Shop All Cytoplasmic Tyrosine Kinases

ITK Recombinant Human Protein

ITK is a tyrosine kinase expressed in T-cells which contains both SH2 and SH3 domains. ITK is thought to play a role in T-cell proliferation and differentiation.

ABL1 F317L Recombinant Human Protein (Invitrogen™)

This ABL1 F317L recombinant human protein (27–end) was expressed in insect cells. ABL1 cytoplasmic tyrosine kinase protooncogene encodes a cytoplasmic and nuclear kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of ABL protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene. Translocation and head-to-tail fusion of the BCR and ABL1 genes is present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDK1-mediated phosphorylation, suggesting a cell cycle function for ABL1. ABL1 F317L recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

ABL1 [E255K] Recombinant Human Protein

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. The E255K mutant is a Gleevec resistant form of ABL1.

MATK (Hyl) Recombinant Human Protein

MATK (Hyl) is a non-receptor CSK-type protein tyrosine kinase that is thought to function in signal transduction pathways important in megakaryocyte growth and⁄or differentiation.

BLK Recombinant Human Protein

B-lymphoid tyrosine kinase (BLK) is a non-receptor tyrosine kinase that belongs to the Src family of kinases. BLK plays an important role in B-cell activation.

ABL2 (Arg) Recombinant Human Protein

ABL2 (Arg) is an Abl-related non-receptor protein tyrosine kinase that links cell surface receptors to cell proliferation and⁄or cytoskeletal reorganization.

PEAK1 Recombinant Human Protein (Invitrogen™)

This PEAK1 recombinant human protein (1127–end) was expressed in insect cells. PEAK1 cytoplasmic tyrosine kinase or Pseudopodium-enriched atypical kinase 1 is a protein tyrosine kinase which belongs to NKF3 kinase family and regulates the cytoskeleton and has been shown to be involved in cancer progression. PEAK1 promotes anchorage-independent growth in the MDA-MB-435 human cancer cell line and promotes tumor progression of XPA-1 human pancreatic cancer cells when transplanted into the pancreas of nude mice. PEAK1 is highly expressed in spleen, followed by heart, ovary, brain, lung, kidney, and fetal brain. PEAK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

ABL1 Recombinant Human Protein

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response.

BMX Recombinant Human Protein

Bone marrow tyrosine kinase (BMX) is a non-receptor tyrosine kinase that belongs to the SRC-related TEC subfamily of tyrosine kinases. It is an important regulator of various cellular processes including apoptosis, cell survival, cellular differentiation, cell migration, and transformation.

CSK Recombinant Human Protein

CSK is a tyrosine kinase that has been shown to downregulate the activity of SRC oncoprotein through phosphorylation of its carboxy terminus. Since cell transformation by SRC is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene.

TEC Recombinant Human Protein

TEC is a non-receptor protein-tyrosine kinase containing a pleckstrin homology domain and is highly expressed in many hematopoietic cell lines.

ABL1 [G250E] Recombinant Human Protein

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. The G250E mutant is a Gleevec resistant form of ABL1.

ZAP-70 Recombinant Human Protein

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation.

PTK2 (FAK) Recombinant Human Protein

PTK2 (FAK) is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents.

SYK Recombinant Human Protein

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer.

SRC N1 Recombinant Human Protein

SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRCN1 is a neuronal splice variant of SRC.

TYK2 Recombinant Human Protein

Tyrosine Kinase 2 (TYK2) a member of the JAK kinase family, plays both structural and catalytic roles in type I interferon (IFN) signaling.

ABL1 [T315I] Recombinant Human Protein

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. The T315I mutant is a Gleevec resistant form of ABL1.

PTK6 (BRK) Recombinant Human Protein

Breast tumor kinase (Brk) is a non-receptor protein tyrosine kinase expressed in over 60% of breast carcinoma tissue samples and breast tumor cell lines.

YES1 Recombinant Human Protein

YES1 is a recombinant human protein kinase that is a member of the SRC family of non-receptor tyrosine kinases and is the cellular homolog of the Yamaguchi sarcoma virus oncogene. It is involved in the regulation of cell growth and survival, including roles in apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation.

Active—this protein can be used in kinase activity assays, such as Z’-LYTE® assays, or in a binding assay platform like the LanthaScreen® Eu Kinase Binding Assay (KBA).

Purified—purity is determined by gel electrophoresis and provided in lot-specific certificate of analysis (COA).

GST-tagged—provides animal origin flexibility. When used in the KBA platform, it can be paired with a goat- or rabbit-derived Eu-anti-GST antibody.

FER Recombinant Human Protein

FER is a member of the FPS⁄FES family of non-receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.

ABL1 F317I Recombinant Human Protein (Invitrogen™)

This ABL1 F317I recombinant human protein (27–end) was expressed in insect cells. ABL1 tyrosine kinase is a member of the ABL family and has been implicated in wide range of cellular processes including: cell differentiation, cell division, cell adhesion, and stress response. The purified catalytic domain (27-end) of the ABL1 F317I mutant can be used in a number of different applications, but has been specifically validated for use with our Z’-LYTE™ assay technology to identify novel kinase inhibitors. ABL1 F317I recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

JAK3 Recombinant Human Protein

Janus kinase 3 (JAK3) phosphorylates members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to activation of interleukin receptors.

AXL [R499C] Recombinant Human Protein

AXL, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase which is a member of the TAM (TYRO3/AXL/MER) receptor tyrosine kinase subfamily. AXL represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats and Axl transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. AXL is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Recombinant human AXL R499C (473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

FYN A Recombinant Human Protein

Fyn A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC. Fyn has been shown to phosphorylate Dab1, an intracellular adaptor protein that interacts with amyloid precursor protein (APP) and apoE receptor 2 (apoEr2). The interaction of Fyn and Dab1 regulates the phosphorylation, trafficking, and processing of APP and apoEr2. Fyn expression has been shown to be significantly increased in Chronic Myelogenous Leukemia (CML). Knockdown of Fyn with shRNA slows leukemia cell growth, inhibits clonogenicity, and leads to increased sensitivity to imatinib. Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

FRK (PTK5) Recombinant Human Protein

FRK (PTK5) is a nuclear protein and may function during G1 and S phase of the cell cycle, suppressing growth.

LYN B Recombinant Human Protein

LYN is a SRC family kinase expressed predominantly in hematopoietic cells. Activation of LYN has been shown to lead to imatinib resistance in CML patients. LYNB and the slightly shorter LYNA are the two isoforms of LYN.

TXK Recombinant Human Protein

TXK, along with the close relatives BMS, ITK, and BTK, signal downstream of antigen receptors and other types of receptors.

SRC Recombinant Human Protein

SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth.

SRMS (SRM) Recombinant Human Protein

SRMS (Srm) is a tyrosine protein kinase (SRC sub-family) that may be involved in the proliferation and differentiation of keratinocytes.

JAK2 [V617F] Recombinant Human Protein, JH1 and JH2 Domains

JAK2 is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. This kinase contains both the JH1 (catalytic) and JH2 (pseudokinase) domains of JAK2. The V617F mutation has been shown to be a cause of polycythemia vera.

ABL1 [Y253F] Recombinant Human Protein

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. The Y253F mutant is a Gleevec resistant form of ABL1.

PTK2B (FAK2) Recombinant Human Protein

PTK2B (FAK2) is a cytoplasmic tyrosine kinase involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway.

JAK1 Recombinant Human Protein

Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha⁄beta and -gamma signal transduction pathways.

JAK2 Recombinant Human Protein, JH1 and JH2 Domains

JAK2 is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways and is mutated in polycythemia vera. This kinase contains both the JH1 (catalytic) and JH2 (pseudokinase) domains of JAK2.

TNK2 (ACK) Recominant Human Protein

TNK2 (ACK) (activated Cdc42-associated kinase) is a non-receptor tyrosine kinase that signals downstream of Cdc42. TNK2 is known to be involved in tumorigenesis and metastasis, particularly in prostate cancer.

TNK1 Recombinant Human Protein (Invitrogen™)

This TNK1 recombinant human protein (1–510) was expressed in insect cells. TNK1 tyrosine kinase, non-receptor 1 belongs to the tyrosine protein kinase family and as such is an important regulator of intracellular signal transduction pathways mediating cellular proliferation, survival, and development. TNK1 is highly expressed in fetal tissues and at lower levels in a few adult tissues. TNK1 may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. TNK1 plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. TNK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z’-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

FGR Recombinant Human Protein

FGR is a SRC-related kinase present in hematopoietic cells. FGR signals within multiple pathways including within the integrin pathway and downstream of chemokine and cytokine receptors.

ABL1 [Q252H] Recombinant Human Protein

Human ABL1, internal fragment, amino acids P118-S535 (as in GenBank entry NM_005157.3), Q252H mutant, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

BTK Recombinant Human Protein

Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily of tyrosine kinases. Mutations in the BTK gene have been linked to severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders.

LCK Recombinant Human Protein

LCK is a SRC-related kinase family expressed in lymphocytes.

JAK2 Recombinant Human Protein

Janus kinase 2 (JAK2) is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways and is mutated in polycythemia vera. This kinase contains the catalytic domain (JH1) of JAK2.

ABL1 [M351T] Recombinant Human Protein

Human ABL1, internal fragment, amino acids P118-S535 (as in GenBank entry NM_005157.3), M351T mutant, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

FYN Recombinant Human Protein

FYN is a SRC-related kinase expressed in brain, skeletal muscle and adipose tissues. Fyn functions in a large number of pathways and has been shown to play a role in memory.

FES (FPS) Recombinant Human Protein

FES (FPS) is a tyrosine protein kinase that is required for maintenance of cellular transformation. Its chromosomal location is linked to a specific translocation event identified in patients with acute promyelocytic leukemia, and it is also involved in normal hematopoiesis.

ABL1 [H396P] Recombinant Human Protein

Human ABL1, internal fragment, amino acids P118-S535 (as in GenBank entry NM_005157.3), H396P mutant, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay