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eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 570 (Invitrogen™)

This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with immunofluorescence detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using a streptavidin-conjugated fluorophore. This kit has been optimized for IHC with both frozen and formalin-fixed paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Conjugate
eFluo 570

Emit
570 nm

Excite
555 nm

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunohistochemical Staining of Frozen Tissue Sections, Immunocytochemistry, Microscopy

ABC Peroxidase Standard Staining Kit (Thermo Scientific™)

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit
Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit

Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit (Thermo Scientific™)

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit

eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 660 (Invitrogen™)

This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with immunofluorescence detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using a streptavidin-conjugated fluorophore. This kit has been optimized for IHC with both frozen and formalin-fixed paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Conjugate
eFluor 660

Laser
Red Laser

Emit
668 nm

Excite
633 - 647 nm

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunohistochemical Staining of Frozen Tissue Sections, Immunocytochemistry, Microscopy

Ultra-Sensitive ABC Peroxidase Standard Staining Kit (Thermo Scientific™)

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit
Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit

SuperPicture™ Polymer Detection Kit, HRP, broad spectrum

The SuperPicture™ Polymer Detection kit features our proprietary and enhanced HRP polymer technology to provide excellent sensitivity, high specificity, and a shorter
protocol than conventional immunohistochemical staining methods.

Our new polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. The polymer conjugate contains multiple HRP molecules to provide excellent sensitivity. The polymer staining method is faster and easier than a conventional LAB-SA protocol because it uses an HRP polymer conjugated to the second antibody and eliminates one incubation step. In addition, SuperPicture™ Polymer kits do not contain biotin nor streptavidin, so potential background due to endogenous biotin activity is completely avoided. Another advantage of SuperPicture™ Polymer detection system is the shorter polymer incubation time; the this system only requires 10 minutes, while leading competitors’ polymers require 30 minutes.

Reactivity: Mouse, Rabbit, Rat, G. Pig

Applications: Immunohistochemistry (FFPE)

eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 615 (Invitrogen™)

This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with immunofluorescence detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using a streptavidin-conjugated fluorophore. This kit has been optimized for IHC with both frozen and formalin-fixed paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Conjugate
eFluor 615

Emit
615 nm

Excite
595 nm

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunohistochemical Staining of Frozen Tissue Sections, Microscopy, Immunocytochemistry

ELF™ 97 Immunohistochemistry Kit (Invitrogen™)

The ELF® 97 Immunohistochemistry Kit can be used to detection biotinylated probes including secondary antibodies. The kit also includes the ELF® 97 phosphatase substrate that upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift, 180 nm that makes it easily distinguishable from endogenous fluorescence.

Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit (Thermo Scientific™)

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit

eBioscience™ BrdU Kit for IHC/ICC Colorimetric (Invitrogen™)

This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with colorimetric detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using an Avidin-HRP (horseradish peroxidase) and DAB (3, 3'-Diaminobenzidine) substrate reaction. This kit has been optimized for IHC with both frozen and paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Microscopy, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections

Metal Enhanced DAB Substrate Kit (Thermo Scientific™)

Thermo Scientific Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.

Features of the Metal-Enhanced DAB Substrate Kit :

Incredible sensitivity—fifty times more sensitive than the traditional DAB method and thiry times more sensitive than other metal-intensified versions of DAB
Low background, high intensity—get a crisp dark brown-black precipitate that is more intense than the dull brown precipitate when using DAB without enhancement. And, even with the increased intensity, background is almost nonexistent
Only two components—simply mix the two liquid components and your working solution is ready to use
Six-hour stability—the innovative working solution is stable for more than six hours at room temperature, while other DAB substrates must be used immediately

To prepare the product, the 10X Metal Enhanced Substrate Solution is diluted with the Pierce Stable Peroxide Buffer prior to use, and the special formulation allows the 1X substrate to be stable for at least 6 hours at room temperature. The background color of the substrate does not increase and, unlike typical metal enhanced DAB methods, the reactivity is unchanged (1,2). The resulting precipate is a dark brown-black that is more intense than the brown color from DAB alone. The intensity not only increases, but the background is also reduced, which increases the signal-to-noise ratio and makes screening tissue or membrane antigens easier.

Pierce™ Peroxidase IHC Detection Kit (Thermo Scientific™)

The Thermo Scientific Pierce Peroxidase IHC Detection Kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counterstain and preserve experimental results with frozen or paraffin tissue sections.

Features of the Peroxidase IHC Detection Kit:

Incredibly sensitive—fifty times more sensitive than the traditional DAB (3,3'-diaminobenzidine) method and 30 times more sensitive than other metal-intensified versions of DAB
Low background, high intensity, large dynamic range—gives a crisp dark brown-black precipitate that's more intense than the dull brown precipitate observed when using DAB without enhancement; substrate delivers increased intensity with almost nonexistent background
Easy-to-use, two-component system—mix the two liquid components and the working solution is ready-to-use; sufficient substrate and other staining reagents to treat up to 500 slides
Six-hour working solution stability—unlike other DAB substrates that must be used immediately, the working solution is stable for more than six hours at room temperature
Complete kit—saves time in reagent ordering, gathering and validating reagents for the IHC application; saves money compared to purchasing of all components separately

This immunohistochemical staining kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counter-stain and preserve experimental results with frozen or paraffin tissue section that have been probed with peroxidase enzyme conjugates. The Pierce Peroxidase IHC Detection Kit eliminates the need to procure numerous reagents from various sources, resulting in a time- and cost-effective way to perform peroxidase-based tissue staining. Harris Hematoxylin nuclear stain and mounting medium allow counter-staining and tissue preservation, respectively. The detection method is compatible with direct or avidin-biotin-complex (ABC) probing strategies.

Applications:
• Tissue immunohistochemistry procedures designed for DAB precipitating substrate
• DAB staining of fixed paraffin sections, smears and frozen section preparations
• Immunohisto- or immunocyto-staining using the metal-enhanced DAB substrate

Histostain-Plus IHC Kit, rabbit

Full Product Description
Histostain-Plus kits use 2nd generation LAB-SA technology and are ideal for general immunohistochemical applications. Histostain-Plus kits offer superior sensitivity without compromising background. They are designed to detect mouse, rabbit, rat, or guinea pig primary antibodies that are bound to human tissue or cell antigens. Histostain-Plus kits are excellent for frozen and formalin-fixed, paraffin-embedded tissue sections, cell smears, etc. These kits are very easy to use: all components are liquid (except Fast-Red) and come in dropper bottles. No reconstitution, no pipetting, no measuring is necessary, and the enzyme is conjugated to streptavidin to eliminate the preincubation complexing step. DAB chromogen yields a brown stain, AEC yields a deep red stain, and Fast-Red yields a bright red stain.

Reactivity: Rabbit

Applications: Immunohistochemistry (IHC)