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AMPK (A1/B2/G3) Recombinant Human Protein (Invitrogen™)

This AMPK (A1/B2/G3) recombinant human protein (full length) was expressed in insect cells. AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha1, beta2 and gamma3 subunits. While the purified AMPK (A1/B2/G3) complex can be used in a number of different applications, it has been specifically validated for use with our Z’-LYTE™ assay technology to identify novel kinase inhibitors. AMPK (A1/B2/G3) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

AMPK (A2/B1/G2) Recombinant Human Protein (Invitrogen™)

This AMPK (A2/B1/G2) recombinant human protein (full length) was expressed in insect cells. AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha2, beta1 and gamma2 subunits. While the purified AMPK (A2/B1/G2) complex can be used in a number of different applications, it has been specifically validated for use with our Z’-LYTE™ assay technology to identify novel kinase inhibitors. AMPK (A2/B1/G2) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

AMPK (A2/B1/G3) Recombinant Human Protein (Invitrogen™)

This AMPK (A2/B1/G3) recombinant human protein (full length) was expressed in insect cells. AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha2, beta1 and gamma3 subunits. While the purified AMPK (A2/B1/G3) complex can be used in a number of different applications, it has been specifically validated for use with our Z’-LYTE™ assay technology to identify novel kinase inhibitors. AMPK (A2/B1/G3) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

RET S891A Recombinant Human Protein (Invitrogen™)

This RET S891A recombinant human protein (658–end) was expressed in insect cells. RET S891A receptor tyrosine kinase or ret proto-oncogene is a member of the cadherin superfamily which are cell-surface molecules that transduce signals for cell growth and differentiation. RET can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in the RET gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. RET signaling pathway, by regulating the development of both the nervous and lymphoid system in the gut, plays a key role in the molecular mechanisms that orchestrate intestine organogenesis. RET S891A recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

AMPK (A2/B2/G3) Recombinant Human Protein (Invitrogen™)

This AMPK (A2/B2/G3) recombinant human protein (full length) was expressed in insect cells. AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha2, beta2 and gamma3 subunits. While the purified AMPK (A2/B2/G3) complex can be used in a number of different applications, it has been specifically validated for use with our Z’-LYTE™ assay technology to identify novel kinase inhibitors. AMPK (A2/B2/G3) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

SBK1 Recombinant Human Protein (Invitrogen™)

This SBK1 recombinant human protein (full length) was expressed in insect cells. SBK1 novel serine/threonine kinase or SH3 domain binding kinase 1 is structurally related to a Xenopus gastrula-specific protein kinase, Pk9.7 which possessing a consensus sequence for an SH3-binding domain from developing rat brain. SBK1 plays an important role in signal-transduction pathways related to the control of brain development. SBK1 may predict a broad cellular function, and its dysregulated in certain cancers suggests an involvement of the protein in the pathogenesis of human cancers. SBK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

CAMK1G (CaMKI gamma) Recombinant Human Protein (Invitrogen™)

This CAMK1G (CaMKI gamma) recombinant human protein (full length) was expressed in insect cells. CAMK1G serine/threonine kinase is a member of CAMK family and is a novel membrane-anchored neuronal Ca2+ calmodulin-dependent protein kinase. Full activation of CAMK1G requires both Ca2+ and calmodulin and phosphorylation by CAMKK. CAMK1G transcripts are most abundant in neurons, with the highest levels in limited nuclei such as the central nucleus of the amygdala (CeA) and the ventromedial hypothalamus. CAMK1G (CaMKI gamma) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

TEK (TIE2) Y897S Recombinant Human Protein (Invitrogen™)

This TEK (TIE2) Y897S recombinant human protein (771–end) was expressed in insect cells. TEK (TIE2) Y897S receptor tyrosine kinase is expressed principally on vascular endothelium. Disrupting TEK Y897S function in mice results in embryonic lethality with defects in embryonic vasculature, suggesting a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TEK receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TEK kinase domain and are part of the cellular responses that mediate TEK function. TEK expression is upregulated in the endothelium of vascular "hot spots" in human breast cancer specimens. However, TEK is also overexpressed in areas of active angiogenesis in normal tissues. TEK (TIE2) Y897S recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

CDC42 BPG (MRCKG) Recombinant Human Protein (Invitrogen™)

This CDC42 BPG (MRCKG) recombinant human protein (1–475) was expressed in insect cells. CDC42 BPG serine/threonine kinase is encoded by the CDC42BPG gene. It acts as a downstream effector of CDC42 in cytoskeletal reorganization, and contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and MLC2 phosphorylation. It belongs to the AGC protein kinase family, DMPK subfamily. It contains an N-terminal kinase domain, followed by a coiled-coil domain, a second coiled-coil domain containing a kinase inhibitory motif (KIM), a variable region, a cysteine-rich domain, a pleckstrin (PLEK; 173570) homology (PH) domain, a citron (605629) homology (CNH) domain, and a C-terminal CDC42 (116952)/RAC (602048) interactive binding (CRIB) domain. CDC42 BPG (MRCKG) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

TNK1 Recombinant Human Protein (Invitrogen™)

This TNK1 recombinant human protein (1–510) was expressed in insect cells. TNK1 tyrosine kinase, non-receptor 1 belongs to the tyrosine protein kinase family and as such is an important regulator of intracellular signal transduction pathways mediating cellular proliferation, survival, and development. TNK1 is highly expressed in fetal tissues and at lower levels in a few adult tissues. TNK1 may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. TNK1 plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. TNK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

HCK Human Protein

This recombinant fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
Expressed in insect cells
GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format, with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer’ to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

TGFBR2 for the LanthaScreen Kinase Binding Assay
Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).

This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
 Tracer 236 (Cat No. PV5592)
•  Eu-labeled GST Antibody (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.

CDK17/cyclin Y Recombinant Human Protein (Invitrogen™)

This CDK17/cyclin Y recombinant human protein (CDK17: full length, cyclin Y: 2–end) was expressed in insect cells. CDK17/cyclin Y serine/threonine kinase or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of kinases. CDK17/cyclin Y has similarity to a rat protein that is thought to play a role in terminally differentiated neurons. The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins. CDK17/cyclin Y recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

PKMYT1 Recombinant Human Protein (Invitrogen™)

This PKMYT1 recombinant human protein (full length) was expressed in insect cells. PKMYT1 serine/threonine kinase is a member of a family of neural specific, zinc finger-containing DNA-binding proteins which binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system. MYT1 of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. The recombinant PKMYT1 fragments containing either the upstream 2 zinc fingers or the downstream 4 zinc fingers bound the same cis regulatory element in the PLP1 promoter in vitro and DNA binding required Zn2+, but not other divalent cations. PKMYT1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen™ Eu Kinase Binding assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

MAP4K2 (GCK) Recombinant Human Protein

MAP4K2 (GCK) is a serine⁄ threonine protein kinase that activates the JNK pathway. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of the germinal center, where it may participate in B-cell differentiation. The kinase may also play a role in the regulation of vesicle targeting or fusion.

CSNK2A2 (CK2 α 2) Recombinant Human Protein

Casein kinase II is a protein serine⁄threonine kinase that is thought to be present in all eukarotic cells, implying that it has fundamental cellular functions. The holoenzyme is a tetramer containing 2 alpha or alpha-prime subunits (or one of each) and 2 beta subunits. The function of beta subunits is unknown and the alpha subunit is the catalytic unit.