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Click-iT™ TUNEL Colorimetric IHC Detection Kit (Invitrogen™)

The Click-iT™ TUNEL Colorimetric IHC Detection Kit is used to identify apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and streptavidin-peroxidase conjugation. After incorporation of the labeling moiety into sites of DNA fragmentation, detection is achieved through a catalyzed "click" reaction that adds a biotin group at these sites. The subsequent addition of a streptavidin-peroxidase and peroxidase substrate results in a dark brown signal that can be detected with light microscopy and stored for future analysis.

Find more tools for image-based detection of apoptotic cells >

• Optimized for the detection of apoptotic cells in either tissue or cell samples
• Improved colorimetric TUNEL assay—better label incorporation due to small reactive moiety
• Increased sensitivity—specific label incorporation results in lower background and brighter signal
• Content-rich results—cell morphology, cellular environment, and apoptotic signal are clearly visible
• Flexibility—the assay can be configured for 50 independent TUNEL apoptosis tests

The later stages of apoptosis are characterized by changes in nuclear morphology, chromatin condensation, nuclear envelope degradation, and DNA fragmentation. Terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assays are based on the incorporation of modified dUTPs by terminal deoxynucleotidyl transferase (TdT) at the 3’-OH ends of fragmented DNA. Colorimetric TUNEL assays utilize dUTPs conjugated with a biotin moiety, followed by the addition of a streptavidin-peroxidase conjugate and peroxidase substrate, resulting in a dark brown apoptotic signal. However, colorimetric TUNEL assays are prone to high background, which reduces the sensitivity and specificity of the signal.

The Click-iT TUNEL Colorimetric IHC Detection Kit was developed to address these issues by utilizing a dUTP modified with an alkyne group (a small bio-orthogonal functional group) that enables the nucleotide to be more readily incorporated by TdT. After incorporation, a highly specific click reaction covalently links biotin azide and the alkyne group. Streptavidin-peroxidase (horseradish peroxidase) followed by peroxidase substrate (DAB) are added, allowing colorimetric detection of apoptotic cells. The high degree of labeling specificity inherent in the click technology results in low background and improved detection of apoptotic cells.

The Click-iT TUNEL Colorimetric IHC Detection Kit has been optimized and contains all of the reagents needed for detection of apoptotic cells from either tissue or cell samples. The reagents supplied in this kit can be used to test 50 samples and configured to test from one to 50 samples at a time.

Amplex™ Red Cholesterol Assay Kit (Invitrogen™)

The Amplex® Red Cholesterol Assay Kit provides a sensitive, rapid, and simple fluorometric method for detecting very low concentrations of cholesterol using a fluorescence microplate reader or fluorometer. The assay measures both free cholesterol and cholesteryl esters.

See our complete line of Fluorescence Microplate assays.

 Detects as little as 200 nM (80 ng/ml) cholesterol
•  Helps accurately measure cholesterol content of 0.01 µl of human serum
•  Detects both free cholesterol and cholesteryl esters
•  Allows for multiple time point measurements
•  Designed for minimal autofluorescence interference

The Amplex Red Cholesterol Assay kit contains these reagents and buffers:


•  Amplex Red reagent
•  DMSO
•  Horseradish peroxidase
•  Hydrogen peroxide
•  Cholesterol esterase
•  Cholesterol oxidase
•  Cholesterol reference standard
•  Resorufin fluorescence reference standard
•  5X Reaction buffer

Cholesterol Measurement is Simple and Fast
Performing the Amplex® Red cholesterol assay is quick and easy, requiring only a 30-minute incubation after addition of reagents with no separation steps required. The 100 µl assay volume is designed for 96-well plates; fluorescence can be measured with a microplate reader or fluorometer. Since the assay is continuous, once the Amplex® Red reagent is added, multiple time-point measurements can be taken. The kit is complete with all necessary reagents except deionized water.

Helps Accurately Detect Very Low Cholesterol Concentrations
The Amplex® Red cholesterol assay utilizes enzyme-coupled reactions to produce highly fluorescent resorufin for quantitation. Resorufin is produced by the reaction of the Amplex® Red reagent with H2O2 produced from the cholesterol oxidase-catalyzed oxidation of cholesterol. Because a large proportion of cholesterol in blood is in the form of cholesteryl esters, cholesterol esterase is used to produce free cholesterol from cholesterol esters. Performing the assay in the presence and absence of cholesterol esterase potentially allows determination of the fractions of cholesterol in each form.

This highly sensitive method allows the Amplex® Red Cholesterol Assay kit to detect concentrations of cholesterol as low as 200 nM and measure the cholesterol content of 0.01 µl of human serum. Because the absorbance and emission maxima of resorufin are 571 nm and 585 nm respectively, autofluorescence in most biological samples does not interfere with the assay.

Use Amplex® Red Assays for a Broad Range of Investigations
A wide variety of validated Invitrogen Amplex® Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex® UltraRed (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence and the Amplex® Red/UltraRed stop reagent (Cat. No. A33855). The Amplex® Red/UltraRed stop reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours. Custom assay design and packaging are also available.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
Learn More about Amplex® Red Assays and Technology.

Arcturus™ Paradise™ PLUS QC Kit (Applied Biosystems™)

Formalin-fixed paraffin-embedded (FFPE) samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation of RNA molecules. Archival FFPE samples present additional challenges due to increased RNA degradation over time. As a result, not all FFPE samples contain high quality RNA.

The ARCTURUS® Paradise® PLUS Sample Quality Assessment Kit offers a simple solution for evaluating the quality of RNA from Formalin-Fixed Paraffin-Embedded (FFPE) tissues before proceeding with laser microdissection and downstream molecular analysis. The reagents provided in the kit are optimized to enable efficient extraction, isolation, and reverse transcription of total RNA from FFPE tissue scrapes. The RNA quantity and quality is then measured using quantitative real-time PCR (Figure 1).

The RNA quantity and quality are assessed by the presence of intact RNA for a specific message. Two sets of β-actin gene-specific primers designed to amplify short segments of DNA within the β-actin target sequence are used to estimate the amount of RNA in a given sample in relation to β-actin content using the real-time PCR technique. The RNA quantity derived from the 3' primer set is used to quantitate the RNA in the FFPE tissue scrape. The ratio of the RNA yield obtained from both sets of PCR primers is the 3'⁄5' ratio and is used as an indication of RNA quality.

Key product features:
• Secure Downstream Success with the Paradise® QC Kit
• Benefit from the Only Complete System for Formalin-fixed Samples

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from FFPE tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS®' Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

SelectFX™ Alexa Fluor™ 488 Endoplasmic Reticulum Labeling Kit, for fixed cells (Invitrogen™)

The SelectFX® Alexa Fluor® 488 Endoplasmic Reticulum Labeling Kit provides all the reagents needed to label the endoplasmic reticulum in fixed cells. To detect the ER, the kit uses a primary antibody directed against the ER-associated protein disulfide isomerase (PDI) and an Alexa Fluor® 488 dye-labeled secondary antibody. The fluorescence can be observed using the same optical filters used for fluorescein. The kit also includes cell fixative and permeabilization reagents and protocols for mammalian cell preparation and staining.

Kit Attributes:

Applications: Validated application for SelectFX® Alexa Fluor® 488 Endoplasmic Reticulum Labeling Kit is endoplasmic reticulum staining.
Format: Slide(s)
Detection Method: Fluorescence microscopy
Product Size: One kit

The network of folded membranes that comprises the endoplasmic reticulum (ER) in all eukaryotic cells constitutes greater than half of the total membrane in the average animal cell. The branching tubules and flattened sacs of the ER come in two forms: membrane bound by ribosomes (rough ER) or unbound by ribosomes (smooth ER). The ER has a central role in lipid and protein synthesis, protein chaperoning and folding, and calcium homeostasis. Abnormal protein folding in the ER is implicated in a number of diseases including cystic fibrosis and Alzheimer's disease.

The SelectFX® Alexa Fluor® 488 Endoplasmic Reticulum Labeling Kit provides all the reagents needed to label the ER in fixed cells. To detect the ER, the kit uses a primary antibody directed against the ER-associated protein disulfide isomerase (PDI) and an Alexa Fluor® 488 dye-labeled secondary antibody; fluorescence is observed using standard fluorescein filters. The kit also includes cell fixative and permeabilization reagents and protocols for mammalian cell preparation and staining.

Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Invitrogen™)

The Click-iT® Plus EdU Alexa Fluor® 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 488 nm laser of the flow cytometer. The Click-iT® Plus formulation is compatible with standard fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.

• Multiplexable—compatible with R-PE (and tandems) and fluorescent proteins
• Accurate—superior results compared to BrdU assays
• Fast—results in as little as 60 minutes

View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry.

Multiplexable
The Click-iT® Plus formulation provides increased multiplexibility compared to the original Click-iT® EdU Flow Cytometry assays. Click-iT® Plus EdU assays can be used in conjunction with R-PE and R-PE tandems, as well as fluorescent proteins such as GFP and mCherry, without loss of the accuracy or speed of the original Click-iT® EdU assay.

An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT® Plus EdU Flow Cytometry assay is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog that is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry, which is a copper-catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to the Alexa Fluor® dye. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population.

Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT® Plus detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it can be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution, as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use Click-iT® Plus EdU assay is compatible with cell cycle dyes. The Click-iT® Plus EdU assay can also be multiplexed with antibodies against surface and intracellular markers, as well as conjugates labeled with standard fluorophores including R-PE, R-PE tandems, and fluorescent proteins (GFP and mCherry).

Quick and Simple Protocol
The Click-iT® Plus EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling. However, EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:

1. Treat cells with EdU.
2. Fix and permeabilize cells.
3. Detect S-phase cells with Click-iT® Plus detection cocktail for 30 min.
4. Wash once.
5. Analyze.

Results can be seen in as little as 60 minutes in some circumstances, but we recommend 90 minutes for all applications.

Histogene™ LCM Frozen Section Staining Kit, includes dehydration chemicals, stain, and slides (Applied Biosystems™)

The Arcturus® HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for laser capture microdissection (LCM). Designed as a fast-penetrating stain, this kit provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs.

Key product features:
• Optimized process—provide superior staining while preserving RNA
• Comprehensive kit—simplify tissue staining and dehydration
• High-quality RNA yield—retain low-abundance mRNA and maintain RNA integrity
• Versatile application—obtain high-quality RNA from many tissue types
• Modular design—use with other Arcturus products to produce superior microarray data

Provide Superior Staining while Preserving Nucleic Acids
HistoGene® Staining Solution has been developed by Arcturus® to stain tissues for LCM subsequently used as sources of nucleic acids. It is a fast-penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, HistoGene® stain helps to preserve nucleic integrity that may be otherwise compromised when using longer staining protocols.

Simplify Tissue Staining and Dehydration
The HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for LCM.

Retain Low-abundance mRNA
RT-PCR analysis of specific genes from cells captured from tissues processed using the HistoGene® LCM Frozen Section Staining Kit shows retention of both low-abundance mRNA and higher-abundance species. The mRNA profile of HistoGene® samples appears free of degradation.

Maintain RNA Integrity
Tissues prepared for LCM using HistoGene® LCM Frozen Section Staining Kit reagents, supplies and instructions yield high quality RNA.

Obtain High-quality RNA from Many Tissue Types
Arcturus® has validated the HistoGene® Kit by examining RNA integrity on many tissue types. All tissues tested to date using the HistoGene® Kit have yielded quality RNA. Validated tissue types include (Table 1):

Part of the Arcturus® System for Microgenomics
The HistoGene® LCM Frozen Section Staining Kit is part of the Arcturus® Complete System of Microgenomics and is designed to seamlessly work together to produce high quality expression microarrays. With the PicoPure® RNA Isolation Kit, you can recover a high yield of RNA from as few as 10 cells in a minimal volume. The RiboAmp® RNA Amplification Kit can amplify picograms of your RNA sample into micrograms of amplified RNA (aRNA) that is ready for labeling and hybridization to microarrays. Arcturus® instruments and kits increase the reliability and reproducibility of gene expression studies.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, amino allyl (Applied Biosystems™)

The Arcturus® Paradise® PLUS Reagent System permits unprecedented gene expression analysis of millions of previously inaccessible samples. It is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

This kit provides reagents for 2-round in-vitro transcription, and aminoallyl aRNA labeling.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Simplified procedure – achieve unprecedented results in six steps
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent System enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries.

Achieve Unprecedented Results in Six Steps
Only six steps are required to take you from sample to results (See Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major microarray platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories (See Figures 2, 3, 4, and 5).

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (See Figure 6).

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise PLUS Reagent System is a validated as part of the Arcturus® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis. This system features the ArcturusXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. Arcturus Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular signatures otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

eBioscience™ Human Th17 Cytokine Staining Panel (Invitrogen™)

This human Th17 cytokine staining panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22. An anti-CD4 antibody that can be used after fixation and permeabilization, as well as IC Fixation and Permeabilization Buffers, is also included.

CD4+ T helper cells are critical mediators of the cellular immune response. For many years, due to cytokine expression patterns, it was thought that CD4+ T helper cells existed as a dichotomy of lineages named Th1 and Th2. However, further investigation revealed that the T helper cell population was not limited to these two subsets. Although it had long been appreciated that IL-17 (also known as IL-17A) production by T cells was required for protection against some pathogens, studies demonstrated that this cytokine was produced by a unique subset of T helper cells. Subsequent reports definitively showed that T cells could differentiate into IL-17-producing cells in vitro and in vivo independent of Th1 or Th2 development, thereby establishing Th17 cells as a unique T helper cell lineage. In addition to IL-17A expression, Th17 cells have been reported to express IL-17F (an heterodimers of IL-17A and Il-17F), IL-21 and IL-22. Functionally, Th17 cells play a role in host defense against extracellular pathogens by mediating the recruitment of neutrophils and macrophages to infected tissues. Moreover, it is becoming evident that aberrant regulation of Th17 cells may play a significant role in the pathogenesis of multiple inflammatory and autoimmune disorders.

Reactivity/Species
Human

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

eBioscience™ BrdU Staining Kit for Flow Cytometry PerCP-eFluor™ 710 (Invitrogen™)

The eBioscience BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.

This kit is optimized to achieve brighter staining compared to traditional protocols.

Reactivity/Species
Bovine, Canine, Cow, Human, Monkey, Mouse, Non-human primate, Rat, Rhesus

Conjugate
PerCP-eFluor 710

Laser
Blue Laser

Emit
710 nm

Excite
488 nm

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

SuperPicture™ Polymer Detection Kit, HRP, broad spectrum

The SuperPicture™ Polymer Detection kit features our proprietary and enhanced HRP polymer technology to provide excellent sensitivity, high specificity, and a shorter
protocol than conventional immunohistochemical staining methods.

Our new polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. The polymer conjugate contains multiple HRP molecules to provide excellent sensitivity. The polymer staining method is faster and easier than a conventional LAB-SA protocol because it uses an HRP polymer conjugated to the second antibody and eliminates one incubation step. In addition, SuperPicture™ Polymer kits do not contain biotin nor streptavidin, so potential background due to endogenous biotin activity is completely avoided. Another advantage of SuperPicture™ Polymer detection system is the shorter polymer incubation time; the this system only requires 10 minutes, while leading competitors’ polymers require 30 minutes.

Reactivity: Mouse, Rabbit, Rat, G. Pig

Applications: Immunohistochemistry (FFPE)

Vybrant™ MTT Cell Viability Assay (Invitrogen™)

The Vybrant MTT Cell Viability Assay utilizes the well-established and widely used MTT reagent to determine mammalian cell viability. The redox potential in active mammalian cells reduces MTT to a strongly pigmented formazan product. After solubilization, the absorbance of the formazan can be measured with a microplate absorbance reader. The Vybrant MTT Cell Viability Assay is a complete, optimized kit that provides all the reagents necessary for the detection of mammalian cell viability. The kit provides a sufficient amount of material for ~1000 assays.

More tools for microplate-based detection of viability ›

Features of the Vybrant MTT Cell Viability Assay include:
• Complete and optimized kit for colorimetric detection of viable mammalian cells
• Utilizes the well-established MTT reagent as reporter of cellular redox potential
• Configured to perform 1,000 tests using a 96-well microplate

Measuring changes to cell viability is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. Several methods can be used for such determinations, but procedures using colorimetric indicators provide a rapid and cost-effective method for determining changes to mammalian cell viability. Among the various colorimetric viability assays, the MTT assay is a well-established and popular assay.

The redox potential in viable mammalian cells causes the water soluble MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to convert to an insoluble formazan product. After solubilization of the formazan with the included SDS (sodium dodecyl sulfate) reagent, the concentration of the colorimetric probe is determined by an optical density measurement at 570 nm.

The Vybrant MTT Cell Viability Assay provides a simple method for determination of mammalian cell viability using standard microplate absorbance readers. Simply prepare the MTT reagent, add it to the cells, solubilize the resulting formazan, and determine the optical density using a standard microplate reader. The Vybrant MTT Cell Viability Assay provides the necessary materials to perform 1,000 individual tests using standard 96-well microplates.

SelectFX™ Alexa Fluor™ 488 Peroxisome Labeling Kit, for fixed cells (Invitrogen™)

The SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit provides all the reagents needed to label the peroxisomes in fixed cells and includes cell fixative and permeabilization reagents. For detection, the kit uses a primary antibody directed against peroxisomal membrane protein 70 (PMP 70), which is a high-abundance integral-membrane component of peroxisomes, and an Alexa Fluor® 488 dye-labeled secondary antibody. The fluorescence can be observed using the same optical filters used for fluorescein.

Applications: peroxisome staining
Product size: 1 kit pack size

Peroxisomes are single membrane-bound vesicles that are found in most eukaryotic cells. Their chief function is to enzymatically oxidize fatty acids and to subsequently catalyze the breakdown of H2O2, a by-product of fatty acid degradation. Recently, interest in peroxisomes has increased especially in studies related to peroxisomal origin and maintenance. Morphological abnormalities in peroxisomes related to disease states and diet have also been on the forefront of current research. The SelectFX® Alexa Fluor ® 488 Peroxisome Labeling Kit provides all the reagents needed to label the peroxisomes in fixed cells, and includes cell fixative and permeabilization reagents. For detection, the kit uses an antibody directed against peroxisomal membrane protein 70 (PMP 70), which is a high-abundance integral-membrane component of peroxisomes. PMP 70 is significantly induced by administration of hypolipidemic agents in parallel with peroxisome proliferation and the induction of peroxisomal fatty acid beta-oxidation enzymes.

pHrodo™ Red Zymosan Bioparticles™ Conjugate for Phagocytosis (Invitrogen™)

New pH-sensitive pHrodo® Red dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo® Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.

• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein

The fluorescence of the novel pHrodo® Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo® Red Zymosan BioParticles® Conjugate in imaging or flow applications, or pHrodo® Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.

In addition, pHrodo® dye is also available in green color, with E. coli, zymosan, S. aureus, and dextran 10,000 conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Histogene™ Staining Solution (Applied Biosystems™)

This staining solution is for use with the ARCTURURS® HistoGene® LCM Frozen Section Staining Kit.

Designed as a fast-penetrating stain, it provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs in frozen tissue sections intended for laser capture microdissection (LCM).

For Research Use Only. Not for use in diagnostics procedures.

Amplex™ Red Phosphatidylcholine-Specific Phospholipase C Assay Kit (Invitrogen™)

The Amplex® Red Phosphatidylcholine-Specific Phospholipase C Assay Kit provides a sensitive method for detecting phosphatidylcholine-specific phospholipase C (PC-PLC) activity using a fluorescence microplate reader or fluorometer.

See our complete line of Fluorescence Microplate assays.

• Detects phospholipase-C activity levels as low as 0.2 mU/mL
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference

In this enzyme-coupled assay, PC-PLC activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex® Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. PC-PLC converts phosphatidylcholine (lecithin) to phosphocholine and diacylglycerol. Alkaline phosphatase is added, which hydrolyzes phosphorylcholine to form choline. Choline is then oxidized by choline oxidase to form betaine and hydrogen peroxide. In the presence of horseradish peroxidase, the hydrogen peroxide reacts with Amplex® Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.

Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.

Use Amplex® Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex® Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex® UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex® Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex® Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.