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CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate) (Thermo Scientific™)

Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.

CHAPS is sulfobetaine derivative of cholic acid. This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity. CHAPS detergent is soluble over a wide range of pH (2 to 12) and is easily removed from solution by dialysis because it has a high critical micelle concentration (CMC).

Features of this buffer:

• Versatile zwitterionic detergent
• Provides mild but effective lysis of cultured mammalian cells
• Nondenaturing and generally does not inactivate protein functions
• Can be removed from solution by dialysis
• Commonly used for isoelectric focusing (IEF) and 2D electrophoresis

Applications:

• Solubilizing the opiate receptor from rat brain cell membranes, while retaining the reversible opiate binding property of the receptor 1
• Purifying microsomal signal peptidase as a complex 2
• Solubilizing dopamine D 2,3, muscarinic 4, and opiate receptors 5 from brain tissue

CHAPS has been successfully used to solubilize intrinsic membrane proteins and receptors and maintain the functional capability of the protein of interest. CHAPS has also been used in combination with nonionic detergents such as NP-40 for nondenaturing gel electrophoresis applications. CHAPS has largely replaced NP-40 in isoelectric focusing (IEF) applications, where it prevents streaking in certain pH ranges. The neutral charge of CHAPS and its disaggregating properties are believed to be responsible for the improvement.

Properties of CHAPS Detergent:

• Chemical Name: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate
• Molecular Weight: 614.88g
• Detergent Class: Zwitterionic
• Aggregation Number: 10
• Micelle Molecular Weight: 6149g
• Critical Micelle Concentration (CMC): 8 to 10 mM (0.4920 to 0.6150%, w/v)
• Cloud Point: ≥100°C
• Dialyzable: Yes

Specifications for CHAPS:

• Visual: White free-flowing powder, free of foreign matter.
• Solubility: A 10% (aq) solution is clear, colorless.
• Identity: The I.R. scan must show only peaks characteristic for the structure and functional groups of the compound being tested.
• Purity: (TLC) One major spot. A small, secondary spot is acceptable.

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Surfact-Amps™ Detergent Sampler

Brij™-35, 30% Solution (Thermo Scientific™)

This Thermo Scientific™ formulation of Brij™ 35 is a stabilized 30% (w/v) solution of Brij-35 detergent for use in various protein methods. Brij™ 35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or a surfactant in various HPLC applications.

Properties of Brij 35 Detergent:

• Molecular Weight: 1225g
• Detergent Class: Nonionic
• Aggregation Number: 40
• Micelle Molecular Weight: 49,000g
• Critical Micelle Concentration (CMC): 0.09 mM (0.011%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Features of this Thermo Scientific detergent solution:

Brij 35—a nonionic detergent for use in various protein methods
Accurate—30% detergent solution in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use

Specifications of this Thermo Scientific Brij 35, 30% Solution:

• Visual: Clear, colorless viscous liquid, free of foreign material.
• Concentration: Concentration: 27.0 to 30.0%

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Brij™-35 Surfact-Amps™ (10% w/v) Solution

Brij™-35 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Brij™ 35 Surfact-Amps™ Detergent Solution is a stabilized 10% solution of Brij-35 detergent for use in various protein methods. Thermo Scientific™ Surfact-Amps™ Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability.

Properties of Brij 35 Detergent:

• Molecular Weight: 1225g
• Detergent Class: Nonionic
• Aggregation Number: 40
• Micelle Molecular Weight: 49,000g
• Critical Micelle Concentration (CMC): 0.09 mM (0.011%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

The detergent Brij™ 35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or a surfactant in various HPLC applications. Unlike neat detergents, which are extremely viscous, Surfact-Amps 10% Solutions are easy to pipette and accurately dispense. The surfactant solutions are carefully prepared and packaged under nitrogen in glass ampules or HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of these Brij 35 Surfact-Amps Detergent Solutions:

Brij 35—a nonionic detergent for use in various protein methods
Accurate—10% detergent solutions in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use

Specifications Brij 35 Surfact-Amps Detergent Solutions:

• Visual: Clear, colorless solution, free of particulate matter.
• Concentration: 10.0 ±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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Brij™-35, 30% Solution

Brij™-58 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Thermo Scientific™ Surfact-Amps™ 58 Detergent Solution is highly-purified Brij™ 58 detergent stabilized and supplied as a 10% solution (w/v) in 10 mL glass ampules.

Properties of Brij 58 Detergent:

• Molecular Weight: 1120g
• Detergent Class: Nonionic
• Aggregation Number: 70
• Micelle Molecular Weight: 82,000g
• Critical Micelle Concentration (CMC): 0.077mM (0.0086%, w/v)
• Cloud Point: >100°C

Features of Surfact-Amps 58 Detergent Solution:

Brij 58—a nonionic detergent for use in various protein methods
Accurate—10% detergent solutions in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use

Specifications for Surfact-Amps 58 Detergent Solution:

• Visual: Clear to slightly hazy, colorless solution, free of particulates.
• Concentration: 10.0 ±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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Brij™-35 Surfact-Amps™ (10% w/v) Solution

Tween™ 20 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Thermo Scientific Tween-20 Surfact-Amps Detergent Solution is a highly-purified Tween™ 20 detergent stabilized as a 10% solution (w/v) and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring it's stability and eliminating the accumulation of peroxides and degradation products.

Features of Tween-20 Surfact-Amps Detergent Solution:

Tween 20—a popular nonionic detergent for use in ELISA, Western blotting and other immunoassay PBS or TBS wash buffers
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Properties of Tween 20:
• Molecular Weight: 1228g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: Unknown
• Micelle Molecular Weight: Unknown
• Critical Micelle Concentration (CMC): 0.06 mM (0.0074%, w/v)
• Cloud Point: 95°C
• Dialyzable: No

Specifications for Tween 20 Surfact-Amps Detergent Solution:
• Visual: Clear, light yellow liquid, free of particulates.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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Surfact-Amps™ Detergent Sampler

Surfact-Amps™ Detergent Sampler (Thermo Scientific™)

Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

Features of Surfact-Amps Detergent Sampler are:
• Precise 10% detergent solutions in ultrapure water ampuled under nitrogen
• Easy to accurately dispense and dilute for use
• Exceptionally pure: less than 1.0µeq/mL peroxides and carbonyls
• Highly stable: packaged in glass ampules under inert nitrogen gas

The sampler kit is an economical way to test which detergent is most effective for a particular protein storage or experimental system. Thermo Scientific Surfact-Amps Purified Detergent Solutions are convenient 10% (w/v) solutions of commonly used detergents that provide unsurpassed purity, quality and stability for proteomics and molecular biology methods. Unlike neat detergent formulations, Surfact-Amps 10% Solutions are not so viscous that you can't aliquot them accurately. The detergent solutions are carefully prepared and packaged under nitrogen in glass ampules, ensuring their stability and eliminating the accumulation of peroxides and degradation products. Three of the ten detergents in the sampler kit are supplied as solids (100 mg each) rather than 10% solutions.

Detergents are a class of molecules whose unique properties enable manipulation (disruption or formation) of hydrophobic-hydrophilic interactions among molecules in biological samples. In biological research, detergents are used to lyse cells (release soluble proteins), solubilize membrane proteins and lipids, control protein crystallization, prevent nonspecific binding in affinity purification and immunoassay procedures, and as additives in electrophoresis. Generally, moderate concentrations of mild (i.e., nonionic) detergents compromise the integrity of cell membranes, thereby facilitating lysis of cells and extraction of soluble protein, often in native form. Using other conditions, detergents effectively penetrate between the membrane bilayers at concentrations sufficient to form mixed micelles with isolated phospholipids and membrane proteins.

ZOOM™ CHAPS (Invitrogen™)

The ZOOM® IEF Fractionator offers a fast, reliable method to reduce sample complexity, enrich low abundance proteins, and increase the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM® IEF Fractionator provides reproducible separations in three hours. Fractionated samples are ready for further analysis by two dimensional gel electrophoresis (2DE), one dimensional gel electrophoresis (1DE), or two dimensional liquid chromatography/mass spectrometry (2D LC/MS).

The ZOOM® IEF Fractionator System (Figure 1) consists of the following components:

The ZOOM® IEF Fractionator is built for safety, reliability, and durability. It includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid.
ZOOM® Disks are pre-cast polyacrylamide gels that eliminate the need for manual preparation and minimize any chance of cross contamination. These immobilized buffered disks are pre-labeled, disposable, and designed for single use, ensuring consistent and reproducible fractionation. They resolve samples into as many as 7 fractions (using 8 disks of specific pH) to six fractions (using six disks of specific pH) from pH 3–12. Simply place each disk between the sample chambers in the ZOOM® IEF Fractionator to allow separation in a specific pH range.
ZOOM® Reagents are proteomic grade reagents, including ZOOM® 2D Protein Solubilizers urea, thiourea, CHAPS, and ampholytes, that ensure optimal conditions for sample preparation.
The Basic Protein Kit, which includes ZOOM® Discs and ZOOM Strips for IEF of basic proteins, focusing buffers, and solubilizer kits.

Sample Chamber and Fractionator Unit Set-up Procedure
Figures 4–6 illustrate assembly of the sample chambers and fractionator unit. The sample chamber and components fit together easily and offer a leak-proof seal. Simply place disks between the sample chambers in the ZOOM® IEF Fractionator to allow separation in a specific pH range. For example, if you want to fractionate proteins between pH 4.6 and 5.4, flank a sample chamber with a ZOOM® Disk of pH 4.6 and one of pH 5.4.

Downstream Processing by 2D Gel Electrophoresis
Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM® Strips on the ZOOM® IPGRunner™ System. Follow with second dimension analysis using neutral pH NuPAGE® ZOOM® Gels.

Triton™ X-100 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Thermo Scientific Triton X-100 Surfact-Amps Detergent Solution is a highly-purified Triton™ X-100 detergent stabilized as a 10% solution (w/v) in variable sizes packaged under nitrogen in 10 mL glass ampules or three sizes of non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of Triton X-100 Surfact-Amps Detergent Solution:

Triton X-100—a nonionic detergent for use in various protein methods
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Specifications for Triton X-100 Surfact-Amps Detergent Solution:
• Visual: Clear to slightly hazy, colorless solution, free of particulate matter.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Properties of Triton X-100:
• Molecular Weight: 647g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: 140
• Micelle Molecular Weight: 90,000g
• Critical Micelle Concentration (CMC): 0.24 mM (0.155%, w/v)
• Cloud Point: 64°C
• Dialyzable: No

Triton X-100 is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins.

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Tween™ 80 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Thermo Scientific Tween-80 Surfact-Amps Detergent Solution is a highly-purified Tween™ 80 detergent stabilized as a 10% solution (w/v) and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring it's stability and eliminating the accumulation of peroxides and degradation products.

Features of Tween-80 Surfact-Amps Detergent Solution:

Tween 80—a popular nonionic detergent for use in ELISA, Western blotting and other immunoassay PBS or TBS wash buffers
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Properties of Tween 80:
• Molecular Weight: 1310g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: 60
• Micelle Molecular Weight: 76,000g
• Critical Micelle Concentration (CMC): 0.012 mM (0.0016%, w/v)
• Cloud Point: Unknown
• Dialyzable: No

Specifications for Tween 80 Surfact-Amps Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

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Triton X-100 (1%) (Invitrogen™)

This reagent, a component of the Fetal Hemoglobin (HbF) test, comes as a 1% solution that can be diluted in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and sodium azide to create a 0.1% working solution.

Pluronic™ F-127 (20% Solution in DMSO) (Invitrogen™)

Pluronic F-127 *20% solution in DMSO* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.

Pluronic™ F-127, low UV absorbance (Invitrogen™)

Pluronic F-127 is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media.1 Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.

NP-40 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Thermo Scientific Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized as a 10% solution in your choice of either 10 mL glass ampules or two sizes of HDPE-plastic bottles.

Features of Surfact-Amps NP-40 Detergent Solution:

NP-40—a nonionic detergent for use in various protein methods
Accurate—precise 10% detergent solutions in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules

This is an easy-to-use 10% (w/v) solution of purified NP-40 detergent for use in routine and high-demand protein research methods and molecular biology techniques. NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability. Unlike neat detergents, which are extremely viscous, Surfact-Amps 10% Solutions are easy to pipette and accurately dispense. The surfactant solutions are carefully prepared and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Properties of NP-40 Detergent:
• Molecular Weight: 617g
• Detergent Class: Nonionic
• Aggregation Number: 149
• Micelle Molecular Weight: 90,000g
• Critical Micelle Concentration (CMC): 0.29 mM (0.0179%, w/v)
• Cloud Point: 80°C
• Dialyzable: No

Specifications for NP-40 Surfact-Amps Detergent Solution:
• Visual: Clear, colorless solution, free of particulate matter.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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Sodium Dodecyl Sulfate (SDS), C12 (Thermo Scientific™)

Thermo Scientific Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters.

Features of Sodium Dodecyl Sulfate (C12):

• Popular anionic detergent for protein electrophoresis and solubilization
• Component of many cell lysis buffer formulations
• Specially purified formulation is >98% C12 alkyl sulfate
• Contains low levels of hexadecyl sulfate (C16), which inhibits protein renaturation

Sodium dodecyl sulfate (SDS) is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. It is a component of many cell lysis buffer formulations, including RIPA buffer. Typical SDS or lauryl sulfate preparations are mixtures of several different chain lengths (C10 to C18). However, protein renaturation and other specialized methods require very specific surfactant and buffer conditions. This particular formulation of SDS is highly purified to eliminate nearly all secondary chain lengths besides C12.

Properties of Sodium Dodecyl Sulfate, (pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate (SDS), C12 (Part No. 28312):
• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless and free of particulates.
• Chain length: C12 ≥98.0% if C16 ≤0.8%
• Report C10, C12, C14, C16, C18 concentrations.

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Sodium Dodecyl Sulfate (SDS), Lauryl (Thermo Scientific™)

Thermo Scientific Sodium Dodecyl Sulfate (Lauryl) is standard-grade SDS detergent for use in protein polyacrylamide gel electrophoresis (PAGE).

Features of Sodium Dodecyl Sulfate (Lauryl):

• Popular anionic detergent for a variety of protein methods
• Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE)
• Common component of cell lysis buffers

This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. The formulation is a mixture of several different alkyl sulfate chain lengths (C10 to C18).

Properties of SDS (values for pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate:
• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless to slightly yellow.
• Chain length: C12 >60%; C14 = 20 to 35%; C16 <10%; C10 and C18 <1% each

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