Shop All Kinases

STK32B (YANK2) Recombinant Human Protein

STK32B or YANK2 is a serine/threonine protein kinase that contains motif for binding metal ions and nucleotides and the gene for STK32B is highly conserved among various species. STK32B has been associated with isolated cleft lip with or without cleft palate and cleft palate which are among the most common human birth defects of oral cleft cases. Long interspersed nuclear element-1 (LINE-1 or L1) mediated deletion of STK32B gene is observed in patients with Ellis-van Creveld syndrome with borderline intelligence. Full-length recombinant human STK32B (YANK2) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

STK32C (YANK3) Recombinant Human Protein

STK32C or YANK3 is a member of the YANK family and is a serine/threonine protein kinase of approximately 486 aa that requires magnesium for catalytic activity. There are two isoforms of the protein generated by alternative splicing and the protein undergoes serine phosphorylation by upstream protein kinases. The STK32C gene product is conserved in many species including chimpanzee, dog, cow, mouse, rat, and chicken. Recombinant human STK32C (YANK3) (66-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

TNIK Recombinant Human Protein

TNIK or TRAF2 and NCK interacting kinase is characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function. TNIK is mainly expression in brain, heart, and spleen and it is a specific effector of RAP2 which regulate actin cytoskeleton. TNIK is autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain and plays a main role in cytoskeleton regulation. Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

STK39 (STLK3) Recombinant Human Protein

STK39 (STLK3) is involved in the cellular stress response pathway. STK39 is activated in response to hypotonic stress leading to phosphorylation of several cation-chloride-coupled co-transporters. STK39 activates the p38 MAP kinase pathway and its interaction with p38 decreases during cellular stress. STK39 acts as an intermediate in the response to cellular stress. STK39 is also an independent risk factor for hypertension in men and its intragenic SNPs can interact and function in the control of blood pressure. Recombinant full-length human STK39 (STLK3) was expressed using baculovirus in Sf9 insect cells using an N-terminal His tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

IRAK1 Recombinant Human Protein

IL-1 receptor associated kinases (IRAKs) are serine⁄threonine kinases involved in signaling via Toll⁄IL-1 pathways.

LRRK2 Recombinant Human Protein

LRRK2 is a multidomain protein that includes a kinase domain, a GTPase domain, and a leucine-rich repeat (LRR) domain. Mutations in this gene have been associated with Parkinson's disease.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay

AMPK (A2/B1/G2) Recombinant Human Protein (Invitrogen™)

This AMPK (A2/B1/G2) recombinant human protein (full length) was expressed in insect cells. AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha2, beta1 and gamma2 subunits. While the purified AMPK (A2/B1/G2) complex can be used in a number of different applications, it has been specifically validated for use with our Z’-LYTE™ assay technology to identify novel kinase inhibitors. AMPK (A2/B1/G2) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

PIK3CD/PIK3R1 (p110 delta/p85 alpha) Recombinant Human Protein, His tag

Phosphatidylinositol 3-kinase (PI3K) is composed of a catalytic subunit (p110δ) and a regulatory subunit (p85α in this case), which are encoded by the PIK3CD and PIK3R1 genes. PI3K phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It plays a role in oncogenic transformation and is consistently expressed at a high level in AML blast cells. PI3K can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that leads to neutrophil polarization, and regulates directional migration, but not random locomotion. The PI3K signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.

PIK3CD/PIK3R1 (p110δ/p85α) recombinant human protein is:
• Available in 3 convenient sizes
• Validated in our Adapta™ and Kinase Binding assays
• A full length protein that can help advance your research when used with validated assays

Related assay platforms:
LanthaScreen® Kinase Binding Assay
Adapta® Kinase Activity Assay

ULK1 Recombinant Human Protein

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

MAP2K1 (MEK1) [S218D] [S222D] Recombinant Human Protein

MAP2K1 (MEK1) kinse is a dual-specific protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. This mutant has been activated by subsituting serines 218 and 222 with aspartic acid to mimic the phosphorylated state.

PAK7 (KIAA1264) Recombinant Human Protein

PAK7 (KIAA1264) is implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling.

STK17A (DRAK1) Recombinant Human Protein

STK17A (DRAK1) is a serine⁄threonine kinase localized in the nucleus and involved in apoptosis.

MAP3K3 (MEKK3) Recombinant Human Protein

MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks.

AMPK (A2/B1/G3) Recombinant Human Protein (Invitrogen™)

This AMPK (A2/B1/G3) recombinant human protein (full length) was expressed in insect cells. AMPK (AMP-activated protein kinase) serine/threonine kinase is a heterotrimeric complex, which acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulation of metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. This AMPK consists of the alpha2, beta1 and gamma3 subunits. While the purified AMPK (A2/B1/G3) complex can be used in a number of different applications, it has been specifically validated for use with our Z’-LYTE™ assay technology to identify novel kinase inhibitors. AMPK (A2/B1/G3) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

PIK3CB/PIK3R1 (p110 beta/p85 alpha) Recombinant Human Protein

PIK3CB/PIK3R1 (p110β/p85α) is a phosphoinositide-3-kinase (PI3K) comprised of a catalytic subunit, p110β, and a regulatory subunit, p85α, encoded by the PIK3CB and PIK3R1 genes respectively. As with other members of the PI3K family, p110β has important regulatory roles in cellular processes including cell survival, proliferation, and differentiation. It affects growth factor signaling and survival by phosphorylating inositol lipids. PTEN-deficient cancer cells have been observed to depend on p110β signaling.

PIK3CB/PIK3R1 (p110β/p85α) Recombinant Human Protein is:
• Available in 3 convenient sizes
• Validated in our Adapta® and Kinase Binding assays
• A full-length protein that can help advance your research when used in validated assays

Related assay platforms:
LanthaScreen® Kinase Binding Assay
Adapta® Kinase Activity Assay