Shop All miRNA qRT-PCR Kits

QuantStudio™ 12K Flex OpenArray™ Human miRNA Starter Kit (Applied Biosystems™)

A variety of starter kits are available to introduce you to the various applications of the QuantStudio™ 12K Flex OpenArray® sample block. Each starter kit contains 2 arrays, plus sufficient reagents and accessories to perform two experiments. The miRNA kit also contains cDNA controls to serve as samples for your experiments. Note that the QuantStudio™ 12K Flex Software (included with the QuantStudio™ 12K Flex System) offers example experiment files for each application.

The accessories provided with this kit include: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.

TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, fast (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced Human Endogenous Controls 96-Well Plate, Fast, provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains 1 plate than can be used to evaluate 30 potential endogenous control miRNAs for up to 3 samples. Two exogenous (non-human) controls are included for normalization of sample input.

Features of the TaqMan Advanced miRNA Human Endogenous Controls 96-Well Plate include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 1
Number of unique miRNAs (not including exogenous controls): 30
Exogenous controls: cel-miR-39-3p, ath-miR159a
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

TaqMan™ Advanced miRNA Human A 96-well Plates, standard (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human A 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human A 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA A Cards v2.0.

TaqMan™ Advanced miRNA Human A 96-well Plates, fast (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human A 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human A 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA A Cards v2.0.

mirVana™ qRT-PCR miRNA Detection Kit (Invitrogen™)

The mirVana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I.

• Quantitative results in less than 3 hours
• Detection from as little as 25 pg of total RNA
• Suitable for either real-time or end-point RT-PCR
• Ideal for validating expression profiling data and high-throughput applications.

MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNA molecules that are estimated to regulate at least 30% of all protein encoding genes. Currently more than 2,900 miRNAs have been annotated from a wide range of organisms including plants, worms, flies, mammals, and viruses. To date, miRNAs have been implicated in development, differentiation, apoptosis, fat metabolism, viral infection, and cancer.

Due to their small size (~22 nt), miRNAs cannot be analyzed by traditional PCR approaches. Designed for use in conjunction with qRT-PCR Primer Sets (not included), the mirVana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNAs, enabling accurate quantitation of miRNA expression levels (Figure 1). This novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I.

Since miRNAs represent only a minute fraction of total RNA, detection of these molecules can be difficult. However, the mirVana™ qRT-PCR miRNA Detection Kit is extremely sensitive, detecting miRNA from as little as 25 pg of total RNA. The kit also specifically detects mature miRNAs and not miRNA precursors (Figure 2). Furthermore, the mirVana™ qRT-PCR miRNA Detection Kit exhibits linearity over at least 5 logs (Figure 3) and can be used with total RNA, miRNA-enriched RNA fractions, or even degraded RNA samples.

Each kit contains sufficient reagents for 200 reactions, as well as a control primer set to amplify the ubiquitously expressed miR-24 and human heart total RNA. Note: Thermostable DNA polymerase is not supplied with the kit. For analysis of individual miRNAs, the mirVana™ qRT-PCR miRNA Detection Kit is unparalleled in sensitivity and speed!

TaqMan™ Advanced miRNA Human Serum/Plasma 96-well Plates, standard (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced Serum/Plasma 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 2
Number of unique miRNAs (not including exogenous controls): 188
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, standard (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced Human Endogenous Controls 96-Well Plate, Standard, provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains 1 plate than can be used to evaluate 30 potential endogenous control miRNAs for up to 3 samples. Two exogenous (non-human) controls are included for normalization of sample input.

Features of the TaqMan Advanced miRNA Human Endogenous Controls 96-Well Plate include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 1
Number of unique miRNAs (not including exogenous controls): 30
Exogenous controls: cel-miR-39-3p, ath-miR159a
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

TaqMan™ Advanced miRNA Human Serum/Plasma 96-well Plates, fast (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced Serum/Plasma 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 2
Number of unique miRNAs (not including exogenous controls): 188
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

TaqMan™ Advanced miRNA Human A and B 96-well Plates, standard (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human A and B 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 8 plates for profiling of up to 752 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human A and B 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 4 μL of purified plasma/serum or 2–20 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 8
Number of unique miRNAs (not including exogenous controls): 752
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

TaqMan™ MicroRNA Cells-to-CT™ Kit (Invitrogen™)

The Ambion® TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform microRNA (miRNA) expression profiling directly from cultured cells without RNA purification. The kit is the first to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

• Complete, validated solution—Optimized workflow includes cell lysis reagents, DNase, TaqMan® MicroRNA RT reagents, and TaqMan® Universal PCR Master Mix
• Fast, simple, and convenient—Prepare samples in 10 minutes; eliminate tedious RNA isolation
• Gold standard specificity—Achieve superior results with TaqMan® MicroRNA reagents
• Robust performance—Results equivalent to purified RNA
• Broad input dynamic range—Linear detection from 10 to 100,000 cells

Complete, Validated Solution

Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit contains TaqMan® MicroRNA reverse transcription (RT) reagents and TaqMan® Universal PCR Master Mix for real-time PCR analysis. The TaqMan® MicroRNA Cells-to-CT™ Kit has been extensively tested and validated with TaqMan® MicroRNA Assays, sold separately.

Simple, Convenient 10-Minute Sample Preparation

The TaqMan® MicroRNA Cells-to-CT™ Kit incorporates a simple 10-minute sample preparation procedure (Figure 1) for use with 10–100,000 cultured cells⁄sample, either in plates or tubes. Cells are washed in PBS and lysed for 8 minutes at room temperature; DNase treatment can be performed concurrently. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution.

The TaqMan® MicroRNA Cells-to-CT™ Kit lysates are now ready for reverse transcription or can be stored at -20°C for up to 5 months. Since samples are not subjected to an RNA purification process, all of the small RNA species are available for analysis. Because samples can be processed directly in culture plates (96 or 384 wells), sample handling is reduced, and the risk of sample loss or transfer error is minimized. No heating, washing, or centrifugation is required; the kit greatly simplifies a traditionally time-consuming, labor intensive process and reduces it to 10 minutes.

Gold Standard Specificity—Superior Results with TaqMan® MicroRNA Reagents

Applied Biosystems has developed a state-of-the-art solution to the fundamental problem associated with the short length of mature miRNAs. TaqMan® MicroRNA Assays (sold separately) include a unique, target-specific stem-loop reverse-transcription primer (Figure 2). The stem-loop structure is designed to incorporate specificity for the targeted mature miRNA, in contrast to other methods that indiscriminately extend and prime all RNA in a sample. The TaqMan® MicroRNA RT reagents, included in the TaqMan® MicroRNA Cells-to-CT™ Kit, were specifically designed to work in conjunction with this innovative assay design.

The ability to distinguish between highly homologous mature miRNA targets is required for accurate miRNA expression analysis. The TaqMan® MicroRNA Cells-to-CT™ Kit, in conjunction with the TaqMan® MicroRNA Assays, delivers the specificity needed to distinguish between highly homologous targets, as seen in Figure 3. In this example, lysates from the TaqMan® MicroRNA Cells-to-CT™ Kit or purified miRNA were used in real-time PCR using TaqMan® MicroRNA Assays to detect single base differences on exogenously added miRNA templates. Specificity obtained with TaqMan® MicroRNA Cells-to-CT™ Kit lysates (Figure 3A) was equivalent to that using purified RNA (Figure 3B).

Robust Performance—Results Equivalent to Purified RNA

This new kit has been extensively tested with a broad selection of TaqMan® MicroRNA Assays and demonstrates performance equivalent to that obtained with purified RNA (Figure 4). The TaqMan® MicroRNA Cells-to-CT™ Kit workflow enables unsurpassed miRNA expression evaluation with > 900 available TaqMan® MicroRNA Assays.

Broad Input Dynamic Range—Linear Detection from 10 to 100,000 Cells

Because miRNA expression levels can vary widely due to sample type, developmental stage, or disease progression, it is important to have sensitive tools for miRNA expression analysis. Now, miRNA targets that vary from a few copies to millions of copies can be accurately quantitated in the same experiment.

The TaqMan® MicroRNA Cells-to-CT™ Kit works robustly with as few as 10 cells, and up to 100,000 cells. Furthermore, to maximize sensitivity, the TaqMan® MicroRNA Cells-to-CT™ Kit allows up to 33% of the RT reaction volume to be comprised of sample lysate (singleplex RT). Figure 5 demonstrates the linearity and sensitivity of detection from TaqMan® MicroRNA Cells-to-CT™ Kit lysates across 5 logs of cellular input per lysis reaction; results were equivalent to purified RNA. Additionally, the broad dynamic range of TaqMan® MicroRNA Assays is not compromised by TaqMan® MicroRNA Cells-to-CT™ Kit lysates (data not shown).

Proven Performance, Proven Together

All components of the TaqMan® MicroRNA Cells-to-CT™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate kits for sample preparation, reverse transcription, and real-time PCR. The TaqMan® MicroRNA Cells-to-CT™ Kit has been validated with TaqMan® MicroRNA Assays for added quality assurance.

TaqMan™ Advanced miRNA Human A and B 96-well Plates, fast (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human A and B 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 8 plates for profiling of up to 752 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human A and B 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 4 μL of purified plasma/serum or 2–20 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 8
Number of unique miRNAs (not including exogenous controls): 752
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

TaqMan™ Advanced miRNA Human B 96-well Plates, standard (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human B 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human B 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA B Cards v3.0.

TaqMan™ Advanced miRNA Human B 96-well Plates, fast (Applied Biosystems™)

The Applied Biosystems TaqMan Advanced miRNA Human B 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human B 96-Well Plates include:
Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
Updated content—assay content updated for mirBase v.21
Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA B Cards v3.0.