Shop All Serine⁄Threonine Kinases

WNK1 Recombinant Human Protein Invitrogen™

This WNK1 recombinant human protein (171–529) was expressed in insect cells. WNK1 serine/threonine kinase is a member of the WNK subfamily. WNK1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen™ Eu Kinase Binding assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

STK4 (MST1) Recombinant Human Protein

STK4 (MST1) is a serine⁄threonine kinase which may be involved in apoptosis. It is possible that this protein induces the chromatin condensation observed in this process.

DAPK1 Recombinant Human Protein

DAPK1 is a large, calmodulin-dependent kinase involved in gamma-interferon induced programmed cell death and is a potential tumour suppressor.

RPS6KA1 (RSK1) Recombinant Human Protein

RPS6KA1 (RSK1) is a growth-factor regulated serine⁄threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors.

CDK4/cyclin D3 Recombinant Human Protein Invitrogen™

This CDK4/cyclin D3 recombinant human protein (CDK4: full length, cyclin D3: full length) was expressed in insect cells. CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein. CDK4/cyclin D3 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

PIM2 Recombinant Human Protein

PIM2 is a proto-oncogene which phosphorylates BAD, which partially inhibits its apopotopic function.

MAPK12 (p38 γ) Recombinant Human Protein

MAPK12 (p38 gamma) is a serine⁄threonine kinase involved in many different cellular processes. It is activated during hypoxia and gamma-irradiation-induced G(2) arrest.

ULK3 Recombinant Human Protein

ULK3 or unc-51-like kinase 3 is a member of the serine/threonine kinase family that is involved in the SHH signaling pathway as a positive regulator of GLI proteins. ULK3 enhances endogenous and over-expressed GLI transcriptional activity in cultured cells and alters its subcellular localization. Furthermore, ULK3 phosphorylates GLI proteins in vitro. ULK3 is widely expressed and its expression is higher in a number of tissues where SHH signaling is known to be active, again suggesting that ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins. Full-length recombinant human ULK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

LATS2 Recombinant Human Protein

LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. LATS2 interacts with a negative regulator of p53 and function in a positive feedback loop with p53 that responds to cytoskeleton damage and this interaction provokes centrosome/mitotic apparatus dysfunction. LATS2 plays an essential role in the maintenance of mitotic fidelity and genomic integrity. Recombinant human LATS2 (480-1088) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

CDK8/Cyclin C Recombinant Human Protein

CDK8 phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II. This protein is coexpressed and copurified with CyclinC.

AURKC (Aurora C) Recombinant Human Protein

AURKC (Aurora C) is a chromosomal passenger protein that forms complexes with AURKB (Aurora B) and inner centromere proteins, playing a role in organizing microtubules in relation to centrosome⁄spindle function during mitosis. It is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction.

RPS6KA2 (RSK3) Recombinant Human Protein Invitrogen™

RPS6KA2 (RSK3) serine/threonine kinase is a member of the ribosomal S6 kinase (RSK) family. It is believed that RPS6KA2 is a signal-transducing intermediate in the cellular response to several growth factors. It is found in many tissues, but in higher levels in lung and skeletal muscle.

The purified recombinant full-length protein can be used in a number of different applications, but it has been specifically validated for use with both the LanthaScreen™ & Z'-LYTE™ Assay technologies to identify novel kinase inhibitors. RPS6KA2 is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen & Z'-LYTE assay technology
• Available in multiple pack sizes for convenient use
• Also available in our SelectSceen Kinase Profiling Service

STK24 (MST3) Recombinant Human Protein

STK24 (MST3) is a serine⁄threonine kinase that is a member of the GCK subfamily of STE20-like proteins.

MAPK13 (p38 δ) Recombinant Human Protein

MAPK13 (p38 delta) is a serine⁄threonine kinase that transduces a variety of extracellular signals, including cytokine and environmental stress responses. MAPK13 also regulates the differentiation of keratinocytes.

SIK1 Recombinant Human Protein

SIK1 is a protein kinase that is involved in regulating AMPK-related kinases. This recombinant human SIK1 (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance

SIK1 may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells.

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Related Links:
LanthaScreen® Kinase Activity Assay
LanthaScreen® Eu Kinase Binding Assay
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