Shop All Kinases

TLK2 Recombinant Human Protein

TLK2 or Tousled-like kinase 2, is a nuclear serine/threonine kinases that is thought to be involved in the regulation of chromatin assembly. In addition, TLK2 is also involved in other cellular processes such as intracellular signaling cascade, protein amino acid phosphorylation, cell cycle, chromatin modification and in response to DNA damage stimulus. TLK2 displays maximal activity during S phase of the cell cycle where it appears to be regulated by cell-cycle-dependent phosphorylation. Inhibition of DNA replication causes a rapid loss of TLK2 activity, indicating that TLK2 function is tightly linked to ongoing DNA replication. Recombinant human TLK2 (388-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

MAP3K7/MAP3K7IP1 (TAK1-TAB1) Recombinant Human Protein

MAP3K7 (TAK1) controls transcription regulation and apoptosis, and is implicated in inflammatory disease and rheumatoid arthritis. Fusion of MAP3K7 to MAP3K7IP1 (TAB1) activates MAP3K7.

ANKK1 Recombinant Human Protein (Invitrogen™)

This ANKK1 recombinant human protein (full length) was expressed in insect cells. ANKK1 serine/threonine kinase is a member of the RIPK family and is closely linked to the DRD2 gene. The recombinant full length ANKK1 can be used in a number of different applications, but has been specifically validated for use with our LanthaScreen™ Kinase binding assay technology to identify novel kinase inhibitors. ANKK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen™ Eu Kinase Binding assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

CSNK1E (CK1 epsilon) R178C Recombinant Human Protein (Invitrogen™)

This CSNK1E (CK1 epsilon) R178C recombinant human protein (full length) was expressed in insect cells. CSNK1E serine/threonine kinase is a member of the CK1 family which plays an important role in diverse cell processes, including DNA replication and repair. CSNK1E is a regulator of Yes-associated protein (YAP) transcription coactivator, which is a key regulator of organ size and a candidate human oncogene. CSNK1E is activated by CCK2R and then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. CSNK1E can phosphorylate topoisomerase (topo) II alpha at serine-1106 and this regulates the enzyme activity and sensitivity to topo II-targeted drugs. CSNK1E (CK1 epsilon) R178C recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

NEK2 Recombinant Human Protein

NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation.

DCAMKL2 (DCK2) Recombinant Human Protein

DCAMKL2 is a serine⁄threonine kinase involved in signal transduction and cellular communication, and is the human homolog of a rat protein kinase expressed in cerebrocortical lamination that binds microtubules.

EIF2AK1 (HRI) Human Protein

EIF2AK1 (HRI) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK1, signaling is initiated by low levels of heme.

EPHA4 Recombinant Human Protein

EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system.

DAPK3 (ZIPK) Recombinant Human Protein

DAPK3 is a serine⁄threonine kinase which is functionally linked to cell motility, smooth muscle contractility and cell death processes.

GRK6 Recombinant Human Protein

GRK6 is a member of the guanine nucleotide-binding protein coupled receptor kinase subfamily of the serine⁄threonine protein kinase family. GRK6 phosphorylates the activated forms of G protein-coupled receptors to initiate their deactivation.

MAPK12 (p38 γ) Recombinant Human Protein

MAPK12 (p38 gamma) is a serine⁄threonine kinase involved in many different cellular processes. It is activated during hypoxia and gamma-irradiation-induced G(2) arrest.

BMPR1B (ALK6) Recombinant Human Protein

BMPR1B (also known as ALK6) is a member of the transmembrane serine/threonine kinase that is the member of the bone morphogenetic protein (BMP) receptor, which is closely related to the activin receptors, ACVR1 and ACVR2. BMPR1B is mainly involved in the endochondral bone formation and embryogenesis. BMPR1B expressed in normal and cancerous prostate tissues and used in the endocrine therapy that given to the prostate cancer patients. BMPR1B receptor trafficking also play a significant role in FOP pathogenesis and used in human T-cell differentiation. Recombinant human BMPR1B (149-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Recombinant human BMPR1B (149-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

CLK4 Recombinant Human Protein

CLK4 belongs to the CDC2-like protein kinase (CLK) family. It can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus is thought to be involved in the regulation of alternative splicing.

TEC Recombinant Human Protein

TEC is a non-receptor protein-tyrosine kinase containing a pleckstrin homology domain and is highly expressed in many hematopoietic cell lines.

TGFBR2 Recombinant Human Protein

This recombinant fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
Expressed in insect cells
GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format, with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer’ to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

TGFBR2 for the LanthaScreen Kinase Binding Assay
Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).

This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
 Tracer 178 (Cat No. PV5593)
•  Eu-labeled GST Antibody (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.

TAOK3 (JIK) Recombinant Human Protein

TAOK3 (JIK) is a serine⁄threonine kinase that is a member of the GCK-like subfamily of the STE20 kinase family. JIK is ubiquitously expressed in human tissues, inhibits JNK and has been implicated in cancer.

PDGFRA [T674I] Recombinant Human Protein

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. PDGFRA T674I is a Gleevec resistant form of PDGFRA.

IRAK1 Recombinant Human Protein

IL-1 receptor associated kinases (IRAKs) are serine⁄threonine kinases involved in signaling via Toll⁄IL-1 pathways.

JAK1 Recombinant Human Protein

Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha⁄beta and -gamma signal transduction pathways.

AKT2 (PKB beta) Recombinant Human Protein

AKT2 is a phosphoinositide-dependent serine⁄threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone.

INSR Recombinant Human Protein

INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake.

MARK4 Recombinant Human Protein

MARK4 is a serine⁄threonine kinase that is likely to be directly involved in microtubule organization in neuronal cells.

CDK13/cyclin K Recombinant Human Protein (Invitrogen™)

This CDK13/cyclin K recombinant human protein (CDK13: 656–1050, cyclin K: 1–300) was expressed in insect cells. CDK13/cyclin K serine/threonine kinase is dimeric protein complex. CDK13 is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are well known for their essential roles as master switches in cell cycle control. The exact function of this protein has not yet been determined, but it may play a role in mRNA processing and may be involved in regulation of hematopoiesis. Cyclin K is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain of the large subunit of RNA polymerase II. CDK13/cyclin K recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen™ Eu Kinase Binding assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

MAP3K3 (MEKK3) Recombinant Human Protein

MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks.

CAMK1 (CaMK1) Recombinant Human Protein

CAMK1 (CaMK1) is a serine⁄threonine kinase that is a member of the calcium⁄calmodulin-dependent protein kinase family. It is involved in cell differentiation, nervous system development, and signal transduction.

TTK Recombinant Human Protein

TTK is a serine⁄threonine kinase closely related to SPK1, PIM 1 and CDC2 serine⁄threonine kinases. TTK is a dual-specificity kinase, which can also phosphorylate tyrosine.

PRKCQ (PKC theta) Recombinant Human Protein

The PKC family of serine⁄threonine kinases, including PRKCQ (PKC theta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.

CDC42 BPA (MRCKA) Recombinant Human Protein

CDC42BPA (MRCKA) is a serine⁄threonine kinase which functions in actin cytoskeleton assembly and organization.

MET [D1228H] Recombinant Human Protein

Human MET C-terminal fragment, amino acids K956-S1390 (as in GenBank entry NM_000245.2), D1228H mutant, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

PAK2 (PAK65) Recombinant Human Protein

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine⁄threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities.