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AmpliTaq™ DNA Polymerase with Buffer I (Applied Biosystems™)

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:
  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it reliable for PCR applications
  • It is QC-tested to guarantee reproducible results

Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

AmpliTaq™ DNA Polymerase with Buffer II (Applied Biosystems™)

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it good for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    Phusion High-Fidelity PCR Master Mix with HF Buffer (Thermo Scientific™)

    Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

    Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

    Highlights

    • High fidelity (50X Taq)
    • Fast PCR due to short extension times (15-30 s/kb)
    • Robust performance, minimal optimization needed

    Applications

    • High-fidelity PCR
    • Amplification of difficult (GC-rich) templates
    • Cloning
    • Template generation for sequencing
    • Long-range PCR (up to 20 kb)
    • Mutagenesis
    • High throughput PCR

    Using Phusion DNA Polymerases
    Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

    AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2 (Applied Biosystems™)

    AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

    • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
    • Time-released thermal activation improves sensitivity in low copy number amplifications
    • Successful multiplex PCR saves time and reagents
    • Includes GeneAmp® 10X PCR Buffer II with MgCl2

    Hot-start activation
    The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

    Higher specificity, higher yield
    AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

    AmpliTaq Gold® 360 DNA Polymerase for superior performance
    Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

    AmpliTaq Gold™ DNA Polymerase with Buffer I (Applied Biosystems™)

    AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

    • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
    • Time-released thermal activation improves sensitivity in low copy number amplifications
    • Successful multiplex PCR saves time and reagents
    • Includes GeneAmp® 10X PCR Buffer I containing 15 mM MgCl2

    Hot-start activation
    The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

    Higher specificity, higher yield
    AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

    AmpliTaq Gold® 360 DNA Polymerase for superior performance
    Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

    GeneAmp™ Fast PCR Master Mix (2X) (Applied Biosystems™)

    The GeneAmp® Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti® 96-Well Thermal Cycler. Features of this master mix:

    • Set up reactions quickly, using premixed reagents
    • Detect a single copy of a gene in as little as 10 ng of DNA
    • Get reproducible, specific, and sensitive results

    Easy to use
    Pre-mixed, hot-start chemistries enable you to set up your experiments quickly. And by generating greater than 90% "A" overhangs—it makes cloning easier too.

    Detect a single copy in 10 ng of DNA
    Use the GeneAmp® Fast PCR Master Mix to detect a single copy of a gene in 10 ng of human genomic DNA or as little as 10 copies of an HIV target.

    Part of the Applied Biosystems® Fast PCR System
    The Applied Biosystems® Fast PCR System, consisting of the premixed GeneAmp® Fast PCR Master Mix, specially designed microplates and tubes, and the Veriti® 96-Well Thermal Cycler (0.1 mL or 0.2 mL), considerably reduces PCR reaction times.

    Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific™)

    Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

    Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

    Highlights

    • High fidelity (25X Taq)
    • Fast PCR due to short extension times (15-30 s/kb)
    • Robust performance, minimal optimization needed

    Applications

    • Amplification of difficult (GC-rich) templates
    • High-fidelity PCR
    • Cloning
    • Template generation for sequencing
    • Long-range PCR (up to 20 kb)
    • Mutagenesis
    • High throughput PCR

    Using Phusion DNA Polymerases
    Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

    Platinum™ Tfi Exo(-) DNA Polymerase (Invitrogen™)

    Platinum® Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup. Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic "hot start" for increased specificity, sensitivity, and yield. Tfi Exo(-) DNA Polymerase is purified from E. coli. expressing cloned mutants of the Thermus filiformis DNA polymerase gene. This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness. Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.

    Applications
    Platinum® Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum® Taq DNA Polymerase without modification. PCR performance is comparable to that of Platinum® Taq in yield, specificity, fidelity, and robustness. Like Platinum® Taq, Platinum® Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.

    Phire Green Hot Start II PCR Master Mix (Thermo Scientific™)

    Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction. The master mix also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

    Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

    Highlights

    Quick hot start—No reactivation step
    Fast enzyme—Amplify four times faster than with hot start Taq
    Robust—Minimal reaction optimization due to high inhibitor tolerance
    High yields—Abundant products due to high efficiency
    Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq
    Direct loading on gel

    Applications

    • Hot Start PCR
    • Routine PCR
    • Non-high fidelity PCR
    • Fast PCR
    • High throughput PCR
    • Genotyping

    Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.

    AmpliTaq™ DNA Polymerase, LD (Low DNA) with Buffer I (Applied Biosystems™)

    Applied Biosystems® AmpliTaq® DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq® DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.

    Manufacturing process results in highly purified enzyme with lower levels of bacterial DNA

    • Minimizes non-specific PCR products when amplifying bacterial targets
    • Highly purified enzyme making it particularly useful for low copy number amplifications

    High-Quality
    This highly purified enzyme preparation ensures that non-target, false-positive PCR products will be effectively minimized when amplifying bacterial sequences. This is especially useful for low-copy-number PCR amplifications. The enzyme is quality-control tested to verify that fewer than 10 copies of bacterial 16S ribosomal RNA gene sequences are present in a standard 2.5 unit aliquot. AmpliTaq® DNA Polymerase, LD is supplied with GeneAmp® 10X PCR Buffer I. It is also available with GeneAmp® 10X PCR Buffer II and MgCl2 Solution.

    Note:
    See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.

    Phusion™ High-Fidelity DNA Polymerase (2 U/µL) (Thermo Scientific™)

    Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

    Highlights

    • High fidelity (52X Taq)
    • Fast PCR due to short extension times (15-30 s/kb)
    • Robust performance, minimal optimization needed
    • High yields of PCR products with minimal enzyme amounts
    • Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

    Applications

    • High-fidelity PCR
    • Cloning
    • Template generation for sequencing
    • Amplification of difficult (GC-rich) templates
    • Long-range PCR (up to 20 kb)
    • Mutagenesis
    • High throughput PCR
    • Microarray

    Using Phusion DNA Polymerases
    Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

    DreamTaq™ Hot Start DNA Polymerase (Thermo Scientific™)

    Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

    DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq buffer containing magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available with pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

    DreamTaq Hot Start DNA Polymerase is formulated to enhance productivity through:
    • Better reaction outcomes
       --Higher yield of target amplicons from low template amounts
       --Increased reaction specificity due to reliable hot-start technology
       --Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
    • Enhanced convenience
       --Room temperature reaction set-up
       --Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer

    Applications:
    • Routine PCR
    • Colony PCR
    • Genotyping
    • RT-PCR
    • Generation of PCR products for TA cloning

    More DreamTaq Hot Start DNA Polymerase products >

    Phusion™ Hot Start II DNA Polymerase (2 U/µL) (Thermo Scientific™)

    Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

    Using Phusion Hot Start II High-Fidelity DNA Polymerase, amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature.
    The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures so it does not require a separate activation step in PCR protocols.

    Highlights

    • Reaction set up at room temperature
    • No non-specific amplification and primer degradation during reaction set up
    • Zero-time reactivation due to unique hot start technology
    • High fidelity (52X Taq)
    • Fast PCR due to short extension times (15-30 s/kb)
    • Robust reactions, minimal optimization needed
    • Increased product yields with minimal enzyme amounts
    • Available as a Green buffer format permitting direct loading on gels (F-537S or F-537L)

    Applications

    • High-fidelity PCR
    • High throughput
    • Amplification of difficult (GC-rich) templates
    • Template generation for sequencing
    • Multiplex PCR
    • Long-range PCR
    • Cloning
    • Mutagenesis
    • Microarray

    Using Phusion DNA Polymerases
    Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

    Platinum™ Taq DNA Polymerase, DNA-free (Invitrogen™)

    Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the highest sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.

    DNA-free Platinum Taq DNA Polymerase offers:
    • Certified low-DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
    • Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
    • Same performance as Platinum Taq DNA Polymerase manufactured using conventional process

    Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic “hot start” provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity.

    Use Platinum Taq DNA Polymerase, DNA-free, for the amplification of DNA from complex genomic and viral templates, as well as in RT-PCR. It is the perfect choice for PCR assays where higher sensitivity and specificity are needed to minimize ambiguous or false-positive results. Find out more at www.thermofisher.com/dna-free.

    AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 (Applied Biosystems™)

    AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

    • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
    • Time-released thermal activation improves sensitivity in low copy number amplifications
    • Successful multiplex PCR saves time and reagents
    • Includes GeneAmp® 10X PCR Gold Buffer with MgCl2

    Hot-start activation
    The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

    Higher specificity, higher yield
    AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

    GeneAmp® 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp® 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold® DNA Polymerase.

    AmpliTaq Gold® 360 DNA Polymerase for superior performance
    Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.