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Platinum™ SuperFi™ Green DNA Polymerase (Invitrogen™)

Invitrogen™ Platinum™ SuperFi™ Green DNA Polymerase provides Platinum™ SuperFi™ DNA Polymerase and a 5X SuperFi™ Green Buffer. The SuperFi Green Buffer contains a density reagent and two tracking dyes for convenient direct gel loading of PCR products.

Benefits of Platinum SuperFi DNA Polymerase include:
• Exceptional >100X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult-to-amplify targets including those of sub-optimal purity or with ˃65% GC content
• Convenient workflow with room temperature reaction setup and 24 h bench top stability of pre-assembled reactions

Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >100X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Applications of Platinum SuperFi DNA Polymerase

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR

Using Platinum SuperFi Green DNA Polymerase
Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use the Tm calculator on our website: www.thermofisher.com/tmcalculator.

Platinum SuperFi Green DNA Polymerase is supplied with a separate vial of SuperFi™ GC Enhancer designed for difficult and GC-rich templates (˃65% GC). The SuperFi Green buffer that is also included contains a density reagent and two electrophoresis tracking dyes allowing direct loading of PCR products on a gel. The dyes in the green buffer do not interfere with PCR performance and are compatible with downstream applications including DNA sequencing, ligation, and restriction digestion.

Platinum hot-start technology efficiently inhibits DNA polymerase activity at ambient temperatures, allowing convenient reaction setup at room temperature. In addition, pre-assembled PCR reactions may be stored at room temperature for up to 24h prior to the PCR.

More Platinum SuperFi DNA Polymerase products ›

Platinum™ Taq DNA Polymerase (Invitrogen™)

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable "hot start" thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The "hot start" property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

Using Platinum Taq DNA Polymerase
The hot-start property of Platinum Taq DNA Polymerase allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.

Note: For superior PCR performance, we recommend next-generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

AmpliTaq™ DNA Polymerase with Buffer II (Applied Biosystems™)

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it good for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    Platinum™ Taq DNA Polymerase High Fidelity (Invitrogen™)

    Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. Features of Platinum® Taq DNA Polymerase, High Fidelity:

    Fidelity—greater than six times higher fidelity than Taq DNA polymerase
    Amplicon size—amplification of fragments up to 15 kb (see figure)
    Convenience—room temperature reaction assembly
    Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR

    Unit definition
    One unit of Platinum® Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

    Platinum™ GenoType Tsp DNA Polymerase (Invitrogen™)

    Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase:

    • Exhibits minimal non-templated nucleotide addition to PCR products
    • Lacks both 5´ and 3´ exonuclease activity
    • Amplifies fragments up to 500 bp

    Application
    Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.

    Unit definition
    One unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.

    AmpliTaq™ DNA Polymerase, LD (Low DNA) with Buffer I (Applied Biosystems™)

    Applied Biosystems® AmpliTaq® DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq® DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.

    Manufacturing process results in highly purified enzyme with lower levels of bacterial DNA

    • Minimizes non-specific PCR products when amplifying bacterial targets
    • Highly purified enzyme making it particularly useful for low copy number amplifications

    High-Quality
    This highly purified enzyme preparation ensures that non-target, false-positive PCR products will be effectively minimized when amplifying bacterial sequences. This is especially useful for low-copy-number PCR amplifications. The enzyme is quality-control tested to verify that fewer than 10 copies of bacterial 16S ribosomal RNA gene sequences are present in a standard 2.5 unit aliquot. AmpliTaq® DNA Polymerase, LD is supplied with GeneAmp® 10X PCR Buffer I. It is also available with GeneAmp® 10X PCR Buffer II and MgCl2 Solution.

    Note:
    See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.

    AmpliTaq Gold™ 360 DNA Polymerase (Applied Biosystems™)

    The Applied Biosystems® AmpliTaq Gold® 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
    * Optimized for the broadest range of targets—from everyday to challenging
    * Unmatched sensitivity, specificity, and yield
    * Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
    * Achieves the highest quality sequencing data

    Optimized for Easy and Challenging Targets

    Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).
    As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

    Optimized for Hot-Start PCR

    AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.
    When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2. The exquisite specificity allows easier multiplexing and allelic discrimination.
    The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A & B. The exquisite specificity allows easier multiplexing and allelic discrimination.

    Superior Sensitivity and Amplification Length

    Compared to the original AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.
    AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 3), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 Kb) sequences. Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA.
     

    Note:

    See user's manual or package insert for limited label license, and trademark information.
    For Research Use Only. Not for use in diagnostics procedures.

    DreamTaq Green DNA Polymerase (5 U/µL) (Thermo Scientific™)

    Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.

    The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications requiring PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq Buffer (#B65) or purifying the PCR product before analysis. The 10X DreamTaq Green Buffer is optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM.

    Highlights

    • Direct loading of PCR product on gel
    • High yields and high sensitivity of PCR
    • Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
    • Robust amplification with minimal optimization
    • Incorporates modified nucleotides, but does not incorporate dUTP

    Applications

    • Routine PCR amplification of DNA fragments up to 6 kb
    • Genotyping
    • High throughput PCR

    AmpliTaq™ DNA Polymerase, LD (Low DNA) with Buffer II (Applied Biosystems™)

    Applied Biosystems® AmpliTaq® DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq® DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.

    Manufacturing process results in highly purified enzyme with lower levels of bacterial DNA

    • Minimizes non-specific PCR products when amplifying bacterial targets
    • Highly purified enzyme making it particularly useful for low copy number amplifications

    High-Quality
    This highly purified enzyme preparation ensures that non-target, false-positive PCR products will be effectively minimized when amplifying bacterial sequences. This is especially useful for low-copy-number PCR amplifications. The enzyme is quality-control tested to verify that fewer than 10 copies of bacterial 16S ribosomal RNA gene sequences are present in a standard 2.5 unit aliquot. AmpliTaq® DNA Polymerase, LD is supplied with GeneAmp® 10X PCR Buffer II and MgCl2 Solution. It is also available with GeneAmp® 10X PCR Buffer I.

    Note:
    See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.

    Taq DNA Polymerase, recombinant (Invitrogen™)

    Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.

    With our Taq DNA Polymerase, you get:

    • Your choice of recombinant or native enzyme
    • Amplification of PCR products up to 5 kb in size
    • An enzyme that is licensed and qualified for PCR

    Applications
    Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.

    Source
    Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.

    Unit definition
    One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    AmpliTaq Gold™ 360 Master Mix (Applied Biosystems™)

    AmpliTaq Gold® 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package—all components are premixed and premeasured. AmpliTaq Gold® 360 Master Mix was designed for 360° coverage of a full range of targets. The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers. Designed for convenience, the AmpliTaq Gold® 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold® 360 DNA Polymerase but it also contains the world-class 360 GC Enhancer which can be added optionally for high GC–content templates. With the wide template coverage, optimization of PCR reaction conditions, is virtually eliminated with the use of AmpliTaq Gold® 360 Master Mix. Key features:

    • Optimized for the broadest range of targets—from everyday to challenging
    • Unmatched sensitivity, specificity, and yield
    • Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
    • Achieves the highest-quality sequencing data
    • Easy-to-use, premixed master mix

    Optimized for easy and challenging targets
    Challenging targets include AT-rich, GC-rich, primer-dimer–forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (see figure). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (see table).

    Optimized for automated, hot-start PCR
    AmpliTaq Gold® 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR. When AmpliTaq Gold® 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured. The amount of AmpliTaq Gold® 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets (see figure). The extreme specificity allows easier multiplexing and allelic discrimination.

    Amplify low-copy amplicons and long targets
    AmpliTaq Gold® 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold® 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences. The figure shown demonstrates robust PCR amplification of long human and plasmid DNA.

    AmpliTaq™ DNA Polymerase with Buffer I (Applied Biosystems™)

    AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

    Features of this enzyme:
    • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
    • Its thermal activity profile makes it reliable for PCR applications
    • It is QC-tested to guarantee reproducible results

    Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    Collibri™ Library Amplification Master Mix with Primer Mix (Invitrogen™)

    Invitrogen Collibri Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries. The master mix includes Platinum SuperFi DNA Polymerase in combination with a proprietary reaction buffer containing all necessary components for efficient library amplification with minimal bias and superior accuracy.

    Feature include:
    High fidelity—minimal error rates similar to PCR-free methods
    High efficiency—high yields from minute input amounts
    Minimal bias—uniform amplification regardless of GC content

    This Collibri Library Amplification Master Mix is supplied with a 10X Primer Mix that targets the P5 and P7 regions of Illumina adapters. The master mix is supplemented with an inert blue dye, while the primer mix contains a yellow dye. Mixing both components in a PCR reaction turns the final PCR solution green. This provides a visual aid when pipetting and helps decrease the risk of pipetting errors during reaction setup.

    Platinum SuperFi DNA Polymerase has both 5’ to 3’ polymerase and 3’ to 5’ exonuclease (proofreading) activities, but lacks 5’ to 3’ exonuclease activity. Exceptionally strong proofreading activity ensures amplification of NGS libraries with supreme sequence accuracy, producing NGS error rates similar to PCR-free methods.

    The proprietary reaction buffer contains all necessary PCR components and additives to enable efficient and uniform library amplification regardless of GC content, helping improve coverage across GC- and AT-rich sequences and other complex regions. High amplification efficiency enables the use of fewer cycles to achieve the desired yield.

    Platinum hot-start technology inhibits DNA polymerase activity at ambient temperatures, allowing room temperature reaction setup and storage of pre-assembled PCR reactions for up to 24 hours prior to the PCR.

    Platinum™ SuperFi™ PCR Master Mix (Invitrogen™)

    Invitrogen™ Platinum™ SuperFi™ PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.

    Benefits of Platinum SuperFi PCR Master Mix include:
    • Exceptional >100X Taq fidelity
    • High specificity and increased yields with Platinum hot-start technology
    • Robust amplification of difficult-to-amplify targets including those of sub-optimal purity or with ˃65% GC content
    • Convenient workflow with room temperature reaction setup and 24 h bench top stability of pre-assembled reactions

    Platinum SuperFi PCR Master Mix retains all the features of Platinum™ SuperFi™ DNA Polymerase, which is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >100X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

    Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

    Applications of Platinum SuperFi DNA Polymerase

    • High-fidelity PCR
    • Cloning and sub-cloning
    • Site-directed mutagenesis
    • Amplification of GC-rich templates
    • Template generation for sequencing
    • High-throughput PCR
    • Amplification of samples with suboptimal purity
    • Long PCR
    • Fast PCR

    Using Platinum SuperFi PCR Master Mix
    Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use the Tm calculator on our website: www.thermofisher.com/tmcalculator.

    Platinum SuperFi PCR Master Mix is supplied with a separate vial of SuperFi™ GC Enhancer designed for difficult and GC-rich templates (˃65% GC).

    Platinum hot-start technology efficiently inhibits DNA polymerase activity at ambient temperatures, allowing convenient reaction setup at room temperature. In addition, pre-assembled PCR reactions may be stored at room temperature for up to 24h prior to the PCR.

    More Platinum SuperFi DNA Polymerase products ›

    Platinum™ SuperFi™ Green PCR Master Mix (Invitrogen™)

    Invitrogen™ Platinum™ SuperFi™ Green PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup. SuperFi Green master mix also contains a density reagent and two tracking dyes for convenient direct gel loading of PCR products.

    Benefits of Platinum SuperFi Green PCR Master Mix include:
    • Exceptional >100X Taq fidelity
    • High specificity and increased yields with Platinum hot-start technology
    • Robust amplification of difficult-to-amplify targets including those of sub-optimal purity or with ˃65% GC content
    • Convenient workflow with room temperature reaction setup and 24 h bench top stability of pre-assembled reactions

    Platinum SuperFi Green PCR Master Mix retains all the features of Platinum™ SuperFi™ DNA Polymerase, which is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >100X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

    Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

    Applications of Platinum SuperFi DNA Polymerase

    • High-fidelity PCR
    • Cloning and sub-cloning
    • Site-directed mutagenesis
    • Amplification of GC-rich templates
    • Template generation for sequencing
    • High-throughput PCR
    • Amplification of samples with suboptimal purity
    • Long PCR
    • Fast PCR

    Using Platinum SuperFi Green PCR Master Mix
    Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use the Tm calculator on our website: www.thermofisher.com/tmcalculator.

    Platinum SuperFi Green Master Mix includes a density reagent and two electrophoresis tracking dyes allowing direct loading of PCR products on a gel. The dyes do not interfere with PCR performance and are compatible with downstream applications including DNA sequencing, ligation, and restriction digestion. SuperFi Green Master Mix is supplied with a separate vial of SuperFi™ GC Enhancer designed for difficult and GC-rich templates (˃65% GC).

    Platinum hot-start technology efficiently inhibits DNA polymerase activity at ambient temperatures, allowing convenient reaction setup at room temperature. In addition, pre-assembled PCR reactions may be stored at room temperature for up to 24h prior to the PCR.

    More Platinum SuperFi DNA Polymerase products ›