Shop All Gateway Cloning Enzymes

Gateway™ LR Clonase™ Enzyme mix (Invitrogen™)

Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format. LR Clonase II Plus enzyme mix is the latest version that offers the highest recombination efficiency available(Table 1), and is specifically optimized for both single and multiple fragment cloning (Table 1). LR Clonase II Plus enzyme mix is provided in an optimized single mix of enzyme and reaction buffer, ensuring enzyme stability and easy of use with few pippetting steps for both single and multiple fragment cloning. Our LR Clonase II enzyme mix is also available in a single mix format, while the original LR Clonase® enzyme is the enzyme and buffer in separate tubes

Gateway™ LR Clonase™ II Enzyme mix (Invitrogen™)

Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone. Gateway® LR Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set up with fewer pipetting steps.

LR Clonase II for BaculoDirect™ Kits (Invitrogen™)

The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway® Technology with baculovirus expression makes the BaculoDirect™ System the fastest and easiest method for production of recombinant baculovirus.

Gateway™ BP Clonase™ Enzyme Mix (Invitrogen™)

Gateway BP Clonase® enzyme contains both Int (Integrase) and IHF (Integration Host Factor) proteins that catalyze the in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and a Donor vector (containing attP sites) to generate Entry clones. BP Clonase® II enzyme format is a single mix of both enzyme and buffer to ensure enzyme stability and enable convenient 10µl reaction set up with fewer pipetting steps, which both maximize recombination efficiency and minimize pippetting. The original format of BP Clonase enzyme is the stand alone enzyme blend and reaction buffer provided in separate tubes.

LR Clonase™ II Plus enzyme (Invitrogen™)

Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format. LR Clonase II Plus enzyme mix is the latest version that offers the highest recombination efficiency available(Table 1), and is specifically optimized for both single and multiple fragment cloning (Table 1). LR Clonase II Plus enzyme mix is provided in an optimized single mix of enzyme and reaction buffer, ensuring enzyme stability and easy of use with few pippetting steps for both single and multiple fragment cloning. Our LR Clonase II enzyme mix is also available in a single mix format, while the original LR Clonase® enzyme is the enzyme and buffer in separate tubes

Gateway™ BP Clonase™ II Enzyme mix (Invitrogen™)

Gateway® BP Clonase® II enzyme mix catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing attB sites) and a donor vector (containing attP sites) to generate entry clones. Gateway® BP Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set up with fewer pipetting steps.