Shop All PCR Enzymes & Kits

Taq DNA Polymerase, native Invitrogen™

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:

  • Your choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • An enzyme that is licensed and qualified for PCR

    Applications
    Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing

    Source
    Native enzyme is purified from Thermus aquaticus YT1.

    Unit definition
    One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
  • Platinum™ Multiplex PCR Master Mix Applied Biosystems™

    The Platinum® Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR. Designed specifically for endpoint multiplex PCR, it supports easy multiplexing with minimal optimization.
    Multiplexing—Amplify up to 20 amplicons in a single reaction
    Flexibility—Amplify products from 50 bp to 2.5 kb
    Ease of Use—Perform multiplex PCR reactions with minimal optimization

    A High-Specificity, High-Throughput Solution for Endpoint PCR
    The performance of the Platinum® Multiplex PCR Master Mix over a wide range of amplicon sizes permits the amplification of templates from 50 bp to 2.5 kb, greatly enhancing workflow flexibility. Coupled with its 20-plex capability and absence of primer dimers, it not only provides a high-throughput solution but also boasts high specificity through fewer non-specific primer binding events, and hence less reaction and primer waste.

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

    DreamTaq™ Hot Start DNA Polymerase Thermo Scientific™

    Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

    DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq buffer containing magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available with pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

    DreamTaq Hot Start DNA Polymerase is formulated to enhance productivity through:
    • Better reaction outcomes
       --Higher yield of target amplicons from low template amounts
       --Increased reaction specificity due to reliable hot-start technology
       --Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
    • Enhanced convenience
       --Room temperature reaction set-up
       --Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer

    Applications:
    • Routine PCR
    • Colony PCR
    • Genotyping
    • RT-PCR
    • Generation of PCR products for TA cloning

    More DreamTaq Hot Start DNA Polymerase products >

    Platinum™ Direct PCR Universal Master Mix Invitrogen™

    Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for superior performance in direct PCR applications.

    Features of Platinum Direct PCR Universal Master Mix include:
    • Direct DNA amplification from various samples due to engineered, inhibitor-tolerant Platinum II Taq Hot-Start DNA Polymerase
    • Universal primer annealing temperature due to innovative buffer that enables primer annealing at 60°C
    • Superior specificity, sensitivity, and yields due to Platinum hot-start technology

    Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The kit includes optimized reagents to achieve superior results. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments. Samples in the lysis buffer can be stored for later use. Platinum Direct PCR Universal Master Mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

    Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

    Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

    View more information about Platinum Direct PCR Universal Master Mix ›

    AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 Applied Biosystems™

    AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

    • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
    • Time-released thermal activation improves sensitivity in low copy number amplifications
    • Successful multiplex PCR saves time and reagents
    • Includes GeneAmp® 10X PCR Gold Buffer with MgCl2

    Hot-start activation
    The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

    Higher specificity, higher yield
    AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

    GeneAmp® 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp® 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold® DNA Polymerase.

    AmpliTaq Gold® 360 DNA Polymerase for superior performance
    Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

    Platinum™ SuperFi II Green PCR Master Mix Invitrogen™

    Invitrogen Platinum SuperFi II Green PCR Master Mix (2X) contains a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels. Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with innovative SuperFi II Buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

    Features of Platinum SuperFi II Green PCR Master Mix include:
    • Exceptional >300X Taq fidelity
    • Universal primer annealing at 60°C
    • Superior specificity, sensitivity, and yields
    • Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)

    Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction set up at room temperature and provides increased sensitivity and yield.

    Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

    Applications of Platinum SuperFi II DNA Polymerase:
    • High-fidelity PCR
    • Cloning and sub-cloning
    • Site-directed mutagenesis
    • Amplification of GC-rich templates
    • Template generation for sequencing
    • High-throughput PCR
    • Amplification of samples with suboptimal purity
    • Long PCR
    • Fast PCR

    Platinum SuperFi II PCR Master Mix is also available, which is the same master mix without the tracking dyes.

    View more information about Platinum SuperFi II DNA Polymerase products ›

    AmpliTaq™ DNA Polymerase with Buffer II Applied Biosystems™

    AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

    Features of this enzyme:

  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it good for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    DreamTaq™ Hot Start Green DNA Polymerase Thermo Scientific™

    Thermo Scientific™ DreamTaq™ Hot Start Green DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq Green buffer that includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel. The buffer contains magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available without pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

    DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

    DreamTaq Hot Start Green DNA Polymerase is formulated to enhance productivity through:
    • Better reaction outcomes
       --Higher yield of target amplicons from low template amounts
       --Increased reaction specificity due to reliable hot-start technology
       --Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
    • Enhanced convenience
       --Room temperature reaction set-up
       --Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
       --Direct loading of PCR products on a gel with DreamTaq Green buffer

    Applications:
    • Routine PCR
    • Colony PCR
    • Genotyping
    • RT-PCR
    • Generation of PCR products for TA cloning

    More DreamTaq Hot Start DNA Polymerase products >

    Platinum™ GenoType Tsp DNA Polymerase Invitrogen™

    Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase:

    • Exhibits minimal non-templated nucleotide addition to PCR products
    • Lacks both 5´ and 3´ exonuclease activity
    • Amplifies fragments up to 500 bp

    Application
    Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.

    Unit definition
    One unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.

    Collibri™ Library Amplification Master Mix with Primer Mix Invitrogen™

    Invitrogen Collibri Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries. The master mix includes Platinum SuperFi DNA Polymerase in combination with a proprietary reaction buffer containing all necessary components for efficient library amplification with minimal bias and superior accuracy.

    Feature include:
    High fidelity—minimal error rates similar to PCR-free methods
    High efficiency—high yields from minute input amounts
    Minimal bias—uniform amplification regardless of GC content

    This Collibri Library Amplification Master Mix is supplied with a 10X Primer Mix that targets the P5 and P7 regions of Illumina adapters. The master mix is supplemented with an inert blue dye, while the primer mix contains a yellow dye. Mixing both components in a PCR reaction turns the final PCR solution green. This provides a visual aid when pipetting and helps decrease the risk of pipetting errors during reaction setup.

    Platinum SuperFi DNA Polymerase has both 5’ to 3’ polymerase and 3’ to 5’ exonuclease (proofreading) activities, but lacks 5’ to 3’ exonuclease activity. Exceptionally strong proofreading activity ensures amplification of NGS libraries with supreme sequence accuracy, producing NGS error rates similar to PCR-free methods.

    The proprietary reaction buffer contains all necessary PCR components and additives to enable efficient and uniform library amplification regardless of GC content, helping improve coverage across GC- and AT-rich sequences and other complex regions. High amplification efficiency enables the use of fewer cycles to achieve the desired yield.

    Platinum hot-start technology inhibits DNA polymerase activity at ambient temperatures, allowing room temperature reaction setup and storage of pre-assembled PCR reactions for up to 24 hours prior to the PCR.

    Phire Hot Start II PCR Master Mix Thermo Scientific™

    Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction.

    Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

    Highlights

    Quick hot start—No reactivation step
    Fast enzyme—Amplify four times faster than with hot start Taq
    Robust—Minimal reaction optimization due to high inhibitor tolerance
    High yields—Abundant products due to high efficiency
    Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq

    Applications

    • Hot Start PCR
    • Routine PCR
    • Non-high fidelity PCR
    • Fast PCR
    • High throughput PCR
    • Genotyping

    Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.

    AmpliTaq™ DNA Polymerase with Buffer I Applied Biosystems™

    AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

    Features of this enzyme:
    • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
    • Its thermal activity profile makes it reliable for PCR applications
    • It is QC-tested to guarantee reproducible results

    Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    AccuPrime™ Taq DNA Polymerase System Invitrogen™

    The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C. Advantages of AccuPrime™ Taq DNA Polymerase:

    Specificity—control mispriming at every cycle to get single-band PCR products
    Ease of use—minimize reaction optimization and primer set redesign
    Convenience—conveniently assemble reactions at room temperature

    AccuPrime™ buffers
    The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

    Applications
    AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning®, and allele-specific amplifications.

    AmpliTaq Gold™ 360 DNA Polymerase Applied Biosystems™

    The Applied Biosystems® AmpliTaq Gold® 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
    * Optimized for the broadest range of targets—from everyday to challenging
    * Unmatched sensitivity, specificity, and yield
    * Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
    * Achieves the highest quality sequencing data

    Optimized for Easy and Challenging Targets

    Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).
    As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

    Optimized for Hot-Start PCR

    AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.
    When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2. The exquisite specificity allows easier multiplexing and allelic discrimination.
    The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A & B. The exquisite specificity allows easier multiplexing and allelic discrimination.

    Superior Sensitivity and Amplification Length

    Compared to the original AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.
    AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 3), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 Kb) sequences. Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA.
     

    Note:

    See user's manual or package insert for limited label license, and trademark information.
    For Research Use Only. Not for use in diagnostics procedures.

    Phire Green Hot Start II PCR Master Mix Thermo Scientific™

    Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction. The master mix also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

    Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

    Highlights

    Quick hot start—No reactivation step
    Fast enzyme—Amplify four times faster than with hot start Taq
    Robust—Minimal reaction optimization due to high inhibitor tolerance
    High yields—Abundant products due to high efficiency
    Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq
    Direct loading on gel

    Applications

    • Hot Start PCR
    • Routine PCR
    • Non-high fidelity PCR
    • Fast PCR
    • High throughput PCR
    • Genotyping

    Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.
    Results per page
      spinner