Shop All Multiplex Luminex™ Protein Assays

Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Invitrogen™)

The Human Immune Monitoring 65-plex ProcartaPlex Panel for MagPix is a preconfigured multiplex immunoassay kit that measures 65 cytokines, chemokines, and growth factors using Luminex xMAP technology. Analyze 65 cytokines, chemokines, and growth factors targets on two 96-well plates compatible with the MagPix system for efficient immune response profiling, biomarker discovery, and validation. This kit enables efficient immune profiling, biomarker discovery, and validation on the MagPix instrument by providing two microplates (96-well) and reagents for 2 x 96 assays (due to the 50 analyte limitation of MagPix instruments). ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: Serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay
Target list: APRIL; BAFF; BLC; CD30; CD40L; ENA-78; Eotaxin; Eotaxin-2; Eotaxin-3; FGF-2; Fractalkine; G-CSF; GM-CSF; Gro a; HGF; IFN-a; IFN-g; IL-10; IL-12p70; IL-13; IL-15; IL-16; IL-17A; IL-18; IL-1a; IL-1b; IL-2; IL-20; IL-21; IL-22; IL-23; IL-27; IL-2R; IL-3; IL-31; IL-4; IL-5; IL-6; IL-7; IL-8; IL-9; IP-10; I-TAC; LIF; MCP-1; MCP-2; MCP-3; M-CSF; MDC; MIF; MIG; MIP1a; MIP-1b; MIP-3a; MMP-1; NGF beta; SCF; SDF-1a; TNF b; TNF-a; TNF-R2; TRAIL; TSLP; TWEAK; VEGF-A
Bead regions: See product documentation
Assay range: See product documentation
Assay sensitivity: See product documentation

Custom ProcartaPlex Multiplex Panel (Invitrogen™)

Design a custom ProcartaPlex immunoassay panel to assess multiple protein biomarkers in a single sample using Luminex™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.

Metabolism/Obesity 5-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Metabolism 5-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 5 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
Adiponectin; ICAM-1; NGAL; ZAG; RBP4

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Cytokine & Chemokine Convenience 36-Plex Mouse ProcartaPlex™ Panel 1A (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex Simplex or Multiplex Kits.

Reactivity/Species
Mouse

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-12p70; IL-13; IL-1 beta; IL-2; IL-4; IL-5; IL-6; TNF alpha; GM-CSF; IL-18; IL-10; IL-17A; IL-22; IL-23; IL-27; IL-9; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-1 beta; MIP-2; RANTES; Eotaxin; IFN alpha; IL-15/IL-15R; IL-28; IL-31; IL-1 alpha; IL-3; G-CSF; LIF; ENA-78/CXCL5; M-CSF

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Atherosclerosis 9-plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Atherosclerosis 9-plex ProcartaPlex Panel 1 is a preconfigured multiplex immunoassay kit that measures 9 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay
Target list: tenascin C, HSP 60, LOX-1, MPO, FGF-2, PAI-1, TRAIL, IL-8, IL-6
Bead regions: tenascin C: 28; HSP 60: 13; LOX-1: 76; MPO: 18; FGF-2: 75; PAI-1: 35; TRAIL: 66; IL-8: 27; IL-6: 25
Assay sensitivity (as determined for Lot 1): tenascin C: 119.2 pg/mL; HSP 60: 107.1 pg/mL; LOX-1: 0.3 pg/mL; MPO: 7.8 pg/mL; FGF-2: 4.5 pg/mL; PAI-1: 3.0 pg/mL; TRAIL: 0.6 pg/mL; IL-8: 0.2 pg/mL; IL-6: 1.5 pg/mL

Immuno-Oncology Checkpoint 14-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Immuno-Oncology Checkpoint 14-plex ProcartaPlex Panel 1 is a preconfigured multiplex immunoassay kit that measures 14 soluble forms of receptors and ligands for NK cells using Luminex xMAP technology. This panel can be combined with ProcartaPlex Human Immuno-Oncology Checkpoint Panel 2 to study a total of 28 cytokines, chemokines, and adhesion targets that play important roles in shaping local and systemic immune responses. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
BTLA; GITR; HVEM; IDO; LAG-3: 47; PD-1; PD-L1; PD-L2; TIM-3; CD28; CD80; CD137; CD27; CD152

Bead Regions
BTLA: 52; GITR: 57; HVEM: 36; IDO: 46; LAG-3; PD-1: 65; PD-L1: 66; PD-L2: 67; TIM-3: 14; CD28: 15; CD80: 61; CD137: 26; CD27: 27; CD152: 33

Assay Range
[As determined for Lot 1:] BTLA: 122.7-500000 pg/mL; GITR: 24.41-100000 pg/mL; HVEM: 24.41-100000 pg/mL; IDO: 4.88-20000 pg/mL; LAG-3: 12.21-50000 pg/mL; PD-1: 6.1-25000 pg/mL; PD-L1: 4.88-20000 pg/mL; PD-L2: 48.83-200000 pg/mL; TIM-3: 61.04-250000 pg/mL; CD28: 488.28-2000000 pg/mL; CD80: 30.52-125000 pg/mL; CD137: 12.21-50000 pg/mL; CD27: 4.88-20000 pg/mL; CD152: 12.21-50000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] BTLA: 38.21 pg/mL; GITR: 13.36 pg/mL; HVEM: 3.49 pg/mL; IDO: 4.2 pg/mL; LAG-3: 3.37 pg/mL; PD-1: 5.04 pg/mL; PD-L1: 1.96 pg/mL; PD-L2: 48.17 pg/mL; TIM-3: 22.66 pg/mL; CD28: 437 pg/mL; CD80: 14.33 pg/mL; CD137: 7.87 pg/mL; CD27: 1.72 pg/mL; CD152: 4.72 pg/mL

More Product Data
ProcartaPlex Human Immuno-Oncology Checkpoint Panel: Detection of soluble isoforms of immuno-oncology checkpoint markers

TIM-3 Mouse ProcartaPlex™ Simplex Kit (Invitrogen™)

The Mouse TIM-3 Simplex ProcartaPlex kit measures TIM-3 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: mouse
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

Coagulation 3-Plex Human ProcartaPlex™ Panel 2 (Invitrogen™)

The Human Coagulation II 3-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 3 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
Plasma (Citrat, EDTA)

Sample Volume
Plasma (Citrat, EDTA): 25 µL

Reported Application
Multiplex Immunoassay

Target List
Factor V; Factor VII; Factor VIII

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Apolipoprotein 5-plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human Apolipoprotein 5-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 5 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: serum, plasma
Sample volume: 25 µL (serum, plasma)
Reported application: multiplex immunoassay
Target list: apolipoprotein A1, apolipoprotein A2, apolipoprotein B, apolipoprotein C2, apolipoprotein E
Bead regions: APO A1: 57; APO A2: 55; APO B: 38; APO C2: 56; APO E: 77
Assay range (as determined for Lot 1): APO A1: 0.73-3000 ng/mL; APO A2: 0.02-100 ng/mL; APO B: 4.88-20000 ng/mL; APO C2: 0.56-2300 ng/mL; APO E: 0.22-900 ng/mL
Assay sensitivity (as determined for Lot 1): APO A1: 0.179 ng/mL; APO A2: 0.003 ng/mL; APO B: 4.880 ng/mL; APO C2: 0.373 ng/mL; APO E: 0.061 ng/mL

Cytokine/Chemokine/Growth Factor Convenience 45-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configurated panels and combinable single analytes.

NOTE: This kit cannot be combined with other ProcartaPlex Simplex or Multiplex Kits.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
BDNF; Eotaxin/CCL11; EGF; FGF-2; GM-CSF; GRO alpha/CXCL1; HGF; NGF beta; LIF; IFN alpha; IFN gamma; IL-1 beta; IL-1 alpha; IL-1RA; IL-2; IL-4; IL-5; IL-6; IL-7; IL-8/CXCL8; IL-9; IL-10; IL-12 p70; IL-13; IL-15; IL-17A; IL-18; IL-21; IL-22; IL-23; IL-27; IL-31; IP-10/CXCL10; MCP-1/CCL2; MIP-1 alpha/CCL3; MIP-1 beta/CCL4; RANTES/CCL5; SDF-1 alpha/CXCL12; TNF alpha; TNF beta/LTA; PDGF-BB; PLGF; SCF; VEGF-A; VEGF-D

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Chemokine 8-Plex Rat ProcartaPlex™ Panel (Invitrogen™)

The Rat Chemokine 8-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 8 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
Eotaxin; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-2; Rantes

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] Eotaxin: 1.9-7700 pg/mL; GRO alpha: 11.4-46500 pg/mL; IP-10: 4.9-20100 pg/mL; MCP-1: 18.03-75000 pg/mL; MCP-3: 3.5-14200 pg/mL; MIP-1 alpha: 2.3-9500 pg/mL; MIP-2: 0.6-2400 pg/mL; Rantes: 85.5-350000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] Eotaxin: 1.1 pg/mL; GRO alpha: 1.4 pg/mL; IP-10: 0.9 pg/mL; MCP-1: 10.9 pg/mL; MCP-3: 1.1 pg/mL; MIP-1 alpha: 1.0 pg/mL; MIP-2: 0.1 pg/mL; Rantes: 6.1 pg/mL

Th1/Th2/Th9/Th17 Cytokine 18-Plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human Th1/Th2/Th9/TH17 Cytokine 18-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 18 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
GM-CSF; IFN gamma; IL-1 beta; IL-2; IL-4; IL-5; IL-6; IL-9; IL-10; IL-12p70; IL-13; IL-17A; IL-18; IL-21; IL-22; IL-23; IL-27; TNF alpha

Bead Regions
IFN gamma: 43; IL-12p70: 34; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF alpha: 45; GM-CSF: 44; IL-18: 66; IL-10: 28; IL-17A: 36; IL-21: 72; IL-22: 76; IL-23: 63; IL-27: 14; IL-9: 52

Assay Range
[As determined for Lot 1:] IFN gamma: 12.21-50000 pg/mL; IL-12p70: 6.84-28000 pg/mL; IL-13: 2.44-10000 pg/mL; IL-1 beta: 2.44-10000 pg/mL; IL-2: 4.88-20000 pg/mL; IL-4: 12.21-50000 pg/mL; IL-5: 7.32-30000 pg/mL; IL-6: 9.77-40000 pg/mL; TNF alpha: 8.54-35000 pg/mL; GM-SCF: 17.09-70000 pg/mL; IL-18: 9.77-40000 pg/mL; IL-10: 2.44-10000 pg/mL; IL 17A: 2.44-10000 pg/mL; IL-21: 9.77-40000 pg/mL; IL-22: 31.74-130000 pg/mL; IL-23: 14.65-60000 pg/mL; IL-27: 24.41-100000 pg/mL; IL-9: 9.77-40000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IFN gamma: 0.2 pg/mL; IL-12p70: 0.04 pg/mL; IL-13: 0.1 pg/ml; IL-1 beta: 0.2 pg/mL; IL-2: 0.8 pg/mL; IL-4: 1.5 pg/mL; IL-5: 0.3 pg/mL; IL-6: 0.4 pg/mL; TNF alpha: 0.4 pg/mL; GM-CSF: 1.2 pg/mL; IL-18 : 0.4 pg/mL; IL-10: 0.1 pg/mL; IL-17A: 0.1 pg/mL; IL-21: 0.6 pg/mL; IL-22: 8.2 pg/mL; IL-23: 0.9 pg/mL; IL-27: 5.1 pg/mL; IL-9: 0.5 pg/mL

Th1/Th2 Cytokine & Chemokine 20-Plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Th1/Th2 Cytokine & Chemokine 20-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 20 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Human

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
GM-CSF; IFN gamma; IL-1 beta; IL-2; IL-4; IL-5; IL-6; IL-12 p70; IL-13; IL-18; TNF alpha; Eotaxin; GRO-alpha; IL-8; IP-10; MCP-1; MIP-1 alpha; MIP-1 beta; Rantes; SDF-1 alpha

Bead Regions
IFN-gamma: 43; IL-12p70: 34; IL-13: 35; IL-1 beta: 18; IL-2: 19; IL-4: 20; IL-5: 21; IL-6: 25; TNF-alpha: 45; GM-CSF: 44; IL-18: 66; Eotaxin: 33; GRO-alpha: 61; IL-8: 27; IP-10: 22; MCP-1: 51; MIP-1 alpha: 12; MIP-1 beta: 47; Rantes: 42; SDF-1 alpha: 13

Assay Range
[As determined for Lot 1:] IFN gamma: 12.21-50000 pg/mL; IL-12p70: 6.84-28000 pg/mL; IL-13: 2.44-10000 pg/mL; IL-1 beta: 2.44-10000 pg/mL; IL-2: 4.88-20000 pg/mL; IL-4: 12.21-50000 pg/mL; IL-5: 7.32-30000 pg/mL; IL-6: 9.77-40000 pg/mL; TNF-alpha: 8.54-35000 pg/mL; GM-SCF: 17.09-70000; IL-18: 9.77-40000 pg/mLAs; Eotaxin: 0.61-2500 pg/mL; GRO-alpha: 2.44-10000 pg/mL; IL-8: 2.44-10000 pg/mL; IP-10: 1.95-8000 pg/mL; MCP-1: 1.22-5000 pg/mL; MIP-1 alpha: 2.14-8750 pg/mL; MIP-1 beta: 6.10-25000 pg/mL; Rantes: 0.61-2500 pg/mL; SDF1-alpha: 17.09-70000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IFN gamma: 0.2 pg/mL; IL-12p70: 0.04 pg/mL; IL-13: 0.1 pg/ml; IL-1 beta: 0.2 pg/mL; IL-2: 0.8 pg/mL; IL-4: 1.5 pg/mL; IL-5: 0.3 pg/mL; IL-6: 0.4 pg/mL; TNF-alpha: 0.4 pg/mL; GM-CSF: 1.2 pg/mL; IL-18: 0.4 pg/mL; Eotaxin: 1.4 pg/mL; GRO-alpha: 2.8 pg/mL; IL-8: 1.2 pg/mL; IP-10: 0.3 pg/mL; MCP-1: 0.6 pg/mL; MIP-1 alpha: 1.1 pg/mL; MIP-1 beta: 4.7 pg/mL; Rantes: 0.2 pg/mL; SDF1-alpha: 20.5 pg/mL

Brain Injury 4-plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human Brain Injury 4-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 4 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: serum, plasma, CSF
Sample volume: 25 µL (serum, plasma, CSF)
Reported application: multiplex immunoassay
Target list: GFAP, neurofilament heavy (NF-H), UCHL1, S100B
Bead regions: GFAP: 22, neurofilament heavy (NF-H): 33, UCHL1: 19, S100B: 18

Cytokine & Chemokine 22-Plex Rat ProcartaPlex™ Panel (Invitrogen™)

The Rat Cytokine & Chemokine 22-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 22 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Rat

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IL-1 alpha; G-CSF; IL-10; IL-17A; IL-1 beta; IL-6; TNF alpha; IL-4; GM-CSF; IFN gamma; IL-2; IL-5; IL-13; IL-12p70; Eotaxin; GRO alpha; IP-10; MCP-1; MCP-3; MIP-1 alpha; MIP-2; Rantes

Bead Regions
See product documentation

Assay Range
[As determined for Lot 1:] IL-1 alpha: 12.2-50000 pg/mL; C-CSF (CSF-3): 3.7-15000 pg/mL; IL-10: 9.8-40000 pg/mL; IL-17A: 3.7-15000 pg/mL; IL-1 beta: 15.9-65000 pg/mL; IL-6: 4.9-20000 pg/mL; TNF alpha: 4.9-20000 pg/mL; IL-4: 1.2-5000 pg/mL; GM-CSF: 9.8-40000 pg/mL; IFN gamma: 9.8-40000 pg/mL; IL-2: 4.9-20000 pg/mL; IL-5: 2.4-10000 pg/mL; IL-13: 4.9-20000 pg/mL; IL-12p70: 9.8-40000 pg/mL; Eotaxin: 1.9-7700 pg/mL; GRO alpha: 11.4-46500 pg/mL; IP-10: 4.9-20100 pg/mL; MCP-1: 18.03-75000 pg/mL; MCP-3: 3.5-14200 pg/mL; MIP-1 alpha: 2.3-9500 pg/mL; MIP-2: 0.6-2400 pg/mL; Rantes: 85.5-350000 pg/mL

Assay Sensitivity
[As determined for Lot 1:] IL-1 alpha: 3.5 pg/mL; C-CSF (CSF-3): 0.3 pg/mL; IL-10: 1.6 pg/mL; IL-17A: 0.2 pg/mL; IL-1 beta: 1.1 pg/mL; IL-6: 1.9 pg/mL; TNF alpha: 0.4 pg/mL; IL-4: 0.1 pg/mL; GM-CSF: 1.3 pg/mL; IFN gamma: 0.6 pg/mL; IL-2: 1.9 pg/mL; IL-5: 0.3 pg/mL; IL-13: 0.3 pg/mL; IL-12p70: 5.9 pg/mL; Eotaxin: 1.1 pg/mL; GRO alpha: 1.4 pg/mL; IP-10: 0.9 pg/mL; MCP-1: 10.9 pg/mL; MCP-3: 1.1 pg/mL; MIP-1 alpha: 1.0 pg/mL; MIP-2: 0.1 pg/mL; Rantes: 6.1 pg/mL