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FCGR3A (CD16a) Recombinant Human Protein, 176 Val, His Tag

Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human FCGR3A (P08637-1), containing the mutation 176 Phe/Val, precursor fused to a polyhistidine tag at the C-terminus.

Activity: Measured using the Octet RED System, the affinity constant (Kd) of FCGR3A bound to human IgG1 was 180nM.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

Human CD16a/FCGR3a Recombinant Protein, 176 Phe, His & Avi tag

Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1- Gln 208) of human FCGR3A (P08637-1) fused to a polyhistidine tag at the C-terminus.

Activity: Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 180nM.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

RARG Recombinant Human Protein, Ligand Binding Domain, GST-Tagged

Retinoic acid receptor gamma, ligand binding domain (RARG-LBD) is a 58.85 kDa recombinant human protein (amino acids 178-454) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells. RARG-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator recruitment studies.

CEACAM-5 Recombinant Human Protein, His Tag

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 Protein (CEACAM5), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human CEACAM5 (NP_004354.2) (Met 1-Ala 685) fused to a polyhistidine tag at the C-terminus.

Activity: The activity of this protein has not been tested.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

PPARG Recombinant Human Protein, Ligand Binding Domain, GST-Tagged

PPARG (peroxisome proliferator activated receptor gamma) ligand binding domain a 59.00 kDa recombinant human protein (amino acids 201-475) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells. PPAR γ-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

Glucocorticoid Receptor Recombinant Human Protein

Glucocorticoid receptor recombinant protein is an insect cell-expressed, full-length, untagged, human nuclear hormone receptor that is partially purified in order to maintain high ligand binding activity. Further purification results in decreased activity, presumably due to loss of endogenous accessory proteins. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity.

GR human recombinant protein is:

• Flexible—use in your in-house assay or as part of our PolarScreen™ Glucocorticoid Receptor Competitor assays for a complete assay solution
• Ready to use—active and native (untagged)
• Convenient—available in bulk sizes

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Used in:
PolarScreen™ Glucocorticoid Receptor Competitor Assay, Red
PolarScreen™ Glucocorticoid Receptor Competitor Assay, Green

RARA Recombinant Human Protein, Ligand Binding Domain, GST-Tagged

Retinoic acid receptor alpha, ligand binding domain (RARA-LBD) is a 59.53 kDa recombinant human protein (amino acids 176-462) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells. RARA-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator recruitment studies.

Progesterone Receptor Recombinant Human Protein, Ligand Binding Domain

Progesterone receptor ligand binding domain (PR-LBD) is a 59.83 kDa recombinant human protein (amino acids 675-933) expressed as a glutathione-S-transferase (GST) fusion protein in baculovirus-infected insect cells. PR is expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

PR-LBD human recombinant protein is:

• Flexible—use in your in-house assay or as part of our PolarScreen™ Competitor assays for a complete assay solution
• Ready to use—active and tagged (with GST)
• Convenient—available in bulk sizes

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Used in:
PolarScreen™ Progesterone Receptor Competitor Assay, Red
PolarScreen™ Progesterone Receptor Competitor Assay, Green

MET (c-MET) Recombinant Human Protein (without Catalytic Activity Domain), His Tag

Met proto-oncogene/hepatocyte growth factor receptor (MET/c-MET), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies..

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human MET (NP_000236) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 10 µg/ml (100 µl/well) can bind biotinylated MET. The ED50 of biotinylated Met is 5.28 µg/ml.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

ER Beta Recombinant Protein, full length

Estrogen receptor (ER) beta is an insect cell-expressed, full-length, untagged, human nuclear hormone receptor that binds estrogens with an affinity similar to that of ER alpha.

ER beta full-length recombinant protein is:

• Flexible—use in your in-house assay or as part of our PolarScreen™ ER Beta Competitive Binding Assay for a complete assay solution
• Ready to use—active, purified, and native (untagged)
• Convenient—available in bulk sizes

ER beta recombinant protein is manufactured under the strictest quality parameters. This product is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

ESRRG Recombinant Human Protein, Ligand Binding Domain, GST-Tagged

ESRRG (estrogen-related receptor gamma) ligand binding domain is a 54.05 kDa recombinant human protein (amino acids 229-458) expressed as a GST-tagged fusion protein in baculovirus-infected insect cells. ERR γ-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

ER Alpha Recombinant Protein, full length

ER (Estrogen Receptor) Alpha Recombinant Protein is an insect cell-expressed, full-length, untagged, human nuclear hormone receptor that binds [3H]-estradiol with sub-nM affinity in hydroxylapatite assays and binds specifically to estrogen response element (ERE) sequences.

ER alpha full-length recombinant protein is:

• Flexible—use in your in-house assay or as part of our PolarScreen™ ER Alpha Competitor assays for a complete assay solution
• Ready to use—active, purified, and native (untagged)
• Convenient—available in bulk sizes

ER Alpha Recombinant Protein is manufactured under the strictest quality parameters. This product is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Used in:
PolarScreen™ ER Alpha Competitor Assay, Red
PolarScreen™ ER Alpha Competitor Assay, Green

ER Beta Recombinant Protein, ligand binding domain, GST tag

This Estrogen Receptor (ER) Beta Ligand Binding Domain (LBD) is a 59.50 kDa recombinant human protein (amino acids 244-530) expressed as a GST fusion protein in baculovirus-infected insect cells. It was expressed and purified in the absence of exogenous ligands.

ER Beta-LBD recombinant protein is:
• Flexible—ideal for ligand binding and coregulator displacement assays developed in-house or partnered with our LanthaScreen® ER Beta Coacvitator Assay Kit
• Ready to use—active, purified, and tagged with GST
• Convenient—available in bulk sizes

ER Beta-LBD recombinant protein is manufactured under the strictest quality parameters. This product is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Used in:
LanthaScreen® TR-FRET ER Beta Coactivator Assay Kit

For bulk reagents, email: !Mad-DiscoveryServices@lifetech.com

CD64 Recombinant Human Protein, His Tag

CD64 (FCGR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human FCGR1A (NP_000557.1) (Met1-Pro288) was expressed with a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD64 Protein (Cat:10256-H08S) at 10 µg/mL can bind human IgG1, The EC50 of human IgG1 is 6-14 ng/mL.

Formulation: Lyophilized from sterile PBS (pH7.4), 5%-8% Mannitol, 5%-8% Trehalose, and 0.01% Tween-80 added prior to lyophilization.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

Androgen Receptor Recombinant Rat Protein, Ligand Binding Domain

Androgen receptor ligand binding domain (AR-LBD) is a 64.42 kDa recombinant rat protein, expressed as a GST- and His-tagged protein in insect cells. The protein contains the hinge region and an LBD amino acid sequence identical to that of the human LBD. Differences in the rat and human hinge region are present at human amino acids 646, 649, 650, 655, 656, 657, and 660. AR-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand-binding and coregulator recruitment studies.

AR-LBD rat recombinant protein is:

• Flexible—use in your in-house assay or as part of our PolarScreen™ Competitor assays for a complete assay solution
• Ready to use—active and tagged (with GST/His)
• Convenient—available in bulk sizes

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
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