Shop All Enzyme Inhibitors

SUPERase•In™ RNase Inhibitor (20 U/μL) Invitrogen™

SUPERase•In RNase Inhibitor is a protein-based inhibitor of non-human origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1.

SUPERase•In RNase Inhibitor can be used in any application where RNase contamination could be problematic. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERase•In is the most effective RNase inhibitor available, providing a higher level of protection against degradation.

SUPERase•In does not interfere with other enzymes such as RNA polymerases, reverse transcriptase, or Taq DNA polymerase. Additionally, SUPERase•In is active up to 65°C and over a pH range of 5.5 to 8.5.

If you are plannning to use this product with standard antibody purification methods, please contact our technical support to discuss experimental design.

Unit definition
SUPERase•In RNase Inhibitor at 1 U/µL will block the degradation of 0.1 µg/µL labeled RNA by 2.5 pg/µL of RNase A, 2.5 pg/µL of RNase I, and 0.0075 U/µL of RNase T1, for 4 hours at 37°C, in 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM EDTA. Analysis is by denaturing PAGE. SUPERase•In is currently the only ribonuclease inhibitor for which the unit activity is defined by such a functional assay.

Pierce™ Protease Inhibitor Mini Tablets, EDTA-free Thermo Scientific™

Thermo Scientific Pierce Protease Inhibitor Mini Tablets, EDTA-free, contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Features of EDTA-free Protease Inhibitor Mini Tablets:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays
EDTA-free—formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations)

Pierce Protease Inhibitor Mini Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A. Stable for storage at 4°C for up to 12 months, each mini tablet is sufficient for 10 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.

To use Pierce Protease Inhibitor Mini Tablets, simply dissolve 1 tablet in 10 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Mini Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets, can be added along with the protease inhibitor tablets.

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Pierce™ Phosphatase Inhibitor Mini Tablets Thermo Scientific™

Thermo Scientific Pierce Phosphatase Inhibitor Mini Tablets provide complete cell lysate and biological sample protection from dephosphorylation by a broad spectrum of serine and threonine phosphatases.

Features of Pierce Phosphatase Inhibitor Mini Tablets:
Economical—more cost effective than other commercially available formulations
Improved formulation—tablets dissolve quickly to form a clear solution
Complete—the tablets inhibit acid, alkaline, and protein phosphatases
Compatible—use with homebrew or commercially available cell lysis reagents and Thermo Scientific protein assays

Pierce Phosphatase Inhibitor Mini Tablets protect phosphoproteins from serine/threonine phosphatases and tyrosine phosphatases. Each Phosphatase Inhibitor Tablet contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate for broad-spectrum inhibition of phosphatase activity. Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10 mL of solution. The Pierce Phosphatase Inhibitor Tablets are suitable for use with any cell or tissue extract to preserve protein phosphorylation for downstream applications.

Protein phosphorylation and dephosphorylation states often function as molecular on/off switches for key regulators in biological pathways within the cell, including signal transduction, cell division, and apoptosis. To effectively study phosphorylation signaling events, endogenous phosphatases must be inactivated to prevent their uncontrolled activity upon cell lysis. Pierce Phosphatase Inhibitor Tablets preserve phosphoprotein modifications by significantly reducing endogenous acid, alkaline, and protein phosphatase activity.

Pierce Phosphatase Inhibitor Mini Tablets are compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Where incompatibilities arise, the inhibitor components can be removed by dialysis or desalting before performing these procedures.

To use Pierce Phosphatase Inhibitor Mini Tablets, simply dissolve 1 tablet in 10 mL of buffer or lysate. Pierce Phosphatase Inhibitor Mini Tablets do not contain protease inhibitors. If necessary, protease inhibitors, such as Pierce Protease Inhibitor Tablets, can be added along with the phosphatase inhibitor tablet.

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PKI [14 22] Myristoylated PTD Invitrogen™

cAMP-dependent protein kinase (PKA) inhibitor. This peptide is a highly specific inhibitor of cAMP-dependent protein kinase (PKA).

Format/Formulation: Lyophilized trifluoroacetate salt, >97% purity by HPLC.

Molecular Weight: 1208

BacMam Enhancer Kit Invitrogen™

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.

To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells for 1.5–2 hours at 37°C and 5% CO2 and replace with fresh medium. Different cell types vary in their response to the BacMam enhancer and for best results the amount and treatment time will need to be optimized. Once prepared, the enhancer solution is stable to multiple freeze⁄thaw cycles following the recommended storage conditions. If desired, the solution can be divided into aliquots following reconstitution.

Pierce Protease and Phosphatase Inhibitor Mini Tablets Thermo Scientific™

Thermo Scientific Pierce Protease and Phosphatase Inhibitor Mini Tablets contain both protease inhibitors and phosphatase inhibitors to provide broad-spectrum complete protection from dephosphorylation and proteolytic degradation.

Features of Protease and Phosphatase Inhibitor Mini Tablets:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays

The Pierce Protease and Phosphatase Inhibitor Mini Tablets contain aprotinin, bestatin, E-64, EDTA, and leupeptin to provide protection from degradation by serine proteases, cysteine proteases, and aspartic acid proteases. To prevent the dephosphorylation activity of serine/threonine and tyrosine phosphatases, each tablet also contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate. Pierce Protease and Phosphatase Inhibitor Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10 mL of solution.

Pierce Protease and Phosphatase Inhibitor Mini Tablets are the first complete inhibitor tablet. Adding one tablet to 10 mL of lysis buffer provides complete protection from protease and phosphatase activity during protein extraction and protein purification. There are two formulations of this inhibitor available, one containing EDTA and one without. While both formulations have metalloprotease inhibitors, an EDTA-free formulation is available for applications that require divalent cations for protein activity. Similarly, EDTA and certain phosphatase inhibitors interfere with immobilized metal chelate affinity chromatography (IMAC) and 2D electrophoresis. Dialyze or desalt the sample before performing these procedures. The Protease and Phosphatase Inhibitor formulation is mass spectrometry-compatible because it does not contain AEBSF.

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WORTMANNIN Invitrogen™

Wortmannin, a fungal metabolite, is a potent and selective inhibitor of phosphatidylinositol 3-kinase.

Format/Formulation: Lyophilized

RiboLock RNase Inhibitor (40 U/µL) Thermo Scientific™

Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A,B and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

Highlights

Performs under a wide range of reaction conditions
Protects RNA from degradation at temperatures up to 55°C

Note

DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity. Recommended concentration 1 U/ µL of a reaction mixture.

Ribonuclease Inhibitor, Cloned Invitrogen™

Ribonuclease Inhibitor, Cloned is a potent non-competitive inhibitor of neutral pancreatic ribonucleases.

Applications:
Cell-free translation systems (1). Reverse transcription of mRNA (2).

Source:
Recombinant, purified from E. coli.

Performance and Quality Testing:
No detectable contaminating activity is observed in DNA nicking, ribonuclease, and protease assays.

Unit Definition:
One unit inhibits 5 ng RNase A by 50% using cytidine 2´, 3´ cyclic monophosphate (cCMP) as the substrate (3).

IBMX Invitrogen™

IBMX is a nonspecific inhibitor of phosphodiesterases that also possesses adenosine agonist activity.

Format/Formulation: Lyophilized

Pierce Protease Inhibitor Tablets Thermo Scientific™

Thermo Scientific Pierce Protease Inhibitor Tablets contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Features of Protease Inhibitor Tablets:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays

Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A. Stable for storage at 4°C for up to 12 months, each tablet is sufficient for 50 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.

To use Pierce Protease Inhibitor Tablets, simply dissolve 1 tablet in 50 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets, can be added along with the protease inhibitor tablets.

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Pierce Protease Inhibitor Tablets, EDTA-free
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Ambion™ RNase Inhibitor, cloned, 40 U/µL Invitrogen™

Ambion® RNase Inhibitor, a recombinant human protein produced in E. coli, is a potent inhibitor of neutral pancreatic RNase A type enzymes. The mode of inhibition is noncompetitive; the inhibitor tightly binds RNases in a 1:1 ratio. Supplied in one tube containing 10,000 U (40 U/ µL). The enzyme has been shown to inactivate RNases present in many tissues and cell types. Addition of the ribonuclease inhibitor (RI) has been shown to be useful whenever the integrity of RNA must be maintained such as in the preparation of cDNA by reverse transcription, in vitro RNA transcription, and in in vitro protein synthesis. RI does not inhibit RNase I, T1, T2, H, U1, U2, or CL3. RI requires a minimum of 1 mM DTT to maintain activity, and has an activity range between pH 5.0 to 8.0 with maximal activity between pH 7.0 and 8.0. Since the mode of inhibition is the formation of a 1:1 complex with RNases, care must be taken to avoid denaturation or oxidation of the ribonuclease inhibitor (e.g. by addition of SDS, urea, etc.). RI should be added to transcription, translation, and cDNA synthesis reactions to give a final concentration of 1 U/ µL. RNase Inhibitor is rigorously tested for contaminating RNase, exonuclease, endonuclease, and protease activity.

Unit Definition:
One unit is the amount of protein required to inhibit the activity of 5 ng of RNase A by 50%. Unit assay conditions: 100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 1 mM cyclic 2',3'-CMP, and RNase inhibitor. Addition of RNase A initiates the reaction. RNase Inhibitor activity is measured by the inhibition of hydrolysis of cyclic 2', 3'-CMP by RNase A.

Pierce Protease Inhibitor Mini Tablets Thermo Scientific™

Thermo Scientific Pierce Protease Inhibitor Mini Tablets contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Features of Protease Inhibitor Mini Tablets:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays

Pierce Protease Inhibitor Mini Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A. Stable for storage at 4°C for up to 12 months, each mini tablet is sufficient for 10 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.

To use Pierce Protease Inhibitor Mini Tablets, simply dissolve 1 tablet in 10 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Mini Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets, can be added along with the protease inhibitor tablets.

Related products
Pierce Protease Inhibitor Mini Tablets, EDTA-free
Pierce Protease Inhibitor Tablets
Pierce Phosphatase Inhibitor Mini Tablets
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Paclitaxel (Taxol Equivalent) - for use in research only Invitrogen™

Invitrogen offers paclitaxel for research purposes only at a purity of >98% by HPLC. Paclitaxel, formerly referred to as taxol in some scientific literature, is the approved generic name for the anticancer pharmaceutical Taxol (a registered trademark of Bristol-Myers Squibb Co). Paclitaxel promotes tubulin assembly, producing aggregates that cannot be depolymerized by dilution, calcium ions, cold or a number of microtubule-disrupting drugs. Cultured cells treated with paclitaxel are blocked in the G2 and M phases of the cell cycle.

RNaseOUT™ Recombinant Ribonuclease Inhibitor Invitrogen™

RNaseOUT™ Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT™ Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of ~52 kDa. RNaseOUT™ inhibits RNase A, RNase B, and RNase C.

Applications
cDNA synthesis, RT-PCR, and in vitro transcription and translation

Source
Purified by affinity chromatography from E. coli expressing a cloned porcine gene

Performance and quality testing
SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR

Unit definition
One unit inhibits 5 ng of RNase A by 50% using cytidine 2´, 3´ cyclic monophosphate (cCMP) as the substrate

Unit reaction conditions
100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 µg RNase A in 1 mL from 0 to 10 min at 25°C
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