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Phusion High-Fidelity PCR Master Mix with HF Buffer (Thermo Scientific™)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

• High fidelity (50X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed

Applications

• High-fidelity PCR
• Amplification of difficult (GC-rich) templates
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific™)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

• High fidelity (25X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed

Applications

• Amplification of difficult (GC-rich) templates
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

True Allele™ PCR Premix (Applied Biosystems™)

Applied Biosystems® True Allele® PCR Premix is a PCR master mix that contains AmpliTaq Gold® DNA Polymerase, dNTPs, MgCl2, and buffer. It is intended for use with the ABI PRISM® Linkage Mapping Set. True Allele® PCR Premix has been optimized for strong, specific PCR amplification of microsatellite markers.

Accessory Products:
ABI PRISM® Linkage Mapping Set

Note:
See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Platinum™ SuperFi™ PCR Master Mix (Invitrogen™)

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.

Benefits of Platinum SuperFi PCR Master Mix include:
• Exceptional >100X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult-to-amplify targets including those of sub-optimal purity or with ˃65% GC content
• Convenient workflow with room temperature reaction setup and 24 h bench top stability of pre-assembled reactions

Platinum SuperFi PCR Master Mix retains all the features of Platinum™ SuperFi™ DNA Polymerase, which is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >100X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Applications of Platinum SuperFi DNA Polymerase

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR

Using Platinum SuperFi PCR Master Mix
Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use the Tm calculator on our website: www.thermofisher.com/tmcalculator.

Platinum SuperFi PCR Master Mix is supplied with a separate vial of SuperFi™ GC Enhancer designed for difficult and GC-rich templates (˃65% GC).

Platinum hot-start technology efficiently inhibits DNA polymerase activity at ambient temperatures, allowing convenient reaction setup at room temperature. In addition, pre-assembled PCR reactions may be stored at room temperature for up to 24h prior to the PCR.

More Platinum SuperFi DNA Polymerase products ›

Phusion U Hot Start PCR Master Mix (Thermo Scientific™)

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start PCR Master Mix is convenient 2X mix containing Phusion U Hot Start DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.

Highlights

Accuracy—high fidelity DNA polymerase (25x Taq)
Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
Specificity—hot start for reduced nonspecific amplification and primer degradation
Speed —short extension times (15-30 s/kb)

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Phusion U Hot Start DNA Polymerase (Thermo Scientific™)

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start DNA Polymerase is also available in the Master Mix and Green formats. Phusion U Hot Start PCR Master Mix (F-533S and F-533L) is convenient 2X mix designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion U Green format (F-556S and F-556L) is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights

Accuracy—high fidelity DNA polymerase (25x Taq)
Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
Specificity—hot start for reduced nonspecific amplification and primer degradation
Speed —short extension times (15-30 s/kb)

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Platinum™ SuperFi™ Green PCR Master Mix (Invitrogen™)

Invitrogen™ Platinum™ SuperFi™ Green PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup. SuperFi Green master mix also contains a density reagent and two tracking dyes for convenient direct gel loading of PCR products.

Benefits of Platinum SuperFi Green PCR Master Mix include:
• Exceptional >100X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult-to-amplify targets including those of sub-optimal purity or with ˃65% GC content
• Convenient workflow with room temperature reaction setup and 24 h bench top stability of pre-assembled reactions

Platinum SuperFi Green PCR Master Mix retains all the features of Platinum™ SuperFi™ DNA Polymerase, which is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >100X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Applications of Platinum SuperFi DNA Polymerase

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR

Using Platinum SuperFi Green PCR Master Mix
Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use the Tm calculator on our website: www.thermofisher.com/tmcalculator.

Platinum SuperFi Green Master Mix includes a density reagent and two electrophoresis tracking dyes allowing direct loading of PCR products on a gel. The dyes do not interfere with PCR performance and are compatible with downstream applications including DNA sequencing, ligation, and restriction digestion. SuperFi Green Master Mix is supplied with a separate vial of SuperFi™ GC Enhancer designed for difficult and GC-rich templates (˃65% GC).

Platinum hot-start technology efficiently inhibits DNA polymerase activity at ambient temperatures, allowing convenient reaction setup at room temperature. In addition, pre-assembled PCR reactions may be stored at room temperature for up to 24h prior to the PCR.

More Platinum SuperFi DNA Polymerase products ›

Taq DNA Polymerase, recombinant (Invitrogen™)

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.

With our Taq DNA Polymerase, you get:

• Your choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• An enzyme that is licensed and qualified for PCR

Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.

Source
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.

Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

DreamTaq™ Hot Start Green PCR Master Mix (Thermo Scientific™)

Thermo Scientific™ DreamTaq™ Hot Start Green PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq Green buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start Green PCR Master Mix is formulated to enhance productivity through:
• Better reaction outcomes
   --Higher yield of target amplicons from low template amounts
   --Increased reaction specificity due to reliable hot-start technology
   --Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
   --Room temperature reaction set-up
   --Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
   --Simplified workflow and minimized pipetting steps with 2X master mix format
   --Direct loading of PCR products on a gel

Applications:
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning

More DreamTaq Hot Start DNA Polymerase products >

AccuPrime™ SuperMix I (Invitrogen™)

AccuPrime™ SuperMix I is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1).

For accurate PCR performance, AccuPrime™ SuperMix I sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. In addition to a powerful DNA polymerase, AccuPrime™ SuperMix I includes Platinum® anti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR (Figure 1). This unique combination allows for success, even under previously suboptimal conditions.

AccuPrime™ SuperMix I is designed for amplification of genomic DNA amplicons (?200 bp), plasmid DNA, or cDNA templates. Alternatively, AccuPrime™ SuperMix II is designed to amplify a broader range of amplicon sizes up to 4kb.

AmpliTaq™ DNA Polymerase with Buffer I (250 units/tube) (Applied Biosystems™)

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:
  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it reliable for PCR applications
  • It is QC-tested to guarantee reproducible results

Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

AmpliTaq Gold™ 360 DNA Polymerase (Applied Biosystems™)

The Applied Biosystems® AmpliTaq Gold® 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.

* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity, specificity, and yield
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets
Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).

As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Optimized for Hot-Start PCR
AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.
When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2. The exquisite specificity allows easier multiplexing and allelic discrimination.

The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A & B. The exquisite specificity allows easier multiplexing and allelic discrimination.

Superior Sensitivity and Amplification Length
Compared to the original AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.

AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 3), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 Kb) sequences. Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Phusion Green High-Fidelity DNA Polymerase (2 U/µL) (Thermo Scientific™)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

The Phusion Green format is a combination of Phusion High-Fidelity DNA Polymerase with 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The density reagent and dyes do not interfere with excellent performance of Phusion DNA Polymerases and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Phusion U Green Hot Start DNA Polymerase (Thermo Scientific™)

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights

Accuracy—high fidelity DNA polymerase (25x Taq)
Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
Specificity—hot start for reduced nonspecific amplification and primer degradation
Speed —short extension times (15-30 s/kb)
Green format—permits direct loading of PCR products on gels

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

AccuPrime™ Pfx DNA Polymerase (Invitrogen™)

AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum® Pfx alone. With AccuPrime™ Pfx DNA Polymerase you will:

• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimizationApplications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime™ Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.