetMAX™ African Swine Fever Virus Detection Kit

    The VetMAX African Swine Fever Virus Detection Kit is a single-well duplex real-time PCR for the detection of African Swine Fever Virus (ASFV) DNA in serum, whole blood, and tissue samples of pigs using TaqMan primer and probes.

    This kit enables detection in individual samples or pools of up to 10 samples.

    The kit is sensitive and specific for the detection of ASFV DNA, allowing for reliable and rapid screening during outbreaks and monitoring situations. It has been validated by the European Union Reference Laboratory.The kit has been used since 2015 during outbreaks in Eastern Europe for clinical sign confirmation and detection of domestic and wild pigs affected by ASFV.

    The kit includes:
    • A ready-to-use master mix for the detection of the ASFV target and the internal positive control
    • A positive control for ASFV
    • An internal positive control

    TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG Applied Biosystems™

    TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-µL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

    Convenience
    Everything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

    Compatibility
    TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.

    Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

    Platinum™ SYBR™ Green qPCR SuperMix-UDG w/ROX Invitrogen™

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    Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Platinum SYBR Green qPCR SuperMix-UDG and added additional capabilities for your gene expression analysis.

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    qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:


    –Platinum® Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)

    –Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
    –ROX reference dye premixed at optimal concentration for ABI instruments


    qPCR SuperMixes with ROX are available in two formats:

    –Platinum® SYBR® Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR® Green I dye (Figure 2)
    –Platinum® qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan®)


    Contents and Storage:
    Platinum® SYBR® Green qPCR SuperMix-UDG with ROX includes a 2X SuperMix with SYBR® Green I and ROX Reference Dyes, and 50 mM MgCl2. Platinum® qPCR SuperMix-UDG with ROX includes a 2X SuperMix with ROX Reference Dye, and 50 mM MgCl2. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.
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