Shop All Western Blotting Kits

WesternBreeze™ Chemiluminescent Kit, anti-goat (Invitrogen™)

WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:

• High specificity, clean background
• Ultra-sensitivity-femtogram levels detectable
• Long-lasting signals-up to 5 days
• Results in less than 3 hours
• Permanent photographic image

Pierce™ Fast Western Blot Kit, ECL Substrate (Thermo Scientific™)

The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Features of this kit:

Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $8 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.

SuperSignal™ West Pico Complete Mouse IgG Detection Kit (Thermo Scientific™)

Thermo Scientific SuperSignal West Pico Complete Mouse IgG Detection Kit is a a kit containing goat anti-mouse secondary antibody HRP conjugate for the detection of mouse primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Mouse IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit

Pierce™ Background Eliminator Kit (Thermo Scientific™)

The Thermo Scientific Pierce Background Eliminator Kit provides fast, easy removal of haze and artifacts on developed X-ray film to correct for overexposure of blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography.

Features of the Background Eliminator Kit:

For overexposed X-ray film—reverses development of silver-based autoradiography film removing silver emulsion from the film surface
Recovers and preserved data—evenly reduces developed signal over the entire film so relative intensity (densitometry) of bands is not altered
Optimizes for signal-to-noise—allows readjustment of overdeveloped film to obtain the best image with respect to background haze, speckles and oversaturated bands that need to be resolved
Saves time and resources—eliminates the need to repeat experiments or re-expose a new piece of film to obtain an optimal image

This two-reagent kit is simple to use as demonstrated in the video below. Mix the two reagents (1:1 ratio) and immerse a developed piece of film (recent or old) in the working solution. Watch for 1 to 5 minutes until the desired image appears, then stop the reaction by rinsing the film in water. Pierce Background Eliminator for Film evenly reverses development-signal over the entire film, preserving relative densitometry values so that analysis and publication of results is still valid and possible.

Applications:
• X-ray film developed from enhanced chemiluminescence Western blots of any kind, whether from Thermo Scientific SuperSignal, Pierce ECL or other commercial substrate
• X-ray film developed from any chemiluminescent or radioactive gel-shift assays, including Thermo Scientific LightShift Chemiluminescent EMSA Kits for DNA or RNA
• Autoradiography film developed from chemiluminescent or isotopic (32P or 35S) Northern or Southern blots, including with Thermo Scientific North2South Chemiluminescent Detection Kit
• Any developed silver-emulsion photographic film, whether blue, clear, single- or double-sided, and regardless of the method of exposure

High background, shading, overexposed bands and speckling are problems inherent to film exposure. High background and shading are caused by overexposure, poor use of blocking buffer or inappropriate enzyme-labeled probe or antibody concentration. Overexposed bands are a common occurrence when the enzyme-labeled probe or antibody used is too concentrated or if the film was exposed for too long. Speckling and shading occur when enzyme conjugates form complexes and precipitate on the blot. Pierce Background Eliminator can correct all these problems without the need to re-expose a blot to film or re-do an entire experiment. The working solution can be used with newly exposed films or those that have been stored for years. In addition, the product can be used with any brand of film.

Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Mouse (Thermo Scientific™)

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate's sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with a mouse primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Rabbit

Novex™ AP Rabbit Chemiluminescent Detection Kit (Invitrogen™)

The Novex® AP Rabbit Chemiluminescent Detection Kit provides superior chemiluminescent detection of proteins transferred to either nitrocellulose or PVDF membranes and treated with a protein-specific primary antibody. Detection is performed by treatment of the membrane with an alkaline phosphatase (AP)-conjugated anti-rabbit secondary antibody followed by a ready-to-use chemiluminescent CDP-Star® substrate of alkaline phosphatase. Protein-specific signal is then captured by a chemiluminescent-compatible imaging system or X-ray film.

The Novex® AP Rabbit Chemiluminescent Detection Kit is compatible with most western protocols but was designed for use with the iBind™ Western System.

Each kit includes a secondary AP-conjugated anti-rabbit antibody, CDP-Star® chemiluminescent substrate, and an enhancer, sufficient for 10 mini-membranes processed using the iBind™ Western System.

Features include:

• Easy to use protocol
• High specificity, clean background
• Ultra-sensitivity
• Long-lasting signals--up to 5 days
• Results in less 5 minutes

HENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit (Thermo Scientific™)

HENS Buffer consists of 100 mM HEPES (pH 7.8), 1 mM EDTA, 0.1 mM Neocuproine, and 1% SDS. It is a cell lysis buffer intended for use with the Thermo Scientific Pierce S-Nitrosylation Western Blot Kit, which enables sensitive detection of protein S-nitrosocysteine post-translational modifications in a complete, easy-to-use kit.

More Product Data
Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents

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Pierce™ S-Nitrosylation Western Blot Kit

WesternBreeze™ Chemiluminescent Kit, anti-rabbit (Invitrogen™)

WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:

• High specificity, clean background
• Ultra-sensitivity-femtogram levels detectable
• Long-lasting signals-up to 5 days
• Results in less than 3 hours
• Permanent photographic image

Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Mouse (Thermo Scientific™)

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid, 1-hour chemiluminescent Western blotting at femtogram levels of sensitivity.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Rabbit

iBind™ Solution Kit (Invitrogen™)

The iBind Solutions Kit is intended for use with the iBind Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. The iBind solutions act as the blocking, washing, and dilution solutions in the iBind immunodetection process and have been optimized for ideal viscosity. Each solution is released from the iBind device wells to the iBind Card through sequential lateral flow (SLF); the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Features include:
• Optimized solutions with ideal viscosity for blocking, washing, and antibody dilution
• Long shelf life—store iBind Solutions Kit for 12 months at 4°C
• Better antigen-antibody interaction—solutions flow between the iBind Card and membrane for maximum interaction between protein and antibody

The iBind Solutions Kit, together with the iBind Western Device and iBind Cards, bring automation and simplicity to the western protocol. Each iBind Solutions Kit contains sufficient solution for 10 mini-membrane reactions. An iBind Western Device is required for use of this kit.

Learn more about the iBind western systems ›

SuperSignal™ West Pico Mouse IgG Detection Kit (Thermo Scientific™)

Thermo Scientific SuperSignal West Pico Complete Mouse IgG Detection Kit is a a kit containing goat anti-mouse secondary antibody HRP conjugate for the detection of mouse primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Mouse IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit

SuperSignal™ West Pico Complete Rabbit IgG Detection Kit (Thermo Scientific™)

Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP conjugate for the detection of rabbit primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Rabbit IgG Detection Kit:

Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

Related Products
SuperSignal™ West Pico Complete Mouse IgG Detection Kit
SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Rabbit IgG Detection Kit

HisProbe™-HRP Conjugate (Thermo Scientific™)

Thermo Scientific HisProbe-HRP is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

Features of HisProbe-HRP Conjugate:

Fast—His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
Economical—no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
High specificity—HisProbe-HRP can recognize his-tags independent of adjacent tags.
Less steric hindrance—HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.

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SuperSignal™ West HisProbe Kit

Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Rabbit (Thermo Scientific™)

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Easy—complete kits contain all components needed to block, probe and develop a blot with your rabbit primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate's sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with a rabbit primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Mouse

WesternBreeze™ Chromogenic Kit, anti-mouse (Invitrogen™)

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase. Non-fading purple precipitates develop at the protein bands on the membrane. WesternBreeze® chromogenic offers:

• Clear background
• High sensitivity-low picogram levels detectable
• High specificity
• One simple protocol-no optimization required