Shop All Cell Viability Kits

PrestoBlue™ Cell Viability Reagent (Invitrogen™)

Get high quality cell viability results and instant time savings with PrestoBlue® Cell Viability Reagent. PrestoBlue® is a ready to use cell permeable resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells. When added to cells, the PrestoBlue® reagent is modified by the reducing environment of the viable cell and turns red in color, becoming highly fluorescent. This color change can be detected using fluorescence or absorbance measurements.

The PrestoBlue® Cell Viability Reagent allows you to:

• Save time with an incubation step as short as 10 minutes.
• Obtain high quality results with a large dynamic range
• Experience convenience with the add and read homogenous format
• Monitor living cells and perform downstream functional assays


10 Minute Incubation
The PrestoBlue® Cell Viability Reagent is the only live-cell viability reagent on the market able to provide robust data typically in as little as 10 minutes following reagent addition. All other resazurin-based cell viability reagents on the market require a one to four hour incubation with the reagent prior to obtaining data.

High Quality Results
Based on the cell type and incubation time, PrestoBlue® Cell Viability Reagent can detect as few as 12 cells per well in a 384 well plate. After ten minutes of incubation the PrestoBlue® Reagent can linearly detect cells down to 98 cells⁄well with a Z’ value of greater than 0.5.

Homogenous Assay Format
The PrestoBlue® Cell Viability Reagent is an addition-only reagent. Without the cell lysis or solubilization steps required by other assays on the market, hands-on time is minimized.

Live Cell Assay
The PrestoBlue® Cell Viability Reagent does not require cell lysis. Cells may be incubated for up to 24 hours in the presence of PrestoBlue® Cell Viability Product. Simply remove the reagent from the cells and replace it with growth medium for further proliferation.

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells (Invitrogen™)

The LIVE/DEAD® Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.

Advantages Over Alternative Methods Include:


•  Faster
•  Safer
•  More sensitive
•  Less expensive

Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD® Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.

Sensitive, Safe, and Efficient

The LIVE/DEAD® Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD® Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.

LIVE/DEAD® Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD® Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD® assays provide quick, positive discrimination between viable and non-viable cells.

Related Link
•  Learn More and See the Full Range of LIVE/DEAD® Assay Products.

LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit, for flow cytometry (Invitrogen™)

The LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of microspheres for accurate sample volume measurements.

View additional information about all microbiology assays for flow cytometry.

Tali™ Viability Kit - Dead Cell Red (Invitrogen™)

The Tali® Viability Kit - Dead Cell Red is a ready-to-use solution of propidium iodide (PI) that has been validated for use with the Tali® Image-Based Cytometer. PI is a live cell-impermeant fluorogenic DNA-binding dye that has been extensively used to identify necrotic cells.

Key Features of Tali® Viability Kit - Dead Cell Red:

• Impermeant to live cells
• Easily enters and binds to nucleic acids in necrotic cells

After staining a cell population using Dead Cell Red™, dead cells display red fluorescence upon excitation. Tali® Viability Kit - Dead Cell Red can be used to identify dead cells in a population of unstained cells, or in a population of GFP-expressing cells.

LIVE/DEAD™ Fixable Dead Cell Stain Sampler Kit (Invitrogen™)

LIVE/DEAD™ Fixable dead cell stains are used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The stains in the sampler kit have been optimized and validated for use in flow cytometry.

• Stable—dyes are freeze-dried in separate vials to maintain stability
• Robust—staining pattern is the same before and after fixation
• Bright signal—allows for easy distinction between live/dead cells in single channel

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable dead cell stains in this sampler kit have been conveniently packaged in eight 40-test vials to help ensure the stability and performance of the dye over time. Amine-reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to use the vial completely once rehydrated or aliquot the solution and store at -80°C, avoiding freeze thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with formaldehyde or ethanol-based fixatives required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable dead cell stains are amine-reactive dyes that bind covalently to intracellular and extracellular amines, allowing the staining pattern to be preserved following formaldehyde fixation.

Optimal brightness
The LIVE/DEAD™ Fixable dead cell stains were selected based on their fluorescent properties to provide a bright signal when excited with the optimal lasers. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, these dead cell stains are an ideal choice for multi-color experiments.

How it works
In cells with compromised membranes, the dead cell stain reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells allowing for easy discrimination.

Colors available
LIVE/DEAD™ Fixable dead cell stains are available in a wide variety of colors to meet your multi-color panel needs. The sampler kit provides one vial (40 assays) of each of the eight dead cell stains available.

Find the LIVE/DEAD™ Fixable Dead Cell Stain that fits your needs:

• For UV lasers: blue
• For violet lasers: aqua, violet, or yellow
• For blue lasers: green or red
• For red lasers: far-red or near-IR

ReadyProbes™ Cell Viability Imaging Kit, Blue/Green (Invitrogen™)

ReadyProbes® Cell Viability Imaging Kit (Blue/Green) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of room temperature, stable NucBlue® Live reagent (Hoechst 33342) and NucGreen® Dead reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue® Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen® Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set. This kit is appropriate for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

NucBlue® Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm)
NucGreen® Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523 nm)
See other ReadyProbes® reagents for cell staining
Learn more about other assays for cell viability

Suggestions for use
• NucBlue® Live and NucGreen® Dead reagents may be added directly to cells in full growth media or a compatible buffer solution.
• In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.

alamarBlue™ HS Cell Viability Reagent (Invitrogen™)

alamarBlue HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. High background fluorescence caused by various contaminants reduces the performance of resazurin-based reagents. To improve the performance, an innovative purification process was developed that removes these contaminants. This highly purified, non-toxic resazurin was used in the standard alamarBlue formulation, creating the multiple-component alamarBlue HS Cell Viability Reagent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. The alamarBlue HS Cell Viability Reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

alamarBlue HS Cell Viability Reagent features include:
Removal of contaminants from resazurin—displays a >50% reduction in background fluorescence
Signal-to-background ratio increased by >100%—results in large assay signal window
Highly sensitive reagent with a linear response—detects as few as 20 cells per well
Convenient add-and-read format—no mixing, no washing, no cell lysis, and compatible with either fluorescence- or absorbance-based instrumentation
Measures viability from many diverse cell types—including mammalian cells, bacteria, plant, and fungi

Measuring changes in cell viability is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. Cell health can be monitored by detecting changes in several key indicators, including changes to plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and cellular reducing environment.

Monitoring changes to the cellular reducing environment or metabolic activity by using resazurin-based reagents is a well-established and reliable indicator of cell viability or death. Upon entering living cells, the cellular reducing environment reduces resazurin to resorufin, a compound that is red in color and highly fluorescent. Viable cells continuously convert resazurin to resorufin, increasing the overall fluorescence of the medium surrounding the cells. Additionally, the conversion of resazurin to resorufin results in a pronounced color change, thus cell viability can also be detected using absorbance-based plate readers.

As a consequence of the synthesis and manufacturing processes, all resazurin-based reagents contain a detectable amount of resorufin contamination. The amount of contamination can vary greatly between sources of the material and manufacturing conditions, contributing to differences in detectable background fluorescence. More importantly, the contaminating resorufin reduces the signal to background ratio and dynamic range of the assay. An innovative process was developed that removes the contaminating resorufin, resulting in the highly pure resazurin used in alamarBlue HS Cell Viability Reagent.

alamarBlue HS Cell Viability Reagent is a complete add-and-read, non-toxic reagent that does not require cell lysis. The highly purified resazurin used for alamarBlue HS reagent results in a >50% decrease in background fluorescence and a >100% increase in the signal-to-background ratio. Since no lysis is required, the diluted alamarBlue HS solution can be removed and replaced with complete growth medium for further culturing of the cells. Similar to alamarBlue reagent, alamarBlue HS reagent displays an extended viability detection time window for a wide variety of organisms and thus can be used with various human and animal cell lines, bacteria, plant, and fungi, resulting in quantitative measurement of cell viability and proliferation.

LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy (Invitrogen™)

The LIVE/DEAD BacLight Bacterial Viability Kit *for microscopy* is a convenient and easy-to-use kit for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell. Cells with a compromised membrane that are considered to be dead or dying will stain red, whereas cells with an intact membrane will stain green.

LIVE/DEAD™ Sperm Viability Kit (Invitrogen™)

The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm by fluorescence microscopy or flow cytometry. Membrane-permeant SYBR® 14 nucleic acid stain labels live sperm with green fluorescence, and membrane-impermeant propidium iodide labels the nucleic acids of membrane-compromised sperm with red fluorescence.

LIVE/DEAD™ Reduced Biohazard Cell Viability Kit #1, green & red fluorescence (Invitrogen™)

The LIVE/DEAD Reduced Biohazard Cell Viability Kit #1 is a convenient and easy-to-use cell viability kit that is designed to reduce the risk associated with handling potential biohazards such as viral, bacterial or protozoan pathogens. The assay monitors viability as a function of the membrane integrity of the cell. Cells with a compromised membrane that are considered to be dead or dying will stain red, whereas cells with an intact membrane will stain green.

LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation (Invitrogen™)

The LIVE/DEAD™ Fixable Far-Red Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a red laser flow cytometer.

• Stable—dyes are freeze dried in separate vials to maintain stability

• Robust—staining pattern is the same before and after fixation

• Bright signal—allows for easy distinction between live/dead cells in single channel

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Far-Red Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Far-Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Optimal brightness
The LIVE/DEAD™ Fixable Far-Red Stain was selected based on its fluorescent properties to provide a bright signal when excited with a red laser. The far-red fluorescent reactive dye has an excitation maximum of ~633 nm making it ideal for use with the red or HeNe laser with an emission of ~655 nm. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.

How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.

Pierce™ LDH Cytotoxicity Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce LDH Cytotoxicity Assay Kit is a reliable colorimetric assay to quantitatively measure lactate dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis.

Features of the LDH Cytotoxicity Assay Kit:

Convenient—add-mix-read assay format for adherent and suspension cells
Colorimetric—quantitatively measures LDH release by formation of colored product
Robust—uses stable LDH enzyme activity as a cytotoxic marker
Flexible—ideal for high-throughput screening
Non-radioactive—safe alternative to 51Cr-release cytotoxicity assays

The Pierce LDH Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays.

Includes:
Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control

Requires:
Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96-well plate compatible with spectrophotometer, multichannel pipette

Applications:
• Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
• Detect cytolysis in bioreactor or 3D tissue engineering applications

Lactate dehydrogenase (LDH) is a cytosolic enzyme present in many different types of cells. When the plasma membrane is damaged, LDH is released into cell culture media. The released LDH can be quantified by a coupled enzymatic reaction. First, LDH catalyzes the conversion of lactate to pyruvate via reduction of NAD+ to NADH. Second, diaphorase uses NADH to reduce a tetrazolium salt (INT) to a red formazan product. Therefore, the level of formazan formation is directly proportional to the amount of released LDH in the medium.

The assay is performed by transferring cell culture media from treated cells into a new microplate and adding the kit reagents. After incubation at room temperature for 30 minutes, reactions are stopped and LDH activity is determined by spectrophotometric absorbance at 490nm.

BacLight™ Green Bacterial Stain (Invitrogen™)

The BacLight Green (Cat. no. B-35000) and BacLight Red (Cat. no. B-35001) bacterial stains are fluorescent labeling reagents for detecting and monitoring bacteria. These two dyes are not nucleic acid stains. Bacteria stained with the BacLight Green and BacLight Red bacterial stains exhibit bright green and red fluorescence (absorption/emission ~480/516 and ~581/644 nm, respectively), and can be resolved using the appropriate flow cytometric channels or fluorescence microscopy filters. The BacLight bacterial stains are compatible with formaldehyde or alcohol fixation methods.

View additional information about all microbiological analysis products.

CellROX™ Deep Red Flow Cytometry Assay Kit (Invitrogen™)

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX® Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.

LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation (Invitrogen™)

The LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a UV laser flow cytometer.

• Stable—dyes are freeze dried in separate vials to maintain stability
• Robust—staining pattern is the same before and after fixation
• Bright signal—allows for easy distinction between live/dead cells in single channel

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Blue Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Optimal brightness
The LIVE/DEAD™ Fixable Blue Stain was selected based on its fluorescent properties to provide a bright signal when excited with a UV laser. The blue-fluorescent reactive dye has an excitation maximum of ~350 nm, making it ideal for use with an UV laser, and an emission of ~450 nm. By switching viability staining from a channel off of the blue 488 nm laser to the UV laser, you will free up a channel on your flow cytometer for other reagents that are difficult to find in colors other than FITC or PE. By using the UV laser, there is no spectral overlap issues with other common dyes, thus eliminating the need to compensate the viability stains from other stains in your panel. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.

How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.