Shop All DNA⁄RNA Detection & Analysis Kits

EpiJET Bisulfite Conversion Kit (Thermo Scientific™)

Thermo Scientific EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged. Bisulfite converted unmethylated cytosines are detected as thymines in the following PCR.

The EpiJET Bisulfite Conversion Kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step. This step can be performed following long or short protocol, depending on the downstream application of modified DNA. The converted DNA is when bound to a membrane of a micro column for on-column desulfonation and subsequent DNA purification steps. The converted DNA is eluted in a small volume of Elution Buffer and is suitable for a number of techniques used for the methylation status analysis, including PCR, qPCR, COBRA, sequencing.

Highlights

• High conversion efficiency and specificity (≥99%)
• Flexibility, provided by short DNA conversion protocol
• Pure DNA, suitable for downstream applications and long-term storage

Applications

Converted DNA can be used in:

• PCR
• qPCR
• Combined Bisulfite Restriction Analysis (COBRA)
• Sequencing

Includes

• Modification Reagent
• Modification Solution I
• Modification Solution II
• Binding Buffer for Bisulfite Conversion Kit
• Wash buffer (conc.) for Bisulfite Conversion Kit
• Desulfonation Buffer (conc.)
• Elution Buffer
• DNA Purification Micro Columns & Collection Tubes

EpiJET DNA Methylation Analysis Kit (MspI/HpaII) (Thermo Scientific™)

5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.
The Thermo Scientific EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5'-CCGG-3' tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.

The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

Highlights

Fast—complete digestion of genomic DNA in 1 hour at 37°C
Efficient—specially formulated enzymes for epigenetic studies of DNA methylation status

Applications

• CpG methylation analysis at 5'-CCGG-3' loci

Includes

• Epi Mspl: 200 µL
• Epi HpaII: 200 µL
• 10X EpiBuffer: 1 mL
• Control pUC19/SmaI DNA Unmethylated (0.5 µg/ µL): 20 µL
• Control pUC19/SmaI DNA CpG Methylated (0.5 µg/ µL): 20 µL

Note

DNA sample quality is important for efficient digestion by restriction endonucleases or downstream applications. We recommend use of spin column based kits, such as the Thermo Scientific GeneJET Genomic DNA Purification Kit (#K0721, #K0722).

Precision gRNA Synthesis Kit (Invitrogen™)

The Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with TrueCut™ Cas9 Protein v2 for transfection-ready Cas9 protein/gRNA ribonucleoprotein (Cas9 RNP). This Cas9 RNP format, with our TrueCut Cas9 Protein v2, has been tested in a variety of suspension and adherent cell lines with >70% cleavage efficiencies and no indications of toxicity. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short ‘one-pot’ PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect Invitrogen™ CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.

Features of the Precision gRNA Synthesis Kit include:
• Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
• High yield (>10 ug) and concentration (>200 ng/uL) of gRNA

How to obtain a gRNA sequence
Genome editing with CRISPR technology requires a noncoding guide RNA (gRNA) in order to cleave genomic DNA at a target sequence of interest. The gRNA has two molecular components: a target-specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA) that have been combined into one transcript. The target sequence (20 bases) must be immediately upstream of a PAM motif (NGG) which allows the Cas9 to initiate binding. The PAM is only on the target DNA and not part of the target specific CRISPR sequence. The gRNA and the PAM motif guide the Cas9 nuclease to the target genomic sequence to form a complex and create a double-stranded blunt DNA break (DSB) three nucleotides upstream from the PAM site.

Use our CRISPR Search and Design Tool to search our database of >600,000 gRNA sequences specific to every gene in the human and mouse genomes. Invitrogen predesigned gRNAs are optimized for gene knockout and typically target the first three transcribed exons per gene. Search results include recommendations based on minimizing potential off-target effects, potential binding sites, and exon maps with gRNA locations. This tool can also be used to analyze any sequence of interest to design unique CRISPR sequences.

How to make gRNA
Once gRNA sequences have been selected, choose from three options for making gRNA:
1. TrueGuide synthetic guide RNA—choose from our catalog of predesigned gRNAs or upload your sequence to our TrueGuide gRNA Ordering Tool
2. Precision gRNA Synthesis Kit (this page)—for transfection-ready gRNA in as little as four hours including template assembly
3. Genome Engineering Services—save time and effort and have our custom services team design, synthesize, and purify in vitro transcribed (IVT) gRNA sequences for you. To obtain a services quotation, or to order, please contact our Custom Services department at 1-800-955-6288 x45682 or gemservices@thermofisher.com.

ViewRNA™ Tissue Assay Blue Module (Invitrogen™)

This VewRNA Tissue Assay Blue Module is designed for use with the ViewRNA Tissue Assay Core Kit. The Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).

Learn more about and order ViewRNA Tissue probe sets ›

ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.

With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets

The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue or OCT (optimal cutting temperature) embedded frozen tissue sections. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.

ViewRNA™ Tissue Assay Core Kit (Invitrogen™)

The ViewRNA Tissue Assay Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).

Learn more about and order ViewRNA Tissue probe sets ›

ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.

With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets

The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue or OCT (optimal cutting temperature) embedded frozen tissue sections. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.

TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific™)

Thermo Scientific TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 µL. Each reaction yields up to 200 µg RNA from 1 µg of template in 2 hours. The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA. All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.

Highlights

Exceptionally high yields—up to 200 µg in 2 hours
Versatile—suitable for both short and long RNA transcripts
Milligram amounts of RNA in a single, scaled-up reaction
Flexible—generates unlabeled, labeled or capped RNA
RiboRuler RNA Ladder supplied with kit for sizing and quantification

Applications

In vitro transcription
In vitro translation
• Generation of hybridization probes for:
• microarrays
in situ hybridization
• blotting

• RNase protection assays
• RNA binding protein assays
• Antisense RNA and RNAi
• RNA amplification
• Microinjection studies

Includes

• TranscriptAid Enzyme Mix
• 5X TranscriptAid Reaction Buffer
• DNase I, RNase-free
ATP Solution
CTP Solution
GTP Solution
UTP Solution
• Control template
• 3 M Sodium Acetate Solution, pH 5.2
• DEPC-treated Water
• 2X RNA Loading Dye
RiboRuler High Range RNA Ladder, ready-to-use
• 0.5 M EDTA, pH 8.0
• Detailed Protocol

* The improved version of TranscriptAid T7 High Yield Transcription Kit contains Tris-buffered NTPs instead of previously used NTPs titrated with KOH. Usage of Tris-buffered NTPs leads to higher yields of RNA transcripts.

TaqMan™ GMO Soy 35S Detection Kit, with protocol (Applied Biosystems™)

This kit contains reagents needed to reliably detect the presence of genetically modified organism (GMO)-specific DNA sequences in seed, grain, processed foods and their ingredients
• Universal assay detects and quantifies all approved genetically modified organisms (GMOs) in Europe and the vast majority of GMOs approved in other countries.
• Fluorogenic 5' nuclease assay using TaqMan® probes provides accurate and reproducible quantitative results.
• Label license conveys the PCR service rights for GMO testing when used in conjunction with an Authorized Thermal Cycler, so no additional royalties are payable.
• Comprehensive controls provide high confidence in results.
• Innovative GMO quantitation software allows users to quickly and easily determine the percentage of GMO content.
• Offers the complete solution including sample preparation, instruments, reagents, analysis software, technical support, instrument service, and GMO expertise.

The Complete Solution
The TaqMan® GMO Detection and Quantitation Kit is the first of a full product line of GMO assays in development that can detect the presence of GMO-specific DNA sequences in seed, grain, and processed foods and their ingredients. The TaqMan® GMO kit is part of a highly sophisticated but user-friendly system that includes DNA sample preparation, automated PCR amplification and signal detection, data analysis, and quantitation software, thus eliminating the need for personnel trained in molecular techniques.

Reliable TaqMan® Chemistry
The system uses the patented fluorogenic 5' nuclease method for multiplex detection and quantitation, the latest innovation in real-time PCR technology. It targets the Cauliflower Mosaic Virus 35S promoter, a GMO-specific sequence present in all GMO soy and maize events approved for use in food by the European Union, and in the vast majority of GMO events approved by other countries. Both the European Union and Japan view this method as the premier technology for quantitating GMO content in food. Other TaqMan® kits are available for the detection and quantitation of food-borne pathogens, including Salmonella and E. coli 0157:H7.

For Research Use Only. Not for use in diagnostics procedures.

mirVana™ miRNA Detection Kit (Invitrogen™)

The Ambion® mirVana™ miRNA Detection Kit provides a fast and sensitive method for detecting small RNAs. The assay is 100–500 times more sensitive than Northern analysis, it is able to detect as little as 10 attomoles (10-17 mol) of target RNA. Each kit includes sufficient reagents for 100 reactions.

• Sensitive—detect miRNA or siRNA in as little as 50 ng total RNA
• Specific—extremely low backgrounds
• Simple and fast—single-tube procedure eliminates the need to transfer to membrane for hybridization
• Multiple target detection—detect multiple small RNAs and mRNAs in the same sample

Rapid Procedure Based on Solution Hybridization
The mirVana™ miRNA Detection Kit is based on a simple solution hybridization principle where the RNA sample is simply mixed with radiolabeled RNA probe(s) and hybridized. Unhybridized RNA and excess probe are removed by a digestion step and the hybridized, protected RNA fragments are then recovered using a patented single-step technology for simultaneous ribonuclease inactivation and nucleic acid precipitation. RNA samples are then resuspended and analyzed on a denaturing polyacrylamide gel.

Simultaneous Detection of siRNA Expression and Target Gene Knockdown
Because multiple RNA species of the same or differing size can be detected in the same sample, the mirVana™ miRNA Detection Kit is ideal for correlating siRNA expression levels with target mRNA knockdown. An example of this application is shown in the accompanying data, which demonstrates that GAPDH mRNA levels were reduced in cells expressing a GAPDH siRNA, but not in cells expressing a control siRNA.

Accessory Products:
The mirVana™ miRNA Probe Construction Kit (SKU#AM1550) is available for the preparation and labeling of probes by in vitro transcription and the mirVana™ Probe & Marker Kit is available for 5' end labeling of oligonucleotide probes and the included Decade Markers (SKU#AM1554).

ViewRNA™ ISH Cell Accessory Kit (Invitrogen™)

The ViewRNA™ ISH Cell Accessory Kit is intended to provide many of the required components, not supplied in reagent kits, in order to perform the ViewRNA™ ISH Cell Assay.

Reported Applications:
Microscopy

MEGAshortscript™ T7 Transcription Kit (Invitrogen™)

The MEGAshortscript™ T7 Kit is designed for high yields of in vitro- transcribed RNA products, in the 20–500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.

Advantages of the MEGAshortscript™ Kit:

• Efficiently transcribes small templates (<300 bases) with consistently high yields
• Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates

Note:The MEGAshortscript™ T7 Kit is optimized for use with the T7 polymerase included in the kit. Using a different polymerase may result in low yields.

NorthernMax™-Gly Gel Prep/Running Buffer (10X) (Invitrogen™)

For the preparation and running of agarose gels. For use with RNA denatured by glyoxal treatment; one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.

TaqMan™ GMO Maize 35S Detection Kit, with protocol (Applied Biosystems™)

This kit contains reagents needed to reliably detect the presence of genetically modified organism (GMO)-specific DNA sequences in seed, grain, processed foods and their ingredients.

• Universal assay detects and quantifies all approved genetically modified organisms (GMOs) in Europe and the vast majority of GMOs approved in other countries
• Fluorogenic 5' nuclease assay using TaqMan® probes provides accurate and reproducible quantitative results
• Label license conveys the PCR service rights for GMO testing when used in conjunction with an Authorized Thermal Cycler, so no additional royalties are payable
• Comprehensive controls provide high confidence in results
• Innovative GMO quantitation software allows users to quickly and easily determine the percentage of GMO content
• Offers the complete solution including sample preparation, instruments, reagents, analysis software, technical support, instrument service, and GMO expertise

The Complete Solution
The TaqMan® GMO Detection and Quantitation Kit is the first of a full product line of GMO assays in development that can detect the presence of GMO-specific DNA sequences in seed, grain, and processed foods and their ingredients. The TaqMan® GMO kit is part of a highly sophisticated but user-friendly system that includes DNA sample preparation, automated PCR amplification and signal detection, data analysis, and quantitation software, thus eliminating the need for personnel trained in molecular techniques.

Reliable TaqMan® Chemistry
The system uses the patented fluorogenic 5' nuclease method for multiplex detection and quantitation, the latest innovation in real-time PCR technology. It targets the Cauliflower Mosaic Virus 35S promoter, a GMO-specific sequence present in all GMO soy and maize events approved for use in food by the European Union, and in the vast majority of GMO events approved by other countries. Both the European Union and Japan view this method as the premier technology for quantitating GMO content in food. Other TaqMan® kits are available for the detection and quantitation of food-borne pathogens, including Salmonella and E. coli 0157:H7.

For Research Use Only. Not for use in diagnostics procedures.