Shop All HCS Detection Kits

HCS CellMask™ Near-IR Stain (Invitrogen™)

HCS CellMask Near-IR Stain is a cell-delineation tool for HCS platforms. Members of the HCS CellMask family of stains label the entire cell (nucleus and cytoplasm) and provide an accurate backdrop against which features of interest can be assessed. These stains can be applied to cells immediately after fixation or during the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If nuclear staining only is desired, HCS NuclearMask stains are available and can be used for measuring DNA content in addition to enabling robust cell demarcation.

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HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit, for high-content screening, for cellular imaging (Invitrogen™)

HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. The kit supplies LipidTOX™ Red phospholipid stain and LipidTOX™ Green neutral lipid stain, which can be used sequentially for the analysis of phospholipidosis and steatosis, respectively, or can be used in isolation for single-parameter analysis. Both fluorescent probes can be easily detected with fluorescence microscopes and can be quantified with stand-alone image analysis software or the built-in image analysis software of most HCS readers. In addition to the phospholipid and neutral lipid stains, this kit supplies the nuclear stain Hoechst 33342, propranolol and cyclosporin A (control compounds), and DMSO. Sufficient reagents are supplied for 2, 96-well plates (H34157) or 10, 96-well plates (H34158).

HCS Mitochondrial Health Kit (Invitrogen™)

The HCS Mitochondrial Health Kit simultaneously and quantitatively measures two important cell-health parameters: mitotoxicity and cytotoxicity. The MitoHealth stain detects changes in the mitochondrial membrane potential, whereupon the reagent accumulates in active mitochondria. The kit includes the Image-iT® DEAD™ Green to detect cytotoxicity, an impermeant dye to healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.

HCS LipidTOX™ Green Phospholipidosis Detection Reagent (1000X), for cellular imaging (Invitrogen™)

The HCS LipidTOX™ Green phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima ~495/525 nm. LipidTOX™ phospholipidosis detection reagents do not affect the normal growth of cells, and the staining observed after incubation in live cells is maintained after formaldehyde fixation. This probe is compatible with the HCS LipidTOX™ neutral lipid stains (H34475, H34476, and H34477).

HCS LipidTOX™ Red Phospholipidosis Detection Reagent (1000X), for cellular imaging (Invitrogen™)

The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima ~595/615 nm. LipidTOX™ phospholipidosis detection reagents do not affect the normal growth of cells, and the staining observed after incubation in live cells is maintained after formaldehyde fixation. This probe is compatible with the HCS LipidTOX™ neutral lipid stains (H34475, H34476, and H34477).

Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay (Invitrogen™)

The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or “click" reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne. The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min⁄Max format.

HCS DNA Damage Kit (Invitrogen™)

The HCS DNA Damage kit simultaneously and quantitatively measures two important cell-health parameters: DNA damage and cytotoxicity. DNA damage is detected using an antibody against phosphorylated H2AX (Ser139) which is induced in response to double-strand break (DSB) formation. To detect cytotoxicity, the Image-iT® DEAD™ Green is an impermeant dye in healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging (Invitrogen™)

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis.