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ABsolute Blue QPCR Mix, SYBR Green, fluorescein (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

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Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

TaqPath™ ProAmp™ Multiplex Master Mix (Applied Biosystems™)

TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples. It is designed to deliver sensitive and confident results from genomic DNA targets on a broad range of qPCR instrument platforms. TaqPath ProAmp Multiplex Master Mix is manufactured under a strong ISO13485 quality management system and designed to produce consistent product performance lot after lot.

Request an evaluation sample ›

Two TaqPath ProAmp Master Mix formulations for different throughput needs
TaqPath ProAmp Master Mix—includes ROX passive reference dye for lower multiplexing of up to three targets
• TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE passive reference dye for higher multiplexing of up to four targets

Benefits of TaqPath ProAmp Master Mix
Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock Taq DNA hot-start mechanism
Multiplexing breadth—two formulations that enable single to four-target detection per reaction
Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mix has been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. The figure below shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan genotyping assays using crude lysate from blood samples. TaqPath ProAmp Master Mix has been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High accuracy copy number quantitation
The versatile TaqPath ProAmp Master Mix can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. The figure below shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp Master Mix enables determination of copy number accurately in samples with up to four copies.

General purpose reagents
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled "For Laboratory Use."

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›

* Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.

ABsolute Blue QPCR Mix, ROX (Thermo Scientific™)

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with different ROX passive reference dye concentrations

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

TaqMan™ Multiplex Master Mix (Applied Biosystems™)

Applied Biosystems® TaqMan® Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (<40 minutes) with excellent concordance of results in singleplex and multiplex reactions. TaqMan® Multiplex Master Mix contains AmpliTaq® Fast DNA PolymeraseUracil-N Glycosylase (UNG), dNTPs with dUTP, MUSTANG PURPLE® dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

Features of TaqMan® Multiplex Master Mix include:

• Optimized for multiplexing—validated for quadplex gene expression and genotyping assays
• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity
• Reduced run times on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
• Seamless integration into your experiments—validated with Applied Biosystems® TaqMan® assays and TaqMan® QSY® probes for gene expression and genotyping experiments
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility

Optimized for Multiplexing
Confidence is paramount when it comes to your results. TaqMan® Multiplex Master Mix is designed to deliver accurate results for multplex reactions and ensure excellent concordance of results in both singleplex and multiplex reactions.

Best-in-class Performance
TaqMan® Multiplex Master Mix is designed for performance equal to or better than other multiplex master mixes on the market. TaqMan® Multiplex Master Mix has been benchmarked against the leading suppliers' multiplex master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity.

Reduced Run Times on Standard Instrumentation
TaqMan® Multiplex Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides.

Carry-over Contamination Control
TaqMan® Multiplex Master Mix contains heat-labile uracil-DNA glycosylase (UDG) for worry-free carryover contamination control. The dUTP in the mix ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs. A UDG incubation step before PCR cycling destroys any contaminating dU-containing product from previous reactions. UDG is then inactivated by the high temperatures during normal PCR cycling, thereby allowing the amplification of genuine target sequences.

Instrument Compatibility
TaqMan® Multiplex Master Mix can be used in fast cycling mode on both standard and fast qPCR instruments, such as: 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System, ViiA™ 7 Real-Time PCR System, and QuantStudio™ 6 Flex, 7 Flex, and 12K Flex Real-Time PCR systems.

Luminaris Color Probe qPCR Master Mix, low ROX (Thermo Scientific™)

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe Low ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

TaqMan™ Reagents Starter Kit (Applied Biosystems™)

The TaqMan® PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan® 5' nuclease assay with the TaqMan® PCR Master Mix. Gene-specific, ready to use TaqMan® Gene Expression Assays and Universal Master Mix provide you with a fast, simple way for measuring changes in gene expression.

• Simple procedure gives you quick results
• Hot-Start DNA Polymerase enzyme system minimizes nonspecific product amplification for reliable results

Ready-to-Use Gene Expression Solution
The TaqMan® Reagents Starter Kit contains a sample of TaqMan® Universal PCR Master Mix, and TaqMan® Gene-Expression Assay (Eukaryotic 18S rRNA), plus a sample of human Raji cDNA - everything you need for 18S gene-expression analysis. Simply combine these reagents according to the instructions to generate reproducible and quantitative gene-expression data.

A Complete, Integrated Gene-Expression System
Eliminate time-consuming master mix formulation and assay optimization with the TaqMan® Gene-Expression Assays and the TaqMan® Universal PCR Master Mix, by using universal cycling conditions and pre-optimized reagents.

Used With
7500 Real-Time PCR System with Dell™ Notebook, 7500 Real-Time PCR System with Dell™Tower, 7900HT Fast Real-Time PCR System with 384-Well Block Module, 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory, 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module, 7900HT System Automation Accessory Upgrade, 7900HT System Standard 384-Well Block Upgrade Kit, 7900HT System Standard 96-Well Block Upgrade Kit, ABI PRISM® 7000 Sequence Detection System (Notebook).

For Research Use Only. Not for use in diagnostics procedures.

SYBR GreenER™ qPCR SuperMix Universal (Invitrogen™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR GreenER qPCR SuperMix and added additional capabilities for your gene expression analysis.

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SYBR® GreenER™ qPCR SuperMixes Universal incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments. The SYBR® GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR® Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR® GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR® GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR® GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR® GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

Eight cDNA targets and eight genomic DNA targets were amplified from 1 ng of cDNA and 6 pg of gDNA, respectively, on the ABI PRISM® 7900HT Sequence Detection System using the SYBR® GreenER™ qPCR SuperMix for ABI PRISM® instruments. For all 16 targets, detection was positive in the presence of template but negative for all no-template controls.

For Research Use Only. Not for use in diagnostics procedures.

Fast SYBR™ Green Master Mix (Applied Biosystems™)

Fast SYBR® Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR® Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq® Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR® Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.

TaqMan™ Universal Master Mix II, no UNG (Applied Biosystems™)

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Optimized buffer components

Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping Assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

ABsolute Blue QPCR Mix, with separate ROX vial (Thermo Scientific™)

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with different ROX passive reference dye concentrations

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute Blue QPCR Mix, low ROX
ABsolute Blue QPCR Mix, with separate ROX vial
ABsolute Blue QPCR Mix, ROX
ABsolute Blue QPCR Mix, SYBR Green, low ROX
ABsolute Blue QPCR Mix, SYBR Green, ROX
ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial
ABsolute Blue QPCR Mix, SYBR Green, fluorescein

TaqMan™ PreAmp Master Mix (Applied Biosystems™)

TaqMan® PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan® PreAmp Master Mix lets you:

• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE)
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan® Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Preamplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan® PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Easy Workflow
With TaqMan® PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan® assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan® PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp® 9700 thermal cycler.

VetMAX™ MastiType Micro4 Combo Kit (Applied Biosystems™)

This combo kit includes the VetMAX MastiType Micro4 qPCR Kit and the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria module (also available separately).

VetMAX MastiType Micro4 Kit
The VetMAX MastiType Micro4 assay enables rapid, accurate detection of the four major contagious mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen in a single reaction. The kit detects these four mastitis-causing pathogens:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Streptococcus uberis

Learn more about the VetMAX MastiType Micro4 Kit >

MagMAX CORE Nucleic Acid Purification Kit
The MagMAX CORE Nucleic Acid Purification Kit is a highly-effective solution for purifying RNA and DNA from an expansive range of veterinary sample types. The MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

Learn more about the MagMAX CORE Nucleic Acid Purification Kit >

MagMAX CORE Mastitis & Panbacteria Module
The MagMAX CORE Mastitis & Panbacteria Module is offered here in combination with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of all bacteria, especially those important in the detection of mastitis in cattle and from challenging matrices like mastitic milk.

We have included a specially optimized lysis solution, along with an adjusted extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram-positive bacteria.

Features of this combined product include:
Convenience—a complete solution using the same CORE chemistry
Easy—no centrifugation and no aspiration steps
Fast—less than 1 hour for 96 samples
Flexible—appropriate for fresh, frozen, or preserved milk samples, including samples with high somatic cell count

The MagMAX CORE kit and the MagMAX CORE Mastitis & Panbacteria Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.

VetMAX™ MastiType Micro4 Kit (Applied Biosystems™)

The VetMAX MastiType Micro4 Kit enables rapid, accurate detection of the four major contagious mastitis-causing pathogens in bovine milk using real-time PCR amplification of DNA unique to each pathogen in a single reaction.

The advanced technology of VetMAX MastiType real-time PCR assays offers several advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation

The VetMAX MastiType Micro4 assay detects these four pathogens in a single PCR reaction:
Staphylococcus aureus
Streptococcus agalactiae
Mycoplasma bovis
Streptococcus uberis

Luminaris HiGreen qPCR Master Mix, high ROX (Thermo Scientific™)

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: ingle copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen High ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

Path-ID™ qPCR Master Mix (Applied Biosystems™)

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.