Shop All qPCR Kits

TaqMan™ OpenArray™ Real-Time PCR Master Mix (Applied Biosystems™)

TaqMan® OpenArray® Real-Time PCR Master Mix is optimized for use with TaqMan® OpenArray® gene expression panels, and yields reproducible, specific, and sensitive results. This reagent is suited for OpenArray® MicroRNA panels and other OpenArray® gene expression applications.

Key Product Features:

• Hot start chemistry to provide room temperature stability
• Optimized for use with TaqMan® OpenArray® gene expression panels yielding reproducible, specific, and sensitive results
• For use with OpenArray® MicroRNA panels, OpenArray® gene expression pathway panels, and other gene expression applications on the OpenArray® format
• Ready for use with no additional additives required

For more detailed performance data, refer to the TaqMan® OpenArray® MicroRNA Panels product information.

As an integral component of the TaqMan® OpenArray® miRNA profiling application, TaqMan® OpenArray® Real-Time PCR Master Mix delivers high sensitivity and reproducibility with the convenience of a fully formulated master mix.

PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™)

For qPCR users using SYBR green dye who need to minimize optimization time, Applied Biosystems PowerUp SYBR Green Master Mix offers proprietary Dual-Lock hot start technology. Unlike other polymerases, a Dual-Lock polymerase offers two unique hot start mechanisms to prevent primer dimer and non-specific amplification.

PowerUp SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp SYBR Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp SYBR Green Master Mix uses the Dual-Lock Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp SYBR Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range
PowerUp SYBR Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp SYBR Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

UDG included for carryover contamination control1
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp SYBR Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

Stability of pre-assembled reactions
The tight control of the Dual-Lock Taq DNA polymerase in PowerUp SYBR Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP SYBR Green Master Mix across numerous workflow scenarios.2

Broad instrument compatibility
PowerUp SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments.3 It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems. For optimal results, recommended primer concentrations are 300–800 nM.

Learn more about PowerUp SYBR Green Master Mix >

Request a free sample >

1 Master mixes containing UDG should not be used with bisulfite-treated DNA samples.
2 Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.
3 For optimal performance, standard cycling using the Applied Biosystems 7900 system is recommended.

Luminaris Color HiGreen qPCR Master Mix, low ROX (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Excellent qPCR performance:
Specificity—Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—single copy detection
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

VetMAX™ MastiType Multi Kit (Applied Biosystems™)

The VetMAX MastiType Multi Kit enables rapid, accurate detection of 15 mastitis-causing pathogens and one penicillin-resistance gene in bovine milk. These 16 markers are detected using real-time PCR amplification of DNA unique to each pathogen in four separate reactions.

The advanced technology of the VetMAX MastiType real-time PCR assays offers these advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation

The VetMAX MastiType Multi assay detects these 15 pathogens and one resistance gene in four separate PCR reactions:
Staphylococcus aureus
Staphylococcus spp. (including all major coagulase-negative staphylococci)
Streptococcus agalactiae
Streptococcus dysgalactiae
Streptococcus uberis
Escherichia coli
Enterococcus spp. (including E. faecalis and E. faecium)
Klebsiella oxytoca (and/or K. pneumoniae)
Serratia marcescens
Corynebacterium bovis
Trueperella pyogenes and/or Peptoniphilus indolicus
• Staphylococcal β-lactamase gene (penicillin-resistance gene)
Mycoplasma bovis
Mycoplasma spp.
• Yeast
Prototheca spp.

SYBR™ Select Master Mix (Applied Biosystems™)

SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR® Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR Select Master Mix is specially formulated to provide robust results at 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.

View performance data.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.

ABsolute Blue QPCR Mix, SYBR Green, ROX (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Includes
• ABsolute Blue qPCR SYBR Green ROX Mix contains 2X master mix supplemented with high ROX reference dye concentration

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

Platinum™ SYBR™ Green qPCR SuperMix-UDG (Invitrogen™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Platinum SYBR Green qPCR SuperMix-UDG and added additional capabilities for your gene expression analysis.

**********************
Platinum® SYBR® Green qPCR SuperMix-UDG provides a sensitive and convenient system for real-time PCR detection with SYBR® Green I dye. The SuperMix format combines amplification and detection reagents into a ready-to-go mix to make set-up easy and ensure reproducible results. Platinum® SYBR® Green qPCR SuperMix-UDG offers:

–Platinum® Taq DNA Polymerase for sensitive amplification from as few as 10 copies of target (Figure 1)

–100% dUTP and UDG enzyme for superior control of carryover contamination, saving time by reducing the number of failed experiments
–Separate tubes of ROX reference dye and BSA for easy optimization on 96-well plates and glass capillary tube instruments (Figure 2)
–SYBR® Green I dye for simple and easy detection of real-time product

VetMAX™ MastiType Micro4 Kit (Applied Biosystems™)

The VetMAX MastiType Micro4 Kit enables rapid, accurate detection of the four major contagious mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen in a single reaction.

The advanced technology of VetMAX MastiType real-time PCR assays offers several advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation

The VetMAX MastiType Micro4 assay detects these four pathogens in a single PCR reaction:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Streptococcus uberis

SYBR™ Select Master Mix for CFX (Applied Biosystems™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix for CFX and added additional capabilities for your gene expression analysis.

**********************
SYBR® Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity
SYBR® Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye, resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

View performance data.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

TaqMan™ Fast Advanced Master Mix (Applied Biosystems™)

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It provides best in class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

Features of the TaqMan Fast Advanced Master Mix include:
Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
Reduced run times (<40 minutes) on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
Seamlessly transitions into your experiments—validated with TaqMan assays for gene expression and microRNAs, and TaqMan array micro-fluidic cards

Best-in-class performance
TaqMan Fast Advanced Master Mix has been designed to provide performance equal to or better than standard master mixes. It has been benchmarked against the leading suppliers' standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. TaqMan Fast Advanced Master Mix’s dynamic range (up to 7 logs) is the widest in the industry. The impressive sensitivity of the mix is showcased in the figure below which compares the Ct values of TaqMan Fast Advanced Master Mix and TaqMan Universal PCR Master Mix across a panel of gene expression assays.

Benchtop stability for high-throughput handling and convenience
TaqMan Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. The figure below shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.

For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running plates upon assembly.

Optimized for multiplexing
We realize that confidence is paramount when it comes to your results. For added confidence in the results in every well, TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). The figure below shows results for the experimental target β-actin (gene name: ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.

Validated for microRNA assays
TaqMan Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The data in the figure below demonstrate excellent PCR linearity over a 6-log range of input template.

Reduced run times on standard instrumentation
TaqMan Fast Advanced Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides. The figure below showcases the impressive results achieved when using TaqMan Fast Advanced Master Mix under fast thermal cycling conditions on the Applied Biosystems 7300 Real-Time PCR System. The mix has been tested with all Applied Biosystems standard real-time PCR instrumentation (7900HT, 7500, and 7300 systems) to enable success independent of whether or not you own a fast-enabled instrument.

DyNAmo Flash SYBR Green qPCR Kit (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo Flash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific DyNAmo Flash SYBR Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantification and early Cq values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.

Highlights

• Extremely fast protocols: Combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2× master mix and optimized protocol

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNAmo Flash Probe qPCR Kit
DyNAmo Flash SYBR Green qPCR Kit
DyNAmo HS SYBR Green qPCR Kit

Fast SYBR™ Green Master Mix (Applied Biosystems™)

Fast SYBR® Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR® Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR® Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.

Luminaris HiGreen qPCR Master Mix, high ROX (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: ingle copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen High ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

SYBR™ Green PCR Master Mix (Applied Biosystems™)

Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
• Premixed components stored at 2–8°C significantly reduce assay setup time
• SYBR® Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability Maximum Flexibility and Convenience Applied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan® probes are required. SYBR® Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR® Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

Path-ID™ qPCR Master Mix (Applied Biosystems™)

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.

ABsolute QPCR Mix, no ROX (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Highlights

• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

Applications
• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

VetMAX™ MastiType Multi Combo Kit (Applied Biosystems™)

This combo kit includes the VetMAX MastiType Multi Kit and the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module (also available separately).

VetMAX MastiType Multi Kit
The VetMAX MastiType Multi Kit enables rapid, accurate detection of 15 mastitis-causing pathogens and one penicillin-resistance gene in bovine milk. These 16 markers are detected using real-time PCR amplification of DNA unique to each pathogen in four separate reactions. The kit detects these mastitis-causing pathogens:
Staphylococcus aureus
Staphylococcus spp. (including all major coagulase-negative staphylococci)
Streptococcus agalactiae
Streptococcus dysgalactiae
Streptococcus uberis
Escherichia coli
Enterococcus spp. (including E. faecalis and E. faecium)
Klebsiella oxytoca (and/or K. pneumoniae)
Serratia marcescens
Corynebacterium bovis
Trueperella pyogenes and/or Peptoniphilus indolicus
• Staphylococcal β-lactamase gene (penicillin-resistance gene)
Mycoplasma bovis
Mycoplasma spp.
• Yeast
Prototheca spp.

Learn more about the VetMAX MastiType Multi Kit ›

For Veterinary Use Only. For In Vitro Use Only.

MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module
The MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

The MagMAX CORE Mastitis & Panbacteria Module is offered here with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of all bacteria, especially those important in the detection of mastitis in cattle and from challenging matrices like mastitic milk. We have included a specially optimized lysis solution, along with an adjusted extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram-positive bacteria.

Learn more about the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module ›

For Laboratory Use Including Veterinary and Environmental Uses.

VetMAX™ MastiType Myco8 Kit (Applied Biosystems™)

The VetMAX MastiType Myco8 Kit enables rapid, accurate detection of eight mastitis-causing pathogens in bovine milk. These eight pathogens, including six highly contagious and difficult to culture Mycoplasma strains, are detected using real-time PCR amplification of DNA unique to each pathogen in two separate reactions.

The advanced technology of the VetMAX MastiType real-time PCR assays offers these advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation

The VetMAX MastiType Myco8 assay detects these eight pathogens in two separate PCR reactions:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Mycoplasma spp.
Mycoplasma alkalescens
Mycoplasma bovigenitalium
Mycoplasma californicum
Mycoplasma canadense

Luminaris HiGreen qPCR Master Mix (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights:

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix

Applications:

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes:

Luminaris HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer; supplied with nuclease-free water.

DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo ColorFlash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific DyNAmo ColorFlash SYBR Green qPCR Kit offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

• Colored reagents minimize the risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2x master mix allows the use of UNG for prevention of carry-over contamination

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit

Luminaris Probe qPCR Master Mix, low ROX (Thermo Scientific™)

Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Probe Low ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG (Applied Biosystems™)

TaqMan Universal PCR Master Mix, No AmpErase UNG, is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• Contains dUTP in the dNTP mix to protect against carry-over contamination, when used with AmpErase UNG
• Rapid assay development guidelines are provided to minimize optimization time

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

Platinum™ Quantitative PCR SuperMix-UDG (Invitrogen™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.

**********************
Platinum® Quantitative PCR SuperMix-UDG is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2). The system is optimized to provide superior performance with either LUX™ Fluorogenic Primers or probe-based detection systems.

Platinum® Quantitative PCR SuperMix-UDG:

• Is highly sensitive and specific
• Allows room temperature reaction assembly (3)
• Is easy to use-just add template and fluorogenic primers or probes for detection
• Includes a vial of 50 mM MgCl2 to accommodate a variety of primer/template PCR systems
• Is compatible with most real-time instruments

Applications: Real-time quantitative amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, and fluorometric SNP (Single Nucleotide Polymorphism) detection.

Luminaris Color Probe qPCR Master Mix (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.

Maxima SYBR Green/ROX qPCR Master Mix (2X) (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Path-ID™ qPCR Master Mix with Manual (Applied Biosystems™)

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.

SYBR GreenER™ qPCR SuperMix Universal (Invitrogen™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR GreenER qPCR SuperMix and added additional capabilities for your gene expression analysis.

**********************
SYBR® GreenER™ qPCR SuperMixes Universal incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments. The SYBR® GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR® Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR® GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR® GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR® GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR® GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

Eight cDNA targets and eight genomic DNA targets were amplified from 1 ng of cDNA and 6 pg of gDNA, respectively, on the ABI PRISM® 7900HT Sequence Detection System using the SYBR® GreenER™ qPCR SuperMix for ABI PRISM® instruments. For all 16 targets, detection was positive in the presence of template but negative for all no-template controls.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Universal PCR Master Mix (Applied Biosystems™)

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance every time.

• Contains AmpliTaq Gold DNA Polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq DNA Polymerase
• Includes a passive internal reference based on proprietary ROX dye, for superb precision on Applied Biosystems real-time PCR instruments
• Buffer enhancements guarantee performance and reliability in all applications, even with G/C-rich sequences
• AmpErase UNG protects against subsequent re-amplification from PCR products containing dU to minimize carry-over contamination
• Rapid assay development guidelines are provided to minimize optimization time

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

TaqMan™ Gene Expression Master Mix (Applied Biosystems™)

Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan Gene Expression Master Mix has been validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications, including:
• Rare transcript detection
• Gene expression analysis
• Pathogen detection and viral load quantification
• Co-amplification of two targets
• Specific detection of gene family members

TaqMan Gene Expression Master Mix contains AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Gene Expression Master Mix and added additional capabilities for your gene expression analysis.

TaqMan™ PreAmp Master Mix (Applied Biosystems™)

TaqMan® PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan® PreAmp Master Mix lets you:

• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE)
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan® Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Preamplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan® PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Easy Workflow
With TaqMan® PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan® assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan® PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp® 9700 thermal cycler.

EXPRESS qPCR Supermix, with premixed ROX (Invitrogen™)

Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of EXPRESS qPCR SuperMix and added additional capabilities for your gene expression analysis.

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays. This kit comes with premixed ROX reference dye. EXPRESS qPCR SuperMix kits with a separate tube of ROX reference dye are also available.

This kit provides:
• Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
• UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/Fluorescein qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with fluorescein passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

TaqMan™ Universal Master Mix II, with UNG (Applied Biosystems™)

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer components

Note: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

Luminaris Color HiGreen qPCR Master Mix, fluorescein (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green qPCR Master Mix, ROX Solution provided (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

VetMAX™ MastiType Myco8 Combo Kit (Applied Biosystems™)

This combo kit includes the VetMAX MastiType Myco8 qPCR Kit and the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module (also available separately).

VetMAX MastiType Myco8 Kit
The VetMAX MastiType Myco8 assay enables rapid, accurate detection of eight mastitis-causing pathogens in bovine milk. These eight pathogens, including six highly contagious and difficult to culture Mycoplasma strains, are detected using real-time PCR amplification of DNA unique to each pathogen in two separate reactions. The kit detects these eight mastitis-causing pathogens:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Mycoplasma spp.
Mycoplasma alkalescens
Mycoplasma bovigenitalium
Mycoplasma californicum
Mycoplasma canadense

Learn more about the VetMAX MastiType Myco8 Kit >

MagMAX CORE Nucleic Acid Purification Kit
The Applied Biosystems MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

Learn more about the MagMAX CORE Nucleic Acid Purification Kit >

MagMAX CORE Mastitis & Panbacteria Module
The MagMAX CORE Mastitis & Panbacteria Module is offered here in combination with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of all bacteria, especially those important in the detection of mastitis in cattle and from challenging matrices like mastitic milk.

We have included a specially optimized lysis solution, along with an adjusted extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram-positive bacteria.

Features of this combined product include:
Convenience—a complete solution using the same CORE chemistry
Easy—no centrifugation and no aspiration steps
Fast—less than 1 hour for 96 samples
Flexible—appropriate for fresh, frozen, or preserved milk samples, including samples with high somatic cell count

The MagMAX CORE kit and the MagMAX CORE Mastitis & Panbacteria Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.

ABsolute Blue QPCR Mix, SYBR Green, low ROX (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Includes
• ABsolute Blue qPCR SYBR Green low ROX Mix contains 2X master mix supplemented with low ROX reference dye concentration

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

SYBR GreenER™ qPCR SuperMix for ABI PRISM™ Instrument (Invitrogen™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR GreenER qPCR SuperMix and added additional capabilities for your gene expression analysis.

**********************
SYBR® GreenER™ qPCR SuperMixes for ABI PRISM® incorporate the latest high-performance technology to provide the most reliable gene expression data from your ABI 7300, 7000, 7700 or 7900HT instrument. The SYBR® GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR® Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR® GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR® GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR® GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR® GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

For Research Use Only. Not for use in diagnostics procedures.

VetMAX™ M. tuberculosis Complex PCR Kit (Applied Biosystems™)

The VetMAX M. tuberculosis Complex PCR Kit is a single-well, duplex, real-time PCR assay for the detection of all mycobacteria belonging to the Mycobacterium tuberculosis Complex in bovine lymph nodes and surrounding tissues. The kit is a highly sensitive and specific test for the detection of bovine tuberculosis, allowing reliable and rapid screening, exclusion of non-infected animals, and confirmation of infected animals.

All components needed for the PCR reaction are included in the kit:
• Mix MTBC: contains TaqMan primers and probes, buffer, and enzyme for optimized duplex real-time PCR amplification of targets
• EPC MTBC: DNA template containing the M. tuberculosis Complex target sequence (external positive control). It serves as a positive control for the real-time PCR components and is used to set the threshold cycle (Ct) for evaluating test results
• IPC MTBC: added to each test and control sample at the lysis step of the DNA isolation procedure (exogenous internal positive control). The IPC serves as a control for the DNA isolation procedure and is used to monitor for the presence of PCR inhibitors

The VetMAX M. tuberculosis Complex PCR Kit is part of a workflow solution with the MagVet Universal Isolation Kit (Cat. No. MV384) and the Kingfisher Flex Magnetic Particle Processor or Kingfisher mL Flex Magnetic Particle Processor (Cat. Nos. 5400630 and 5400640).

TaqPath™ ProAmp™ Master Mix (Applied Biosystems™)

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Master Mix is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

Request an evaluation sample ›

Two TaqPath ProAmp Master Mix formulations for different throughput needs
• TaqPath ProAmp Master Mix—includes ROX passive reference dye for lower multiplexing of up to three targets
TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE passive reference dye for higher multiplexing of up to four targets

Benefits of TaqPath ProAmp Master Mix
Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock Taq DNA hot-start mechanism
Multiplexing breadth—two formulations that enable single to four-target detection per reaction
Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mix has been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. The figure below shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan genotyping assays using crude lysate from blood samples. TaqPath ProAmp Master Mix has been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High accuracy copy number quantitation
The versatile TaqPath ProAmp Master Mix can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. The figure below shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp Master Mix enables determination of copy number accurately in samples with up to four copies.

General purpose reagents
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled "For Laboratory Use."

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›

* Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.

Luminaris Color HiGreen qPCR Master Mix, high ROX (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

ABsolute Blue QPCR Mix, ROX (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with different ROX passive reference dye concentrations

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

Maxima Probe/ROX qPCR Master Mix (2X) (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima Probe/ROX qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima Probe/ROX qPCR Master Mix (2X)
Maxima Probe qPCR Master Mix (2X), with separate ROX vial

TaqMan™ Multiplex Master Mix (Applied Biosystems™)

Applied Biosystems® TaqMan® Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (<40 minutes) with excellent concordance of results in singleplex and multiplex reactions. TaqMan® Multiplex Master Mix contains AmpliTaq® Fast DNA PolymeraseUracil-N Glycosylase (UNG), dNTPs with dUTP, MUSTANG PURPLE® dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

Features of TaqMan® Multiplex Master Mix include:

• Optimized for multiplexing—validated for quadplex gene expression and genotyping assays
• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity
• Reduced run times on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
• Seamless integration into your experiments—validated with Applied Biosystems® TaqMan® assays and TaqMan® QSY® probes for gene expression and genotyping experiments
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility

Optimized for Multiplexing
Confidence is paramount when it comes to your results. TaqMan® Multiplex Master Mix is designed to deliver accurate results for multplex reactions and ensure excellent concordance of results in both singleplex and multiplex reactions.

Best-in-class Performance
TaqMan® Multiplex Master Mix is designed for performance equal to or better than other multiplex master mixes on the market. TaqMan® Multiplex Master Mix has been benchmarked against the leading suppliers' multiplex master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity.

Reduced Run Times on Standard Instrumentation
TaqMan® Multiplex Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides.

Carry-over Contamination Control
TaqMan® Multiplex Master Mix contains heat-labile uracil-DNA glycosylase (UDG) for worry-free carryover contamination control. The dUTP in the mix ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs. A UDG incubation step before PCR cycling destroys any contaminating dU-containing product from previous reactions. UDG is then inactivated by the high temperatures during normal PCR cycling, thereby allowing the amplification of genuine target sequences.

Instrument Compatibility
TaqMan® Multiplex Master Mix can be used in fast cycling mode on both standard and fast qPCR instruments, such as: 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System, ViiA™ 7 Real-Time PCR System, and QuantStudio™ 6 Flex, 7 Flex, and 12K Flex Real-Time PCR systems.

ABsolute Blue QPCR Mix, with separate ROX vial (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with different ROX passive reference dye concentrations

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

ABsolute QPCR Mix, SYBR Green, low ROX (Thermo Scientific™)

Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

Highlights

• High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

ABsolute QPCR Mix, low ROX (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Highlights

• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

Applications
• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

QuantStudio™ 12K Flex OpenArray™ Vaginal Microbiota Starter Kit (Applied Biosystems™)

A variety of starter kits are available to introduce you to the various applications of the QuantStudio™ 12K Flex OpenArray™ sample block. Each starter kit contains 2 arrays, plus sufficient reagents and accessories to perform two experiments. The QuantStudio™ 12K Flex OpenArray™ Vaginal Microbiota Starter Kit contains two training plates and a synthetic amplification control to serve as a sample for your experiments. This starter kit serves as a training tool for researchers new to this application.

The accessories provided in the kit include: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.

VetMAX™ PRRSV NA & EU Reagents (Applied Biosystems™)

Note: The name of this product has changed. It was previously called PRRSV REAGENTS W/PATCH EACH.

VetMAX™ NA and EU PRRSV Reagents contains primers/probes that enable multiplex, one-step, reverse transcription and real-time PCR (RT-PCR) targeting North American and European Porcine Reproductive and Respiratory Syndrome Virus (PRSSV) RNA and Xeno™ RNA Control. VetMAX™ NA and EU PRRSV Reagents contains an updated primer/probe mix compared to the similar TaqMan® NA and EU PRRSV Reagents.

• Rapid and cost-effective method for amplification of target sequences
• Quality reagents, manufactured under ISO 9001, providing confidence in the supply and quality of products
• Over 20 years in the DNA/RNA arena, with extensive bioinformatics capabilities and experience

PRSSV causes significant losses in the swine industry due to reproductive disorders, respiratory conditions, and growth retardation. PRRSV can be detected in blood and semen 24–48 hours after infection. Real-time PCR is the most sensitive method for monitoring RNA amplification.

TaqMan™ Universal Master Mix II, no UNG (Applied Biosystems™)

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Optimized buffer components

Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping Assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

Luminaris Color HiGreen qPCR Master Mix (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.

Luminaris Color Probe qPCR Master Mix, high ROX (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

VetMAX™-Plus qPCR Master Mix (Applied Biosystems™)

For use with VetMAX™ Reagents and VetMAX™ Controls. The VetMAX™-Plus qPCR Master Mix is designed for multiplex amplification of animal pathogen DNA targets using a rapid qPCR strategy. The kit includes Xeno™ DNA Control, which serves as an internal positive control for DNA purification and qPCR. DNA is amplified using AmpliTaq Gold® DNA Polymerase on a real-time PCR system.

ABsolute QPCR Mix, ROX (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.

Highlights

• High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
• Optimised buffer - For maximal performance in multiplex reactions

Applications
• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG (Applied Biosystems™)

TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-µL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

Convenience
Everything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility
TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

DyNamo ColorFlash Probe qPCR Kit (Thermo Scientific™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of DyNamo ColorFlash Probe qPCR Kit and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific DyNAmo ColorFlash Probe qPCR Kit offers equal performance to the DyNAmo Flash Probe qPCR Kit. In addition, it incorporates an innovative multicolor system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction set up, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

• Minimized risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2X master mix allows the use of UNG for prevention of carry-over contamination

Applications

• Fast qPCR
• qPCR using sequence-specific probes
• RT-qPCR using sequence-specific probes

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit

Platinum™ Quantitative PCR SuperMix-UDG w/ROX (Invitrogen™)

**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.

**********************
qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:


–Platinum® Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)

–Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
–ROX reference dye premixed at optimal concentration for ABI instruments


qPCR SuperMixes with ROX are available in two formats:

–Platinum® SYBR® Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR® Green I dye (Figure 2)
–Platinum® qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan®)


Contents and Storage:
Platinum® SYBR® Green qPCR SuperMix-UDG with ROX includes a 2X SuperMix with SYBR® Green I and ROX Reference Dyes, and 50 mM MgCl2. Platinum® qPCR SuperMix-UDG with ROX includes a 2X SuperMix with ROX Reference Dye, and 50 mM MgCl2. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.

ABsolute Blue QPCR Mix, SYBR Green, fluorescein (Thermo Scientific™)

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Includes
• ABsolute Blue qPCR SYBR Green Fluorescein Mix contains 2X master mix supplemented with fluorescein

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

TaqPath™ qPCR Master Mix, CG (Applied Biosystems™)

TaqPath qPCR Master Mix, CG, is designed for reliable, accurate quantification in gene expression and miRNA workflows. The master mix delivers confident results in both single and duplex reactions, even in the presence of inhibitors commonly found in clinical samples. All lots are functionally tested to help ensure lot-to-lot reproducibility for Ct consistency and dynamic range across a wide variety of assays. This General Purpose Reagent is manufactured according to applicable CFR requirements and labeled "For Laboratory Use". With stringent quality and premier performance, TaqPath qPCR Master Mix is a superior choice for your diagnostic testing or development needs.

Features of the TaqPath qPCR Master Mix include:
• Efficient and linear detection of up to eight logs with gene expression or miRNA assays*
• Enables reliable detection of low-copy templates with reproducible CT results
• Robust multiplexing performance with exogenous or endogenous targets
• Manufactured with stringent production and process controls to help ensure lot-to-lot consistency
• Labeled "For Laboratory Use"

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Validated with a breadth of workflows
TaqPath qPCR Master Mix, CG, has been validated to provide high specificity and dynamic range for use in multiple real-time PCR applications. The formulation can be run in either fast or standard cycling conditions on a wide variety of qPCR platforms. The figure below demonstrates the excellent PCR linearity over an 8-log input range of template with both gene expression and miRNA assays.

In addition, TaqPath qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate (figure below). TaqPath qPCR Master Mix has been benchmarked against similar competitor master mixes and demonstrates equivalent or better sensitivity and dynamic range across a variety of targets (figure below).

Reproducible sensitive detection
We understand the importance of reliably detecting low-copy targets for your test quality and data interpretation. TaqPath qPCR Master Mix, CG, helps generate significant and reproducible CT values for ≤10 copy detection. The figure below shows the consistent CT results obtained from three unique lots when detecting 10-copy inputs. This lot-to-lot CT consistency is preserved across multiple assays with different attributes and expression levels to maximize confidence in your results (figure below).

Optimized for multiplexing
Simultaneous amplification of multiple assays can be beneficial not only as a control to normalize and detect anomalies in experiments, but also to improve efficiency and cost-savings for labs. TaqPath qPCR Master Mix has been optimized with enzymes and component concentrations to facilitate multiplexing while preserving specificity, and it is validated for duplex performance in each manufacturing lot (figure below).

Inhibitor tolerant
Unlike other master mixes on the market, TaqPath qPCR Master Mix’s unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance to inhibitors compared to competitor mixes (figure below).

Manufacturing production and process controls
TaqPath qPCR Master Mix is manufactured under a quality management system that utilizes traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot.

General purpose reagent
For customers in the USA, TaqPath 1-Step RT-qPCR Master Mix is a General Purpose Reagent and "For Laboratory Use".

*Dynamic range is a property of both the assay and template concentration in the sample, as well as in the master mix, thus individual results may vary.