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BSA, acetylated (20 mg/mL) (Invitrogen™)

Ambion® Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential. Six tubes of 25 mg are provided, at a concentration of 20 mg/mL. The acetylation process inactivates any trace nuclease activity that might be present. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is routinely added to restriction digests. The molecular weight is ~66 kD. Acetylated BSA is rigorously tested for contaminating nonspecific endonuclease, exonuclease, protease, and RNase activity.

UltraPure™ BSA (50 mg/mL) (Invitrogen™)

UltraPure BSA is a non-acetylated bovine serum albumin (BSA) pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. BSA has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. In northern, Southern, and dot blot hybridizations, BSA is also used as a blocking agent. It is recommended in buffers for nick translation, polymerase reactions, and ligations. BSA is also a common additive for PCR amplifications, footprinting, and gel shift assays. In restriction digests, BSA has been shown to enhance enzyme activity. The molecular weight is 68 kD. UltraPure BSA is rigorously tested for DNase, RNase, endonuclease, and protease activity.

Human BDNF Recombinant Protein, eBioscience™ (Invitrogen™)

Human BDNF Recombinant Protein for Ctrl, FN

Annexin V Recombinant Protein, eBioscience™ (Invitrogen™)

Annexin V Recombinant Protein for Flow, Ctrl

Pierce™ 3x DYKDDDDK Peptide (Thermo Scientific™)

Pierce 3x DYKDDDDK Peptide is a lyophilized peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK). It has a molecular weight of 2861.9 g/mol. For use, reconstitute to 1.5 mg/mL in PBS.

This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH. Note that Pierce 3x DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm. However, the peptide can be removed from the eluate by desalting (e.g., with Zeba Spin Desalting Columns, Cat. No. 89891).

HA Synthetic Peptide (Thermo Scientific™)

Lyophilized peptide with the sequence N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C. Molecular weight: 1102.2 g/mol

26184 can be used to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin. Use 4 X 1 column volumes of 1 mg/ml HA peptide solution to elute HA-tagged proteins. Elution yields will increase by incubating the peptide on the column for 10-15 minutes at 30-37ºC for each elution fraction.

Reconstitute to 1 mg/ml using TBS or distilled water.

The HA peptide, NH2-YPYDVPDYA-COOH, is a synthetic peptide used to competitively elute fusion proteins containing the influenza hemagglutinin (HA) tag bound to immobilized anti-HA affinity resins.

HIV1 nef Recombinant Protein (Invitrogen™)

HIV1 nef Recombinant Protein for Western Blot, ELISA, Ctrl

Annexin V Recombinant Protein, APC, eBioscience™ (Invitrogen™)

Annexin V Recombinant Protein for Flow, Ctrl

GFP Recombinant Aequorea victoria Protein, N-His Tag

Green Fluorescent Protein (GFP) recombinant Aequorea victoria protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein structure analysis and in cell biology research applications.

This recombinant protein is expressed from a DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238) fused to a polyhistidine tag at the N-terminus.

N-terminal Sequence Analysis: Glu 99.

Activity:
1. Mesured by emission analysis.
2. Measure excitation and emission spectra of GFP at wavelengths of 395 nm and 510 nm respectively.

Formulation: Lyophilized from sterile PBS, pH 7.5.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

Background
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of β-sheet with an α-helix inside and short helical segments on the ends of the cylinder. Its ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has long been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.

You may also be interested in the following GFP products:
Vectors
Primary Antibodies
Cellular Structure Probes

LYVE-1 Recombinant Protein (Invitrogen™)

Human LYVE1 Recombinant Protein for Ctrl

Protein A, Alexa Fluor™ 488 conjugate (Invitrogen™)

Our Alexa Fluor 488 protein A conjugate binds to the Fc portion of various immunoglobulins from several different species. This exceptionally bright, green-fluorescent probe (absorption/emission maxima ~495/519 nm) can be used to visualize antibodies in a manner analogous to a labeled species-specific secondary antibody.

PEPotec™ SRM Custom Peptide Libraries (Thermo Scientific™)

Thermo Scientific PEPotec SRM Peptide Libraries are fully synthetic custom libraries for the development of mid- to high-throughput absolute quantitation with selective-reaction monitoring (SRM) or multiple-reaction monitoring (MRM) assays. The PEPotec SRM Peptide Libraries offer great convenience and flexibility for the development of quantitative mass spectroscopy (MS) with many customizable options. The standard service supplies a suspension of at least 0.1 mg of each crude peptide housed in individual tubes in a 96-well plate format with either arginine (R) or lysine (K) as the C-terminal amino acid (other C-terminal amino acids are available). Three quality control grades are available, and optional services and peptide modifications are offered to give you the peptide libraries that fit your experimental needs.

Learn more about our PEPotec SRM Custom Peptide Libraries ›

Highlights:
Traceable—peptides are provided in individual 2-D barcoded tubes in 96-tube plates
Customized—libraries available in various grades with optional services available
Convenient—standard libraries are delivered solubilized in 0.1% trifluoroacetic acid (TFA) in 50% (v/v) acetonitrile/water
Flexible—extensive list of available modifications

Applications:
• Mid- to high-throughput development of SRM and MRM assays
• MS workflows with relative and absolute quantitation strategies

Includes:
• Fully synthetic, crude (as synthesized) peptides
• Multiple grades of QC analysis and optional services and modifications
• Provided in individual Thermo Scientific Matrix 96-tube plates

For a quote or more information, email us at: peps@thermofisher.com.
For Europe, Middle East, and Africa, please email us at: europeservicespeptides@thermofisher.com.

Pierce™ Recombinant GFP Protein (Thermo Scientific™)

Recombinant GFP (Green Fluorescent Protein) is intended as a convenient positive control for the in vitro synthesis of GFP using the Thermo Scientific 1-Step Human Coupled IVT Kit.

More Product Data
Protocol for in vitro protein expression using mRNA templates
Glycoprotein expression in a human IVT system

HIV1 nef Recombinant Protein (Invitrogen™)

HIV1 nef Recombinant Protein for Western Blot, ELISA, Ctrl

PARP1 Recombinant Human Protein, His Tag

Poly (Adp-Ribose) Polymerase 1 (PARP1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from The amino acids corresponding to the full length of human PARP1 (NP_001609.2) (Met 1-Trp 1014) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human PARP1 at 10 µg/ml (100 µl/well) can bind to biotinylated human HSP70. The EC50 of biotinylated human HSP70 is 0.035 µg/ml.

Formulation: 50 mM Tris, 100 mM NaCl, pH 8, 2 mM EDTA, 2 mM PMSF, 0.5 mM TCEP, 10% glycerol.

Reconstitution: No reconstitution needed. Store at -80°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.