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Collibri™ Library Amplification Master Mix with Primer Mix Invitrogen™

Invitrogen Collibri Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries. The master mix includes Platinum SuperFi DNA Polymerase in combination with a proprietary reaction buffer containing all necessary components for efficient library amplification with minimal bias and superior accuracy.

Feature include:
High fidelity—minimal error rates similar to PCR-free methods
High efficiency—high yields from minute input amounts
Minimal bias—uniform amplification regardless of GC content

This Collibri Library Amplification Master Mix is supplied with a 10X Primer Mix that targets the P5 and P7 regions of Illumina adapters. The master mix is supplemented with an inert blue dye, while the primer mix contains a yellow dye. Mixing both components in a PCR reaction turns the final PCR solution green. This provides a visual aid when pipetting and helps decrease the risk of pipetting errors during reaction setup.

Platinum SuperFi DNA Polymerase has both 5’ to 3’ polymerase and 3’ to 5’ exonuclease (proofreading) activities, but lacks 5’ to 3’ exonuclease activity. Exceptionally strong proofreading activity ensures amplification of NGS libraries with supreme sequence accuracy, producing NGS error rates similar to PCR-free methods.

The proprietary reaction buffer contains all necessary PCR components and additives to enable efficient and uniform library amplification regardless of GC content, helping improve coverage across GC- and AT-rich sequences and other complex regions. High amplification efficiency enables the use of fewer cycles to achieve the desired yield.

Platinum hot-start technology inhibits DNA polymerase activity at ambient temperatures, allowing room temperature reaction setup and storage of pre-assembled PCR reactions for up to 24 hours prior to the PCR.

Platinum™ II Hot-Start PCR Master Mix (2X) Invitrogen™

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and leading hot-start technology.

Features of Platinum II Hot-Start PCR Master Mix (2X) include:
Innovative buffer—enables universal annealing temperature by isostabilizing primer-template duplex structures
Engineered Taq DNA polymerase—confers fast cycling and resistance to common inhibitors
Platinum hot-start technology—enables superior specificity, sensitivity, and yields; allows for room temperature reaction setup

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Platinum II Hot-Start PCR Master Mix (2X) is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Use Platinum II Hot-Start PCR Master Mix (2X) for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR, in applications like genotyping, high-throughput PCR, or with samples of suboptimal purity.

For further streamlining of PCR workflow, we offer Platinum II Hot-Start Green PCR Master Mix (2X), which additionally contains a density reagent and two tracking dyes for direct gel loading, for even fewer pipetting steps during PCR product analysis.

Find out more at www.thermofisher.com/platinumiitaq ›

Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each) Thermo Scientific™

This package includes Phusion High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).

Phusion High-Fidelity DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA polymerases set a high standard for high performance PCR. Featuring an error rate 52-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts

Using Phusion DNA polymerases
Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Learn more about all Phusion DNA polymerases ›

dNTP Mix
Thermo Scientific dNTP Mix contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E. coli DNA. Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors.

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

Learn more about all dNTPs formats ›

Phusion™ High-Fidelity DNA Polymerase (2 U/µL) Thermo Scientific™

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Highlights

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo Scientific™

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

• High fidelity (50X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed

Applications

• High-fidelity PCR
• Amplification of difficult (GC-rich) templates
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

l">www.thermofisher.com/tmcalculator.

Phusion Hot Start II DNA Polymerase (2 U/µL) Thermo Scientific™

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Using Phusion Hot Start II High-Fidelity DNA Polymerase, amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature.
The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures so it does not require a separate activation step in PCR protocols.

Highlights

• Reaction set up at room temperature
• No non-specific amplification and primer degradation during reaction set up
• Zero-time reactivation due to unique hot start technology
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust reactions, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Available as a Green buffer format permitting direct loading on gels (F-537S or F-537L)

Applications

• High-fidelity PCR
• High throughput
• Amplification of difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Platinum™ II Hot-Start Green PCR Master Mix (2X)

Invitrogen Platinum II Hot-Start Green PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and leading hot-start technology.

Features of Platinum II Hot-Start Green PCR Master Mix (2X) include:
Innovative buffer—enables universal annealing temperature by isostabilizing primer-template duplex structures
Engineered Taq DNA polymerase—confers fast cycling and resistance to common inhibitors
Platinum hot-start technology—enablessures superior specificity, sensitivity, and yields; allows for room temperature reaction setup
Green PCR buffer—helps reduces pipetting error with direct gel loading

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and may deliver PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start Green PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Platinum II Hot-Start Green PCR Master Mix (2X) is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Use Platinum II Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR, in applications like genotyping, high-throughput PCR, or with samples of suboptimal purity.

Platinum II Hot-Start PCR Master Mix (2X) is also available, which is the same master mix without the tracking dyes.

Find out more at www.thermofisher.com/platinumiitaq ›

Phusion High-Fidelity PCR Master Mix with GC Buffer Thermo Scientific™

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

• High fidelity (25X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed

Applications

• Amplification of difficult (GC-rich) templates
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each) Thermo Scientific™

This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).

Phusion Hot Start II High-Fidelity DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA polymerases set a high standard for high performance PCR. Featuring an error rate 52-fold lower than that of Taq, and 6-fold lower than that of Pfu, they are an excellent choice for cloning and other applications requiring high fidelity. They offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

• High fidelity (52X Taq)
• No non-specific amplification and primer degradation during reaction setup
• Fast PCR due to short extension times (15-30 s/kb)
• Increased product yields with minimal enzyme amounts

When using Phusion Hot Start II High-Fidelity DNA Polymerase, amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature. The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction setup. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II High-Fidelity DNA Polymerase is immediately reactivated at high temperatures, so does not require a separate activation step in PCR protocols.

Using Phusion DNA polymerases
Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Learn more about all Phusion DNA polymerases ›

dNTP Mix
Thermo Scientific dNTP Mix contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E. coli DNA. Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors.

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

Learn more about all dNTPs formats ›

AmpliTaq™ DNA Polymerase with Buffer II Applied Biosystems™

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for PCR and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

  • AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it good for PCR applications
  • It is QC-tested to guarantee reproducible results

  • Reliable and robust PCR
    The thermal activity profile of AmpliTaq DNA Polymerase is reliable for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaqDNA Polymerase is supplied with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

    For superior PCR performance, we recommend DreamTaq DNA Polymerase.

    AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2 Applied Biosystems™

    AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

    • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
    • Time-released thermal activation improves sensitivity in low copy number amplifications
    • Successful multiplex PCR saves time and reagents
    • Includes GeneAmp® 10X PCR Buffer II with MgCl2

    Hot-start activation
    The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

    Higher specificity, higher yield
    AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

    AmpliTaq Gold® 360 DNA Polymerase for superior performance
    Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

    Phusion U Green Multiplex PCR Master Mix Thermo Scientific™

    Thermo Scientific Phusion U Green Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations.

    The Phusion U Green Multiplex PCR Master Mix contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization. The Green format further simplifies the workflow - it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.

    Highlights

    • Specific and efficient multiplex PCR of over 20 targets up to 2.5 kb
    • Excellent results with templates of suboptimal purity due to inhibitor tolerance
    • Fast cycling and direct loading of PCR products on gels

    Applications

    • Genotyping
    • Pathogen detection
    • Food testing
    • Analysis of genetically modified organisms
    • Amplification of microsatellites

    The master mix is based on Phusion U Hot Start DNA Polymerase, a high performance enzyme developed using fusion technology. Similarly to other enzymes from Phusion family, Phusion U DNA Polymerase incorporates more nucleotides per binding event than any other DNA polymerase. This allows achieving high yields of PCR products with shorter extention times and consequently reduced total protocol times. Significantly enhanced processivity also enables successful multiplex PCR on difficult targets such as GC-rich templates or templates of suboptimal purity. Being highly tolerant of many PCR inhibitors, Phusion U Multiplex PCR Master Mixes can even be used with unpurified samples such as blood or serum.

    Specificity of multiplex PCR is increased by Affibody-mediated hot start mechanism. Reversibly bound Affibody ligand inhibits the polymerase at ambient temperatures preventing amplification of nonspecific products and formation of primer-dimers.

    Using Phusion DNA Polymerases
    Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

    Platinum™ SuperFi II DNA Polymerase Invitrogen™

    Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

    Features of Platinum SuperFi II DNA Polymerase include:
    • Exceptional >300X Taq fidelity
    • Universal primer annealing at 60°C
    • Superior specificity, sensitivity, and yields
    • Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)

    Platinum SuperFi II DNA Polymerase is an engineered enzyme high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.

    Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

    Applications of Platinum SuperFi II DNA Polymerase:
    • High-fidelity PCR
    • Cloning and sub-cloning
    • Site-directed mutagenesis
    • Amplification of GC-rich templates
    • Template generation for sequencing
    • High-throughput PCR
    • Amplification of samples with suboptimal purity
    • Long PCR
    • Fast PCR

    For increased convenience, we offer Platinum SuperFi II Master Mix with Platinum SuperFi II DNA Polymerase provided in a ready-to-use mixture along with SuperFi II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. Platinum SuperFi II Green PCR Master Mix is also available, which additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels, further streamlining the PCR workflow from setup to final analysis.

    View more information about Platinum SuperFi II DNA Polymerase products ›

    Phire Plant Direct PCR Master Mix Thermo Scientific™

    Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols and others.

    Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria and yeast. The kit includes a complete set of optimized reagents and detailed protocols making it an ideal choice for amplification of plant DNA.

    Highlights

    • Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps.
    • Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
    • Specially engineered Phire Hot Start II DNA polymerase—extremely short PCR protocol times
    • Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

    Applications

    • Genotyping
    • Transgene detection
    • Knockout analysis
    • Sequencing

    The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

    For optimal results start by accurately calculating your Tm with our Tm calculator.

    Phusion Green High-Fidelity DNA Polymerase (2 U/µL) Thermo Scientific™

    Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

    The Phusion Green format is a combination of Phusion High-Fidelity DNA Polymerase with 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The density reagent and dyes do not interfere with excellent performance of Phusion DNA Polymerases and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

    Highlights

    • High fidelity (52X Taq)
    • Fast PCR due to short extension times (15-30 s/kb)
    • Robust performance, minimal optimization needed
    • High yields of PCR products with minimal enzyme amounts
    • Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

    Applications

    • High-fidelity PCR
    • Cloning
    • Template generation for sequencing
    • Amplification of difficult (GC-rich) templates
    • Long-range PCR (up to 20 kb)
    • Mutagenesis
    • High throughput PCR
    • Microarray

    Using Phusion DNA Polymerases
    Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

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