Shop All DNA⁄RNA Detection & Analysis Kits

100X Pretreatment Solution Invitrogen™

100X Pretreatment Solution is an aqueous buffered solution and an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays.

Wash Buffer Components (1 and 2) Invitrogen™

Wash Buffer Components (1 and 2) is an individual component of the ViewRNA™ ISH Tissue Assay Kits, 1-Plex, QVT0050. It is an aqueous buffered solution containing detergent, and can be used for 24 assays (108 mL). Store at -15°C to -30°C.

FISH Tag™ RNA Multicolor Kit, Alexa Fluor™ dye combination Invitrogen™

The FISH Tag™ RNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ RNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for the simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts. We also offer FISH Tag™ Kits in single colors.

FISH Tag™ Kit Specifications:

• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm), Alexa Fluor® 555 (555/565 nm), Alexa Fluor® 594 (590/615 nm), Alexa Fluor® 647 (650/670 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

North2South™ Chemiluminescent Hybridization and Detection Kit Thermo Scientific™

Thermo Scientific North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

Features of the North2South Chemiluminescent Detection Kit:

• Sensitivity equal to or greater than 32P
• Much faster than DIG detection systems
• Amenable to nearly any DNA or RNA hybridization method
• Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
• High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
• Specific plaques can be picked out of areas that are very dense with signal due to extremely low background

The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Related Products
North2South™ Complete Biotin Random Prime Labeling and Detection Kit
North2South™ Chemiluminescent Substrate for HRP
North2South™ Hybridization Buffer
North2South™ Hybridization Stringency Wash Buffer (2X)

MethylMiner™ Methylated DNA Enrichment Kit Applied Biosystems™

The MethylMiner™ Methylated DNA Enrichment Kit is for highly sensitive enrichment of methylated DNA for downstream analysis including PCR/qPCR based assays, bisuflite conversion followed by amplification, cloning, and sequencing, direct sequencing, library preparation for high-throughput sequencing, and sample-prep for DNA microarray analysis. Patterns of DNA methylation seem to be important for making determinations about development and diseases such as cancer. This kit enables superior enrichment and differential fractionation of double-stranded DNA based on CpG methylation density, with increased sensitivity over antibody-based methods. Fractionation permits important comparisons between samples and enables researchers to focus analysis on only the methylation densities of interest.
• Partitioning with high affinity binding—at least 4-fold greater sensitivity than antibody-based methods
• Fractionation based on CpG methylation density—ds DNA capture is achieved with MBD2 protein and facilitates ligation of double-stranded adaptors for next-generation sequencing
• Rapid and easy elution with salt eliminates the need for proteinase K treatment and phenol:chloroform extraction
• Precise answers—fractionated DNA permits distinctions to be made regarding methylation status and density
• Fast protocol—completed in less than 4 hours
• Easy—simple handling with Dynabeads® the gold standard magnetic beads

ViewRNA™ Fast Red Buffer and Substrate Kit Invitrogen™

ViewRNA Fast Red Buffer and Substrate Kit is an individual component of the ViewRNA Chromogenic Signal Amplification Kit, 24 assays. It is a red substrate used for the detection of alkaline phosphatase activity.

TaqMan™ GMO Maize Quantification Kit

The Taqman® GMO Maize Quantification Kit is a food safety testing kit used to determine the number of copies of P35S promoter present in genetically modified organisms compared to the number of copies of maize present in any food or feed sample. The benefits of the Taqman® GMO Maize Quantification Kit over other commercially available products include:

• Efficiency—detection of small DNA fragments through use of Taqman® MGB probes
• Sensitivity—quantification limit of 20 DNA copies; detection limit of 3 DNA copies (maize and P355 systems)
• Flexibility—adapted to processed DNAs

The Taqman® GMO Maize Quantification Kit is effective in detecting the presence of genetically modified organisms through transgenic maize quantification, based on the detection of P35S promoter. The detection success of this promoter is due to this regulatory region being present in most of the transgenic events. In addition, the Taqman® GMO Maize Quantification Kit contains all necessary reagents for quantification and is optimized for use in all (Applied Biosystems®) real-time PCR instruments.

The development and manufacture of this kit occurs through a partnership with IMEGEN (Instituto de Medicina Genomica), which has a long history of GMO research and development.

Other related products include:
GMO Extraction Kit
TaqMan® GMO Screening Kit
Taqman® Roundup Ready Soya Quantification Kit

FISH Tag™ DNA Green Kit, with Alexa Fluor™ 488 dye Invitrogen™

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ViewRNA™ Tissue Assay Blue Module Invitrogen™

This VewRNA Tissue Assay Blue Module is designed for use with the ViewRNA Tissue Assay Core Kit. The Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).

Learn more about and order ViewRNA Tissue probe sets ›

ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.

With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets

The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue or OCT (optimal cutting temperature) embedded frozen tissue sections. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.

MEGAshortscript™ T7 Transcription Kit Invitrogen™

The MEGAshortscript™ T7 Kit is designed for high yields of in vitro- transcribed RNA products, in the 20–500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.

Advantages of the MEGAshortscript™ Kit:

• Efficiently transcribes small templates (<300 bases) with consistently high yields
• Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates

Note:The MEGAshortscript™ T7 Kit is optimized for use with the T7 polymerase included in the kit. Using a different polymerase may result in low yields.

MAXIscript™ SP6 Transcription Kit Invitrogen™

The Ambion® MAXIscript® SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 100 reactions.

Features:

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript® In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.

FISH Tag™ DNA Orange Kit, with Alexa Fluor™ 555 dye Invitrogen™

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 555 (555/565 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

TaqMan™ GMO Soy 35S Detection Kit, with protocol Applied Biosystems™

This kit contains reagents needed to reliably detect the presence of genetically modified organism (GMO)-specific DNA sequences in seed, grain, processed foods and their ingredients.
• Universal assay detects and quantifies all approved genetically modified organisms (GMOs) in Europe and the vast majority of GMOs approved in other countries.
• Fluorogenic 5' nuclease assay using TaqMan® probes provides accurate and reproducible quantitative results.
• Label license conveys the PCR service rights for GMO testing when used in conjunction with an Authorized Thermal Cycler, so no additional royalties are payable.
• Comprehensive controls provide high confidence in results.
• Innovative GMO quantitation software allows users to quickly and easily determine the percentage of GMO content.
• Offers the complete solution including sample preparation, instruments, reagents, analysis software, technical support, instrument service, and GMO expertise.

The Complete Solution
The TaqMan® GMO Detection and Quantitation Kit is the first of a full product line of GMO assays in development that can detect the presence of GMO-specific DNA sequences in seed, grain, and processed foods and their ingredients. The TaqMan® GMO kit is part of a highly sophisticated but user-friendly system that includes DNA sample preparation, automated PCR amplification and signal detection, data analysis, and quantitation software, thus eliminating the need for personnel trained in molecular techniques.

Reliable TaqMan® Chemistry
The system uses the patented fluorogenic 5' nuclease method for multiplex detection and quantitation, the latest innovation in real-time PCR technology. It targets the Cauliflower Mosaic Virus 35S promoter, a GMO-specific sequence present in all GMO soy and maize events approved for use in food by the European Union, and in the vast majority of GMO events approved by other countries. Both the European Union and Japan view this method as the premier technology for quantitating GMO content in food. Other TaqMan® kits are available for the detection and quantitation of food-borne pathogens, including Salmonella and E. coli 0157:H7.

For Research Use Only. Not for use in diagnostics procedures.

ViewRNA™ ISH Cell Assay Kit Invitrogen™

The ViewRNA™ ISH Cell Assay is a direct fluorescence RNA in situ hybridization method that enables the simultaneous detection of one to four RNA targets (either mRNA or non-coding RNA) at single copy sensitivity and single cell resolution using fluorescence microscopes or high-content imagers. Unlike traditional FISH techniques which are generally limited by high background and low sensitivity, the assay uses proprietary chemistry for the target specific probe sets (RNA FISH probes) and branch DNA signal amplification (bDNA) for detection of specific signal. The fluorescence RNA in situ hybridization assay has four main steps: sample preparation, target hybridization, signal amplification, and detection. This ViewRNA ISH assay kit can detect up to three RNA but can be combined with the ViewRNA™ 740 Module to add the fourth probe.

Required additional components:
10X PBS (cat. no. QVC0508)
ViewRNA™ - Detergent Solution QC (cat. No. QVC0509)
ViewRNA™ - Wash Buffer Set (cat. no. QG0507)
ViewRNA™ ISH Cell Accessory Kit (cat. no. QVC0700) intended to provide many of the required components, not supplied in the reagent kit, in order to perform the assay.
ViewRNA™ Probe Sets are not included in the assay and are designed for use with the ViewRNA ISH Cell Assays. Visit our website to view a complete listing of over 6,500 synthesized Probe Sets. By request, new Probe Sets can be designed and synthesized in less than two weeks with no additional costs.

To add the 4th RNA probe:
ViewRNA™ ISH Cell 740 Module (cat. no. QVC0200) designed to be used in conjunction with ViewRNA™ ISH Cell Assay and allows analysis of an additional RNA target in the 740 channel (AlexaFluor 750) See the package insert for a complete list of materials provided in the kit.

Reported Applications
Microscopy

MAGnify™ Chromatin Immunoprecipitation System Applied Biosystems™

The MAGnify™ Chromatin Immunoprecipitation System provides a streamlined, optimized assay for the enrichment of chromatin/protein complexes and DNA recovery using magnetic bead capture technology. The isolated DNA is ready for downstream analysis by methods such as PCR- or qPCR-based assays, or massive parallel DNA sequencing.

ChIP
Chromatin immunoprecipitation (ChIP) is a powerful technique for studying the association of certain proteins with specific regions of the genome. These sequence-specific DNA-binding proteins are believed to play a role in such cellular processes as DNA replication, recombination, repair, and segregation; chromosomal stability; cell-cycle progression; and epigenetic silencing. In a standard ChIP assay, a cell is fixed via formaldehyde treatment and the chromatin is sheared and immunoprecipitated via a highly specific antibody. The researcher then analyzes the DNA to identify the genomic regions where the chromatin-associated proteins bind to the chromatin in vivo. This kit enables researchers to start with lower sample amounts than traditional ChIP workflows, thereby preserving precious samples, and the protocol can be completed in a single day, compared with 2–3 days for a traditional ChIP assay. The kit can be used with our suite of ChIP-validated antibodies, and is also complementary with MethylCode™ and NCode™ products for downstream epigenetics research.

Using the MAGnify™ system
When using the MAGnify™ system, you treat cells or tissue with formaldehyde to generate protein-protein and protein-DNA crosslinks between molecules in close proximity within the chromatin complex. The cells are then lysed, and the chromatin is released from the nuclei and sheared by sonication to reduce the average DNA fragment size to 200–500 bp for analysis by quantitative real-time PCR (qPCR) or 100–300 bp for analysis by massive parallel DNA sequencing. You then immunoprecipitate and isolate the crosslinked protein of interest using a specific ChIP-qualified antibody conjugated to Dynabeads® Protein A/G. The formaldehyde crosslinking is reversed by heat treatment, and the DNA associated with that protein is purified. The DNA is now ready for downstream analyses such as end-point PCR or quantitative PCR (qPCR), genome-wide analyses using promoter-tiling arrays, or next-generation sequencing. In PCR/qPCR analysis, primers are designed to span the desired DNA sequence of interest, and the data demonstrates whether the specific protein of interest is associated in vivo with that DNA region.
Results per page
    spinner