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Pierce™ Fast Western Blot Kit, ECL Substrate (Thermo Scientific™)

The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Features of this kit:

Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $8 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.

SuperSignal™ West Pico Complete Mouse IgG Detection Kit (Thermo Scientific™)

Thermo Scientific SuperSignal West Pico Complete Mouse IgG Detection Kit is a a kit containing goat anti-mouse secondary antibody HRP conjugate for the detection of mouse primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Mouse IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit

SuperSignal™ West Pico Mouse IgG Detection Kit (Thermo Scientific™)

Thermo Scientific SuperSignal West Pico Complete Mouse IgG Detection Kit is a a kit containing goat anti-mouse secondary antibody HRP conjugate for the detection of mouse primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Mouse IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit

Clean-Blot™ IP Detection Reagent (HRP) (Thermo Scientific™)

Thermo Scientific Clean-Blot IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and Kit eliminate detection-interference from both heavy-chain (approx. 50kDa) and light-chain (25kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.

Features of the Clean-Blot IP Detection Reagent:

Universal—bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
Compatible—effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
Cost-effective—eliminates the cost and extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
Flexible—horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
Easy to use—no need to change the Western blotting protocol; simply replace conventional secondary HRP conjugate with the Clean-Blot IP Detection Reagent
Unobstructed detection—clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands

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Clean-Blot™ IP Detection Kit (HRP)

SuperSignal™ West Pico Rabbit IgG Detection Kit (Thermo Scientific™)

Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP conjugate for the detection of rabbit primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Rabbit IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit

iBind™ Fluorescent Detection (FD) Solution Kit (Invitrogen™)

The iBind Fluorescent Detection (FD) Solution Kit is intended for use with the iBind Western Device and is optimized for western blotting with fluorescent-conjugated antibodies, including those commonly used for infrared detection and dual wavelength semi-quantitative analysis. The iBind Fluorescent Detection Solution Kit is optimized with the ideal viscosity for blocking, washing, and antibody dilution.

Learn more about the iBind western systems ›

The iBind Fluorescent Detection (FD) Solution Kit, together with the iBind Western Device and iBind Cards, bring automation and simplicity to your western protocol. Each iBind Fluorescent Detection (FD) Solution Kit contains sufficient solution for 10 mini-membrane reactions. An iBind Western Device and iBind Cards are required for use of this kit.

About the iBind Western Device
The iBind Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source. Each solution is released from the iBind Device wells to an iBind Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Novex™ AP Mouse Chemiluminescent Detection Kit (Invitrogen™)

The Novex® AP Mouse Chemiluminescent Detection Kit provides superior chemiluminescent detection of proteins transferred to either nitrocellulose or PVDF membranes and treated with a protein-specific primary antibody. Detection is performed by treatment of the membrane with an alkaline phosphatase (AP)-conjugated anti-mouse secondary antibody followed by a ready-to-use chemiluminescent CDP-Star® substrate of alkaline phosphatase. Protein-specific signal is then captured by a chemiluminescent-compatible imaging system or X-ray film.

The Novex® AP Mouse Chemiluminescent Detection Kit is compatible with most western protocols but was designed for use with the iBind™ Western System.

Each kit includes a secondary AP-conjugated anti-mouse antibody, CDP-Star® chemiluminescent substrate, and an enhancer, sufficient for 10 mini-membranes processed using the iBind™ Western System.

Features include:

• Easy-to-use protocol
• High specificity, clean background
• Ultra-sensitivity
• Long-lasting signals—up to 5 days
• Results in less 5 minutes

Pierce™ Fast Western Kit, SuperSignal™ West Pico, Mouse (Thermo Scientific™)

Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—picogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $14 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate.The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.

The Pierce Fast Western Blot Kits are reagent-based system that provide optimized reagents for blocking, antibody dilution and detection of Western blots. The Fast Western Blot Kit protocol is quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other popular rapid Western blotting systems.

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Pierce™ Fast Western Kit, SuperSignal™ West Pico, Rabbit

Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Rabbit (Thermo Scientific™)

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Easy—complete kits contain all components needed to block, probe and develop a blot with your rabbit primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate's sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with a rabbit primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Mouse

SuperSignal™ West Pico Complete Rabbit IgG Detection Kit (Thermo Scientific™)

Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP conjugate for the detection of rabbit primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Rabbit IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit

WesternBreeze™ Chromogenic Kit, anti-goat (Invitrogen™)

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase. Non-fading purple precipitates develop at the protein bands on the membrane. WesternBreeze® chromogenic offers:

• Clear background
• High sensitivity-low picogram levels detectable
• High specificity
• One simple protocol-no optimization required

Clean-Blot™ IP Detection Kit (HRP) (Thermo Scientific™)

Thermo Scientific Clean-Blot IP Detection Kit (HRP) contains enough Clean-Blot IP Detection Reagent and accessory reagents for 25 mini-blots or 2000 cm2 of membrane. Clean-Blot IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and Kit eliminate detection-interference from both heavy-chain (approx. 50kDa) and light-chain (25kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.

Features of the Clean-Blot IP Detection Reagent:

Universal—bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
Compatible—effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
Cost-effective—eliminates the cost and extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
Flexible—horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
Easy to use—no need to change the Western blotting protocol; simply replace conventional secondary HRP conjugate with the Clean-Blot IP Detection Reagent
Unobstructed detection—clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands

Included in this kit:
Kit contains Clean-Blot Detection Reagent (HRP), blocking buffer and Thermo Scientific Pierce ECL Substrate (chemiluminescent)

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Clean-Blot™ IP Detection Reagent (HRP)

Pierce™ Far-Western Blot Kit for Biotinylated Proteins (Thermo Scientific™)

The Thermo Scientific Pierce Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins via interaction with any biotin-labeled test protein used as the primary probe.

Features of the Far-Western Blot Kit for Biotinylated Proteins:

On-membrane or in-gel detection options—on-membrane detection offers greater sensitivity; in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer
Nonradioactive alternative for far-Western analysis—reliable and sensitive biotin/streptavidin-HRP chemistry combined with chemiluminescent detection offers a practical and safe alternative to radiolabeling the bait protein
Useful interaction range—effective for interaction pairs that have moderate to strong binding associations between the prey and the biotinylated bait protein
Primary antibody-free detection—uses a biotinylated protein as the probe, eliminating the need for primary antibodies or even to have prior knowledge of target-protein identities
Compatible with both SDS-PAGE and native gels—provides option to probe for prey proteins in a more native environment because reduced or denaturing systems may not always allow for the intended interaction
Reduced nonspecific binding—biotin/streptavidin-HRP systems demonstrate less nonspecific binding compared to antibodies directed against the bait protein
Compatible with protein staining—gels can be used for total protein staining after the in-gel chemiluminescent detection step, eliminating the need to run two gels

This far-Western blotting kit provides the reagents and protocol necessary to probe for target proteins by virtue of their interaction with a specific binding partner, which the researcher supplies in purified, biotinylated form. The kit is successful for analysis of protein-protein interactions in which the target (prey) proteins can be bound after they have been separated by gel electrophoresis. The kit includes reagents and procedures for probing and chemiluminescent detection with blots or directly from mini gels.

Includes:
• Streptavidin-HRP conjugate, dilution and wash buffers, chemiluminescent substrate solutions, and cellophane sheets for mini gels

Applications:
• Protein interaction discovery—search and screen for proteins in cell lysates that have binding affinity to the purified biotinylated protein
• Protein interaction confirmation—characterize and identify the presence of native or off-target binding interactions involving a given biotinylated protein and particular samples

Far-Western blotting involves probing for targets first with a putative interaction-protein rather than a primary antibody. The method is called far-Western blotting because primary antibodies are used secondarily to detect the primary interaction probe rather than the target protein. The Pierce Far-Western Blot Kit for Biotinylated Proteins eliminates the use of antibodies altogether. The primary probe (i.e., the biotinylated bait protein supplied by the researcher) is secondarily detected with streptavidin-HRP conjugate, whose activity is visualized by reaction with a very sensitive chemiluminescent substrate.

Prionics™-Check WESTERN Kit (12000)

The Prionics®-Check WESTERN Kit is the gold standard of rapid BSE testing and is used as a confirmatory test by most National Reference Laboratories across Europe. It owes its success to its precision and reliability. The test uses the relative protease resistance of the disease-specific prion protein PrPSc for TSE detection. Protease digestion leads to a small decrease in PrPSc protein size, whereas the normal protein PrPC is completely degraded.

The Prionics®-Check WESTERN Kit delivers a completely reliable test-result based on three independent and easily interpretable criteria: signal (PrPSc), characteristic three-band pattern BSE positive, position relative to control (PrPC).

Test procedure
1. The homogenized brain sample is incubated with an optimized reagent mixture consisting of digestion enzymes and a buffer solution. This solution degrades the normal prion protein. Only the BSE-specific prion protein remains in the test sample.
2. The proteins in the sample are then separated according to size by gel electrophoresis and transferred to a special blot membrane for detection. The BSE-specific prion protein on the membrane is detected with specific antibodies and is visualized on a digital file or on a film.

iBind™ Solution Kit (Invitrogen™)

The iBind Solutions Kit is intended for use with the iBind Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. The iBind solutions act as the blocking, washing, and dilution solutions in the iBind immunodetection process and have been optimized for ideal viscosity. Each solution is released from the iBind device wells to the iBind Card through sequential lateral flow (SLF); the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Learn more about the iBind western systems ›

The iBind Solutions Kit, together with the iBind Western Device and iBind Cards, bring automation and simplicity to the western protocol. Each iBind Solutions Kit contains sufficient solution for 10 mini-membrane reactions. An iBind Western Device is required for use of this kit. For fluorescent applications use the iBind Fluorescent Detection (FD) Solution Kit.