Shop All Cell Vitality Assay Kits

LIVE/DEAD™ Violet Viability/Vitality Kit, for 405 nm excitation (Invitrogen™)

The LIVE/DEAD™ Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.

View a selection guide for all Nonfixable Viability Dyes for Flow Cytometry.

LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green (Invitrogen™)

The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.

View a selection guide for all Cell Viability Assays for Flow Cytometry.

BacLight™ RedoxSensor™ Green Vitality Kit (Invitrogen™)

The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. This reductase activity is, in turn, a reliable marker for changes in electron transport chain function and for changes in vitality that occurs following antibiotic treatment. RedoxSensor™ Green reagent penetrates both gram-positive and gram-negative bacteria. Following reduction, the RedoxSensor™ Green reagent will produce a stable green-fluorescent signal in 10 minutes that is compatible with formaldehyde fixation techniques.

View additional information about all microbiology assays for flow cytometry.

FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit (Invitrogen™)

The FungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

BacLight™ RedoxSensor™ CTC Vitality Kit (Invitrogen™)

The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product. Cells not respiring or respiring at slower rates will reduce less CTC and consequently produce less fluorescent product, giving a semi-quantitative estimate of healthy vs. unhealthy bacteria. Green- and blue-fluorescent nucleic acid stains are included as counterstains to assist differentiation of cells from debris and calculating total cell numbers.

View additional information about all microbiology assays for flow cytometry.